Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 110635562

GI number: 110635562

Start: 3499198

End: 3500052

Strand: Reverse

Name: xthA [H]

Synonym: Meso_3233

Alternate gene names: 110635562

Gene position: 3500052-3499198 (Counterclockwise)

Preceding gene: 110635563

Following gene: 110635561

Centisome position: 79.32

GC content: 61.52

Gene sequence:

>855_bases
TTGTCATCAGCGCTCGCAAAGGTTTATTCCGGTCCTGAAAAACCGGAACTTTCGATGCCCTTCAAAGTCGCCACCTGGAA
CATAAACTCCGTCCGCCTCCGCCTTCCGCTCGTCCTGCGCTTTCTTGAGCAATACCAGCCCGACGTGCTGTGCCTGCAGG
AGACGAAGTGCCCTGACGACCAGTTCCCCACGGCCGCCTTCCGCAAGGTCGGCTATGGCCACGTCGAAATCCATGGCCAA
AAGGGATATCACGGCGTGGCGACGATCTCGCGCCTGCCGCTGGAGGTGGTGGAGCGCCGCAGCTTTTGCGGCCTTACCGA
TTGCCGCCACCTCTCGACGCTGACGAATGTGGACGGACGGCGGGTCCTGCTGCACAATTTCTATGTGCCGGCAGGCGGGG
ACGAGCCCGACCCCGAAATCAATCCGAAATTTCGCCACAAGCTGGACTTCCTGCAGGAAATGCGGGACATCATACCCACC
CTGGATGCGAATACGGGCTCGATCCTCGTCGGCGATCTTAATGTGGCTCCGCTTGAGACCGATGTCTGGTCGCATAAGCA
GATGCTGAATGTGGTGAGCCACACGCCCGTCGAGACCGAAGGCCTGGAGTCCGTGCGCAAGGGAGGCGGATGGGTGGATC
TGATGCGCCGGCACATCCCGCCCGAGGAAAAGCTCTTCACCTGGTGGAGCTACCGAGCGAAGGATTGGAGCCTTTCCAAT
CGCGGCCGTCGGCTCGACCATGTCTGGTCGTCCGATGACCTTGCGCCGGCCCTCAGCCGGGTAGACGTGCTGCGGGATGC
GCGCGGTTGGGACCGTCCTTCCGATCATGTGCCCGTGATCGCCGCATTCGGCTGA

Upstream 100 bases:

>100_bases
TTTACATCGATCATCGGCGCGTGAACGCGATGGCGCGCGGCGAAAGATAAGGCCGAAATTTCAGCTTCGCCCTTAACGGC
AAATCTTTCCTTGCGGGCGC

Downstream 100 bases:

>100_bases
TCCCGCCCATGCCGCCGTCTTCCTGGTCTCGCCGGAGCAAGAACGGTATCGAAATCTTCGTGCGGCTCACGCCGAAATCC
TCCTCGGACGCCATTGAAGG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSSALAKVYSGPEKPELSMPFKVATWNINSVRLRLPLVLRFLEQYQPDVLCLQETKCPDDQFPTAAFRKVGYGHVEIHGQ
KGYHGVATISRLPLEVVERRSFCGLTDCRHLSTLTNVDGRRVLLHNFYVPAGGDEPDPEINPKFRHKLDFLQEMRDIIPT
LDANTGSILVGDLNVAPLETDVWSHKQMLNVVSHTPVETEGLESVRKGGGWVDLMRRHIPPEEKLFTWWSYRAKDWSLSN
RGRRLDHVWSSDDLAPALSRVDVLRDARGWDRPSDHVPVIAAFG

Sequences:

>Translated_284_residues
MSSALAKVYSGPEKPELSMPFKVATWNINSVRLRLPLVLRFLEQYQPDVLCLQETKCPDDQFPTAAFRKVGYGHVEIHGQ
KGYHGVATISRLPLEVVERRSFCGLTDCRHLSTLTNVDGRRVLLHNFYVPAGGDEPDPEINPKFRHKLDFLQEMRDIIPT
LDANTGSILVGDLNVAPLETDVWSHKQMLNVVSHTPVETEGLESVRKGGGWVDLMRRHIPPEEKLFTWWSYRAKDWSLSN
RGRRLDHVWSSDDLAPALSRVDVLRDARGWDRPSDHVPVIAAFG
>Mature_283_residues
SSALAKVYSGPEKPELSMPFKVATWNINSVRLRLPLVLRFLEQYQPDVLCLQETKCPDDQFPTAAFRKVGYGHVEIHGQK
GYHGVATISRLPLEVVERRSFCGLTDCRHLSTLTNVDGRRVLLHNFYVPAGGDEPDPEINPKFRHKLDFLQEMRDIIPTL
DANTGSILVGDLNVAPLETDVWSHKQMLNVVSHTPVETEGLESVRKGGGWVDLMRRHIPPEEKLFTWWSYRAKDWSLSNR
GRRLDHVWSSDDLAPALSRVDVLRDARGWDRPSDHVPVIAAFG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=271, Percent_Identity=28.7822878228782, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375503, Length=271, Percent_Identity=28.7822878228782, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375501, Length=271, Percent_Identity=28.7822878228782, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788046, Length=275, Percent_Identity=32.7272727272727, Blast_Score=118, Evalue=4e-28,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=27.5471698113208, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=27.5471698113208, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32238; Mature: 32107

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSALAKVYSGPEKPELSMPFKVATWNINSVRLRLPLVLRFLEQYQPDVLCLQETKCPDD
CCHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCC
QFPTAAFRKVGYGHVEIHGQKGYHGVATISRLPLEVVERRSFCGLTDCRHLSTLTNVDGR
CCCHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCC
RVLLHNFYVPAGGDEPDPEINPKFRHKLDFLQEMRDIIPTLDANTGSILVGDLNVAPLET
EEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
DVWSHKQMLNVVSHTPVETEGLESVRKGGGWVDLMRRHIPPEEKLFTWWSYRAKDWSLSN
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHEEEEEECCCCCCCCCC
RGRRLDHVWSSDDLAPALSRVDVLRDARGWDRPSDHVPVIAAFG
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SSALAKVYSGPEKPELSMPFKVATWNINSVRLRLPLVLRFLEQYQPDVLCLQETKCPDD
CHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCC
QFPTAAFRKVGYGHVEIHGQKGYHGVATISRLPLEVVERRSFCGLTDCRHLSTLTNVDGR
CCCHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCC
RVLLHNFYVPAGGDEPDPEINPKFRHKLDFLQEMRDIIPTLDANTGSILVGDLNVAPLET
EEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
DVWSHKQMLNVVSHTPVETEGLESVRKGGGWVDLMRRHIPPEEKLFTWWSYRAKDWSLSN
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHEEEEEECCCCCCCCCC
RGRRLDHVWSSDDLAPALSRVDVLRDARGWDRPSDHVPVIAAFG
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]