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The map label for this gene is mlp [H]

Identifier: 110834835

GI number: 110834835

Start: 2251683

End: 2253035

Strand: Reverse

Name: mlp [H]

Synonym: ABO_1974

Alternate gene names: 110834835

Gene position: 2253035-2251683 (Counterclockwise)

Preceding gene: 110834838

Following gene: 110834834

Centisome position: 72.21

GC content: 56.61

Gene sequence:

>1353_bases
ATGCATATTCATATTCTCGGTATTTGCGGCACCTTTATGGGGTCGCTGGCGCAGCTGGCTAAAGCTCAGGGGCATCAGGT
TACTGGCAGTGACCAAAACGTTTACCCGCCCATGAGCGATCAATTGGAAGCGGCGGGTATTGCCTTGACCCAAGGGTATG
ATCCGTCGCAACTGGAACCGGCGCCGGATCTAGTGGTTATCGGCAATGCGTTAAGCCGTGGCAACCCGGCAGTGGAAGCA
GTACTGGATCGCGGCTTACCCTATACCTCAGGGGCGCAACTGCTCCAAGAGGTGGTGCTTCCCGGTCGCTGGGTGCTAGC
TGTTGCCGGAACCCATGGTAAAACCACCACCAGTTCCATGCTGGCCTGGATCTTGGAATATGCGGGTTTAAAGCCTGGTT
ATTTGATTGGCGGTGTACCCGGCAATTTCGGAGTATCGGCCCGGTTGGGTGAGACGCCTTTCTTTGTGGTGGAAGCAGAC
GAATACGATACGGCGTTTTTTGACAAGCGCAGCAAGTTTGTCCACTACCGTCCGCGCACGGTAATTCTTAATAATTTGGA
ATTTGATCACGCGGATATTTTTGCGGATCTGGCCGCTATTCAAACCCAGTTCCATCACCTGATTCGTACTGTGCCGTCCA
CCGGTCGCTTGATTCTGCCCCATGGAGAAGCTGCGCTGGAGGAGACCCTGGAAAAAGGCTGCTGGAGTAACGTGGAGCGC
TTTGGTAAAGAGGAAGCGCTGGGCTACCGCCTGGATCAGGCCGATGGCTCGAGCTTTACGGTGCTTGAAAGCGGTGAACC
GGTGGCGCAGGTGCGTTGGGAGCAAACTGGCCGTCACAGTGTGAACAACGGAATGGCTGCGTTGCTGGCCGCCCGCCATG
TGGGTGTGACTTTGGCGGATGGCGCGGCAGCGTTATCGGAGTTTGTGGGTGTGAAGCGGCGCATGGAATTGCGTGGTGAA
GTGGGCGGGGTGCGGGTTTACGACGATTTTGCCCATCACCCCACGGCCATTGCCACCACGCTAGATGGACTGCGGCGCAA
GGTGGGTAACGGCAAAATACTTGCCATTATTGAGCCTCGCTCTAATACCATGCGCCTAGGGAGCCACAAGGCCGCTTTGG
CGGGCAGCACCGGATGCGCTGATGAGGTAATCTGGTATCAGCCGCCGGGCTTGAACTGGTCGCTGGATTCGGTGCTCAGT
GAAGCTCCGGTGCCGGCAACGGTGACCGAATCGCTGGATGATATTATTGCCAAGGTAGCAAGTCACAAAGGCGAGCCACT
GAGCGTGGTGGTGATGAGCAACGGCGGCTTTGGTGGCATTCACCAGAAGCTGCTGGATGCGTTGGCTGAGTAA

Upstream 100 bases:

>100_bases
TAGACAATAGGTTCAACAGGGATTAGACCGTGCTGGCACTTCACCTTGGGAGCCCTTGCCGTTATGGTGCAAGGAATCTA
AATGTTGGCAGTTCCTTCTC

Downstream 100 bases:

>100_bases
CAGCGACAGACAAGCCTCACGTTGCTGCAAATGATAGTAGTAAGCGTCTTGACGCGTCTGTGCCCGCGCTTTTGTCTTGA
GTGCCGGGAGAAAATTGCAA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-m eso-diamino

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MHIHILGICGTFMGSLAQLAKAQGHQVTGSDQNVYPPMSDQLEAAGIALTQGYDPSQLEPAPDLVVIGNALSRGNPAVEA
VLDRGLPYTSGAQLLQEVVLPGRWVLAVAGTHGKTTTSSMLAWILEYAGLKPGYLIGGVPGNFGVSARLGETPFFVVEAD
EYDTAFFDKRSKFVHYRPRTVILNNLEFDHADIFADLAAIQTQFHHLIRTVPSTGRLILPHGEAALEETLEKGCWSNVER
FGKEEALGYRLDQADGSSFTVLESGEPVAQVRWEQTGRHSVNNGMAALLAARHVGVTLADGAAALSEFVGVKRRMELRGE
VGGVRVYDDFAHHPTAIATTLDGLRRKVGNGKILAIIEPRSNTMRLGSHKAALAGSTGCADEVIWYQPPGLNWSLDSVLS
EAPVPATVTESLDDIIAKVASHKGEPLSVVVMSNGGFGGIHQKLLDALAE

Sequences:

>Translated_450_residues
MHIHILGICGTFMGSLAQLAKAQGHQVTGSDQNVYPPMSDQLEAAGIALTQGYDPSQLEPAPDLVVIGNALSRGNPAVEA
VLDRGLPYTSGAQLLQEVVLPGRWVLAVAGTHGKTTTSSMLAWILEYAGLKPGYLIGGVPGNFGVSARLGETPFFVVEAD
EYDTAFFDKRSKFVHYRPRTVILNNLEFDHADIFADLAAIQTQFHHLIRTVPSTGRLILPHGEAALEETLEKGCWSNVER
FGKEEALGYRLDQADGSSFTVLESGEPVAQVRWEQTGRHSVNNGMAALLAARHVGVTLADGAAALSEFVGVKRRMELRGE
VGGVRVYDDFAHHPTAIATTLDGLRRKVGNGKILAIIEPRSNTMRLGSHKAALAGSTGCADEVIWYQPPGLNWSLDSVLS
EAPVPATVTESLDDIIAKVASHKGEPLSVVVMSNGGFGGIHQKLLDALAE
>Mature_450_residues
MHIHILGICGTFMGSLAQLAKAQGHQVTGSDQNVYPPMSDQLEAAGIALTQGYDPSQLEPAPDLVVIGNALSRGNPAVEA
VLDRGLPYTSGAQLLQEVVLPGRWVLAVAGTHGKTTTSSMLAWILEYAGLKPGYLIGGVPGNFGVSARLGETPFFVVEAD
EYDTAFFDKRSKFVHYRPRTVILNNLEFDHADIFADLAAIQTQFHHLIRTVPSTGRLILPHGEAALEETLEKGCWSNVER
FGKEEALGYRLDQADGSSFTVLESGEPVAQVRWEQTGRHSVNNGMAALLAARHVGVTLADGAAALSEFVGVKRRMELRGE
VGGVRVYDDFAHHPTAIATTLDGLRRKVGNGKILAIIEPRSNTMRLGSHKAALAGSTGCADEVIWYQPPGLNWSLDSVLS
EAPVPATVTESLDDIIAKVASHKGEPLSVVVMSNGGFGGIHQKLLDALAE

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=59.8669623059867, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1786279, Length=376, Percent_Identity=30.0531914893617, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48138; Mature: 48138

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGSLAQLAKAQGHQVTGSDQNVYPPMSDQLEAAGIALTQGYDPSQLEP
CEEEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHCCEEEECCCCCCCCCC
APDLVVIGNALSRGNPAVEAVLDRGLPYTSGAQLLQEVVLPGRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LAWILEYAGLKPGYLIGGVPGNFGVSARLGETPFFVVEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHHCCCCEEEECCCE
VILNNLEFDHADIFADLAAIQTQFHHLIRTVPSTGRLILPHGEAALEETLEKGCWSNVER
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
FGKEEALGYRLDQADGSSFTVLESGEPVAQVRWEQTGRHSVNNGMAALLAARHVGVTLAD
HCCHHHCCEEEECCCCCEEEEEECCCCCEEEEHHHCCCCCHHCHHHHHHHHHHCCEEEEC
GAAALSEFVGVKRRMELRGEVGGVRVYDDFAHHPTAIATTLDGLRRKVGNGKILAIIEPR
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SNTMRLGSHKAALAGSTGCADEVIWYQPPGLNWSLDSVLSEAPVPATVTESLDDIIAKVA
CCCEECCCCCHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
SHKGEPLSVVVMSNGGFGGIHQKLLDALAE
HCCCCCEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGSLAQLAKAQGHQVTGSDQNVYPPMSDQLEAAGIALTQGYDPSQLEP
CEEEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHCCEEEECCCCCCCCCC
APDLVVIGNALSRGNPAVEAVLDRGLPYTSGAQLLQEVVLPGRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LAWILEYAGLKPGYLIGGVPGNFGVSARLGETPFFVVEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHHCCCCEEEECCCE
VILNNLEFDHADIFADLAAIQTQFHHLIRTVPSTGRLILPHGEAALEETLEKGCWSNVER
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
FGKEEALGYRLDQADGSSFTVLESGEPVAQVRWEQTGRHSVNNGMAALLAARHVGVTLAD
HCCHHHCCEEEECCCCCEEEEEECCCCCEEEEHHHCCCCCHHCHHHHHHHHHHCCEEEEC
GAAALSEFVGVKRRMELRGEVGGVRVYDDFAHHPTAIATTLDGLRRKVGNGKILAIIEPR
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SNTMRLGSHKAALAGSTGCADEVIWYQPPGLNWSLDSVLSEAPVPATVTESLDDIIAKVA
CCCEECCCCCHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
SHKGEPLSVVVMSNGGFGGIHQKLLDALAE
HCCCCCEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]