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The map label for this gene is slt [H]

Identifier: 110834448

GI number: 110834448

Start: 1810338

End: 1812281

Strand: Direct

Name: slt [H]

Synonym: ABO_1587

Alternate gene names: 110834448

Gene position: 1810338-1812281 (Clockwise)

Preceding gene: 110834446

Following gene: 110834451

Centisome position: 58.02

GC content: 56.58

Gene sequence:

>1944_bases
ATGGGTCGCACTGCGCCATTAACTCGCGTTGCACTGTTTGCCTGCTGTCTGTTTTCTTCGTTGGTTGTTGGCGCTACGGA
TAGCGGCAACTTCCATCAAGCTCTGGACGCAGCACGCAACGATGACTGGGAAACGCTTTCCCGGCAGCAACAGGCTCTGG
GTGATCAGCACCCTCTGCAGGCCTACCTGGACTTCCACAAACTGCGCGCAGCTTTGCCCCAACTCAGCGCGGACAAAGTA
ATCCAGTACGCTGAACAATACCCGGATTCGCCGCTACCCAAGGATATTCGGCAACTAGCCATTGTCGCTTACGGCAAGGC
AGAAAAATGGCAAGCGCTGCTAGCCATTAGCGACAAAGCTCCCAGTGGCATAGCCCTGCGTTGCTATTACCATCAAGCAC
AATGGCAAACCGGTGACAAAACCGCCCTGCAAGCCGCCAAAGATCTGTGGGCCACCGGCCAGTCCCGGCCTAACAATTGC
GACCCGCTGTTCAACGCCGCTCGAGAGGCCGGAGTCATCGGTGGCCGACAGATCTGGGAACGTCAACAGCTTGCCTTTGA
TGCTAATAATCCCGGACTAATACGCTACTTAGACAAAATGCTAGCAGACAGCGACTACACCAGCGCCAGACAATGGCTGC
AAAAGCTCTACCAAAGCCCCCACAACGTGACCGAGGTGCCTCGCAGCCTTCCTGACACTCAACGCCAGACACTGCTGTCC
GCCGGCTTACGGCGCTGGGCGTATAAAGATACTCAAGCGGCGCGCAAATGGTTCGATACCCAAAGCCGTCAACAGGGCCT
AACAGACAAGACACTACGGGAGCGGGCTGGCAGCCGGATAGCCTGGTACTCCACTATCCGCGGAGTGGAAAGCAACCGCC
AATGGTTAGATAACTGGTTAGCCAATCATCCCGACACCTCGCTGGTGACACAGCGGGCTCGTCGCACTGTAATTGAACAA
GACTGGGACGCGCTAGATGAATGGGTTATGCGCCTACCCGAGGAAGACCGTAATGATTCGCGCTGGTTATACTGGCGAGC
CCGTGCGTTAGAAAGCACCGATCGGCCAGATCAAGCCCGTGAGCTGTATCAACAAGCCGCCCAGCAACGCAATTTCTTTG
CCTTCCTGGCCGCAGACCGGCTGGGTGAACCGTATCGCTTCTCCGAACAGGAGCACCGCTCCTCAGCCACTTTCACCCCA
CCGCCTGCCATCACCCGTATCCGCATGCTACGGGAGCTGAATGAGCCTTACCTGGCGCACAAAGAGTGGATGTGGTTGAT
GTGGCATAGCAATAAGGCACAAATTGCCGATCTGGCAGACTACGCATTGGCTCAAAAATGGTACGACCTGTCTGTGCAGG
CCTCTATCCAGGCCAAGGCCTGGAATACACTGGCCTGGCGCTTCCCACCCGCGTATCTGTCATTATTTGAAGAGCAAGCC
AAACCCCACGGCTTGGATCCCTGGCTCTCTATGGCCGTGGCCCGTCGCGAGAGCGCCTTCAATCCTCAGGCCCGCTCACC
GGTGGGCGCCATGGGGCTCATGCAATTGATGCCGGCCACCGCCCGCAAGGTGGCAAAAGACGCCGAGATGGGCACGCCCA
CCAGCAAAACCCTGCAAGACAAACACACCAATATCGCCCTTGGTAGCCGTTACTTAGCGGAACTCCTTGACGATTTCAGC
GATAACCGTGTGTTGGCTCTAGCGGCTTATAATGCAGGCCCCAGCCGGGTGAACCGCTGGTTGGAAAATGCGGAAGACGG
CGTGTCCGCCGATGTATGGATCGAGTCCATTCCTTTCCGCGAAACCCGGGATTATGTCCAGGCAGTGCTCACCTATCGTG
CTTTATTTGTGGGTATGCACGATGACCAAGCACGCAGTAAACAGCTGCTAACCAGTGACGAGAATGACGAGCTCTATTCC
TTGAGCATGCTGGACCAGGACTGA

Upstream 100 bases:

>100_bases
CATAACGAGAATTCTCCTCAAGTTTTACATAATCACCACGCCAAGCGCACAGTCAGCCCGGTAGAATTGCCCCATTCATT
CCAACGATGTGGAGGCACAA

Downstream 100 bases:

>100_bases
TCGTAAAGCCGAATCAGTCCAGTTGGCGGATGTGGTAATCCAGCCGCGCCGCCGCTTTCGCATGGCTCAGCGCCGGGGTT
ACCGCAATGCGCCGCCGCAG

Product: soluble lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MGRTAPLTRVALFACCLFSSLVVGATDSGNFHQALDAARNDDWETLSRQQQALGDQHPLQAYLDFHKLRAALPQLSADKV
IQYAEQYPDSPLPKDIRQLAIVAYGKAEKWQALLAISDKAPSGIALRCYYHQAQWQTGDKTALQAAKDLWATGQSRPNNC
DPLFNAAREAGVIGGRQIWERQQLAFDANNPGLIRYLDKMLADSDYTSARQWLQKLYQSPHNVTEVPRSLPDTQRQTLLS
AGLRRWAYKDTQAARKWFDTQSRQQGLTDKTLRERAGSRIAWYSTIRGVESNRQWLDNWLANHPDTSLVTQRARRTVIEQ
DWDALDEWVMRLPEEDRNDSRWLYWRARALESTDRPDQARELYQQAAQQRNFFAFLAADRLGEPYRFSEQEHRSSATFTP
PPAITRIRMLRELNEPYLAHKEWMWLMWHSNKAQIADLADYALAQKWYDLSVQASIQAKAWNTLAWRFPPAYLSLFEEQA
KPHGLDPWLSMAVARRESAFNPQARSPVGAMGLMQLMPATARKVAKDAEMGTPTSKTLQDKHTNIALGSRYLAELLDDFS
DNRVLALAAYNAGPSRVNRWLENAEDGVSADVWIESIPFRETRDYVQAVLTYRALFVGMHDDQARSKQLLTSDENDELYS
LSMLDQD

Sequences:

>Translated_647_residues
MGRTAPLTRVALFACCLFSSLVVGATDSGNFHQALDAARNDDWETLSRQQQALGDQHPLQAYLDFHKLRAALPQLSADKV
IQYAEQYPDSPLPKDIRQLAIVAYGKAEKWQALLAISDKAPSGIALRCYYHQAQWQTGDKTALQAAKDLWATGQSRPNNC
DPLFNAAREAGVIGGRQIWERQQLAFDANNPGLIRYLDKMLADSDYTSARQWLQKLYQSPHNVTEVPRSLPDTQRQTLLS
AGLRRWAYKDTQAARKWFDTQSRQQGLTDKTLRERAGSRIAWYSTIRGVESNRQWLDNWLANHPDTSLVTQRARRTVIEQ
DWDALDEWVMRLPEEDRNDSRWLYWRARALESTDRPDQARELYQQAAQQRNFFAFLAADRLGEPYRFSEQEHRSSATFTP
PPAITRIRMLRELNEPYLAHKEWMWLMWHSNKAQIADLADYALAQKWYDLSVQASIQAKAWNTLAWRFPPAYLSLFEEQA
KPHGLDPWLSMAVARRESAFNPQARSPVGAMGLMQLMPATARKVAKDAEMGTPTSKTLQDKHTNIALGSRYLAELLDDFS
DNRVLALAAYNAGPSRVNRWLENAEDGVSADVWIESIPFRETRDYVQAVLTYRALFVGMHDDQARSKQLLTSDENDELYS
LSMLDQD
>Mature_646_residues
GRTAPLTRVALFACCLFSSLVVGATDSGNFHQALDAARNDDWETLSRQQQALGDQHPLQAYLDFHKLRAALPQLSADKVI
QYAEQYPDSPLPKDIRQLAIVAYGKAEKWQALLAISDKAPSGIALRCYYHQAQWQTGDKTALQAAKDLWATGQSRPNNCD
PLFNAAREAGVIGGRQIWERQQLAFDANNPGLIRYLDKMLADSDYTSARQWLQKLYQSPHNVTEVPRSLPDTQRQTLLSA
GLRRWAYKDTQAARKWFDTQSRQQGLTDKTLRERAGSRIAWYSTIRGVESNRQWLDNWLANHPDTSLVTQRARRTVIEQD
WDALDEWVMRLPEEDRNDSRWLYWRARALESTDRPDQARELYQQAAQQRNFFAFLAADRLGEPYRFSEQEHRSSATFTPP
PAITRIRMLRELNEPYLAHKEWMWLMWHSNKAQIADLADYALAQKWYDLSVQASIQAKAWNTLAWRFPPAYLSLFEEQAK
PHGLDPWLSMAVARRESAFNPQARSPVGAMGLMQLMPATARKVAKDAEMGTPTSKTLQDKHTNIALGSRYLAELLDDFSD
NRVLALAAYNAGPSRVNRWLENAEDGVSADVWIESIPFRETRDYVQAVLTYRALFVGMHDDQARSKQLLTSDENDELYSL
SMLDQD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=627, Percent_Identity=33.1738437001595, Blast_Score=312, Evalue=5e-86,
Organism=Escherichia coli, GI87082191, Length=121, Percent_Identity=34.7107438016529, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74074; Mature: 73943

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTAPLTRVALFACCLFSSLVVGATDSGNFHQALDAARNDDWETLSRQQQALGDQHPLQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
AYLDFHKLRAALPQLSADKVIQYAEQYPDSPLPKDIRQLAIVAYGKAEKWQALLAISDKA
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHEEEEECCCC
PSGIALRCYYHQAQWQTGDKTALQAAKDLWATGQSRPNNCDPLFNAAREAGVIGGRQIWE
CCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
RQQLAFDANNPGLIRYLDKMLADSDYTSARQWLQKLYQSPHNVTEVPRSLPDTQRQTLLS
HHHHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
AGLRRWAYKDTQAARKWFDTQSRQQGLTDKTLRERAGSRIAWYSTIRGVESNRQWLDNWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEHHHHHHCCCCHHHHHHHHH
ANHPDTSLVTQRARRTVIEQDWDALDEWVMRLPEEDRNDSRWLYWRARALESTDRPDQAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCCHHHH
ELYQQAAQQRNFFAFLAADRLGEPYRFSEQEHRSSATFTPPPAITRIRMLRELNEPYLAH
HHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHCC
KEWMWLMWHSNKAQIADLADYALAQKWYDLSVQASIQAKAWNTLAWRFPPAYLSLFEEQA
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCEEEEECCHHHHHHHHHHC
KPHGLDPWLSMAVARRESAFNPQARSPVGAMGLMQLMPATARKVAKDAEMGTPTSKTLQD
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KHTNIALGSRYLAELLDDFSDNRVLALAAYNAGPSRVNRWLENAEDGVSADVWIESIPFR
HCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHEECCCCH
ETRDYVQAVLTYRALFVGMHDDQARSKQLLTSDENDELYSLSMLDQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
GRTAPLTRVALFACCLFSSLVVGATDSGNFHQALDAARNDDWETLSRQQQALGDQHPLQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
AYLDFHKLRAALPQLSADKVIQYAEQYPDSPLPKDIRQLAIVAYGKAEKWQALLAISDKA
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHEEEEECCCC
PSGIALRCYYHQAQWQTGDKTALQAAKDLWATGQSRPNNCDPLFNAAREAGVIGGRQIWE
CCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
RQQLAFDANNPGLIRYLDKMLADSDYTSARQWLQKLYQSPHNVTEVPRSLPDTQRQTLLS
HHHHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
AGLRRWAYKDTQAARKWFDTQSRQQGLTDKTLRERAGSRIAWYSTIRGVESNRQWLDNWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEHHHHHHCCCCHHHHHHHHH
ANHPDTSLVTQRARRTVIEQDWDALDEWVMRLPEEDRNDSRWLYWRARALESTDRPDQAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCCHHHH
ELYQQAAQQRNFFAFLAADRLGEPYRFSEQEHRSSATFTPPPAITRIRMLRELNEPYLAH
HHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHCC
KEWMWLMWHSNKAQIADLADYALAQKWYDLSVQASIQAKAWNTLAWRFPPAYLSLFEEQA
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCEEEEECCHHHHHHHHHHC
KPHGLDPWLSMAVARRESAFNPQARSPVGAMGLMQLMPATARKVAKDAEMGTPTSKTLQD
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KHTNIALGSRYLAELLDDFSDNRVLALAAYNAGPSRVNRWLENAEDGVSADVWIESIPFR
HCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHEECCCCH
ETRDYVQAVLTYRALFVGMHDDQARSKQLLTSDENDELYSLSMLDQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]