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The map label for this gene is yjbN [H]

Identifier: 110834374

GI number: 110834374

Start: 1728980

End: 1730026

Strand: Direct

Name: yjbN [H]

Synonym: ABO_1513

Alternate gene names: 110834374

Gene position: 1728980-1730026 (Clockwise)

Preceding gene: 110834371

Following gene: 110834375

Centisome position: 55.41

GC content: 56.16

Gene sequence:

>1047_bases
TTGCCCCCGCACGCCGCAAACCCCAATCACCCGTTCGACACCGTGTTAATGAGCAACCCACTCTCCACTCAGCTACCGCA
CCAGCCATTGGACCGCCGCCTCAGCGTCGCCCCGATGATGGACTGGACAACCCGCGACTACCGCTTTCTGGCCCGACTGA
TCACTCGCCATACGTTGCTCTACACCGAAATGGTGGTGGCCCAAGCTATTTTACACGGTGACCGTGACCGGTTTTTAGAT
TTCAACGACGAAGAGCACCCCGTCGCCCTGCAACTAGGAGGCAGCGACCCACAACAATTAGCCCAAGCCACGCGCATTGC
CGGGGATTACGGCTATGACGAGATCAATCTGAATGTGGGGTGTCCGTCAGACCGGGTTCAGCAAGGCAAGATCGGGGCCA
TTCTTATGGCAGAACCTGAGCTGGTAGCACGCTGTGTAGAGGCCATGCAGGCGGAGGTTACTGTGCCGGTTACGGTGAAG
ACCCGTATCGGTATCGATGACCAGGATGATTATGATTTTCTCTACCGCTTTGTTGATCGCATGGAAGCCTCAGGCTGCAC
TAGCCTGACCATCCACGCCCGCAAAGCAATTCTTAGCGGCCTCTCGCCCAAAGAAAACAGAGATATTCCGCCGTTAATCT
ACCAACGTGCCTATGCCATCAAACACGCTTTCCCACATATCGAAATCATACTCAACGGTGGCGTAAAGACCCTGAATGAA
GTACACGAACACATGGGTGAAGTGGATGGCGTGATGATTGGTCGCGAGGCCTACCAAAACCCCTATTTTCTAGCCGAGGC
CGACCGGGTTGTGTTTGGCGACGATCACGCCATCCCCAGCCGCCAGACTATTGCTGAGCAGTTTCTACCTTATGTGGAGC
GGCGCTATGCGCTTGGCCATGCGCCCAAGCACTCACTGCGGCATATCCTCAATCTGTTTCAGGGCCAACCCGGCGCGCGC
CGTTTCCGCCGTCATCTCAGCGAAAACATGCACAAGCCAGGCACAACCCCGCAAGTGCTCATGGACGCCTTGTCGCAACT
GCCTTAG

Upstream 100 bases:

>100_bases
CCGTAATTACAGGCAGCGGCTATTCGGTTGCCGCGCAACAGCGCTGCCACTCTTCCCCCTACGCGCCACCCGCGCAACAT
GCGACAATAGGGACTCTCGT

Downstream 100 bases:

>100_bases
CCTGTCAGTACGCTTTATCCCCGTAGAACAGCATTTAAGCACGAACGGACTGCGAACACTTTTCTAGAACGGTTCCCGGC
TCAAACGCTGCCCGCTACGG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MPPHAANPNHPFDTVLMSNPLSTQLPHQPLDRRLSVAPMMDWTTRDYRFLARLITRHTLLYTEMVVAQAILHGDRDRFLD
FNDEEHPVALQLGGSDPQQLAQATRIAGDYGYDEINLNVGCPSDRVQQGKIGAILMAEPELVARCVEAMQAEVTVPVTVK
TRIGIDDQDDYDFLYRFVDRMEASGCTSLTIHARKAILSGLSPKENRDIPPLIYQRAYAIKHAFPHIEIILNGGVKTLNE
VHEHMGEVDGVMIGREAYQNPYFLAEADRVVFGDDHAIPSRQTIAEQFLPYVERRYALGHAPKHSLRHILNLFQGQPGAR
RFRRHLSENMHKPGTTPQVLMDALSQLP

Sequences:

>Translated_348_residues
MPPHAANPNHPFDTVLMSNPLSTQLPHQPLDRRLSVAPMMDWTTRDYRFLARLITRHTLLYTEMVVAQAILHGDRDRFLD
FNDEEHPVALQLGGSDPQQLAQATRIAGDYGYDEINLNVGCPSDRVQQGKIGAILMAEPELVARCVEAMQAEVTVPVTVK
TRIGIDDQDDYDFLYRFVDRMEASGCTSLTIHARKAILSGLSPKENRDIPPLIYQRAYAIKHAFPHIEIILNGGVKTLNE
VHEHMGEVDGVMIGREAYQNPYFLAEADRVVFGDDHAIPSRQTIAEQFLPYVERRYALGHAPKHSLRHILNLFQGQPGAR
RFRRHLSENMHKPGTTPQVLMDALSQLP
>Mature_347_residues
PPHAANPNHPFDTVLMSNPLSTQLPHQPLDRRLSVAPMMDWTTRDYRFLARLITRHTLLYTEMVVAQAILHGDRDRFLDF
NDEEHPVALQLGGSDPQQLAQATRIAGDYGYDEINLNVGCPSDRVQQGKIGAILMAEPELVARCVEAMQAEVTVPVTVKT
RIGIDDQDDYDFLYRFVDRMEASGCTSLTIHARKAILSGLSPKENRDIPPLIYQRAYAIKHAFPHIEIILNGGVKTLNEV
HEHMGEVDGVMIGREAYQNPYFLAEADRVVFGDDHAIPSRQTIAEQFLPYVERRYALGHAPKHSLRHILNLFQGQPGARR
FRRHLSENMHKPGTTPQVLMDALSQLP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=30.327868852459, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=29.957805907173, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=56.8690095846645, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789660, Length=243, Percent_Identity=27.9835390946502, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788462, Length=202, Percent_Identity=29.2079207920792, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=235, Percent_Identity=27.2340425531915, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=268, Percent_Identity=24.2537313432836, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=28.1512605042017, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=31.7991631799163, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=31.7991631799163, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=27.2340425531915, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549423, Length=322, Percent_Identity=27.0186335403727, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 39306; Mature: 39175

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPHAANPNHPFDTVLMSNPLSTQLPHQPLDRRLSVAPMMDWTTRDYRFLARLITRHTLL
CCCCCCCCCCCCHHHEECCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YTEMVVAQAILHGDRDRFLDFNDEEHPVALQLGGSDPQQLAQATRIAGDYGYDEINLNVG
HHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEC
CPSDRVQQGKIGAILMAEPELVARCVEAMQAEVTVPVTVKTRIGIDDQDDYDFLYRFVDR
CCHHHHCCCCCCEEEEECHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHH
MEASGCTSLTIHARKAILSGLSPKENRDIPPLIYQRAYAIKHAFPHIEIILNGGVKTLNE
HHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
VHEHMGEVDGVMIGREAYQNPYFLAEADRVVFGDDHAIPSRQTIAEQFLPYVERRYALGH
HHHHHHHHCCEEECHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
APKHSLRHILNLFQGQPGARRFRRHLSENMHKPGTTPQVLMDALSQLP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PPHAANPNHPFDTVLMSNPLSTQLPHQPLDRRLSVAPMMDWTTRDYRFLARLITRHTLL
CCCCCCCCCCCHHHEECCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YTEMVVAQAILHGDRDRFLDFNDEEHPVALQLGGSDPQQLAQATRIAGDYGYDEINLNVG
HHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEC
CPSDRVQQGKIGAILMAEPELVARCVEAMQAEVTVPVTVKTRIGIDDQDDYDFLYRFVDR
CCHHHHCCCCCCEEEEECHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHH
MEASGCTSLTIHARKAILSGLSPKENRDIPPLIYQRAYAIKHAFPHIEIILNGGVKTLNE
HHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
VHEHMGEVDGVMIGREAYQNPYFLAEADRVVFGDDHAIPSRQTIAEQFLPYVERRYALGH
HHHHHHHHCCEEECHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
APKHSLRHILNLFQGQPGARRFRRHLSENMHKPGTTPQVLMDALSQLP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]