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The map label for this gene is amtB [H]

Identifier: 110635565

GI number: 110635565

Start: 3503603

End: 3504967

Strand: Reverse

Name: amtB [H]

Synonym: Meso_3236

Alternate gene names: 110635565

Gene position: 3504967-3503603 (Counterclockwise)

Preceding gene: 110635566

Following gene: 110635564

Centisome position: 79.43

GC content: 62.49

Gene sequence:

>1365_bases
ATGAGACTTTCCAGTATTGAAATTGCCAAGCGCATGGCCGGGGCGGGCGCGCTTGCGTTGGCGGCTTTCGCCGCTGTGCC
AGCTTTCGCGCAGGAAGCTGCGGAAACGGCTGCCGAGGCTGCCGCACCGGTGATGGACAAGGGCGATGTGGCCTGGATGA
TGACATCGACCCTTCTGGTGCTGTTCATGACGCTGCCGGGGCTGGCGCTCTTCTATGGCGGGCTGGTGCGCGCCAAAAAT
GCTCTTTCCGTGCTCATGCAGTGTACGATGATCGCCGCCGTGGTCATGGTGGTGTGGGTGCTCTGGGGCTATTCCTTTGC
CTTCGGTGGCGGCACCAGCCCCTTCTGGGGCGGCTTCGGCAAGCTGTTCCTATCGGGCGTGACGATGGATTCCACCGCCG
CAACCTTCACCGACGGCGTCGTCATCCCCGAATATGTCTTCATCTGCTTCCAGATGACATTCGCGGTGATCACGCCGGCG
CTGATCGTCGGCGCCTTTGCCGAACGCATCAAGTTTTCGGCGGTCGTGCTCTTCACGGTTCTCTGGGTCACCTTCGTCTA
TTTCCCGGTGGCCCATATGGTGTGGGACGGCAGCGGCTATCTGTTCAATCTCGGCGCTCTCGATTTCGCCGGCGGAACCG
TGGTCCACATCAATGCGGGCATCGCCGCTCTCGTAGGCTGCATGATCGTCGGCAAGCGCAGCGGCTACGGCAAGGATATT
CTCGCCCCGCATTCGATGACGCTTACCATGGTCGGCGGCTCCATCCTGTGGGTCGGCTGGTTCGGCTTCAATGCCGGGTC
GAACCTAGAAGCCACCGGCGGCGCTGCGCTTGCCATGATCAACACTTTCACCGCGACTGCCGGTGCGATTATCGGCTGGA
GCGTCATCGAGGCTCTCCATCGTGGCAAGGCCTCGATGCTGGGCGCCGTCTCCGGCCTGGTTGCCGGCCTCGTGGCCGTT
ACCCCCGCCTGCGGCTCGGTTGGTCCGGTGGGTGCCATTGTGCTCGGCGCCATAGCCAGCGCGGTCTGCTACTTCTTCGT
CGCAGTGGTGAAGAACAAGTTCGGCTATGACGACACGCTCGATGTTTTCGGGATCCACGGCGTGGGCGGTATCGTCGGCG
CGCTTGGCACGGGCATTTTCACCGCGCCCTGGCTTGGCGGCACGGGGGCCGATGATTTCTCCATTGCGTCGCAGCTTTGG
GTGCAGTTCGTGGCCGTGCTGGTCACCGTCGCATGGTGCGGGGTGGTTTCTGCGATCCTTTACAAGCTGGTTGATGCCAT
TGTCGGCCTGCGTCCGGAGAAGGAAGCCGAAACCCAGGGTCTGGACCTCACTTCGCACGGCGAGGCGGCCTATCATTACT
CCTGA

Upstream 100 bases:

>100_bases
ATCTTCGTCTACAGCATCGAAAAGGCAGTGCGTATCCGCACAGGCGAGACCGACGCCGATGCGCTGTGAGCGCCACGCGG
ATTTGTCCAGGGAGAATAGA

Downstream 100 bases:

>100_bases
CCGGCGGCATCTCTCTTCCTTAGGCGCGTAAACTGGGCCCGGCGAAAGCTGGGCCCCTTTTTTGGGGGGAAGCGAATAGG
GAATGGCGAGCAGGGAATCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRLSSIEIAKRMAGAGALALAAFAAVPAFAQEAAETAAEAAAPVMDKGDVAWMMTSTLLVLFMTLPGLALFYGGLVRAKN
ALSVLMQCTMIAAVVMVVWVLWGYSFAFGGGTSPFWGGFGKLFLSGVTMDSTAATFTDGVVIPEYVFICFQMTFAVITPA
LIVGAFAERIKFSAVVLFTVLWVTFVYFPVAHMVWDGSGYLFNLGALDFAGGTVVHINAGIAALVGCMIVGKRSGYGKDI
LAPHSMTLTMVGGSILWVGWFGFNAGSNLEATGGAALAMINTFTATAGAIIGWSVIEALHRGKASMLGAVSGLVAGLVAV
TPACGSVGPVGAIVLGAIASAVCYFFVAVVKNKFGYDDTLDVFGIHGVGGIVGALGTGIFTAPWLGGTGADDFSIASQLW
VQFVAVLVTVAWCGVVSAILYKLVDAIVGLRPEKEAETQGLDLTSHGEAAYHYS

Sequences:

>Translated_454_residues
MRLSSIEIAKRMAGAGALALAAFAAVPAFAQEAAETAAEAAAPVMDKGDVAWMMTSTLLVLFMTLPGLALFYGGLVRAKN
ALSVLMQCTMIAAVVMVVWVLWGYSFAFGGGTSPFWGGFGKLFLSGVTMDSTAATFTDGVVIPEYVFICFQMTFAVITPA
LIVGAFAERIKFSAVVLFTVLWVTFVYFPVAHMVWDGSGYLFNLGALDFAGGTVVHINAGIAALVGCMIVGKRSGYGKDI
LAPHSMTLTMVGGSILWVGWFGFNAGSNLEATGGAALAMINTFTATAGAIIGWSVIEALHRGKASMLGAVSGLVAGLVAV
TPACGSVGPVGAIVLGAIASAVCYFFVAVVKNKFGYDDTLDVFGIHGVGGIVGALGTGIFTAPWLGGTGADDFSIASQLW
VQFVAVLVTVAWCGVVSAILYKLVDAIVGLRPEKEAETQGLDLTSHGEAAYHYS
>Mature_454_residues
MRLSSIEIAKRMAGAGALALAAFAAVPAFAQEAAETAAEAAAPVMDKGDVAWMMTSTLLVLFMTLPGLALFYGGLVRAKN
ALSVLMQCTMIAAVVMVVWVLWGYSFAFGGGTSPFWGGFGKLFLSGVTMDSTAATFTDGVVIPEYVFICFQMTFAVITPA
LIVGAFAERIKFSAVVLFTVLWVTFVYFPVAHMVWDGSGYLFNLGALDFAGGTVVHINAGIAALVGCMIVGKRSGYGKDI
LAPHSMTLTMVGGSILWVGWFGFNAGSNLEATGGAALAMINTFTATAGAIIGWSVIEALHRGKASMLGAVSGLVAGLVAV
TPACGSVGPVGAIVLGAIASAVCYFFVAVVKNKFGYDDTLDVFGIHGVGGIVGALGTGIFTAPWLGGTGADDFSIASQLW
VQFVAVLVTVAWCGVVSAILYKLVDAIVGLRPEKEAETQGLDLTSHGEAAYHYS

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=434, Percent_Identity=50.6912442396313, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI133931100, Length=431, Percent_Identity=27.3781902552204, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=439, Percent_Identity=27.7904328018223, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=438, Percent_Identity=29.2237442922374, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=26.9585253456221, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=425, Percent_Identity=35.2941176470588, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=32.8571428571429, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=434, Percent_Identity=30.8755760368664, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=27.9518072289157, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46967; Mature: 46967

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]