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The map label for this gene is mcl2 [H]

Identifier: 110635597

GI number: 110635597

Start: 3534026

End: 3534919

Strand: Reverse

Name: mcl2 [H]

Synonym: Meso_3268

Alternate gene names: 110635597

Gene position: 3534919-3534026 (Counterclockwise)

Preceding gene: 110635599

Following gene: 110635596

Centisome position: 80.11

GC content: 63.65

Gene sequence:

>894_bases
ATGAAAGTGCGCATGCATGAGCCCCTGCCTCGCCTGCGCCGCTCCGTCCTTTATGTGCCCGCCAGCAACGAAAAGGCGCT
GCGCAAAAGCGCTGCGCTCCCCTGCGATGCCGTCATCTATGACCTGGAAGACGCGGTCGGGCCCGAGGAGAAAGAAGATG
CGCGCGAGCGCCTGCGCGGCTTTCTTGCCGGATCCGGGCGGCCGAAATGCGAGACCGTCATCCGCGTCAATGCACTGTCG
AGCGAATGGGGGACCGGGGACCTGCAGGTCGCAGGAGCCTGCCGGCCCGACGCAATCCTGCTGCCCAAAATAGATGGCCC
ACGCGATATTCTCGATGTCGATGCGGTGCTCGACGAGACGGATGCGCCGCGCTCGATCCGCCTTTGGGCCATGGTCGAAA
CCGCCCGCAGCGTGCTCAATATAGGCGCAATCGCGGAGTTGGGGCGCGATCCCGCCGCGCGGCTTTCCTGCCTCGTTGCC
GGTACGAACGACATTGCCAAGGAAACGCGCGTCACCAGCCGGATCAATATGGCGCCCTGGCTGATGCAGATCGTTCTTGC
CGCCCGTGCCGGAAACCTGACAGCGCTGGACGGGGTGTCCAACGATTTTCGCGATCTGGAAGCCTTCGCGCGGGAATGCG
CGGAAGCCCGCGCCATGGGATTCGACGGCAAGACGCTGATCCATCCCGCGCAGATCGATCCCGCCAACGCGGCCTTCTCG
CCCACAGCCGCGGAAATCGCGGAAGCGGAAGCTGTCGTCGAAGAGTTCTCGAAGCCGGAAAACCGCTTTCGGGGCGTCGT
CTCCATCCAGGGCCGCATGGTGGAGCGTCTCCATCTCGAACAATCGGAAGCGCTGCTCAAGAAGGCGCAGGAAATCAAGG
AGAGAGGTGAATGA

Upstream 100 bases:

>100_bases
TTTCCATGAACTTTTTGCCTAGCTGCAACACTTGGGTGACATCCCTTTACAACAACCATGCGGATACGCAAAGCCCTTGC
GCCCTTCTGCGCGCTGCGCC

Downstream 100 bases:

>100_bases
GGCTCTACCGCTTTCTGTCCGGTCCGGACGACTCAAGCTTCTGCCACAAGGTCACGGCCGCGCTGAACAAAGGCTGGCTG
CTCCACGGATCACCCACCTA

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKVRMHEPLPRLRRSVLYVPASNEKALRKSAALPCDAVIYDLEDAVGPEEKEDARERLRGFLAGSGRPKCETVIRVNALS
SEWGTGDLQVAGACRPDAILLPKIDGPRDILDVDAVLDETDAPRSIRLWAMVETARSVLNIGAIAELGRDPAARLSCLVA
GTNDIAKETRVTSRINMAPWLMQIVLAARAGNLTALDGVSNDFRDLEAFARECAEARAMGFDGKTLIHPAQIDPANAAFS
PTAAEIAEAEAVVEEFSKPENRFRGVVSIQGRMVERLHLEQSEALLKKAQEIKERGE

Sequences:

>Translated_297_residues
MKVRMHEPLPRLRRSVLYVPASNEKALRKSAALPCDAVIYDLEDAVGPEEKEDARERLRGFLAGSGRPKCETVIRVNALS
SEWGTGDLQVAGACRPDAILLPKIDGPRDILDVDAVLDETDAPRSIRLWAMVETARSVLNIGAIAELGRDPAARLSCLVA
GTNDIAKETRVTSRINMAPWLMQIVLAARAGNLTALDGVSNDFRDLEAFARECAEARAMGFDGKTLIHPAQIDPANAAFS
PTAAEIAEAEAVVEEFSKPENRFRGVVSIQGRMVERLHLEQSEALLKKAQEIKERGE
>Mature_297_residues
MKVRMHEPLPRLRRSVLYVPASNEKALRKSAALPCDAVIYDLEDAVGPEEKEDARERLRGFLAGSGRPKCETVIRVNALS
SEWGTGDLQVAGACRPDAILLPKIDGPRDILDVDAVLDETDAPRSIRLWAMVETARSVLNIGAIAELGRDPAARLSCLVA
GTNDIAKETRVTSRINMAPWLMQIVLAARAGNLTALDGVSNDFRDLEAFARECAEARAMGFDGKTLIHPAQIDPANAAFS
PTAAEIAEAEAVVEEFSKPENRFRGVVSIQGRMVERLHLEQSEALLKKAQEIKERGE

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=303, Percent_Identity=32.3432343234323, Blast_Score=137, Evalue=9e-33,
Organism=Escherichia coli, GI87081764, Length=289, Percent_Identity=33.2179930795848, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=29.4314381270903, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 32416; Mature: 32416

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRMHEPLPRLRRSVLYVPASNEKALRKSAALPCDAVIYDLEDAVGPEEKEDARERLRG
CCCCCCCCHHHHHHCEEEECCCCHHHHHHHHCCCHHHHEEEHHHHCCCCCHHHHHHHHHH
FLAGSGRPKCETVIRVNALSSEWGTGDLQVAGACRPDAILLPKIDGPRDILDVDAVLDET
HHCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCC
DAPRSIRLWAMVETARSVLNIGAIAELGRDPAARLSCLVAGTNDIAKETRVTSRINMAPW
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHHHCCHHHH
LMQIVLAARAGNLTALDGVSNDFRDLEAFARECAEARAMGFDGKTLIHPAQIDPANAAFS
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
PTAAEIAEAEAVVEEFSKPENRFRGVVSIQGRMVERLHLEQSEALLKKAQEIKERGE
CHHHHHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVRMHEPLPRLRRSVLYVPASNEKALRKSAALPCDAVIYDLEDAVGPEEKEDARERLRG
CCCCCCCCHHHHHHCEEEECCCCHHHHHHHHCCCHHHHEEEHHHHCCCCCHHHHHHHHHH
FLAGSGRPKCETVIRVNALSSEWGTGDLQVAGACRPDAILLPKIDGPRDILDVDAVLDET
HHCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCC
DAPRSIRLWAMVETARSVLNIGAIAELGRDPAARLSCLVAGTNDIAKETRVTSRINMAPW
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHHHCCHHHH
LMQIVLAARAGNLTALDGVSNDFRDLEAFARECAEARAMGFDGKTLIHPAQIDPANAAFS
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
PTAAEIAEAEAVVEEFSKPENRFRGVVSIQGRMVERLHLEQSEALLKKAQEIKERGE
CHHHHHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA