Click here to switch to the map view.

The map label for this gene is proW [H]

Identifier: 110634871

GI number: 110634871

Start: 2709127

End: 2710071

Strand: Direct

Name: proW [H]

Synonym: Meso_2530

Alternate gene names: 110634871

Gene position: 2709127-2710071 (Clockwise)

Preceding gene: 110634870

Following gene: 110634872

Centisome position: 61.4

GC content: 61.8

Gene sequence:

>945_bases
ATGCTTAATGCCGACGATCTTGCTGTCTTTCCGGTGGATGTCTGGATCAGCGATGCGGTCCAGTGGGTCGCGCTCAACCT
GCGTCCGCTTTTCCTGGCCATCAAGTGGCCGATCGAAAGCCTTCTGACGTTAATCGACGGCGCCATGCACGATGCCCCCT
TCCCGCTCGTGGTGATCCTATTCACGGCACTGGCCTATATGCTCGCCAGCCGCAAGATTGCGATCTTCACCGCCATAGTA
CTGGTCCTTGTTGCGGCGATGGGAGTGTGGGATCCGGCGATGACCACGTTGTCGCTGATCACGACGGCGATCTTCTTCTG
CGCGTTGATCGGCCTGCCGGTCGGTATCTGGTGTGCGCGCAGCGACAGGGCCTGGAGCATAGTCCGGCCGATACTCGACA
TTATGCAGACGACACCAACCTTCGTCTATCTCGTGCCGGTGGTGATGCTGTTCGGCGTGGGCACTGTGCCGGGCCAGGTG
GCCGTGGTCGTGGCTGCGGTGCCGCCGCTTATACGCTTTACCAATCTCGGCATCCGCATGGTCGATCGTGAGATCGTCGA
AGCGGGTATTGCCTTTGGCGCGAGCAGCCGGCAATTGCTGTGGGAAATCCAAATCCCCATGGCGATCCCGACGATCCTGG
GCGGTCTTAACCAGACGGTGCTGACCGCCATGGTCATGTCCGTGGTCGTCGCCATGATTGGCGCCGAAGGGCTGGGGCTG
GTCGTGCTGCAGGGCATAGGACGGCTCGATGTCGGCCGTGCCGTTATCGGCGGGATTGCCATTGTTCTGCTGGCCATGAT
GCTCGACAGGATCACCCAGAAGATGGCTGAACCCAAGCGCGCGACGGCGCGGTCGCGCTGGCTTGCCGCCCTCAACCTCG
ACCGGCTTCTGTCGCGCAACGGCAGCAGCGAGACGGTCGCATCCCAATCCGGCACCAACGCCTGA

Upstream 100 bases:

>100_bases
GAGGGAACCTTTCTGGGGGTGCTTTCGCCCGCCAGCATCCTTACCGCCATTGCCGCGTCATCTAATGCCGGCCAAGAACG
CCACCTTGAGGAAAAGCGCC

Downstream 100 bases:

>100_bases
TGAAGCGGGCTGGGCAGGGCCGGATGCCCGGAACGCTCCGTACGAGATGCGGCCGTCCGTAACGGCTGCATCGCTTCCCG
AGCCATGAAGATAATCGTCC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLNADDLAVFPVDVWISDAVQWVALNLRPLFLAIKWPIESLLTLIDGAMHDAPFPLVVILFTALAYMLASRKIAIFTAIV
LVLVAAMGVWDPAMTTLSLITTAIFFCALIGLPVGIWCARSDRAWSIVRPILDIMQTTPTFVYLVPVVMLFGVGTVPGQV
AVVVAAVPPLIRFTNLGIRMVDREIVEAGIAFGASSRQLLWEIQIPMAIPTILGGLNQTVLTAMVMSVVVAMIGAEGLGL
VVLQGIGRLDVGRAVIGGIAIVLLAMMLDRITQKMAEPKRATARSRWLAALNLDRLLSRNGSSETVASQSGTNA

Sequences:

>Translated_314_residues
MLNADDLAVFPVDVWISDAVQWVALNLRPLFLAIKWPIESLLTLIDGAMHDAPFPLVVILFTALAYMLASRKIAIFTAIV
LVLVAAMGVWDPAMTTLSLITTAIFFCALIGLPVGIWCARSDRAWSIVRPILDIMQTTPTFVYLVPVVMLFGVGTVPGQV
AVVVAAVPPLIRFTNLGIRMVDREIVEAGIAFGASSRQLLWEIQIPMAIPTILGGLNQTVLTAMVMSVVVAMIGAEGLGL
VVLQGIGRLDVGRAVIGGIAIVLLAMMLDRITQKMAEPKRATARSRWLAALNLDRLLSRNGSSETVASQSGTNA
>Mature_314_residues
MLNADDLAVFPVDVWISDAVQWVALNLRPLFLAIKWPIESLLTLIDGAMHDAPFPLVVILFTALAYMLASRKIAIFTAIV
LVLVAAMGVWDPAMTTLSLITTAIFFCALIGLPVGIWCARSDRAWSIVRPILDIMQTTPTFVYLVPVVMLFGVGTVPGQV
AVVVAAVPPLIRFTNLGIRMVDREIVEAGIAFGASSRQLLWEIQIPMAIPTILGGLNQTVLTAMVMSVVVAMIGAEGLGL
VVLQGIGRLDVGRAVIGGIAIVLLAMMLDRITQKMAEPKRATARSRWLAALNLDRLLSRNGSSETVASQSGTNA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=287, Percent_Identity=44.9477351916376, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788451, Length=198, Percent_Identity=29.2929292929293, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788449, Length=194, Percent_Identity=23.7113402061856, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=30.3225806451613, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33682; Mature: 33682

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADDLAVFPVDVWISDAVQWVALNLRPLFLAIKWPIESLLTLIDGAMHDAPFPLVVIL
CCCCCCEEEEEHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
FTALAYMLASRKIAIFTAIVLVLVAAMGVWDPAMTTLSLITTAIFFCALIGLPVGIWCAR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECC
SDRAWSIVRPILDIMQTTPTFVYLVPVVMLFGVGTVPGQVAVVVAAVPPLIRFTNLGIRM
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VDREIVEAGIAFGASSRQLLWEIQIPMAIPTILGGLNQTVLTAMVMSVVVAMIGAEGLGL
HHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
VVLQGIGRLDVGRAVIGGIAIVLLAMMLDRITQKMAEPKRATARSRWLAALNLDRLLSRN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GSSETVASQSGTNA
CCCCHHHCCCCCCC
>Mature Secondary Structure
MLNADDLAVFPVDVWISDAVQWVALNLRPLFLAIKWPIESLLTLIDGAMHDAPFPLVVIL
CCCCCCEEEEEHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
FTALAYMLASRKIAIFTAIVLVLVAAMGVWDPAMTTLSLITTAIFFCALIGLPVGIWCAR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECC
SDRAWSIVRPILDIMQTTPTFVYLVPVVMLFGVGTVPGQVAVVVAAVPPLIRFTNLGIRM
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VDREIVEAGIAFGASSRQLLWEIQIPMAIPTILGGLNQTVLTAMVMSVVVAMIGAEGLGL
HHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
VVLQGIGRLDVGRAVIGGIAIVLLAMMLDRITQKMAEPKRATARSRWLAALNLDRLLSRN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GSSETVASQSGTNA
CCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]