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The map label for this gene is gsiB [H]

Identifier: 110635961

GI number: 110635961

Start: 3926260

End: 3927807

Strand: Reverse

Name: gsiB [H]

Synonym: Meso_3635

Alternate gene names: 110635961

Gene position: 3927807-3926260 (Counterclockwise)

Preceding gene: 110635962

Following gene: 110635960

Centisome position: 89.02

GC content: 62.47

Gene sequence:

>1548_bases
ATGACAGAATTATATAAAATGAGAGGGCGCCGGGACATTTCACGTCGAGCCGTCCTGGCAGGCTCCGGCGGCCTCATTGG
AGCTCTACTCGCAACCCGGTTCGCAGCCGCGCAAAGCGAGCCGAAGCGCGGCGGAACGCTGCGCGTCGCCTTCTCCTCCA
CCCCCGACAGCCTCGACCCGCAGCGGACCCTCGCGGCGGCCGGGCAGCAAATCTCGAGCCTCGTCTTCGATAACCTGACC
CGCCTCGACGAAAACGGGATCGCCCAGCCCATGCTGGCCACGGCCTGGACCCCAGAGGCAGGAGGGGTTGAATGGGTGTT
CGAGCTGCGCCAGGGCGTCAAGTTCCATCACGGCACCGAGTTCACGTCGGCGGACGTAGTAGCGACCATCGAGCGCGCCT
ATGCGCCGGACTCCACCTTCCGCTCAAAGGGGGCCTTTGGGCCGATCAAGGAGGTAAAGGCTGAGGGCCCGTACAAGGTC
CGTTTCGTTCTCACGCAAGCCTTCGGCGAAATTCCGGTGGTGGTGGCCGGGCGCTGGTCGCGCATTATCGCCGCGGATGC
GATCGATACGCTGGAGACCGCGCCCTCGGGCACCGGGCCGTTCCTCTTCAAGTCGTTCGAAGCGGGCGCCAGTGTGAACG
TCGTGCGGAACCCCAGCTACTGGATCGAAGGCCAACCCTACCTCGACGGTGTGGAACTCGTCGGCATCAAGGAATCGATC
GCGCAGCAAGCGGCGATCCGCGGCGGCAACGTCGACGTTCTTACGCAGATCCCGGTCGAGACATTCCTGTCGCTGCGCAA
TGCCGATGGCATCAAGGTTTTCTCGAAGGTCACCGGCGCCTACCAAGTGATCATGATGCAGTCCAACATGGCGCCTTTCG
ATAACCCGAAGGTGCGCGAGGCGTTCCGCTACGTGCTGGACCGCGACCTGCTGGTGGCCTCGGCGCTGCTCGGTCAGGGT
CTCGTCGGCAACGACATGCCATTCCCGCCCGGCAGCGAATACCTGCCCGAAGTACCGCAATACAAGCAGGATCTCGATAA
GGCCAAAGCGCTTCTCAAGGAGGCCGGCCACGAGACAATGGACATCGAGCTCTTCACCAGCTCGGAACGTCCACCCACCC
CGAAGATCGCGCTCGCCTTCAAGGAGGCAGCGGCCAAGATCGGCGTCAACGTCACCATCACCGACGTTCCCTATGCCGAA
TACGCGGCCAACGTGGCGCGCAAGAAACCCTTCTACACGACGCAGTGGAGCGCCTATCCGACGATCTACGAGCGGCTGTA
CAACATGTACCGCAGCGGGGCGAACTTTAAGTACGGCGCCGTCGAGGACGCCGACGGTCTGGACGCGCTGCTCGACCAGA
TCATTTCCGAACTCGACGACGACAAGCGCAAGGAGCTTATCAAGGAAGCGCTGATCAAGATCCACAAGCATGGTGAGCGG
ATCATCCCCTACTTCACCATGTATGTCGGAGCCGCCAGCGAGCGGGTGCAGGCTTACACGCCGCCGACCTACGACATCAT
CGACTTCCGCGAAGTCTGGCTGTCGTAA

Upstream 100 bases:

>100_bases
GGTGGTCAAGGTCTGATTTGCACTGCCGGGAGTACGCTCCCCAATCACCTGAAGAAGCCGGTAAAAACCGGTCGCGGCAT
CCAAATCCAGAGGTGTGACG

Downstream 100 bases:

>100_bases
CTCGATGCCATCCTGTTCCGGGCCGATGTCATGACGGAGATGATCATCCGCAGGGTCGGGCTCCTCGCCCTCATCCTGTT
TGCCGTGTCGTTCATGACGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTELYKMRGRRDISRRAVLAGSGGLIGALLATRFAAAQSEPKRGGTLRVAFSSTPDSLDPQRTLAAAGQQISSLVFDNLT
RLDENGIAQPMLATAWTPEAGGVEWVFELRQGVKFHHGTEFTSADVVATIERAYAPDSTFRSKGAFGPIKEVKAEGPYKV
RFVLTQAFGEIPVVVAGRWSRIIAADAIDTLETAPSGTGPFLFKSFEAGASVNVVRNPSYWIEGQPYLDGVELVGIKESI
AQQAAIRGGNVDVLTQIPVETFLSLRNADGIKVFSKVTGAYQVIMMQSNMAPFDNPKVREAFRYVLDRDLLVASALLGQG
LVGNDMPFPPGSEYLPEVPQYKQDLDKAKALLKEAGHETMDIELFTSSERPPTPKIALAFKEAAAKIGVNVTITDVPYAE
YAANVARKKPFYTTQWSAYPTIYERLYNMYRSGANFKYGAVEDADGLDALLDQIISELDDDKRKELIKEALIKIHKHGER
IIPYFTMYVGAASERVQAYTPPTYDIIDFREVWLS

Sequences:

>Translated_515_residues
MTELYKMRGRRDISRRAVLAGSGGLIGALLATRFAAAQSEPKRGGTLRVAFSSTPDSLDPQRTLAAAGQQISSLVFDNLT
RLDENGIAQPMLATAWTPEAGGVEWVFELRQGVKFHHGTEFTSADVVATIERAYAPDSTFRSKGAFGPIKEVKAEGPYKV
RFVLTQAFGEIPVVVAGRWSRIIAADAIDTLETAPSGTGPFLFKSFEAGASVNVVRNPSYWIEGQPYLDGVELVGIKESI
AQQAAIRGGNVDVLTQIPVETFLSLRNADGIKVFSKVTGAYQVIMMQSNMAPFDNPKVREAFRYVLDRDLLVASALLGQG
LVGNDMPFPPGSEYLPEVPQYKQDLDKAKALLKEAGHETMDIELFTSSERPPTPKIALAFKEAAAKIGVNVTITDVPYAE
YAANVARKKPFYTTQWSAYPTIYERLYNMYRSGANFKYGAVEDADGLDALLDQIISELDDDKRKELIKEALIKIHKHGER
IIPYFTMYVGAASERVQAYTPPTYDIIDFREVWLS
>Mature_514_residues
TELYKMRGRRDISRRAVLAGSGGLIGALLATRFAAAQSEPKRGGTLRVAFSSTPDSLDPQRTLAAAGQQISSLVFDNLTR
LDENGIAQPMLATAWTPEAGGVEWVFELRQGVKFHHGTEFTSADVVATIERAYAPDSTFRSKGAFGPIKEVKAEGPYKVR
FVLTQAFGEIPVVVAGRWSRIIAADAIDTLETAPSGTGPFLFKSFEAGASVNVVRNPSYWIEGQPYLDGVELVGIKESIA
QQAAIRGGNVDVLTQIPVETFLSLRNADGIKVFSKVTGAYQVIMMQSNMAPFDNPKVREAFRYVLDRDLLVASALLGQGL
VGNDMPFPPGSEYLPEVPQYKQDLDKAKALLKEAGHETMDIELFTSSERPPTPKIALAFKEAAAKIGVNVTITDVPYAEY
AANVARKKPFYTTQWSAYPTIYERLYNMYRSGANFKYGAVEDADGLDALLDQIISELDDDKRKELIKEALIKIHKHGERI
IPYFTMYVGAASERVQAYTPPTYDIIDFREVWLS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=420, Percent_Identity=26.9047619047619, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1787762, Length=408, Percent_Identity=25.7352941176471, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789966, Length=531, Percent_Identity=24.6704331450094, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789887, Length=462, Percent_Identity=25.1082251082251, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786256, Length=306, Percent_Identity=22.8758169934641, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787551, Length=383, Percent_Identity=21.1488250652742, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56689; Mature: 56558

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELYKMRGRRDISRRAVLAGSGGLIGALLATRFAAAQSEPKRGGTLRVAFSSTPDSLDP
CCHHHHHHCCCCHHHCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCH
QRTLAAAGQQISSLVFDNLTRLDENGIAQPMLATAWTPEAGGVEWVFELRQGVKFHHGTE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEECCCCC
FTSADVVATIERAYAPDSTFRSKGAFGPIKEVKAEGPYKVRFVLTQAFGEIPVVVAGRWS
CCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCEEEECCCH
RIIAADAIDTLETAPSGTGPFLFKSFEAGASVNVVRNPSYWIEGQPYLDGVELVGIKESI
HHHHHHHHHHHHCCCCCCCCHHEEHHCCCCEEEEEECCCEEECCCCCCCCEEEECHHHHH
AQQAAIRGGNVDVLTQIPVETFLSLRNADGIKVFSKVTGAYQVIMMQSNMAPFDNPKVRE
HHHHHHCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
AFRYVLDRDLLVASALLGQGLVGNDMPFPPGSEYLPEVPQYKQDLDKAKALLKEAGHETM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
DIELFTSSERPPTPKIALAFKEAAAKIGVNVTITDVPYAEYAANVARKKPFYTTQWSAYP
EEEEEECCCCCCCCCEEHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCCH
TIYERLYNMYRSGANFKYGAVEDADGLDALLDQIISELDDDKRKELIKEALIKIHKHGER
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
IIPYFTMYVGAASERVQAYTPPTYDIIDFREVWLS
EHHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TELYKMRGRRDISRRAVLAGSGGLIGALLATRFAAAQSEPKRGGTLRVAFSSTPDSLDP
CHHHHHHCCCCHHHCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCH
QRTLAAAGQQISSLVFDNLTRLDENGIAQPMLATAWTPEAGGVEWVFELRQGVKFHHGTE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEECCCCC
FTSADVVATIERAYAPDSTFRSKGAFGPIKEVKAEGPYKVRFVLTQAFGEIPVVVAGRWS
CCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCEEEECCCH
RIIAADAIDTLETAPSGTGPFLFKSFEAGASVNVVRNPSYWIEGQPYLDGVELVGIKESI
HHHHHHHHHHHHCCCCCCCCHHEEHHCCCCEEEEEECCCEEECCCCCCCCEEEECHHHHH
AQQAAIRGGNVDVLTQIPVETFLSLRNADGIKVFSKVTGAYQVIMMQSNMAPFDNPKVRE
HHHHHHCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
AFRYVLDRDLLVASALLGQGLVGNDMPFPPGSEYLPEVPQYKQDLDKAKALLKEAGHETM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
DIELFTSSERPPTPKIALAFKEAAAKIGVNVTITDVPYAEYAANVARKKPFYTTQWSAYP
EEEEEECCCCCCCCCEEHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCCH
TIYERLYNMYRSGANFKYGAVEDADGLDALLDQIISELDDDKRKELIKEALIKIHKHGER
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
IIPYFTMYVGAASERVQAYTPPTYDIIDFREVWLS
EHHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA