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The map label for this gene is ddl

Identifier: 110833461

GI number: 110833461

Start: 658464

End: 659387

Strand: Direct

Name: ddl

Synonym: ABO_0600

Alternate gene names: 110833461

Gene position: 658464-659387 (Clockwise)

Preceding gene: 110833460

Following gene: 110833462

Centisome position: 21.1

GC content: 58.66

Gene sequence:

>924_bases
ATGGATAAGCGTGCATTGAAAGAACGATTGAGCCGAGTAGGCCGCGTGGCAGTGCTGGCTGGTGGTCGATCTGCAGAACG
CGCTGTGTCGCTGAAGAGCGGTGCTGCGGTACATGCAGGCTTGCGTAACTTGGGTTTGCTCGCAGAGCTGGTGGACCCGG
CGGATAAGAGCGTCGATACCTTGAGAGGGTTTGATGCCGCCTTTATTGCCTTGCATGGCCGCGGTGGCGAAGACGGCGTG
ATTCAAGGCGTATTGGAGCACCTTGAGATTCCCTACACAGGCTCCGGCGTGATGGCGTCGGCTATCGGCATGGACAAGGT
ACGTACCAAGCAGCTGTGGAAAGGTGCGGGTTTGCCCACGCCAGCCTTTTATGTGGCTGGGCGTGAAGAGGCAGAGTTGG
GCTTTCCGGTGATCGTCAAGCCAGCCCATGAAGGGTCGTCCATCGGCATGGCCAAGGCCGATAACACCGAGGAACTGGGC
GAAGCGTTAGTGGCCGCGGAAAAATTTGATCAGGACGTGTTGGTTGAAGCCTGGGTCAACGGCCCGGAGTACACCGTGGC
GGTGCTGGGTGATGAAGCTTTGCCGGCTATCCGCCTGCAAACGCCCCATGTCTTCTATGACTTTGAAGCCAAGTACCAGT
CCGACAGCACGGAGTATTTATGTCCGGCTGGGCTGGATGATGCTGATGAGCAGGCGTTGCGCACGTTGGCGCTCACGGCA
TTCCGCGTTGCCGGATGCCGTGGCTGGGGCCGGGTAGATGTCATGCGTGATGAGCAAGGCCAGTGGCAGTTGCTGGAAGT
GAATACGGTGCCAGGTATGACCGACCATTCTTTAGTGCCGATGGCTGCGAAAGCCACTGGCCGTGATTTCGATGTGCTGG
TAGGTGAGATTCTGCTGGACGCGCTGGAGCGGGGTCGTGGCTAA

Upstream 100 bases:

>100_bases
TTGTTGGCCAGCCAGCTTCAGAATGGCGATCTGCTCGTCACCCAAGGCGCGGGTAATGTCGGTGCCATCGCCAAGCAGTT
GGTTGCTCAGGGAGGCGGCC

Downstream 100 bases:

>100_bases
AAACGCGCCCGCGCCGCGTGGCGCCCGCCGCAAGCCGGTTAAAAAGGTCGGCGTACCGCTGCGCGAGCGGGTGGCAACGG
CGGTGCCATGGATGCTGGTC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MDKRALKERLSRVGRVAVLAGGRSAERAVSLKSGAAVHAGLRNLGLLAELVDPADKSVDTLRGFDAAFIALHGRGGEDGV
IQGVLEHLEIPYTGSGVMASAIGMDKVRTKQLWKGAGLPTPAFYVAGREEAELGFPVIVKPAHEGSSIGMAKADNTEELG
EALVAAEKFDQDVLVEAWVNGPEYTVAVLGDEALPAIRLQTPHVFYDFEAKYQSDSTEYLCPAGLDDADEQALRTLALTA
FRVAGCRGWGRVDVMRDEQGQWQLLEVNTVPGMTDHSLVPMAAKATGRDFDVLVGEILLDALERGRG

Sequences:

>Translated_307_residues
MDKRALKERLSRVGRVAVLAGGRSAERAVSLKSGAAVHAGLRNLGLLAELVDPADKSVDTLRGFDAAFIALHGRGGEDGV
IQGVLEHLEIPYTGSGVMASAIGMDKVRTKQLWKGAGLPTPAFYVAGREEAELGFPVIVKPAHEGSSIGMAKADNTEELG
EALVAAEKFDQDVLVEAWVNGPEYTVAVLGDEALPAIRLQTPHVFYDFEAKYQSDSTEYLCPAGLDDADEQALRTLALTA
FRVAGCRGWGRVDVMRDEQGQWQLLEVNTVPGMTDHSLVPMAAKATGRDFDVLVGEILLDALERGRG
>Mature_307_residues
MDKRALKERLSRVGRVAVLAGGRSAERAVSLKSGAAVHAGLRNLGLLAELVDPADKSVDTLRGFDAAFIALHGRGGEDGV
IQGVLEHLEIPYTGSGVMASAIGMDKVRTKQLWKGAGLPTPAFYVAGREEAELGFPVIVKPAHEGSSIGMAKADNTEELG
EALVAAEKFDQDVLVEAWVNGPEYTVAVLGDEALPAIRLQTPHVFYDFEAKYQSDSTEYLCPAGLDDADEQALRTLALTA
FRVAGCRGWGRVDVMRDEQGQWQLLEVNTVPGMTDHSLVPMAAKATGRDFDVLVGEILLDALERGRG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=299, Percent_Identity=55.5183946488294, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1786579, Length=260, Percent_Identity=35.3846153846154, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ALCBS (Q0VS00)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692320.1
- ProteinModelPortal:   Q0VS00
- SMR:   Q0VS00
- STRING:   Q0VS00
- GeneID:   4212209
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0600
- NMPDR:   fig|393595.12.peg.598
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- PhylomeDB:   Q0VS00
- ProtClustDB:   PRK01372
- BioCyc:   ABOR393595:ABO_0600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32852; Mature: 32852

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRALKERLSRVGRVAVLAGGRSAERAVSLKSGAAVHAGLRNLGLLAELVDPADKSVDT
CCHHHHHHHHHHCCEEEEEECCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LRGFDAAFIALHGRGGEDGVIQGVLEHLEIPYTGSGVMASAIGMDKVRTKQLWKGAGLPT
HHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCC
PAFYVAGREEAELGFPVIVKPAHEGSSIGMAKADNTEELGEALVAAEKFDQDVLVEAWVN
CEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEHEECC
GPEYTVAVLGDEALPAIRLQTPHVFYDFEAKYQSDSTEYLCPAGLDDADEQALRTLALTA
CCCEEEEEECCCCCCEEEECCCEEEEEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHH
FRVAGCRGWGRVDVMRDEQGQWQLLEVNTVPGMTDHSLVPMAAKATGRDFDVLVGEILLD
HHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEHHCCCCHHHHHHHHHHHH
ALERGRG
HHHHCCC
>Mature Secondary Structure
MDKRALKERLSRVGRVAVLAGGRSAERAVSLKSGAAVHAGLRNLGLLAELVDPADKSVDT
CCHHHHHHHHHHCCEEEEEECCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LRGFDAAFIALHGRGGEDGVIQGVLEHLEIPYTGSGVMASAIGMDKVRTKQLWKGAGLPT
HHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCC
PAFYVAGREEAELGFPVIVKPAHEGSSIGMAKADNTEELGEALVAAEKFDQDVLVEAWVN
CEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEHEECC
GPEYTVAVLGDEALPAIRLQTPHVFYDFEAKYQSDSTEYLCPAGLDDADEQALRTLALTA
CCCEEEEEECCCCCCEEEECCCEEEEEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHH
FRVAGCRGWGRVDVMRDEQGQWQLLEVNTVPGMTDHSLVPMAAKATGRDFDVLVGEILLD
HHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEHHCCCCHHHHHHHHHHHH
ALERGRG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA