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The map label for this gene is tolB

Identifier: 110635500

GI number: 110635500

Start: 3444168

End: 3445487

Strand: Reverse

Name: tolB

Synonym: Meso_3171

Alternate gene names: 110635500

Gene position: 3445487-3444168 (Counterclockwise)

Preceding gene: 110635501

Following gene: 110635499

Centisome position: 78.09

GC content: 60.61

Gene sequence:

>1320_bases
ATGCGGAACTTTTTCAAAACGGCCGTCGCGATTACAAGCCTTGTCATGGGGCTCGTAGCAATGGCTGTCGTGCCGGCGCG
CGCGCTTGTGGAAATCGATGTCAATCGCGGCGTCGTCGAGCCGGTGCCGATCGCCATCACGGACTTCCTGTCCGCTGGAG
GGCGGGGCGCCGAGATATCCGCCGTGATCGCCGCCGATCTTAAGCGTTCCGGCCTTTTCGCGCCCATAGACCGCGGCGCC
TTCATCGAGAAGATTTCCAATCCGGACGTGGCTCCGCGTTTCGAGGATTGGAAGGTCATCAATGCGCAGGCGATCGTCAC
GGGCCGCGTGACCGAGGAGCCAGGCGGCAGGCTCAAGGCCGAGTTCCGCCTTTGGGACACCTTCGCCGGACAGCAGCTCG
TGGGCGAGCAGTTCTTTGCCAAGCCGGCGGATTGGCGCCGCGTCGCGCATATCATTGCCGATACGATCTATGAGCAGTTC
ACCGGCGAGAAGGGCTATTTCGACAGCCGCATCGTTTTCGTGGACGAGTCCGGGTCGAAGGAAAACCGCGTGAAACGGTT
GGCCATTATGGATCAGGACGGGGCCAATTACAGGGCGCTGACCGATGGCCGCTCCATCGTGCTGAAGCCGAGCTTCTCGC
CTCAGCGGCAGGAAATCACCTATGTGTCCTTCGAAGGGGGGCAGCCTCAGGTCTATCTGCTGCAAATCGAAACCGGCCAG
CGCGAGCTTGTGGGAAACAATTTCCCGACAATGAGCTTCGCGTCGCGCTTCAATCCGGTCGGCGACAAGATCATCATGAG
CCTGCTTCGTACGGACGGCAATTCAAACATCTATACCATGGATCTGAAGACGCGGACCACCGCGCGGCTCACGGATACCA
ATGCCATCGACACCTCCCCATCCTATTCGCCCGATGGCAACAAGGTGGTCTTCACCTCCGATCGTGGCGGGCGGGCGCAA
ATCTATGTGATGAATGCTGATGGTTCCAACCCGCAGCGCATCTCCTTCGGCGACGGCACCTATTCAGAACCCGTGTGGTC
TCCGCGAGGCGATCTGATCGCGTTCACCAAACAGTCCGGCGGCCAGTTCCAGATCGGCATCATGCGCACGGATGGATCCG
GGGAACGCATTCTGTCCACCGGTTTCCAGATCGAAGGGCCGACCTGGTCCCCGAACGGTCGGGTCATCATGTTCTTCCGG
CAGGATGCCGGGGCGGCGGGGCCGAAGCTCTATTCGATCGACCTTACGGGCCGTAACGAGCAACTCATCCCGACGCCGAA
TTTCGCCTCCGATCCCGCGTGGTCTCCGCTACTGAACTAG

Upstream 100 bases:

>100_bases
CCATCCGAAAAATATAGCGCCTGGGCCGATGTCATCGTCCATTTCGACCCAACCGATATGTTCTGATCCACAGGATCTCT
CCATCAAGAGGCATGAGCTG

Downstream 100 bases:

>100_bases
AGCGCTGCCGCCTTTCAGCCGTATTTGCCGGTACGGTCAGGTCACATGCTTGGTGAGTCTGCCGAGCGGCAAGGAAAAAT
TAACCCTGTTTCTTGAGCGC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRNFFKTAVAITSLVMGLVAMAVVPARALVEIDVNRGVVEPVPIAITDFLSAGGRGAEISAVIAADLKRSGLFAPIDRGA
FIEKISNPDVAPRFEDWKVINAQAIVTGRVTEEPGGRLKAEFRLWDTFAGQQLVGEQFFAKPADWRRVAHIIADTIYEQF
TGEKGYFDSRIVFVDESGSKENRVKRLAIMDQDGANYRALTDGRSIVLKPSFSPQRQEITYVSFEGGQPQVYLLQIETGQ
RELVGNNFPTMSFASRFNPVGDKIIMSLLRTDGNSNIYTMDLKTRTTARLTDTNAIDTSPSYSPDGNKVVFTSDRGGRAQ
IYVMNADGSNPQRISFGDGTYSEPVWSPRGDLIAFTKQSGGQFQIGIMRTDGSGERILSTGFQIEGPTWSPNGRVIMFFR
QDAGAAGPKLYSIDLTGRNEQLIPTPNFASDPAWSPLLN

Sequences:

>Translated_439_residues
MRNFFKTAVAITSLVMGLVAMAVVPARALVEIDVNRGVVEPVPIAITDFLSAGGRGAEISAVIAADLKRSGLFAPIDRGA
FIEKISNPDVAPRFEDWKVINAQAIVTGRVTEEPGGRLKAEFRLWDTFAGQQLVGEQFFAKPADWRRVAHIIADTIYEQF
TGEKGYFDSRIVFVDESGSKENRVKRLAIMDQDGANYRALTDGRSIVLKPSFSPQRQEITYVSFEGGQPQVYLLQIETGQ
RELVGNNFPTMSFASRFNPVGDKIIMSLLRTDGNSNIYTMDLKTRTTARLTDTNAIDTSPSYSPDGNKVVFTSDRGGRAQ
IYVMNADGSNPQRISFGDGTYSEPVWSPRGDLIAFTKQSGGQFQIGIMRTDGSGERILSTGFQIEGPTWSPNGRVIMFFR
QDAGAAGPKLYSIDLTGRNEQLIPTPNFASDPAWSPLLN
>Mature_439_residues
MRNFFKTAVAITSLVMGLVAMAVVPARALVEIDVNRGVVEPVPIAITDFLSAGGRGAEISAVIAADLKRSGLFAPIDRGA
FIEKISNPDVAPRFEDWKVINAQAIVTGRVTEEPGGRLKAEFRLWDTFAGQQLVGEQFFAKPADWRRVAHIIADTIYEQF
TGEKGYFDSRIVFVDESGSKENRVKRLAIMDQDGANYRALTDGRSIVLKPSFSPQRQEITYVSFEGGQPQVYLLQIETGQ
RELVGNNFPTMSFASRFNPVGDKIIMSLLRTDGNSNIYTMDLKTRTTARLTDTNAIDTSPSYSPDGNKVVFTSDRGGRAQ
IYVMNADGSNPQRISFGDGTYSEPVWSPRGDLIAFTKQSGGQFQIGIMRTDGSGERILSTGFQIEGPTWSPNGRVIMFFR
QDAGAAGPKLYSIDLTGRNEQLIPTPNFASDPAWSPLLN

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=431, Percent_Identity=30.1624129930394, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_MESSB (Q11DI2)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_675708.1
- ProteinModelPortal:   Q11DI2
- SMR:   Q11DI2
- STRING:   Q11DI2
- GeneID:   4181831
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_3171
- NMPDR:   fig|266779.1.peg.114
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q11DI2
- ProtClustDB:   PRK05137
- BioCyc:   MSP266779:MESO_3171-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48206; Mature: 48206

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFFKTAVAITSLVMGLVAMAVVPARALVEIDVNRGVVEPVPIAITDFLSAGGRGAEIS
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEE
AVIAADLKRSGLFAPIDRGAFIEKISNPDVAPRFEDWKVINAQAIVTGRVTEEPGGRLKA
EEEEEHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCEEE
EFRLWDTFAGQQLVGEQFFAKPADWRRVAHIIADTIYEQFTGEKGYFDSRIVFVDESGSK
EEEEEEHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
ENRVKRLAIMDQDGANYRALTDGRSIVLKPSFSPQRQEITYVSFEGGQPQVYLLQIETGQ
HHHEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCC
RELVGNNFPTMSFASRFNPVGDKIIMSLLRTDGNSNIYTMDLKTRTTARLTDTNAIDTSP
HHHCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCC
SYSPDGNKVVFTSDRGGRAQIYVMNADGSNPQRISFGDGTYSEPVWSPRGDLIAFTKQSG
CCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCC
GQFQIGIMRTDGSGERILSTGFQIEGPTWSPNGRVIMFFRQDAGAAGPKLYSIDLTGRNE
CEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCC
QLIPTPNFASDPAWSPLLN
EEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNFFKTAVAITSLVMGLVAMAVVPARALVEIDVNRGVVEPVPIAITDFLSAGGRGAEIS
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEE
AVIAADLKRSGLFAPIDRGAFIEKISNPDVAPRFEDWKVINAQAIVTGRVTEEPGGRLKA
EEEEEHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCEEE
EFRLWDTFAGQQLVGEQFFAKPADWRRVAHIIADTIYEQFTGEKGYFDSRIVFVDESGSK
EEEEEEHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
ENRVKRLAIMDQDGANYRALTDGRSIVLKPSFSPQRQEITYVSFEGGQPQVYLLQIETGQ
HHHEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCC
RELVGNNFPTMSFASRFNPVGDKIIMSLLRTDGNSNIYTMDLKTRTTARLTDTNAIDTSP
HHHCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCC
SYSPDGNKVVFTSDRGGRAQIYVMNADGSNPQRISFGDGTYSEPVWSPRGDLIAFTKQSG
CCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCC
GQFQIGIMRTDGSGERILSTGFQIEGPTWSPNGRVIMFFRQDAGAAGPKLYSIDLTGRNE
CEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCC
QLIPTPNFASDPAWSPLLN
EEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA