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The map label for this gene is purN [H]

Identifier: 110834435

GI number: 110834435

Start: 1797895

End: 1798536

Strand: Direct

Name: purN [H]

Synonym: ABO_1574

Alternate gene names: 110834435

Gene position: 1797895-1798536 (Clockwise)

Preceding gene: 110834434

Following gene: 110834436

Centisome position: 57.62

GC content: 55.3

Gene sequence:

>642_bases
GTGACTCACCGGCTAGCGGTATTGATTAGCGGCTCAGGCACCAACCTGCAAGCAATCATGGATGCCCGTGAACACGGCAG
TCTCGATGTCGAGATTGCCGTGGTATTCTCCAATCGAGCCAACGCGGCAGGCTTGGAGCGAGCGTCCCAGGCGGGAATTC
CCACCGCCACTCTGGATCACCGTGACTACCCGAGCAGGGAAGAGTTCGACCAGGCCATGATCGACCTGCTCACCCCGTAT
GCTCCAGACACCGTAGTGCTGGCCGGCTTTATGCGTATTCTCTCTAGTGTTTTTGTACGCCATTACGCCGGGCGCTTAAT
CAACATCCATCCCTCGCTGCTGCCCAAGTATCGGGGGTTAAATACGCATGCTCGTGCCCTGGAAGCCGGCGATAGCGAAC
ATGGCTGCTCCATTCATTTTGTCACCGAAGAACTGGATGGCGGCCCTCTGATTGCACAGGCCCCCATTTCGGTACAAACG
AACGATACCGTTGATTCCCTGTCCAAACGTGTACAACAACGCGAACACCGGCTCTATCCTCAGGTGTTGCAATGGCGTGC
ACAAAATAGACTGGAACTGACCTACAACGGAGTGTTACTGGATGGCAAGCCCCTCCCTACACAGGGTTATATCCTCACTT
AA

Upstream 100 bases:

>100_bases
CTCTCGCCCTGCTGGAAGCAGAAGGTGAAAGCGCCTTCTTGATCGGTGAAATTCAGGCAGGCGACGGCCAGCCCGACGTC
GTTCTGGACGGGCTGTCAGC

Downstream 100 bases:

>100_bases
CCCACTTATTCTCGCCAGTATTATGCTGGTGAGCCTGACGTTCTCTTCCGTCGCCAGCGCAGAGCAAACGCCGTCCCCCT
TTAATTTGGATTACCGCCTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTHRLAVLISGSGTNLQAIMDAREHGSLDVEIAVVFSNRANAAGLERASQAGIPTATLDHRDYPSREEFDQAMIDLLTPY
APDTVVLAGFMRILSSVFVRHYAGRLINIHPSLLPKYRGLNTHARALEAGDSEHGCSIHFVTEELDGGPLIAQAPISVQT
NDTVDSLSKRVQQREHRLYPQVLQWRAQNRLELTYNGVLLDGKPLPTQGYILT

Sequences:

>Translated_213_residues
MTHRLAVLISGSGTNLQAIMDAREHGSLDVEIAVVFSNRANAAGLERASQAGIPTATLDHRDYPSREEFDQAMIDLLTPY
APDTVVLAGFMRILSSVFVRHYAGRLINIHPSLLPKYRGLNTHARALEAGDSEHGCSIHFVTEELDGGPLIAQAPISVQT
NDTVDSLSKRVQQREHRLYPQVLQWRAQNRLELTYNGVLLDGKPLPTQGYILT
>Mature_212_residues
THRLAVLISGSGTNLQAIMDAREHGSLDVEIAVVFSNRANAAGLERASQAGIPTATLDHRDYPSREEFDQAMIDLLTPYA
PDTVVLAGFMRILSSVFVRHYAGRLINIHPSLLPKYRGLNTHARALEAGDSEHGCSIHFVTEELDGGPLIAQAPISVQTN
DTVDSLSKRVQQREHRLYPQVLQWRAQNRLELTYNGVLLDGKPLPTQGYILT

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=46.9072164948454, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=46.9072164948454, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=46.9072164948454, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1788846, Length=206, Percent_Identity=57.7669902912621, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1787483, Length=166, Percent_Identity=34.3373493975904, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=37.037037037037, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=32.994923857868, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582400, Length=182, Percent_Identity=47.2527472527472, Blast_Score=183, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23519; Mature: 23388

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRLAVLISGSGTNLQAIMDAREHGSLDVEIAVVFSNRANAAGLERASQAGIPTATLDH
CCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEECCC
RDYPSREEFDQAMIDLLTPYAPDTVVLAGFMRILSSVFVRHYAGRLINIHPSLLPKYRGL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCC
NTHARALEAGDSEHGCSIHFVTEELDGGPLIAQAPISVQTNDTVDSLSKRVQQREHRLYP
CHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHH
QVLQWRAQNRLELTYNGVLLDGKPLPTQGYILT
HHHHHHCCCCEEEEECCEEECCCCCCCCCEEEC
>Mature Secondary Structure 
THRLAVLISGSGTNLQAIMDAREHGSLDVEIAVVFSNRANAAGLERASQAGIPTATLDH
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEECCC
RDYPSREEFDQAMIDLLTPYAPDTVVLAGFMRILSSVFVRHYAGRLINIHPSLLPKYRGL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCC
NTHARALEAGDSEHGCSIHFVTEELDGGPLIAQAPISVQTNDTVDSLSKRVQQREHRLYP
CHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHH
QVLQWRAQNRLELTYNGVLLDGKPLPTQGYILT
HHHHHHCCCCEEEEECCEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]