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The map label for this gene is ispD [H]

Identifier: 110834027

GI number: 110834027

Start: 1327883

End: 1328542

Strand: Direct

Name: ispD [H]

Synonym: ABO_1166

Alternate gene names: 110834027

Gene position: 1327883-1328542 (Clockwise)

Preceding gene: 110834026

Following gene: 110834028

Centisome position: 42.56

GC content: 57.73

Gene sequence:

>660_bases
ATGGGAGCCGGCATTCCCAAGCAGTACCTTTCCCTGACAGGCCAAACCTTGGCAGAACATACTCTCAATCGGTTGTTGTC
TTTTGCACCGATACACAAAGTGGTGGTGGCCGTGTCGGGTGATGATCCCTGGTGGCCGACGCTCTCCGTATCCCGTCATC
CTCGTATCCAGACCGTTAATGGTGGCGACACCCGCGCCGCCTCGGTGCGTAATGGCGTCGCTGCGGTGATAGAGGAAGGC
GGAGAGGATGCCTGGGTGTTGGTGCACGATATGGCTCGCCCGCTGGTTCGCCTCTCCGACATTCAGTTGTTGCTGGATCA
AGCTGGGGATCGAGGCGCGATACTTGCCTTGCCGGTGGTCGATACCATCAAGCAGGCGGATGCGAGTCAGTACATCAAAA
GCACCCTGGATCGTGAGTGTATTTGGCGGGCGCTAACGCCCCAGCTGTTTCCGGCGCGCGGGTTACTGGGGGCGCTTCAA
GGTGACCTTGACGGGATTACTGATGAAGCCTCTGCCATGGAGAAAAATGGCTGGCAGCCGGCACTGGTGGCTGGCCATAG
TGACAATATCAAGGTCACTGTGCCGGACGATTTGCCACTGGCTCGCTTTTATCTTAGCCGGCAACGGGGAGATGACAATG
ATGAAGGAGGACAGTGGTGA

Upstream 100 bases:

>100_bases
CCTTTTTCCTGATCCTAGAACAACCTCGGGGCGCTGAGTGAATCCTGCTATCGCCGGGCCTTTATACGCCGTTGTTCCCG
CCGCAGGGGTGGGTAGCAGA

Downstream 100 bases:

>100_bases
GCGTGCGTATCGGTCAGGGTTTTGATGTTCATGCCTTTGATGAAGGTGACCATGTGATGTTAGGTGGTGTCGCCATCCCC
CACAGCCATGGGTTGAAAGC

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MGAGIPKQYLSLTGQTLAEHTLNRLLSFAPIHKVVVAVSGDDPWWPTLSVSRHPRIQTVNGGDTRAASVRNGVAAVIEEG
GEDAWVLVHDMARPLVRLSDIQLLLDQAGDRGAILALPVVDTIKQADASQYIKSTLDRECIWRALTPQLFPARGLLGALQ
GDLDGITDEASAMEKNGWQPALVAGHSDNIKVTVPDDLPLARFYLSRQRGDDNDEGGQW

Sequences:

>Translated_219_residues
MGAGIPKQYLSLTGQTLAEHTLNRLLSFAPIHKVVVAVSGDDPWWPTLSVSRHPRIQTVNGGDTRAASVRNGVAAVIEEG
GEDAWVLVHDMARPLVRLSDIQLLLDQAGDRGAILALPVVDTIKQADASQYIKSTLDRECIWRALTPQLFPARGLLGALQ
GDLDGITDEASAMEKNGWQPALVAGHSDNIKVTVPDDLPLARFYLSRQRGDDNDEGGQW
>Mature_218_residues
GAGIPKQYLSLTGQTLAEHTLNRLLSFAPIHKVVVAVSGDDPWWPTLSVSRHPRIQTVNGGDTRAASVRNGVAAVIEEGG
EDAWVLVHDMARPLVRLSDIQLLLDQAGDRGAILALPVVDTIKQADASQYIKSTLDRECIWRALTPQLFPARGLLGALQG
DLDGITDEASAMEKNGWQPALVAGHSDNIKVTVPDDLPLARFYLSRQRGDDNDEGGQW

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=219, Percent_Identity=45.2054794520548, Blast_Score=170, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 23732; Mature: 23601

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGIPKQYLSLTGQTLAEHTLNRLLSFAPIHKVVVAVSGDDPWWPTLSVSRHPRIQTVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEC
GGDTRAASVRNGVAAVIEEGGEDAWVLVHDMARPLVRLSDIQLLLDQAGDRGAILALPVV
CCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
DTIKQADASQYIKSTLDRECIWRALTPQLFPARGLLGALQGDLDGITDEASAMEKNGWQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
ALVAGHSDNIKVTVPDDLPLARFYLSRQRGDDNDEGGQW
EEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GAGIPKQYLSLTGQTLAEHTLNRLLSFAPIHKVVVAVSGDDPWWPTLSVSRHPRIQTVN
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEC
GGDTRAASVRNGVAAVIEEGGEDAWVLVHDMARPLVRLSDIQLLLDQAGDRGAILALPVV
CCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
DTIKQADASQYIKSTLDRECIWRALTPQLFPARGLLGALQGDLDGITDEASAMEKNGWQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
ALVAGHSDNIKVTVPDDLPLARFYLSRQRGDDNDEGGQW
EEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA