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The map label for this gene is ilvB-1 [H]

Identifier: 110833342

GI number: 110833342

Start: 523056

End: 524777

Strand: Direct

Name: ilvB-1 [H]

Synonym: ABO_0481

Alternate gene names: 110833342

Gene position: 523056-524777 (Clockwise)

Preceding gene: 110833340

Following gene: 110833343

Centisome position: 16.76

GC content: 57.43

Gene sequence:

>1722_bases
GTGGAAATGTTATCCGGCGCGGAAATGGTGGTCCGTGCGCTGCAAGACGAGGGAGTGGAGTACATCTTCGGGTATCCCGG
AGGGTCCGTACTGCACATTTATGATGCGCTGTTCCAGCAGGACAAGGTCAACCATATTCTGGTCCGCCATGAGCAGGCGG
CCACCCATGCGGCCGACGGCTATGCCCGTGCCACCGGCAAGGCCGGTGTGGTCATGGTGACGTCCGGGCCAGGCGCGACT
AATGCGATCACTGGCTTGGCTACGGCCTTTATGGATTCGATTCCTATGGTAGTGCTGTCCGGGCAAGTGCGTTCTGACTG
GATTGGTGATGATGCCTTCCAGGAAACGGACATGATTGGTGTGTCCCGTCCGGTGGTGAAGCACAGTTTCATGGTTCGTG
ATGCCAAAGACATTCCCGAAATCATGAAGAAGGCCTTCTACATCGCCACCACCGGCCGTCCCGGCCCGGTGCTGGTGGAT
ATTCCTAAGGATCAGACGGCACCGAACGTCAAGAATCCCTACGAGTATCCCAAGAAAGTGAAGATGCGCTCTTACTCTCC
GGTGAGCCGCGGCCATTCCGGGCAGATCCGTAAGGCGCTGGACGAGTTGATGAAAGCCAAGCGCCCGGTGATTTATGCCG
GCGGTGGCGTGGTGCAGGGTGAAGGCAGCGAGTTGCTGACCCAGTTGGCCCACCGGCTTAATTTCCCGGTGACCAATACC
CTGATGGGACTGGGGGGCTTTCCCGGTACCGACCGTCAGAACGTGGGTATGCTGGGCATGCACGGCACCTATGAAGCCAA
CATGACCATGCATAACTCGGATGTGATTTTGGCTGTGGGTGCCCGTCTGGACGACCGGGTGACTAACAATGTGAAGAAGT
TCTGCCCGGGCGCCAAGATCATTCACATCGATATCGACCCGGCGACTATCTCTAAAAACATCATGGCGCACATCCCCATC
GTGGGCCCGGTGCAGCAGGTGCTGGAAGAGATGCTGGCGGCGCTGGATGAGCACGGTGGCGAGCCGGACCGCAAGGCGCT
TGAGCGCTGGTGGGAAGAGATCGACGGCTGGCGCAAGGTGCATGGCATGCGTTATGAGATCAACGAAGATGGTCCCATGA
AGCCCCAGCAGGTGGTGCAGGCGCTATATAAGGCAACCAAAGGTGATGCCTATGTGACTTCCGATGTGGGTCAGCACCAG
ATGTTCGCAGCCCAGTACTATCAGTACGACAAGCCTCGCCGCTGGATCAACTCCGGTGGCCTGGGGACTATGGGCTTCGG
CTTGCCGGCGGCCATGGGGGTGCAGTTTGCGTTCCCCGAGGCGACGGTGGCCTGTGTGACGGGCGAAGGCTCTATTCAGA
TGAATATCCAAGAACTGTCCACCTGCCTGCAGTATGGCTTGCCGGTGAAAATCATCAACATTAATAACCAGGCGCTGGGC
ATGGTTCGCCAATGGCAGGACATGCAGTACGGCGGCCGTCACAGCCAGTCATACATGGAGTCCCTGCCGGACTTCGTCAA
GCTGGCCGAATCTTATGGCCATGTGGGCATGAAGATCACCAAGATCGAGGATCTGGACGCGGCTATGGACGAAGCCTTTG
CCCTGAAAGACCGTTTGGTATTCATGGATATTTATGTTGATTCCACCGAGCACGTATACCCTATGCAGATTGCAGACGGT
TCCATGCGTGATATGTGGTTGAGCAAGACGGAGCGGACCTGA

Upstream 100 bases:

>100_bases
CGGTGCTTTCCGCAGGCAGCAAGCTAGCAGGCTGTTCAACAGCCACACCGTCCCGCTGCGTGGCAGTGGGTAGTATGCAT
TTAAATTTGAGGTAGCAACC

Downstream 100 bases:

>100_bases
TGCGTCGAATTATTTCTATTCTGATGGAAAACGAGCCGGGCGCACTGAGCCGGGTAATCGGTCTGTTCTCCCAGCGGGGG
TACAACATTGAAACCCTTTC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEMLSGAEMVVRALQDEGVEYIFGYPGGSVLHIYDALFQQDKVNHILVRHEQAATHAADGYARATGKAGVVMVTSGPGAT
NAITGLATAFMDSIPMVVLSGQVRSDWIGDDAFQETDMIGVSRPVVKHSFMVRDAKDIPEIMKKAFYIATTGRPGPVLVD
IPKDQTAPNVKNPYEYPKKVKMRSYSPVSRGHSGQIRKALDELMKAKRPVIYAGGGVVQGEGSELLTQLAHRLNFPVTNT
LMGLGGFPGTDRQNVGMLGMHGTYEANMTMHNSDVILAVGARLDDRVTNNVKKFCPGAKIIHIDIDPATISKNIMAHIPI
VGPVQQVLEEMLAALDEHGGEPDRKALERWWEEIDGWRKVHGMRYEINEDGPMKPQQVVQALYKATKGDAYVTSDVGQHQ
MFAAQYYQYDKPRRWINSGGLGTMGFGLPAAMGVQFAFPEATVACVTGEGSIQMNIQELSTCLQYGLPVKIININNQALG
MVRQWQDMQYGGRHSQSYMESLPDFVKLAESYGHVGMKITKIEDLDAAMDEAFALKDRLVFMDIYVDSTEHVYPMQIADG
SMRDMWLSKTERT

Sequences:

>Translated_573_residues
MEMLSGAEMVVRALQDEGVEYIFGYPGGSVLHIYDALFQQDKVNHILVRHEQAATHAADGYARATGKAGVVMVTSGPGAT
NAITGLATAFMDSIPMVVLSGQVRSDWIGDDAFQETDMIGVSRPVVKHSFMVRDAKDIPEIMKKAFYIATTGRPGPVLVD
IPKDQTAPNVKNPYEYPKKVKMRSYSPVSRGHSGQIRKALDELMKAKRPVIYAGGGVVQGEGSELLTQLAHRLNFPVTNT
LMGLGGFPGTDRQNVGMLGMHGTYEANMTMHNSDVILAVGARLDDRVTNNVKKFCPGAKIIHIDIDPATISKNIMAHIPI
VGPVQQVLEEMLAALDEHGGEPDRKALERWWEEIDGWRKVHGMRYEINEDGPMKPQQVVQALYKATKGDAYVTSDVGQHQ
MFAAQYYQYDKPRRWINSGGLGTMGFGLPAAMGVQFAFPEATVACVTGEGSIQMNIQELSTCLQYGLPVKIININNQALG
MVRQWQDMQYGGRHSQSYMESLPDFVKLAESYGHVGMKITKIEDLDAAMDEAFALKDRLVFMDIYVDSTEHVYPMQIADG
SMRDMWLSKTERT
>Mature_573_residues
MEMLSGAEMVVRALQDEGVEYIFGYPGGSVLHIYDALFQQDKVNHILVRHEQAATHAADGYARATGKAGVVMVTSGPGAT
NAITGLATAFMDSIPMVVLSGQVRSDWIGDDAFQETDMIGVSRPVVKHSFMVRDAKDIPEIMKKAFYIATTGRPGPVLVD
IPKDQTAPNVKNPYEYPKKVKMRSYSPVSRGHSGQIRKALDELMKAKRPVIYAGGGVVQGEGSELLTQLAHRLNFPVTNT
LMGLGGFPGTDRQNVGMLGMHGTYEANMTMHNSDVILAVGARLDDRVTNNVKKFCPGAKIIHIDIDPATISKNIMAHIPI
VGPVQQVLEEMLAALDEHGGEPDRKALERWWEEIDGWRKVHGMRYEINEDGPMKPQQVVQALYKATKGDAYVTSDVGQHQ
MFAAQYYQYDKPRRWINSGGLGTMGFGLPAAMGVQFAFPEATVACVTGEGSIQMNIQELSTCLQYGLPVKIININNQALG
MVRQWQDMQYGGRHSQSYMESLPDFVKLAESYGHVGMKITKIEDLDAAMDEAFALKDRLVFMDIYVDSTEHVYPMQIADG
SMRDMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=25.886524822695, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI21361361, Length=559, Percent_Identity=25.0447227191413, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=63.1304347826087, Blast_Score=782, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=39.5306859205776, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1786717, Length=547, Percent_Identity=34.3692870201097, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=28.1081081081081, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=25.3703703703704, Blast_Score=158, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=580, Percent_Identity=25.1724137931034, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=580, Percent_Identity=25.1724137931034, Blast_Score=166, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=582, Percent_Identity=24.914089347079, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=43.0034129692833, Blast_Score=455, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=490, Percent_Identity=22.6530612244898, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922626, Length=600, Percent_Identity=25.6666666666667, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63287; Mature: 63287

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRALQDEGVEYIFGYPGGSVLHIYDALFQQDKVNHILVRHEQAATHAADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCC
YARATGKAGVVMVTSGPGATNAITGLATAFMDSIPMVVLSGQVRSDWIGDDAFQETDMIG
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCHHHHCCCCC
VSRPVVKHSFMVRDAKDIPEIMKKAFYIATTGRPGPVLVDIPKDQTAPNVKNPYEYPKKV
CCCHHHHHHHHHCCHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHH
KMRSYSPVSRGHSGQIRKALDELMKAKRPVIYAGGGVVQGEGSELLTQLAHRLNFPVTNT
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHHHCCCCHHHH
LMGLGGFPGTDRQNVGMLGMHGTYEANMTMHNSDVILAVGARLDDRVTNNVKKFCPGAKI
HHHCCCCCCCCCCCCCEEECCCEEECCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEE
IHIDIDPATISKNIMAHIPIVGPVQQVLEEMLAALDEHGGEPDRKALERWWEEIDGWRKV
EEEECCHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGMRYEINEDGPMKPQQVVQALYKATKGDAYVTSDVGQHQMFAAQYYQYDKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCC
LGTMGFGLPAAMGVQFAFPEATVACVTGEGSIQMNIQELSTCLQYGLPVKIININNQALG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHHH
MVRQWQDMQYGGRHSQSYMESLPDFVKLAESYGHVGMKITKIEDLDAAMDEAFALKDRLV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCEEE
FMDIYVDSTEHVYPMQIADGSMRDMWLSKTERT
EEEEEECCCCCEEEEEECCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRALQDEGVEYIFGYPGGSVLHIYDALFQQDKVNHILVRHEQAATHAADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCC
YARATGKAGVVMVTSGPGATNAITGLATAFMDSIPMVVLSGQVRSDWIGDDAFQETDMIG
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCHHHHCCCCC
VSRPVVKHSFMVRDAKDIPEIMKKAFYIATTGRPGPVLVDIPKDQTAPNVKNPYEYPKKV
CCCHHHHHHHHHCCHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHH
KMRSYSPVSRGHSGQIRKALDELMKAKRPVIYAGGGVVQGEGSELLTQLAHRLNFPVTNT
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHHHCCCCHHHH
LMGLGGFPGTDRQNVGMLGMHGTYEANMTMHNSDVILAVGARLDDRVTNNVKKFCPGAKI
HHHCCCCCCCCCCCCCEEECCCEEECCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEE
IHIDIDPATISKNIMAHIPIVGPVQQVLEEMLAALDEHGGEPDRKALERWWEEIDGWRKV
EEEECCHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGMRYEINEDGPMKPQQVVQALYKATKGDAYVTSDVGQHQMFAAQYYQYDKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCC
LGTMGFGLPAAMGVQFAFPEATVACVTGEGSIQMNIQELSTCLQYGLPVKIININNQALG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHHH
MVRQWQDMQYGGRHSQSYMESLPDFVKLAESYGHVGMKITKIEDLDAAMDEAFALKDRLV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCEEE
FMDIYVDSTEHVYPMQIADGSMRDMWLSKTERT
EEEEEECCCCCEEEEEECCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]