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The map label for this gene is lasT [H]

Identifier: 110834589

GI number: 110834589

Start: 1967187

End: 1967858

Strand: Direct

Name: lasT [H]

Synonym: ABO_1728

Alternate gene names: 110834589

Gene position: 1967187-1967858 (Clockwise)

Preceding gene: 110834588

Following gene: 110834590

Centisome position: 63.05

GC content: 56.85

Gene sequence:

>672_bases
ATGGATATTGTTTTTATTCTGGTGGAACCCGCTCGCGGGGAAAACATTGGCGCCGCAGCCCGTGCTATTAAAACCATGGG
CTTCTCCCAGTTACGGCTGGTAAACCCCGGCGATATGACCGCCGCCCACTGGGTGGCGCACCACAGCAACGACGTGCTGG
AACAAGCCATGATCGTGGATTCCTTGGCGCAGGCCCTGGTTGATGTAGACCTAAGCATCGGCTGCACCGCCCGGCACCGC
TTGCAAAAAGATCGCTACGTGGATGCAGATCACTGTGCCGCACTGGTGGCCGGCAAGGGAAACAGCGTACAGCGGGTGGC
TATCGTTTTTGGCCGGGAAGCCAGCGGTCTCACCGGCGACGAGCTGGGCCTTTGCGACTTGGCTAGCAGTATTCCACTGG
CGCAGCAGCAGCCATCGCTGAATCTCGCGCAGGCGGTAATGATCTACGCTTGGGAATTGCGCAAGCAACCCGCTGTAAAA
ATAACAGAACCGGACCCGGCCACTTTTTCCCATACGCGTCAGGCAGTGCATCAGCAGCTTGCCTCGTTGCACATACAACC
CAATGAAAACCTGCACCAATGGGTAGATGAATTACTACCGCTGGCTAACGACCGGGAACTGGGCCTGGTTCGCCAATTAC
TTCGCCGATTATCATCGCAGGGAAAGCAATAG

Upstream 100 bases:

>100_bases
CTTTATCCCCCCTCTATCTACAACCCTCTTTTCTGCAAACCGTACCAACGGCTCGTATAGAATGGTTCGCGTTACACACA
GCCTGAACAAGTGACTATAA

Downstream 100 bases:

>100_bases
CCCCGACCCTGTGATACAAAGATACCCATACTTTCCAAGAGCCCTCCTTATTATGGATCGACGCCTGCCTCTCGTCTTGC
TGATATTGCTGCCCGTTCTC

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDIVFILVEPARGENIGAAARAIKTMGFSQLRLVNPGDMTAAHWVAHHSNDVLEQAMIVDSLAQALVDVDLSIGCTARHR
LQKDRYVDADHCAALVAGKGNSVQRVAIVFGREASGLTGDELGLCDLASSIPLAQQQPSLNLAQAVMIYAWELRKQPAVK
ITEPDPATFSHTRQAVHQQLASLHIQPNENLHQWVDELLPLANDRELGLVRQLLRRLSSQGKQ

Sequences:

>Translated_223_residues
MDIVFILVEPARGENIGAAARAIKTMGFSQLRLVNPGDMTAAHWVAHHSNDVLEQAMIVDSLAQALVDVDLSIGCTARHR
LQKDRYVDADHCAALVAGKGNSVQRVAIVFGREASGLTGDELGLCDLASSIPLAQQQPSLNLAQAVMIYAWELRKQPAVK
ITEPDPATFSHTRQAVHQQLASLHIQPNENLHQWVDELLPLANDRELGLVRQLLRRLSSQGKQ
>Mature_223_residues
MDIVFILVEPARGENIGAAARAIKTMGFSQLRLVNPGDMTAAHWVAHHSNDVLEQAMIVDSLAQALVDVDLSIGCTARHR
LQKDRYVDADHCAALVAGKGNSVQRVAIVFGREASGLTGDELGLCDLASSIPLAQQQPSLNLAQAVMIYAWELRKQPAVK
ITEPDPATFSHTRQAVHQQLASLHIQPNENLHQWVDELLPLANDRELGLVRQLLRRLSSQGKQ

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=219, Percent_Identity=39.2694063926941, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1788881, Length=156, Percent_Identity=40.3846153846154, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24412; Mature: 24412

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVFILVEPARGENIGAAARAIKTMGFSQLRLVNPGDMTAAHWVAHHSNDVLEQAMIVD
CCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHH
SLAQALVDVDLSIGCTARHRLQKDRYVDADHCAALVAGKGNSVQRVAIVFGREASGLTGD
HHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHEEEECCCCCCEEEEEEECCCCCCCCCC
ELGLCDLASSIPLAQQQPSLNLAQAVMIYAWELRKQPAVKITEPDPATFSHTRQAVHQQL
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
ASLHIQPNENLHQWVDELLPLANDRELGLVRQLLRRLSSQGKQ
HHEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDIVFILVEPARGENIGAAARAIKTMGFSQLRLVNPGDMTAAHWVAHHSNDVLEQAMIVD
CCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHH
SLAQALVDVDLSIGCTARHRLQKDRYVDADHCAALVAGKGNSVQRVAIVFGREASGLTGD
HHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHEEEECCCCCCEEEEEEECCCCCCCCCC
ELGLCDLASSIPLAQQQPSLNLAQAVMIYAWELRKQPAVKITEPDPATFSHTRQAVHQQL
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
ASLHIQPNENLHQWVDELLPLANDRELGLVRQLLRRLSSQGKQ
HHEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]