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The map label for this gene is rhtB [C]

Identifier: 110635691

GI number: 110635691

Start: 3633151

End: 3633762

Strand: Reverse

Name: rhtB [C]

Synonym: Meso_3363

Alternate gene names: 110635691

Gene position: 3633762-3633151 (Counterclockwise)

Preceding gene: 110635692

Following gene: 161621420

Centisome position: 82.35

GC content: 62.42

Gene sequence:

>612_bases
ATGGATATTTCCTCGCTGATCATGTTCGCCGGCGTGCTCTTCGTAGCAGCCGGCTCTCCGGGCCCGAGCATAGCAGCGCT
TGTGGCGCGGGTCATCTCGCGCGGCTTCCGCGATGTGTTCCCGTTCCTGCTTGCCATGTGGATCGGCGAGGCGATCTGGC
TTTCCTGCGCGATTTTTGGCCTTGCCTTCATCGCCGAGACGTTCCACCTCGCGTTCCTCATCATCAAATATGTGGGCGTC
GCCTATCTTCTCTGGCTCGCCTGGAAGATGTGGACCGCGCCGGTCGCGGTGGAGCAAGGCGAGCTCCCGCGGGGGGATAA
CCGCTGGAAGCTGTTCCTCACCGGGATGGCTGTTACCCTCGGCAATCCGAAGATCATGATGTTTTACCTGGCGCTGCTGC
CCACGATCATCGATCTGTCGGCCGTGAGCCTGTTGGGCTGGGCCGAGCTGACGCTGACGATGCTTCTGGTGCTGGTTGCT
GTGGATTTCGCCTGGGCAATAGCAGCGGCTCAAGCCCGCCGCCTTTTGAGGAGCGCCCGCGCCATGCGCATCGCCAATCG
CACGAGTGCTTCATTGATGGCTGGGGCAGCGGCGGCGATCGCCAGCCGTTAG

Upstream 100 bases:

>100_bases
CTTCGAGAAGCAGAAGCTGGCAGTCGCCTAAGCGGCTGCGAAGGCTTTCCGTTCTGCTCGAAATTGATATGATGCCGCCG
CTTAACTGCCAGGCGGCATC

Downstream 100 bases:

>100_bases
GACGTTTCCGCCAGCGCCTTTTCGATCTCCGGCATCAGCTCGTTGCTTATGCTTTCGGGCGTGAAGGGGCCCACATGCTT
GTAGCGGATCTTGCCGTCGG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MDISSLIMFAGVLFVAAGSPGPSIAALVARVISRGFRDVFPFLLAMWIGEAIWLSCAIFGLAFIAETFHLAFLIIKYVGV
AYLLWLAWKMWTAPVAVEQGELPRGDNRWKLFLTGMAVTLGNPKIMMFYLALLPTIIDLSAVSLLGWAELTLTMLLVLVA
VDFAWAIAAAQARRLLRSARAMRIANRTSASLMAGAAAAIASR

Sequences:

>Translated_203_residues
MDISSLIMFAGVLFVAAGSPGPSIAALVARVISRGFRDVFPFLLAMWIGEAIWLSCAIFGLAFIAETFHLAFLIIKYVGV
AYLLWLAWKMWTAPVAVEQGELPRGDNRWKLFLTGMAVTLGNPKIMMFYLALLPTIIDLSAVSLLGWAELTLTMLLVLVA
VDFAWAIAAAQARRLLRSARAMRIANRTSASLMAGAAAAIASR
>Mature_203_residues
MDISSLIMFAGVLFVAAGSPGPSIAALVARVISRGFRDVFPFLLAMWIGEAIWLSCAIFGLAFIAETFHLAFLIIKYVGV
AYLLWLAWKMWTAPVAVEQGELPRGDNRWKLFLTGMAVTLGNPKIMMFYLALLPTIIDLSAVSLLGWAELTLTMLLVLVA
VDFAWAIAAAQARRLLRSARAMRIANRTSASLMAGAAAAIASR

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=184, Percent_Identity=27.1739130434783, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22021; Mature: 22021

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISSLIMFAGVLFVAAGSPGPSIAALVARVISRGFRDVFPFLLAMWIGEAIWLSCAIFG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFIAETFHLAFLIIKYVGVAYLLWLAWKMWTAPVAVEQGELPRGDNRWKLFLTGMAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEHHHCC
GNPKIMMFYLALLPTIIDLSAVSLLGWAELTLTMLLVLVAVDFAWAIAAAQARRLLRSAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMRIANRTSASLMAGAAAAIASR
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDISSLIMFAGVLFVAAGSPGPSIAALVARVISRGFRDVFPFLLAMWIGEAIWLSCAIFG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFIAETFHLAFLIIKYVGVAYLLWLAWKMWTAPVAVEQGELPRGDNRWKLFLTGMAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEHHHCC
GNPKIMMFYLALLPTIIDLSAVSLLGWAELTLTMLLVLVAVDFAWAIAAAQARRLLRSAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMRIANRTSASLMAGAAAAIASR
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]