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The map label for this gene is nasD [H]

Identifier: 110833949

GI number: 110833949

Start: 1232329

End: 1233624

Strand: Direct

Name: nasD [H]

Synonym: ABO_1088

Alternate gene names: 110833949

Gene position: 1232329-1233624 (Clockwise)

Preceding gene: 110833948

Following gene: 110833950

Centisome position: 39.5

GC content: 58.49

Gene sequence:

>1296_bases
ATGAAACGACTGGTTGTAGTGGGTAACGGTATGGCGGCTTGCCGCTTACTGGAAGAGCTGACCCGCCTGGCACCGGATCT
GTATCAGATCACGGTGGTGGGGGATGAGCCCTTTGCCGGCTATAACCGGGTCATGTTGTCCCCCCTGTTGGGTGGTAGCA
CGGATGAAAATACTATTACCACGCATCCTCACCAGTGGTATCGGGAGCGGAGTATTCGCCTGATCACCGGCACCCCCGTC
GTGGCCATTCACCGCGGCCGCCGCCAGGTGGAAACCGCCGCCGGGCACGTGCTCGATTATGACCGGCTATTGCTGGCCAC
AGGGGCGGGGCCACGCCAGTTACCGGTGGACGGCGTTGGTTTGAAAGGCGTTGTCAGTTTTCGTGATCTGTATGACGTTC
GACGCTTGTTATCGCTTTCGGCGGCACGGGTGGTGGTGGTCGGCGGCGGTTTTTTAGGGCTGGAAGCGGCAGACGCTTTG
GTGAAGCAGGGGCACGACGTCACCTTGGTACACAGCCGCGGGCACCTGCTCAACCAGCAGCTGGATGAGGCGGCTGGCCA
TCGCCTGCAGAAGGACCTGGAAGCCCGCGGGATCAAGTTCGTGATGCACGCGCGTACCCAGTCAATTGAAGGTTGTTCTG
TTCAAAAAAAAGAGGATAAAAAAGGCTCAAGCAGCTACAGCTCCGACCGTGTGGCCGCAGTGCATCTGGCGGATGGCCAG
CGTTTGCCGGCGGATTATGTGGTCCAGGCGGTGGGCATTGTGCCGCGGGCAGAGCTGGCCGCTCAGGCTGGCCTGACCGT
AAATCAGGGAGTGCGAGTTGACGATACCCTGCAGACCTTTGATCCGGCCATCTACGCTATTGGTGAGTGTGTGGAGCACC
GTGAACGTACCTTTGGCTTGGTTGCTCCGCTGTATGAACAGGCCTCGGTCTGCGCTAGCCACTTGGCGGAAATGGGTCAT
CGTCGTTATCTCTACGCCGACTCTGCCACCCGGCTGAAAATTTCCGGTATCGACCTGGTGTCCTGCGGGGATTTTCTCGG
CGAGAAAAGGAATGAAATCGAACAGGGTGAGACACTGCAATTGAGCTTGCCTGACAGTTATCGCCGCTTGCAAATTCGCG
ATAACCGTGTGGTCGGCATGGTTCTGTATGGCGATGTGTCTGACGCCCCGTTTTTCGAAACGTTATGGCGTGATAGCACT
GATATTAGCGCCCTGCGCGAAACCCTGTTGCTGGGTGAGGCGTTCTGCAAGCGCCCCGGTGACAAGGGTGATAATGCAGA
GGGAGTGGCGGCGTGA

Upstream 100 bases:

>100_bases
GACGACAGCGTCAAGCTTAAGGTCTATCCGGTGCGCGTGGTCGGTGATGTGATTGAGCTGGCCACCTCGGCCTGAAGCGA
ATCGAGAGGGTGATGCAGTA

Downstream 100 bases:

>100_bases
AGGCGGAAGTGGCAGCCGGCAATGTCGGAGAAAACCGTGCAGGAAAACATATGGCAAAAAACACCGAACGGCCCATTTGT
ACCACCTGCCCGTATTGTGG

Product: nitrite reductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKRLVVVGNGMAACRLLEELTRLAPDLYQITVVGDEPFAGYNRVMLSPLLGGSTDENTITTHPHQWYRERSIRLITGTPV
VAIHRGRRQVETAAGHVLDYDRLLLATGAGPRQLPVDGVGLKGVVSFRDLYDVRRLLSLSAARVVVVGGGFLGLEAADAL
VKQGHDVTLVHSRGHLLNQQLDEAAGHRLQKDLEARGIKFVMHARTQSIEGCSVQKKEDKKGSSSYSSDRVAAVHLADGQ
RLPADYVVQAVGIVPRAELAAQAGLTVNQGVRVDDTLQTFDPAIYAIGECVEHRERTFGLVAPLYEQASVCASHLAEMGH
RRYLYADSATRLKISGIDLVSCGDFLGEKRNEIEQGETLQLSLPDSYRRLQIRDNRVVGMVLYGDVSDAPFFETLWRDST
DISALRETLLLGEAFCKRPGDKGDNAEGVAA

Sequences:

>Translated_431_residues
MKRLVVVGNGMAACRLLEELTRLAPDLYQITVVGDEPFAGYNRVMLSPLLGGSTDENTITTHPHQWYRERSIRLITGTPV
VAIHRGRRQVETAAGHVLDYDRLLLATGAGPRQLPVDGVGLKGVVSFRDLYDVRRLLSLSAARVVVVGGGFLGLEAADAL
VKQGHDVTLVHSRGHLLNQQLDEAAGHRLQKDLEARGIKFVMHARTQSIEGCSVQKKEDKKGSSSYSSDRVAAVHLADGQ
RLPADYVVQAVGIVPRAELAAQAGLTVNQGVRVDDTLQTFDPAIYAIGECVEHRERTFGLVAPLYEQASVCASHLAEMGH
RRYLYADSATRLKISGIDLVSCGDFLGEKRNEIEQGETLQLSLPDSYRRLQIRDNRVVGMVLYGDVSDAPFFETLWRDST
DISALRETLLLGEAFCKRPGDKGDNAEGVAA
>Mature_431_residues
MKRLVVVGNGMAACRLLEELTRLAPDLYQITVVGDEPFAGYNRVMLSPLLGGSTDENTITTHPHQWYRERSIRLITGTPV
VAIHRGRRQVETAAGHVLDYDRLLLATGAGPRQLPVDGVGLKGVVSFRDLYDVRRLLSLSAARVVVVGGGFLGLEAADAL
VKQGHDVTLVHSRGHLLNQQLDEAAGHRLQKDLEARGIKFVMHARTQSIEGCSVQKKEDKKGSSSYSSDRVAAVHLADGQ
RLPADYVVQAVGIVPRAELAAQAGLTVNQGVRVDDTLQTFDPAIYAIGECVEHRERTFGLVAPLYEQASVCASHLAEMGH
RRYLYADSATRLKISGIDLVSCGDFLGEKRNEIEQGETLQLSLPDSYRRLQIRDNRVVGMVLYGDVSDAPFFETLWRDST
DISALRETLLLGEAFCKRPGDKGDNAEGVAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=254, Percent_Identity=29.1338582677165, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI226437568, Length=254, Percent_Identity=29.1338582677165, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI65787454, Length=254, Percent_Identity=29.1338582677165, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4757732, Length=287, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI22202629, Length=287, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789765, Length=422, Percent_Identity=31.9905213270142, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1789065, Length=313, Percent_Identity=28.1150159744409, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788892, Length=292, Percent_Identity=25.6849315068493, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=411, Percent_Identity=24.330900243309, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281359715, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281359713, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639250, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18543267, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639252, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639257, Length=290, Percent_Identity=26.8965517241379, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585130, Length=292, Percent_Identity=25.6849315068493, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 47104; Mature: 47104

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVVVGNGMAACRLLEELTRLAPDLYQITVVGDEPFAGYNRVMLSPLLGGSTDENTIT
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEE
THPHQWYRERSIRLITGTPVVAIHRGRRQVETAAGHVLDYDRLLLATGAGPRQLPVDGVG
CCCHHHHHCCCEEEEECCCEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
LKGVVSFRDLYDVRRLLSLSAARVVVVGGGFLGLEAADALVKQGHDVTLVHSRGHLLNQQ
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECHHHHHHHHHCCCCEEEEECCCHHHHHH
LDEAAGHRLQKDLEARGIKFVMHARTQSIEGCSVQKKEDKKGSSSYSSDRVAAVHLADGQ
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
RLPADYVVQAVGIVPRAELAAQAGLTVNQGVRVDDTLQTFDPAIYAIGECVEHRERTFGL
CCCHHHHHHHHCCCCCHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VAPLYEQASVCASHLAEMGHRRYLYADSATRLKISGIDLVSCGDFLGEKRNEIEQGETLQ
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCEEEECHHHHHHHHHHHCCCCEEE
LSLPDSYRRLQIRDNRVVGMVLYGDVSDAPFFETLWRDSTDISALRETLLLGEAFCKRPG
EECCCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
DKGDNAEGVAA
CCCCCCCCCCC
>Mature Secondary Structure
MKRLVVVGNGMAACRLLEELTRLAPDLYQITVVGDEPFAGYNRVMLSPLLGGSTDENTIT
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEE
THPHQWYRERSIRLITGTPVVAIHRGRRQVETAAGHVLDYDRLLLATGAGPRQLPVDGVG
CCCHHHHHCCCEEEEECCCEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
LKGVVSFRDLYDVRRLLSLSAARVVVVGGGFLGLEAADALVKQGHDVTLVHSRGHLLNQQ
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECHHHHHHHHHCCCCEEEEECCCHHHHHH
LDEAAGHRLQKDLEARGIKFVMHARTQSIEGCSVQKKEDKKGSSSYSSDRVAAVHLADGQ
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
RLPADYVVQAVGIVPRAELAAQAGLTVNQGVRVDDTLQTFDPAIYAIGECVEHRERTFGL
CCCHHHHHHHHCCCCCHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VAPLYEQASVCASHLAEMGHRRYLYADSATRLKISGIDLVSCGDFLGEKRNEIEQGETLQ
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCEEEECHHHHHHHHHHHCCCCEEE
LSLPDSYRRLQIRDNRVVGMVLYGDVSDAPFFETLWRDSTDISALRETLLLGEAFCKRPG
EECCCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
DKGDNAEGVAA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]