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The map label for this gene is murD

Identifier: 110833457

GI number: 110833457

Start: 653512

End: 654837

Strand: Direct

Name: murD

Synonym: ABO_0596

Alternate gene names: 110833457

Gene position: 653512-654837 (Clockwise)

Preceding gene: 110833456

Following gene: 110833458

Centisome position: 20.94

GC content: 59.2

Gene sequence:

>1326_bases
ATGGCGAAAGACGGGTTTGATCTGGTCATTGGCCTGGGTGTAACAGGCCGCTCCGTGATCCGCTATCTAACGGATCAGGG
TATGCCCGTGCGCGCCATAGATACCCGTGCTGAACCGGTCGGCCTAGACGCCCTGTGCGCAGACTTTCCGGACCTAAAAG
TGCATACCGGTGGCTTCAAGAAAGGCTGGATGGGCAAGGCCCGTCGTCTGATCGTGAGCCCGGGTGTGGCGGTGTCCACG
CCCGCCATTGCCGAGCAGGTAGTGGATGGCAAAGAAGTGATCGGCGACATTGAGCTGTTCGCCCGTGCGGCCAGTGAGCC
GCTGGTGGCCATCACCGGTTCCAATGCCAAAAGCACAGTGACAACGCTGCTGGGGCAAGTGGCAGATGCCTGTGGGATGA
ACCCAGGTATCGGCGGCAACCTGGGTGTGCCAGCGCTGGAATTGCTGGACGACCAAGCGCGGTTGTACGTTCTTGAGTTG
TCCAGCTTTCAGTTGGAAACCACTTACAGCTTGAGTGCGGAAGTGGCCACAGTGTTAAACGTGTCCCAGGATCATTTGGA
CCGTTACGCCAGCTTTGCAGATTACCTAGCGGCTAAGCAGCGCGTCTATGACGGCTGCCAGGTGGCGGTCTGGAATCGTG
ATGATTTGGCAACCCGTCCGCCGGCTACGGTGGCCCGGCAGATCAGTTTTGGCGCCCACCCGGAAGCGGATTACCGGCTA
GATAGCGAAAACGGTCAACTACTGTGCCGAGGCGAGCCCTTATTGTCGCTGTCAGAGCTGGCGCTCACCGGCCACCATAA
TGCCATGAATATTCTCTCGGTTCTGGCGATCAGTGATGCGCTGTCTTTGAATCGGGATAAAGCACTGGCCACAGTGAAAA
CCTTTACTGGGCTGCCGCATCGTTGCCAGCTGGTCGCCGAATCAGGTGGTGTGCGCTGGTTTAACGATTCGAAGGCCACC
AATGTGGGCGCCACCTTGGCGGCGCTGACCGGGATCGGTGAAAGCATCGAGGGCAAAGTGATTCTGGTCGCTGGTGGCCT
AGGCAAAGGGCAGGATTTCTCTCCGCTGGCCGAGCCTGCCCGGCAGTATTTACGTGCCGCCCTATTGATGGGTGAAGACC
GTACTACCGTGGCACAGGGCATGTCTGCGGCGCCCTGTGAACTGGTGGCAGATATGGCTGTTGCGGTACGTCGCGCCCAT
GCCCTGGCACAGCCGGGCGATGCCGTTTTACTGTCACCGGCCTGTGCCTCGTTTGATCAGTACAGCGGTTTTGCTGCCCG
CGGCGATGATTTCACCACCCGTGCCCAGGAGTTGTGCCATGACTGA

Upstream 100 bases:

>100_bases
CCGGAGCCGAAGATTATTGTGCGGTTCTGGATCATCACCGTGATTCTTGTACTTGTGGGTCTGGCGACCCTGAAAATCCG
ATAGGGGATTGAGTCAATTT

Downstream 100 bases:

>100_bases
GCGCATGATCCTGAAAGTAGACCATACGGGGATCGACAAACCGCTGTTGTGGACGGCTATTTTGTTGGCTCTGGCAGGGC
TAGTGATGGTGTCATCCGCC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAKDGFDLVIGLGVTGRSVIRYLTDQGMPVRAIDTRAEPVGLDALCADFPDLKVHTGGFKKGWMGKARRLIVSPGVAVST
PAIAEQVVDGKEVIGDIELFARAASEPLVAITGSNAKSTVTTLLGQVADACGMNPGIGGNLGVPALELLDDQARLYVLEL
SSFQLETTYSLSAEVATVLNVSQDHLDRYASFADYLAAKQRVYDGCQVAVWNRDDLATRPPATVARQISFGAHPEADYRL
DSENGQLLCRGEPLLSLSELALTGHHNAMNILSVLAISDALSLNRDKALATVKTFTGLPHRCQLVAESGGVRWFNDSKAT
NVGATLAALTGIGESIEGKVILVAGGLGKGQDFSPLAEPARQYLRAALLMGEDRTTVAQGMSAAPCELVADMAVAVRRAH
ALAQPGDAVLLSPACASFDQYSGFAARGDDFTTRAQELCHD

Sequences:

>Translated_441_residues
MAKDGFDLVIGLGVTGRSVIRYLTDQGMPVRAIDTRAEPVGLDALCADFPDLKVHTGGFKKGWMGKARRLIVSPGVAVST
PAIAEQVVDGKEVIGDIELFARAASEPLVAITGSNAKSTVTTLLGQVADACGMNPGIGGNLGVPALELLDDQARLYVLEL
SSFQLETTYSLSAEVATVLNVSQDHLDRYASFADYLAAKQRVYDGCQVAVWNRDDLATRPPATVARQISFGAHPEADYRL
DSENGQLLCRGEPLLSLSELALTGHHNAMNILSVLAISDALSLNRDKALATVKTFTGLPHRCQLVAESGGVRWFNDSKAT
NVGATLAALTGIGESIEGKVILVAGGLGKGQDFSPLAEPARQYLRAALLMGEDRTTVAQGMSAAPCELVADMAVAVRRAH
ALAQPGDAVLLSPACASFDQYSGFAARGDDFTTRAQELCHD
>Mature_440_residues
AKDGFDLVIGLGVTGRSVIRYLTDQGMPVRAIDTRAEPVGLDALCADFPDLKVHTGGFKKGWMGKARRLIVSPGVAVSTP
AIAEQVVDGKEVIGDIELFARAASEPLVAITGSNAKSTVTTLLGQVADACGMNPGIGGNLGVPALELLDDQARLYVLELS
SFQLETTYSLSAEVATVLNVSQDHLDRYASFADYLAAKQRVYDGCQVAVWNRDDLATRPPATVARQISFGAHPEADYRLD
SENGQLLCRGEPLLSLSELALTGHHNAMNILSVLAISDALSLNRDKALATVKTFTGLPHRCQLVAESGGVRWFNDSKATN
VGATLAALTGIGESIEGKVILVAGGLGKGQDFSPLAEPARQYLRAALLMGEDRTTVAQGMSAAPCELVADMAVAVRRAHA
LAQPGDAVLLSPACASFDQYSGFAARGDDFTTRAQELCHD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=440, Percent_Identity=47.0454545454545, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_ALCBS (Q0VS04)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692316.1
- ProteinModelPortal:   Q0VS04
- SMR:   Q0VS04
- STRING:   Q0VS04
- GeneID:   4211242
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0596
- NMPDR:   fig|393595.12.peg.594
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q0VS04
- BioCyc:   ABOR393595:ABO_0596-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46537; Mature: 46406

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDGFDLVIGLGVTGRSVIRYLTDQGMPVRAIDTRAEPVGLDALCADFPDLKVHTGGFK
CCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCCCCEEECCCCC
KGWMGKARRLIVSPGVAVSTPAIAEQVVDGKEVIGDIELFARAASEPLVAITGSNAKSTV
CCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TTLLGQVADACGMNPGIGGNLGVPALELLDDQARLYVLELSSFQLETTYSLSAEVATVLN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCEEEEEEEECHHHHHHHHC
VSQDHLDRYASFADYLAAKQRVYDGCQVAVWNRDDLATRPPATVARQISFGAHPEADYRL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEE
DSENGQLLCRGEPLLSLSELALTGHHNAMNILSVLAISDALSLNRDKALATVKTFTGLPH
ECCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RCQLVAESGGVRWFNDSKATNVGATLAALTGIGESIEGKVILVAGGLGKGQDFSPLAEPA
HEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
RQYLRAALLMGEDRTTVAQGMSAAPCELVADMAVAVRRAHALAQPGDAVLLSPACASFDQ
HHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCHHH
YSGFAARGDDFTTRAQELCHD
CCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKDGFDLVIGLGVTGRSVIRYLTDQGMPVRAIDTRAEPVGLDALCADFPDLKVHTGGFK
CCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCCCCEEECCCCC
KGWMGKARRLIVSPGVAVSTPAIAEQVVDGKEVIGDIELFARAASEPLVAITGSNAKSTV
CCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TTLLGQVADACGMNPGIGGNLGVPALELLDDQARLYVLELSSFQLETTYSLSAEVATVLN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCEEEEEEEECHHHHHHHHC
VSQDHLDRYASFADYLAAKQRVYDGCQVAVWNRDDLATRPPATVARQISFGAHPEADYRL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEE
DSENGQLLCRGEPLLSLSELALTGHHNAMNILSVLAISDALSLNRDKALATVKTFTGLPH
ECCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RCQLVAESGGVRWFNDSKATNVGATLAALTGIGESIEGKVILVAGGLGKGQDFSPLAEPA
HEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
RQYLRAALLMGEDRTTVAQGMSAAPCELVADMAVAVRRAHALAQPGDAVLLSPACASFDQ
HHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCHHH
YSGFAARGDDFTTRAQELCHD
CCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA