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The map label for this gene is nqrF [H]

Identifier: 110833898

GI number: 110833898

Start: 1177632

End: 1178861

Strand: Direct

Name: nqrF [H]

Synonym: ABO_1037

Alternate gene names: 110833898

Gene position: 1177632-1178861 (Clockwise)

Preceding gene: 110833897

Following gene: 110833899

Centisome position: 37.74

GC content: 50.16

Gene sequence:

>1230_bases
ATGAGTTTTGAGATTATTCTCGGTGTTGTCATGTTCACCGCCATCGTGCTCGCGTTGGTGGCAATCATTCTGGTTGCCCG
CTCGCGGCTGGTCAGCACGGGTAATGTGAGCATTGTCATTAACGATGATCCAGATAAATCCATTGAAGCTCCAGCTGGCG
GTAAGCTGTTGGGGACATTGGCAGATCAGGGTATTTTTCTATCTTCTGCCTGTGGCGGTGGTGGTACTTGCGCGCAGTGT
CGGTGTCAGGTGCTGGATGGTGGTGGCGATATTCTTCCCACTGAAGAAGGTCATTTCACCAAAGGTCAGATTCGCGATGG
CTGGCGCTTAAGTTGTCAGGTCAACGTGAAGCAAGACATGGAAATCCAGGTGCCAGAAGAATTCTTCGGCGTTAAGAAGT
GGGAGTGTGAGGTTGTTTCCAATAACAATGTTGCCACTTTCATCAAAGAGCTGACGCTGAAGTTGCCTGAAGGCGAAAAT
GTGGATTTCCGTGCCGGGGGTTATGTACAGCTGGAAGCGCCGCCTTTCGATGTGAAGTTTTCTGACTTCGATATCGATGA
GGAATACCGGGGTGACTGGGAACGGTTCAACTTTTTCGATTTGAACACCAAAAATAACGAAGAAGTGATCCGTGCTTATT
CCATGGCCAACTACCCGGAAGAGAAGGGCATCCTTAAATTCAATATCCGTATTGCGACGCCGCCTCCTGGCTCCAAGGAT
ATTCAACCCGGCATTATGAGCTCATACGTGTTCAGCCTGAAGCCGGGTGACAAGATCACTGTATTTGGTCCATTCGGCGA
GTTCTTCGCTAAGAAAACCGACGCAGAAATGGTGTTCATCGGCGGTGGTGCGGGTATGGCCCCCATGCGTAGCCATATCT
TCGATCAGCTCAAGCGATTGCACTCCAAGCGCAAAATGAGCTTCTGGTATGGTGCCCGCAGTGTTCGCGAATGTTTCTAC
AATGAGGAATATGACAAGCTGGCTGAAGAGAATGACAACTTCGACTGGTACCTAGCTCTGTCAGATCCGCAACCTGAAGA
CAATTGGGATGGCCTCACCGGCTTTATCCACAACGTGCTGTACGAGCAGTATCTTAAGGGCCATCCGGCCCCTGAGGACT
GCGAGTACTACATGTGTGGTCCGCCGATGATGAATGCGTCTGTCATCAAAATGTTGGAAGATTTGGGCGTCGAGCCGGAA
AATATTCTGTTGGATGACTTTGGCGGATGA

Upstream 100 bases:

>100_bases
TGGCCTAAAAGGTCTAGGCATCACCTTCATTACTGTTGGTTTGATGTCACTGGGCTTTATGTCTTTTTCCGGTGTGCAGC
TGTAAGCGAGGGGTTGAATC

Downstream 100 bases:

>100_bases
TTCCAAACGCCGGCCTTCGGGCCGGCGTTTTGTTAGCTACAAGCTACAGTGGAAGCTATGTTTAGGTTTTATCGTTTGTG
ATCGCGCCTTGCCGTTGATC

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSFEIILGVVMFTAIVLALVAIILVARSRLVSTGNVSIVINDDPDKSIEAPAGGKLLGTLADQGIFLSSACGGGGTCAQC
RCQVLDGGGDILPTEEGHFTKGQIRDGWRLSCQVNVKQDMEIQVPEEFFGVKKWECEVVSNNNVATFIKELTLKLPEGEN
VDFRAGGYVQLEAPPFDVKFSDFDIDEEYRGDWERFNFFDLNTKNNEEVIRAYSMANYPEEKGILKFNIRIATPPPGSKD
IQPGIMSSYVFSLKPGDKITVFGPFGEFFAKKTDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSVRECFY
NEEYDKLAEENDNFDWYLALSDPQPEDNWDGLTGFIHNVLYEQYLKGHPAPEDCEYYMCGPPMMNASVIKMLEDLGVEPE
NILLDDFGG

Sequences:

>Translated_409_residues
MSFEIILGVVMFTAIVLALVAIILVARSRLVSTGNVSIVINDDPDKSIEAPAGGKLLGTLADQGIFLSSACGGGGTCAQC
RCQVLDGGGDILPTEEGHFTKGQIRDGWRLSCQVNVKQDMEIQVPEEFFGVKKWECEVVSNNNVATFIKELTLKLPEGEN
VDFRAGGYVQLEAPPFDVKFSDFDIDEEYRGDWERFNFFDLNTKNNEEVIRAYSMANYPEEKGILKFNIRIATPPPGSKD
IQPGIMSSYVFSLKPGDKITVFGPFGEFFAKKTDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSVRECFY
NEEYDKLAEENDNFDWYLALSDPQPEDNWDGLTGFIHNVLYEQYLKGHPAPEDCEYYMCGPPMMNASVIKMLEDLGVEPE
NILLDDFGG
>Mature_408_residues
SFEIILGVVMFTAIVLALVAIILVARSRLVSTGNVSIVINDDPDKSIEAPAGGKLLGTLADQGIFLSSACGGGGTCAQCR
CQVLDGGGDILPTEEGHFTKGQIRDGWRLSCQVNVKQDMEIQVPEEFFGVKKWECEVVSNNNVATFIKELTLKLPEGENV
DFRAGGYVQLEAPPFDVKFSDFDIDEEYRGDWERFNFFDLNTKNNEEVIRAYSMANYPEEKGILKFNIRIATPPPGSKDI
QPGIMSSYVFSLKPGDKITVFGPFGEFFAKKTDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSVRECFYN
EEYDKLAEENDNFDWYLALSDPQPEDNWDGLTGFIHNVLYEQYLKGHPAPEDCEYYMCGPPMMNASVIKMLEDLGVEPEN
ILLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17563928, Length=216, Percent_Identity=26.3888888888889, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323552, Length=195, Percent_Identity=25.1282051282051, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322146, Length=197, Percent_Identity=27.4111675126904, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI116007804, Length=222, Percent_Identity=27.9279279279279, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116007806, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281366101, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24662934, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24662938, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45725; Mature: 45594

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEIILGVVMFTAIVLALVAIILVARSRLVSTGNVSIVINDDPDKSIEAPAGGKLLGTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCEEEEC
ADQGIFLSSACGGGGTCAQCRCQVLDGGGDILPTEEGHFTKGQIRDGWRLSCQVNVKQDM
CCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
EIQVPEEFFGVKKWECEVVSNNNVATFIKELTLKLPEGENVDFRAGGYVQLEAPPFDVKF
EEECCHHHHCCCEEEEEEECCCCHHHHHHHHHEECCCCCCCEEECCCEEEECCCCCCEEE
SDFDIDEEYRGDWERFNFFDLNTKNNEEVIRAYSMANYPEEKGILKFNIRIATPPPGSKD
CCCCCCHHHCCCCHHEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCC
IQPGIMSSYVFSLKPGDKITVFGPFGEFFAKKTDAEMVFIGGGAGMAPMRSHIFDQLKRL
CCCCHHHHEEEEECCCCEEEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
HSKRKMSFWYGARSVRECFYNEEYDKLAEENDNFDWYLALSDPQPEDNWDGLTGFIHNVL
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
YEQYLKGHPAPEDCEYYMCGPPMMNASVIKMLEDLGVEPENILLDDFGG
HHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCC
>Mature Secondary Structure 
SFEIILGVVMFTAIVLALVAIILVARSRLVSTGNVSIVINDDPDKSIEAPAGGKLLGTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCEEEEC
ADQGIFLSSACGGGGTCAQCRCQVLDGGGDILPTEEGHFTKGQIRDGWRLSCQVNVKQDM
CCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
EIQVPEEFFGVKKWECEVVSNNNVATFIKELTLKLPEGENVDFRAGGYVQLEAPPFDVKF
EEECCHHHHCCCEEEEEEECCCCHHHHHHHHHEECCCCCCCEEECCCEEEECCCCCCEEE
SDFDIDEEYRGDWERFNFFDLNTKNNEEVIRAYSMANYPEEKGILKFNIRIATPPPGSKD
CCCCCCHHHCCCCHHEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCC
IQPGIMSSYVFSLKPGDKITVFGPFGEFFAKKTDAEMVFIGGGAGMAPMRSHIFDQLKRL
CCCCHHHHEEEEECCCCEEEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
HSKRKMSFWYGARSVRECFYNEEYDKLAEENDNFDWYLALSDPQPEDNWDGLTGFIHNVL
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
YEQYLKGHPAPEDCEYYMCGPPMMNASVIKMLEDLGVEPENILLDDFGG
HHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA