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The map label for this gene is spoU [H]

Identifier: 110833674

GI number: 110833674

Start: 907327

End: 908019

Strand: Direct

Name: spoU [H]

Synonym: ABO_0813

Alternate gene names: 110833674

Gene position: 907327-908019 (Clockwise)

Preceding gene: 110833672

Following gene: 110833675

Centisome position: 29.08

GC content: 59.31

Gene sequence:

>693_bases
ATGACCCCGGAACGTTACCGTCGCATTTGCGATACCCTGGACCGCCGCCAGCCTGATCTGACTGTCATTATGGACGGTGT
TCACAAACCCCATAATATCGCCGCCATTGTGCGCACCTGCGACGCGGTGGGCATTCTTGATGTACATGCGGTGCTGCCTA
AAAATCGTGCCCGCATGGCTGCAGGGACGGCCATGGGTTCCCAGCGTTGGGTGCAGGTGCACAAACGCGATGAAAGCACC
GACGTGATCCGTGAATTGCAGGGGCAGGGGGTACAGGTGCTGGCGGCACACCTGTCGGATGACGCTATTGAGTATCGGCA
GGTAGATTACACCCGTCCCACTGCACTGCTGTTGGGGACTGAAAAGTTTGGCGTCAGCGATGAGGTGGCCAGCGCGGTGG
ATCAGAATGTAACCATTCCCATGATGGGTATGGTGGAGTCATTTAACGTGAGTGTGGCGGCGGCGATCATTCTTTCCGAG
GCCTGCGAGCAGCGCCGTGCCAAGGGCTTTTATGACCAGCCTCGGGTGGCCGCGGCCCGCTATCATGAGCTGTTGTTCCG
CTGGGGCTATGCCCGTATTGCCCGCTTGTGCGATGCCCACGGCGTGCCGTATCCGGAGCTGGATGAAGAAGGGCAGGTGA
AAGACAGTGCCGCGCTTACGGCGCTGTTGCAGAAGACTCAGACGGAGGCGTAG

Upstream 100 bases:

>100_bases
CGACACCACATAATCGCACTCATAAGCGAGCGGTCTCTTTACTGAATGAAGACGGCCGGCGAAGCGGTGTAGTGATCTGA
ATAGGCTCTAAGGAAAACCC

Downstream 100 bases:

>100_bases
TGGCGGCTTTGTTGGTTGCGATAGACGCCACCGGCCCGAGCATTATTGCCCCCCACGCCGGGAACCGCAGGGTATTGTCC
ATGGTGGCGTAGCGCAGGCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTPERYRRICDTLDRRQPDLTVIMDGVHKPHNIAAIVRTCDAVGILDVHAVLPKNRARMAAGTAMGSQRWVQVHKRDEST
DVIRELQGQGVQVLAAHLSDDAIEYRQVDYTRPTALLLGTEKFGVSDEVASAVDQNVTIPMMGMVESFNVSVAAAIILSE
ACEQRRAKGFYDQPRVAAARYHELLFRWGYARIARLCDAHGVPYPELDEEGQVKDSAALTALLQKTQTEA

Sequences:

>Translated_230_residues
MTPERYRRICDTLDRRQPDLTVIMDGVHKPHNIAAIVRTCDAVGILDVHAVLPKNRARMAAGTAMGSQRWVQVHKRDEST
DVIRELQGQGVQVLAAHLSDDAIEYRQVDYTRPTALLLGTEKFGVSDEVASAVDQNVTIPMMGMVESFNVSVAAAIILSE
ACEQRRAKGFYDQPRVAAARYHELLFRWGYARIARLCDAHGVPYPELDEEGQVKDSAALTALLQKTQTEA
>Mature_229_residues
TPERYRRICDTLDRRQPDLTVIMDGVHKPHNIAAIVRTCDAVGILDVHAVLPKNRARMAAGTAMGSQRWVQVHKRDESTD
VIRELQGQGVQVLAAHLSDDAIEYRQVDYTRPTALLLGTEKFGVSDEVASAVDQNVTIPMMGMVESFNVSVAAAIILSEA
CEQRRAKGFYDQPRVAAARYHELLFRWGYARIARLCDAHGVPYPELDEEGQVKDSAALTALLQKTQTEA

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=226, Percent_Identity=48.6725663716814, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1790623, Length=154, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320091, Length=147, Percent_Identity=27.2108843537415, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25557; Mature: 25426

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPERYRRICDTLDRRQPDLTVIMDGVHKPHNIAAIVRTCDAVGILDVHAVLPKNRARMA
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHH
AGTAMGSQRWVQVHKRDESTDVIRELQGQGVQVLAAHLSDDAIEYRQVDYTRPTALLLGT
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEEC
EKFGVSDEVASAVDQNVTIPMMGMVESFNVSVAAAIILSEACEQRRAKGFYDQPRVAAAR
CCCCCCHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YHELLFRWGYARIARLCDAHGVPYPELDEEGQVKDSAALTALLQKTQTEA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPERYRRICDTLDRRQPDLTVIMDGVHKPHNIAAIVRTCDAVGILDVHAVLPKNRARMA
CHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHH
AGTAMGSQRWVQVHKRDESTDVIRELQGQGVQVLAAHLSDDAIEYRQVDYTRPTALLLGT
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEEC
EKFGVSDEVASAVDQNVTIPMMGMVESFNVSVAAAIILSEACEQRRAKGFYDQPRVAAAR
CCCCCCHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YHELLFRWGYARIARLCDAHGVPYPELDEEGQVKDSAALTALLQKTQTEA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]