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The map label for this gene is trmJ [H]

Identifier: 110833374

GI number: 110833374

Start: 564704

End: 565489

Strand: Direct

Name: trmJ [H]

Synonym: ABO_0513

Alternate gene names: 110833374

Gene position: 564704-565489 (Clockwise)

Preceding gene: 110833366

Following gene: 110833375

Centisome position: 18.1

GC content: 58.78

Gene sequence:

>786_bases
ATGTTGGAAAATGTGCGCATCGTGATGGTGGAAACCTCCCACCCCGGCAATATCGGCGCGGCTGCCCGGGCTATGAAAAC
CATGGGCCTGTCGGACTTGCGGTTGGTGCAGCCACAGTCCTATCCCAGCGTGGAGGCGACCGCCCGTGCCTCCGGTGCCG
GGGACGTGCTGGAGGCGGCGCAAGTGTGCAACAGCGTGGATGAGGCGCTGGAAGGCGCCACTTTGGTGATCGGTACTAGC
GCGCGCCAACGCCGTATTCCCTGGCCCTGCCTGACGCCGCGCCAGTTGGCTGCACAGCTCTCCCACGATTTGGCCAATGC
GCCGCAGGATACCCGCATTGCGGTGTTGTTCGGTCGTGAGGACCGGGGGCTGACCAACGACGAGCTGCGCCGCTGCAATC
TCCACGTGAGTGTGCCCGCTAATCCAGAGTATGGGGTCTTGAATGTGGCGTCGGCGGTTCAATTAATCAGTTACGAGTTG
CGTCTTGCGTTGTTGGGGGATGATTTCGAGTTGCAGGAATCTCGCTCCCGCCGTGAAGTGATGCCACTGCCAGAAATGTT
CTGGGATGAACCGCCGGCCACCAATGAAGCGGTCTCTGGTTTTCTCGATCATTTGGAGAAGGTGATGGCACAGAGTGGTT
TTTTAAATCCGCAACAGCCAGGTCAAGTAATGACACGCATGCGTCGTTTGTTCATGCGCGCTCGCCCGGACAAAATGGAA
ATGAGCATTTTGCGCGGCGTATTGGTGGCGCTGGAGAAAAAAATGCAGCGTGATGAGGGCCGATAG

Upstream 100 bases:

>100_bases
ACTCAAGGTTCCCGTTCCGGGGCGCGGATTGTAGCAGAGCCCGCGCGATCAGGGCAGCCGCCGTGTTAAGATTAGCGCCT
TTTTTAATGAGAGGCCTCCC

Downstream 100 bases:

>100_bases
GCCATAGCAAACGGCCACTGGTGTCATCCTAATGGGCCTTGGTCCTAGGTTTCAAAATGAGGTAGTTATGTTGAATCAAG
TGCGCGAGGACATTCGTTCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLENVRIVMVETSHPGNIGAAARAMKTMGLSDLRLVQPQSYPSVEATARASGAGDVLEAAQVCNSVDEALEGATLVIGTS
ARQRRIPWPCLTPRQLAAQLSHDLANAPQDTRIAVLFGREDRGLTNDELRRCNLHVSVPANPEYGVLNVASAVQLISYEL
RLALLGDDFELQESRSRREVMPLPEMFWDEPPATNEAVSGFLDHLEKVMAQSGFLNPQQPGQVMTRMRRLFMRARPDKME
MSILRGVLVALEKKMQRDEGR

Sequences:

>Translated_261_residues
MLENVRIVMVETSHPGNIGAAARAMKTMGLSDLRLVQPQSYPSVEATARASGAGDVLEAAQVCNSVDEALEGATLVIGTS
ARQRRIPWPCLTPRQLAAQLSHDLANAPQDTRIAVLFGREDRGLTNDELRRCNLHVSVPANPEYGVLNVASAVQLISYEL
RLALLGDDFELQESRSRREVMPLPEMFWDEPPATNEAVSGFLDHLEKVMAQSGFLNPQQPGQVMTRMRRLFMRARPDKME
MSILRGVLVALEKKMQRDEGR
>Mature_261_residues
MLENVRIVMVETSHPGNIGAAARAMKTMGLSDLRLVQPQSYPSVEATARASGAGDVLEAAQVCNSVDEALEGATLVIGTS
ARQRRIPWPCLTPRQLAAQLSHDLANAPQDTRIAVLFGREDRGLTNDELRRCNLHVSVPANPEYGVLNVASAVQLISYEL
RLALLGDDFELQESRSRREVMPLPEMFWDEPPATNEAVSGFLDHLEKVMAQSGFLNPQQPGQVMTRMRRLFMRARPDKME
MSILRGVLVALEKKMQRDEGR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=254, Percent_Identity=47.244094488189, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=36.5384615384615, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28945; Mature: 28945

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENVRIVMVETSHPGNIGAAARAMKTMGLSDLRLVQPQSYPSVEATARASGAGDVLEAA
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHCCCCCHHHHHHH
QVCNSVDEALEGATLVIGTSARQRRIPWPCLTPRQLAAQLSHDLANAPQDTRIAVLFGRE
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
DRGLTNDELRRCNLHVSVPANPEYGVLNVASAVQLISYELRLALLGDDFELQESRSRREV
CCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCC
MPLPEMFWDEPPATNEAVSGFLDHLEKVMAQSGFLNPQQPGQVMTRMRRLFMRARPDKME
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
MSILRGVLVALEKKMQRDEGR
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLENVRIVMVETSHPGNIGAAARAMKTMGLSDLRLVQPQSYPSVEATARASGAGDVLEAA
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHCCCCCHHHHHHH
QVCNSVDEALEGATLVIGTSARQRRIPWPCLTPRQLAAQLSHDLANAPQDTRIAVLFGRE
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
DRGLTNDELRRCNLHVSVPANPEYGVLNVASAVQLISYELRLALLGDDFELQESRSRREV
CCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCC
MPLPEMFWDEPPATNEAVSGFLDHLEKVMAQSGFLNPQQPGQVMTRMRRLFMRARPDKME
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
MSILRGVLVALEKKMQRDEGR
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA