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The map label for this gene is glgX [H]

Identifier: 110635010

GI number: 110635010

Start: 2905465

End: 2907540

Strand: Reverse

Name: glgX [H]

Synonym: Meso_2676

Alternate gene names: 110635010

Gene position: 2907540-2905465 (Counterclockwise)

Preceding gene: 110635011

Following gene: 110635009

Centisome position: 65.89

GC content: 61.37

Gene sequence:

>2076_bases
GTGCGTGTAGAAATTGGCCTTCCATACCCGCTCGGCGCGACATGGGACGGGTCGGGCGTCAATGTCGCCGTCTTCTCGGC
CCATGCCGAAAAGATCGAGCTTTGCCTTTTCGACGCCCAGGGGCGGCGGGAAATACGCCGCATTCCCTTACCCGAGTACA
CGCATGAGGTTTGGCACGGATATTTTCCGGACATGCGGCCGGGACAGGTCTACGGACTGCGAGCCTACGGCCCATACGAT
CCTGCAAACGGCCACCGCTTCAATCCCAACAAGCTTCTGATCGATCCTTACGCGAAACAGCTCTTGGGCGATATCCGCTG
GCACGACGCCTGCTTCGGCTACCGCGTGGGAGGCGCGCGGCAGGACCTCGCGATAGACCGTCGCGATTCGGCTTTTGTCA
TGCCGAAATGCGTGGTGACCGATACGGCGGCCACCTGGGGCAATGATGTGCGCCCGCACCGGCCCTGGGAAGAGACGATC
ATCTATGAGGCCCATGTGAAGGGCATGACCGCGCTGCATCCGGACGTGCCGGAGCAATTCCGCGGCACGTTCGGCGGCCT
GGCCGACTCCCGTGTCATCGATTATCTCGTCAAACTCGGCATAACGGCGATTGAGCTCCTGCCCGTGCAGGCCTTCTTCG
ACGACCGGTATCTTGTGGAAAAGCGCCTCACGAATTACTGGGGCTACAATACGATCGGCTTTTTCGCGCCGACACCGCGC
TACATCTCACCGAGAGGCGGACTGCACGAGTTCAAGCGCATGGTGCGCAAGCTCCACGAAGCGGGCATCGAGGTGATCCT
CGACGTGGTTTATAACCATACGGCGGAAGGCAATGAGCTCGGCCCCACGCTTTCCTTCCGCGGGCTCGACAATGCCAGCT
ACTACATCCTGGGTGACGACCCCCGCTACTACTACGACACCACGGGCTGCGGAAATACGCTCAATCTCAAGCATCCGCGC
GTGCTGCAGATGGTGATGGATTCTCTCCGCTATTGGGTCGAGGAGTGCCATGTGGACGGCTTCCGGTTCGACCTTGCAAC
AGCCCTGGGGCGTGAGCGGGACAATTTCGAGCCGTCCTCGGTCTTCTTCGACACGGTGCGGCAGGATCCCGTCCTTTCGC
GCGTGAAGCTCATCGCGGAGCCTTGGGATCTCGGCCCGGACGGCTATCAGCTTGGCAATTTTCCTCCCGGCTGGGGAGAG
TGGAACGGCGCCTACCGCGACTGGGTGCGCAGCTTCTGGCGCGGCGACGAGCGGGTGGCGCCGGACCTTGCGAGCGGGCT
TCTGGGATCCGCCGCCATGTTCGACCGGCGCGGCCGCAGACCTTGGGCAAGCGTGAACTTCATCACCGCCCATGACGGTT
TCACGCTTCACGATCTTTACGCCTATAACGAGCGCCACAACGAAGCGAACCTGGAGGACAACCGCGACGGCCACAGCGAC
AACAGGAGCTGGAATTGCGGCGCCGAGGGGCCGACGGACGACGAGACCATCAAGGCGCTGAGGGCCAAGATGCGCCGCAA
TGCGATAGCGACGCTTCTCCTGTCGCAGGGGACTCCCATGGTGCTGATGGGCGATGAGGTTGGCCGCACGCAGAACGGGA
ATAACAACGCTTACTGCCAGGACAATGAGATAAACTGGCTGGCTTGGGAAAATCGCGATCCCGATGATGAAGCCTTTCTC
CAGTTCGTCCGGCGGCTCATTGCCTATCGCCGCGCCCATCCACGGCTTCGGCTCAAAAATTTCCTGCACGGCAATGTCGT
TGCGGGGAAATATCGCGACGTTTCGTGGCTGCGGCCGACCGGCGAGCCGATGACCGACGAGGACTGGAGCGATCCGGGCT
TCAAAAGCTTCGGCTTGATGCTCTGCGACGAGCGCGGCACCTGTATCCTGATCTTGATCAACGGCTTTCATGAGCCGTTG
GGTTTCAGTCTTCCCCGTGAATTGGGCGCTGCCGGCTGGAAGATGATCATGCGTACCGATACCGGCCAATGGCCGGTCTG
GGAGGCCATGCGTTCTCCGCACACGCTGGAACTCGCCGACCGTTCGATGGTTGTGCTGGAAGGCCGTGTACGATGA

Upstream 100 bases:

>100_bases
CCGACGAGCCGTGGCACGGCCGGCCTGCTTCAGCGATCCTGAGGCTGCCCCCGCTGGGCAGCGTCGTGCTTGTGGGTGAA
GCGTGACAGGAGACCCACAC

Downstream 100 bases:

>100_bases
CCCACACAACTTTCCAGACACGCTGGGGCGCTCAGCTCCAGGATGACGATACCGCTCTTTTCCGCCTCTGGGCGCCGGCG
GCGGACCAACTTCGCCTGCG

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MRVEIGLPYPLGATWDGSGVNVAVFSAHAEKIELCLFDAQGRREIRRIPLPEYTHEVWHGYFPDMRPGQVYGLRAYGPYD
PANGHRFNPNKLLIDPYAKQLLGDIRWHDACFGYRVGGARQDLAIDRRDSAFVMPKCVVTDTAATWGNDVRPHRPWEETI
IYEAHVKGMTALHPDVPEQFRGTFGGLADSRVIDYLVKLGITAIELLPVQAFFDDRYLVEKRLTNYWGYNTIGFFAPTPR
YISPRGGLHEFKRMVRKLHEAGIEVILDVVYNHTAEGNELGPTLSFRGLDNASYYILGDDPRYYYDTTGCGNTLNLKHPR
VLQMVMDSLRYWVEECHVDGFRFDLATALGRERDNFEPSSVFFDTVRQDPVLSRVKLIAEPWDLGPDGYQLGNFPPGWGE
WNGAYRDWVRSFWRGDERVAPDLASGLLGSAAMFDRRGRRPWASVNFITAHDGFTLHDLYAYNERHNEANLEDNRDGHSD
NRSWNCGAEGPTDDETIKALRAKMRRNAIATLLLSQGTPMVLMGDEVGRTQNGNNNAYCQDNEINWLAWENRDPDDEAFL
QFVRRLIAYRRAHPRLRLKNFLHGNVVAGKYRDVSWLRPTGEPMTDEDWSDPGFKSFGLMLCDERGTCILILINGFHEPL
GFSLPRELGAAGWKMIMRTDTGQWPVWEAMRSPHTLELADRSMVVLEGRVR

Sequences:

>Translated_691_residues
MRVEIGLPYPLGATWDGSGVNVAVFSAHAEKIELCLFDAQGRREIRRIPLPEYTHEVWHGYFPDMRPGQVYGLRAYGPYD
PANGHRFNPNKLLIDPYAKQLLGDIRWHDACFGYRVGGARQDLAIDRRDSAFVMPKCVVTDTAATWGNDVRPHRPWEETI
IYEAHVKGMTALHPDVPEQFRGTFGGLADSRVIDYLVKLGITAIELLPVQAFFDDRYLVEKRLTNYWGYNTIGFFAPTPR
YISPRGGLHEFKRMVRKLHEAGIEVILDVVYNHTAEGNELGPTLSFRGLDNASYYILGDDPRYYYDTTGCGNTLNLKHPR
VLQMVMDSLRYWVEECHVDGFRFDLATALGRERDNFEPSSVFFDTVRQDPVLSRVKLIAEPWDLGPDGYQLGNFPPGWGE
WNGAYRDWVRSFWRGDERVAPDLASGLLGSAAMFDRRGRRPWASVNFITAHDGFTLHDLYAYNERHNEANLEDNRDGHSD
NRSWNCGAEGPTDDETIKALRAKMRRNAIATLLLSQGTPMVLMGDEVGRTQNGNNNAYCQDNEINWLAWENRDPDDEAFL
QFVRRLIAYRRAHPRLRLKNFLHGNVVAGKYRDVSWLRPTGEPMTDEDWSDPGFKSFGLMLCDERGTCILILINGFHEPL
GFSLPRELGAAGWKMIMRTDTGQWPVWEAMRSPHTLELADRSMVVLEGRVR
>Mature_691_residues
MRVEIGLPYPLGATWDGSGVNVAVFSAHAEKIELCLFDAQGRREIRRIPLPEYTHEVWHGYFPDMRPGQVYGLRAYGPYD
PANGHRFNPNKLLIDPYAKQLLGDIRWHDACFGYRVGGARQDLAIDRRDSAFVMPKCVVTDTAATWGNDVRPHRPWEETI
IYEAHVKGMTALHPDVPEQFRGTFGGLADSRVIDYLVKLGITAIELLPVQAFFDDRYLVEKRLTNYWGYNTIGFFAPTPR
YISPRGGLHEFKRMVRKLHEAGIEVILDVVYNHTAEGNELGPTLSFRGLDNASYYILGDDPRYYYDTTGCGNTLNLKHPR
VLQMVMDSLRYWVEECHVDGFRFDLATALGRERDNFEPSSVFFDTVRQDPVLSRVKLIAEPWDLGPDGYQLGNFPPGWGE
WNGAYRDWVRSFWRGDERVAPDLASGLLGSAAMFDRRGRRPWASVNFITAHDGFTLHDLYAYNERHNEANLEDNRDGHSD
NRSWNCGAEGPTDDETIKALRAKMRRNAIATLLLSQGTPMVLMGDEVGRTQNGNNNAYCQDNEINWLAWENRDPDDEAFL
QFVRRLIAYRRAHPRLRLKNFLHGNVVAGKYRDVSWLRPTGEPMTDEDWSDPGFKSFGLMLCDERGTCILILINGFHEPL
GFSLPRELGAAGWKMIMRTDTGQWPVWEAMRSPHTLELADRSMVVLEGRVR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=656, Percent_Identity=46.3414634146341, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17554896, Length=201, Percent_Identity=29.8507462686567, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI32564391, Length=201, Percent_Identity=29.8507462686567, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28573410, Length=198, Percent_Identity=26.7676767676768, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78987; Mature: 78987

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVEIGLPYPLGATWDGSGVNVAVFSAHAEKIELCLFDAQGRREIRRIPLPEYTHEVWHG
CEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHC
YFPDMRPGQVYGLRAYGPYDPANGHRFNPNKLLIDPYAKQLLGDIRWHDACFGYRVGGAR
CCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHHHHHHHHHHCEECCCCC
QDLAIDRRDSAFVMPKCVVTDTAATWGNDVRPHRPWEETIIYEAHVKGMTALHPDVPEQF
CCCEECCCCCEEECCEEEEECCCHHCCCCCCCCCCHHHCEEEEECCCCCEECCCCCCHHH
RGTFGGLADSRVIDYLVKLGITAIELLPVQAFFDDRYLVEKRLTNYWGYNTIGFFAPTPR
HHHHCCCHHHHHHHHHHHHCCHHEEEEEHHHHHCCHHHHHHHHHHHCCCCCEEECCCCCC
YISPRGGLHEFKRMVRKLHEAGIEVILDVVYNHTAEGNELGPTLSFRGLDNASYYILGDD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCC
PRYYYDTTGCGNTLNLKHPRVLQMVMDSLRYWVEECHVDGFRFDLATALGRERDNFEPSS
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
VFFDTVRQDPVLSRVKLIAEPWDLGPDGYQLGNFPPGWGEWNGAYRDWVRSFWRGDERVA
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PDLASGLLGSAAMFDRRGRRPWASVNFITAHDGFTLHDLYAYNERHNEANLEDNRDGHSD
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCCCC
NRSWNCGAEGPTDDETIKALRAKMRRNAIATLLLSQGTPMVLMGDEVGRTQNGNNNAYCQ
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEE
DNEINWLAWENRDPDDEAFLQFVRRLIAYRRAHPRLRLKNFLHGNVVAGKYRDVSWLRPT
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCEEECCC
GEPMTDEDWSDPGFKSFGLMLCDERGTCILILINGFHEPLGFSLPRELGAAGWKMIMRTD
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHCCCCEEEEEECC
TGQWPVWEAMRSPHTLELADRSMVVLEGRVR
CCCCCHHHHHCCCCEEEECCCEEEEEECCCC
>Mature Secondary Structure
MRVEIGLPYPLGATWDGSGVNVAVFSAHAEKIELCLFDAQGRREIRRIPLPEYTHEVWHG
CEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHC
YFPDMRPGQVYGLRAYGPYDPANGHRFNPNKLLIDPYAKQLLGDIRWHDACFGYRVGGAR
CCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHHHHHHHHHHCEECCCCC
QDLAIDRRDSAFVMPKCVVTDTAATWGNDVRPHRPWEETIIYEAHVKGMTALHPDVPEQF
CCCEECCCCCEEECCEEEEECCCHHCCCCCCCCCCHHHCEEEEECCCCCEECCCCCCHHH
RGTFGGLADSRVIDYLVKLGITAIELLPVQAFFDDRYLVEKRLTNYWGYNTIGFFAPTPR
HHHHCCCHHHHHHHHHHHHCCHHEEEEEHHHHHCCHHHHHHHHHHHCCCCCEEECCCCCC
YISPRGGLHEFKRMVRKLHEAGIEVILDVVYNHTAEGNELGPTLSFRGLDNASYYILGDD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCC
PRYYYDTTGCGNTLNLKHPRVLQMVMDSLRYWVEECHVDGFRFDLATALGRERDNFEPSS
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
VFFDTVRQDPVLSRVKLIAEPWDLGPDGYQLGNFPPGWGEWNGAYRDWVRSFWRGDERVA
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PDLASGLLGSAAMFDRRGRRPWASVNFITAHDGFTLHDLYAYNERHNEANLEDNRDGHSD
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCCCC
NRSWNCGAEGPTDDETIKALRAKMRRNAIATLLLSQGTPMVLMGDEVGRTQNGNNNAYCQ
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEE
DNEINWLAWENRDPDDEAFLQFVRRLIAYRRAHPRLRLKNFLHGNVVAGKYRDVSWLRPT
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCEEECCC
GEPMTDEDWSDPGFKSFGLMLCDERGTCILILINGFHEPLGFSLPRELGAAGWKMIMRTD
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHCCCCEEEEEECC
TGQWPVWEAMRSPHTLELADRSMVVLEGRVR
CCCCCHHHHHCCCCEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]