| Definition | Mesorhizobium sp. BNC1, complete genome. |
|---|---|
| Accession | NC_008254 |
| Length | 4,412,446 |
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The map label for this gene is dkgB [H]
Identifier: 110635381
GI number: 110635381
Start: 3326959
End: 3327783
Strand: Reverse
Name: dkgB [H]
Synonym: Meso_3052
Alternate gene names: 110635381
Gene position: 3327783-3326959 (Counterclockwise)
Preceding gene: 110635383
Following gene: 110635380
Centisome position: 75.42
GC content: 62.91
Gene sequence:
>825_bases ATGCAAACCGTCACCGCTCACGGCGCGACCATCCCTGCTCTCGGCTTCGGCGTCTTCCGAATGTCCGACGCAGAGGTGGA GCGCATTATCCCGGCCGCGCTCGAGGCCGGCTTCCGCCACTTCGACACTGCTCAAATCTACCAGAACGAAGCCGCGCTCG GCCGGGCGCTGGAGCGTGCCGGTGCCCGCCGCGAGGATTTGTTTCTGACCACCAAGGTATGGGTCGATAACTATAGCGCC GACAAGTTCGCCGCTTCTGTCGACGAAAGCCTCGAAAAGCTGAAGGTCGATCAGGTTGATCTCCTTCTCCTGCATTGGCC GGCCCATGAGGTTCCGGTGGCGGCGCAGATCGAGAGGTTGAATGCCGTGAAGGACGCCGGAAAGACCCGGTTTATCGGCG TATCCAACCAGAACATCGCGCAGATGCGGGAGTCGATCGCCCGCTCGGCCGCGCCGATCGTCACCAACCAGGTGGAATTG CATCCCTATCTGCCCCAGCACTCGCTTGTCGCGGCCGCAAGGGACAGCGCTGTCGCAGTCACCGCCTATTACGGCATGGC CGACGGCGCTGTGCCGAAGGACCCGGTACTGCAGCAGATCGGCGCCAGATACGGCAAGAGCGCCGCACAGGTTGGGCTAC GCTGGCTTGTCCAGCAGGGCTATGTCGCCCTTTCCAAAACGGCTAACCCTGCACGCGTCGCGGAAAACATCGCGATCTTC GATTTCGCCCTTGATGAAACCGATATGGCCGCCATCGCCAAGCTTGCCCGCCCCGACGGGCGGCTCGTCAGCCCTCCCGG CCTTGCTCCTGATTGGGACGCCTAA
Upstream 100 bases:
>100_bases ACAAACGCGTCCGCGCTCGATTCCACCCCGCATTCCCGGCGAATGGCGCTCGGGATCCGACACGGCAAGGCCGTTTGACC AATCCAGAAGGACTTTCGCA
Downstream 100 bases:
>100_bases GGAGCTTCAATCATGAACGCCAATGTCTCGACGGCCGGAACGACGGCCGAAGCATCCTCGTCCCCCCGCTGGGCGCTTCT CGCTCTTGCGATCGGCGCTT
Product: aldo/keto reductase
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 274; Mature: 274
Protein sequence:
>274_residues MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA
Sequences:
>Translated_274_residues MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA >Mature_274_residues MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=284, Percent_Identity=29.5774647887324, Blast_Score=153, Evalue=2e-37, Organism=Homo sapiens, GI24497585, Length=289, Percent_Identity=31.8339100346021, Blast_Score=151, Evalue=6e-37, Organism=Homo sapiens, GI5174695, Length=290, Percent_Identity=31.3793103448276, Blast_Score=150, Evalue=9e-37, Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=30.8474576271186, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=30.7958477508651, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI310109922, Length=270, Percent_Identity=31.4814814814815, Blast_Score=149, Evalue=3e-36, Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=30.4498269896194, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=30.1038062283737, Blast_Score=144, Evalue=7e-35, Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=30.1038062283737, Blast_Score=144, Evalue=7e-35, Organism=Homo sapiens, GI24497583, Length=292, Percent_Identity=30.8219178082192, Blast_Score=141, Evalue=6e-34, Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=29.7945205479452, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=29.7945205479452, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI291291012, Length=269, Percent_Identity=28.6245353159851, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=29.9651567944251, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI300116273, Length=272, Percent_Identity=30.1470588235294, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI300116271, Length=269, Percent_Identity=30.1115241635688, Blast_Score=125, Evalue=3e-29, Organism=Homo sapiens, GI310109924, Length=151, Percent_Identity=31.1258278145695, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI310109926, Length=107, Percent_Identity=35.5140186915888, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI207028673, Length=107, Percent_Identity=34.5794392523364, Blast_Score=78, Evalue=9e-15, Organism=Homo sapiens, GI27436969, Length=290, Percent_Identity=23.7931034482759, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI4504825, Length=290, Percent_Identity=23.7931034482759, Blast_Score=67, Evalue=1e-11, Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=43.609022556391, Blast_Score=211, Evalue=3e-56, Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=35.2490421455939, Blast_Score=169, Evalue=1e-43, Organism=Escherichia coli, GI1788081, Length=265, Percent_Identity=32.0754716981132, Blast_Score=94, Evalue=8e-21, Organism=Escherichia coli, GI87081735, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=2e-15, Organism=Escherichia coli, GI1788070, Length=312, Percent_Identity=24.3589743589744, Blast_Score=75, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17550248, Length=279, Percent_Identity=34.0501792114695, Blast_Score=153, Evalue=7e-38, Organism=Caenorhabditis elegans, GI17537075, Length=292, Percent_Identity=32.1917808219178, Blast_Score=145, Evalue=3e-35, Organism=Caenorhabditis elegans, GI17537077, Length=279, Percent_Identity=31.8996415770609, Blast_Score=144, Evalue=4e-35, Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=31.5985130111524, Blast_Score=142, Evalue=2e-34, Organism=Caenorhabditis elegans, GI17564128, Length=284, Percent_Identity=31.6901408450704, Blast_Score=142, Evalue=2e-34, Organism=Caenorhabditis elegans, GI17566692, Length=278, Percent_Identity=32.0143884892086, Blast_Score=141, Evalue=3e-34, Organism=Caenorhabditis elegans, GI17552492, Length=258, Percent_Identity=32.1705426356589, Blast_Score=139, Evalue=2e-33, Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=29.368029739777, Blast_Score=135, Evalue=2e-32, Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=29.7029702970297, Blast_Score=130, Evalue=7e-31, Organism=Caenorhabditis elegans, GI17561300, Length=266, Percent_Identity=28.9473684210526, Blast_Score=121, Evalue=4e-28, Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=30.03663003663, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17562292, Length=288, Percent_Identity=26.7361111111111, Blast_Score=110, Evalue=5e-25, Organism=Saccharomyces cerevisiae, GI6320576, Length=285, Percent_Identity=34.7368421052632, Blast_Score=172, Evalue=5e-44, Organism=Saccharomyces cerevisiae, GI6324694, Length=291, Percent_Identity=34.020618556701, Blast_Score=167, Evalue=2e-42, Organism=Saccharomyces cerevisiae, GI6319625, Length=279, Percent_Identity=30.4659498207885, Blast_Score=128, Evalue=9e-31, Organism=Saccharomyces cerevisiae, GI6322556, Length=265, Percent_Identity=26.7924528301887, Blast_Score=119, Evalue=5e-28, Organism=Saccharomyces cerevisiae, GI6320079, Length=249, Percent_Identity=28.5140562248996, Blast_Score=96, Evalue=7e-21, Organism=Drosophila melanogaster, GI24657054, Length=277, Percent_Identity=32.8519855595668, Blast_Score=159, Evalue=2e-39, Organism=Drosophila melanogaster, GI21356425, Length=278, Percent_Identity=33.8129496402878, Blast_Score=157, Evalue=6e-39, Organism=Drosophila melanogaster, GI24644950, Length=290, Percent_Identity=34.1379310344828, Blast_Score=155, Evalue=2e-38, Organism=Drosophila melanogaster, GI221378297, Length=310, Percent_Identity=31.2903225806452, Blast_Score=152, Evalue=2e-37, Organism=Drosophila melanogaster, GI24663317, Length=281, Percent_Identity=32.0284697508897, Blast_Score=144, Evalue=5e-35, Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=30.5084745762712, Blast_Score=143, Evalue=2e-34, Organism=Drosophila melanogaster, GI20129731, Length=274, Percent_Identity=32.4817518248175, Blast_Score=138, Evalue=4e-33, Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=30.9090909090909, Blast_Score=128, Evalue=5e-30, Organism=Drosophila melanogaster, GI24662785, Length=295, Percent_Identity=29.4915254237288, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=28.6206896551724, Blast_Score=117, Evalue=6e-27, Organism=Drosophila melanogaster, GI281366140, Length=240, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=4e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29639; Mature: 29639
Theoretical pI: Translated: 5.96; Mature: 5.96
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERA CCEEECCCCCCHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH GARREDLFLTTKVWVDNYSADKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERL CCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH NAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVELHPYLPQHSLVAAARDSAVAV HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEECEEEECCCCCCHHHHHHHCCCEEEE TAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF EEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCEEEE DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA EEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCC >Mature Secondary Structure MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERA CCEEECCCCCCHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH GARREDLFLTTKVWVDNYSADKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERL CCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH NAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVELHPYLPQHSLVAAARDSAVAV HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEECEEEECCCCCCHHHHHHHCCCEEEE TAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF EEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCEEEE DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA EEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]