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The map label for this gene is trmD

Identifier: 110833661

GI number: 110833661

Start: 893297

End: 894094

Strand: Direct

Name: trmD

Synonym: ABO_0800

Alternate gene names: 110833661

Gene position: 893297-894094 (Clockwise)

Preceding gene: 110833660

Following gene: 110833662

Centisome position: 28.63

GC content: 59.27

Gene sequence:

>798_bases
ATGATGACGACGAAGCCTACGTTTGCCGTCACTCTGGTAACGTTGTTTCCGGAGATGGCACAAGCGATTACTGGCTTTGG
CGTTACTCGGCGGGCTGTCGATAATGGTCAGCTGCAGGTGGAAACGGTGAACCCTCGGGACTTCACCGAAGACCGCCACC
GTACCGTGGATGATCGCCCTTTTGGGGGCGGCCCTGGCATGGTGATGAAAGTGGAGCCGCTGGCGAAAGCCTTACAGGCT
GCACGCGTCGCCAACCCGGCAGCCAAAGTGATTTACCTGTCCCCTCAGGGGCAGCCGTTAACCCAGGCCAAGGCCACGGC
GTTAGCGGAACAGCCTGGCCTAATATTGCTGGCCGGGCGCTATGAAGGCGTAGACGAGCGTTTGCTCGATGCCGAGGTGG
ATGAGCAAATTTCCATCGGCGACTACGTACTCAGCGGTGGTGAATTGCCAGCGCTGGTGCTGATCGATGCGGTTAGCCGA
TTGATACCCGGAGTGTTGGGTCACCAAGACTCCGCCGAGCAGGACTCTTTTTCGGGTGAATTCGAAAACTTGCTCGATTG
CCCGCACTACACCCGCCCGGAGGTGTATGGCGAGCAGGCAGTGCCACCGGTACTGCTCAGCGGCAATCATGAACTGATTC
GCCGCTGGCGCCTTAAGCAGGCGTTGGGACGGACCTGGCAACAGCGTCCAGACCTGCTGGAGGCCCGCCGGGCGCGGGGC
TTGAGCAAAGAAGAGCAACAGCTGCTGGACGAATACATCGCCGAGCAGCCGTCGCATACAGCAAAAACTACTGATTAG

Upstream 100 bases:

>100_bases
TCGGCGTGAGCGTCTGATTCCTTGGCTTCCGGAGGACGTGATCACTGCGATTAGCCTAGAAGACGGCGAAATGACGGTGG
ATTGGGATCCGGACTTTTAA

Downstream 100 bases:

>100_bases
GAGCACTGCCATGAGTGGCAAGAACCTTATTATCCAGGGCATCGAAGAAGCCCAGCTGAAGTCCGAACTTCCGGAATTCA
GTGCCGGTGACACTGTTACC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNPRDFTEDRHRTVDDRPFGGGPGMVMKVEPLAKALQA
ARVANPAAKVIYLSPQGQPLTQAKATALAEQPGLILLAGRYEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSR
LIPGVLGHQDSAEQDSFSGEFENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQALGRTWQQRPDLLEARRARG
LSKEEQQLLDEYIAEQPSHTAKTTD

Sequences:

>Translated_265_residues
MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNPRDFTEDRHRTVDDRPFGGGPGMVMKVEPLAKALQA
ARVANPAAKVIYLSPQGQPLTQAKATALAEQPGLILLAGRYEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSR
LIPGVLGHQDSAEQDSFSGEFENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQALGRTWQQRPDLLEARRARG
LSKEEQQLLDEYIAEQPSHTAKTTD
>Mature_265_residues
MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNPRDFTEDRHRTVDDRPFGGGPGMVMKVEPLAKALQA
ARVANPAAKVIYLSPQGQPLTQAKATALAEQPGLILLAGRYEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSR
LIPGVLGHQDSAEQDSFSGEFENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQALGRTWQQRPDLLEARRARG
LSKEEQQLLDEYIAEQPSHTAKTTD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=254, Percent_Identity=60.2362204724409, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ALCBS (Q0VRF0)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692520.1
- ProteinModelPortal:   Q0VRF0
- SMR:   Q0VRF0
- STRING:   Q0VRF0
- GeneID:   4211924
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0800
- NMPDR:   fig|393595.12.peg.801
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q0VRF0
- BioCyc:   ABOR393595:ABO_0800-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29160; Mature: 29160

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNPRDFTEDRHRTVDDRP
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCCC
FGGGPGMVMKVEPLAKALQAARVANPAAKVIYLSPQGQPLTQAKATALAEQPGLILLAGR
CCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
YEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSRLIPGVLGHQDSAEQDSFSGE
CCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQALGRTWQQRPDLLEARRARG
HHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
LSKEEQQLLDEYIAEQPSHTAKTTD
CCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNPRDFTEDRHRTVDDRP
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCCC
FGGGPGMVMKVEPLAKALQAARVANPAAKVIYLSPQGQPLTQAKATALAEQPGLILLAGR
CCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
YEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSRLIPGVLGHQDSAEQDSFSGE
CCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQALGRTWQQRPDLLEARRARG
HHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
LSKEEQQLLDEYIAEQPSHTAKTTD
CCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA