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The map label for this gene is asnB [H]

Identifier: 110634810

GI number: 110634810

Start: 2646868

End: 2648643

Strand: Reverse

Name: asnB [H]

Synonym: Meso_2469

Alternate gene names: 110634810

Gene position: 2648643-2646868 (Counterclockwise)

Preceding gene: 110634820

Following gene: 110634809

Centisome position: 60.03

GC content: 63.18

Gene sequence:

>1776_bases
ATGTGCGGCATTTGCGGTGAAGTGAGATTTGACGGCCGGACTCCGTCCGTATCGGCCATATCCGGAATGATGGAAGCGCT
GGCTCCGAGAGGGCCCGACGGCGCGGGCATCGTGTCCCACGGCAATGCGGGATTCGGCCACCGGCGGCTCAGGATCATCG
ATCTTTCGGAAAAGGCGCAGCAGCCGATGGTGGACTCCGCGTTGGGCCTGACCATCGCCTTCAACGGCTGCATCTACAAT
TACCCCGAGCTGCGCGAGGCGCTCGAGGCAAAGGGCTATAGCTTTTTCTCGCACGGCGACACGGAGGTCATCCTCAAGGC
GTGGCATGCCTGGGGTGAAACCTGCGTGGAGCGCTTCCATGGCATGTTCGCTTTCGTGATCCAGGAGCGCGACACCGGCC
GTATCGTGATGGCGCGGGACCGCTTCGGCATCAAGCCGCTCTACCTCGCCGAGAAGAGCGGCGTGCTCCGCTTCGCCTCC
TCGCTCCCGGCGCTCGTGAAAGCCGGGGACGTGGATACCTCCATCGACCGGGTGGCGCTGCATCACTACATGACCTTCCA
CGCGGTCGTTCCGCCGCCCCGCACTATCCTCAATGGTGTGCGCAAGCTGCCGCCCGCCACGATCCGCGTCATCGAGGCCG
ACGGCCGCTCGACGGAACGGCGCTACTGGGATCCGCCCTATCGCCGCGACCCGGCCGATGCCGGCGTTTCGGCAGAAGAG
TGGCGCGACCGTGTGCACGAGGCGCTACGGCTTGCCGTGAAGCGGCGCATGGTGGCTGATGTACCCGTGGGCGTGCTGCT
TTCAGGCGGTGTGGATTCGAGCCTCATTGTCGGCCTTCTGGCCGAGGCCGGCCAGACCGGGCTCATGAGCTTTTCCGTCG
GTTTCGAGGAAGCGAATGGCGAGAAAGGGGACGAGTTCGTCTATTCCGACTTGATCGCGAAGGAGTTCGGCACCGATCAT
CACAAGATATTCGTGCCTTCCGACCGGCTGATGGATGCGCTCCCCGGCACCTTCGCCGCCATGTCCGAGCCGATGGTGTC
CTACGACAATGTGGGCTTCTATCTGCTCTCGCAGGAGGTTTCCAAGCACATCAAGGTGGTGCAGTCGGGGCAGGGAGCTG
ACGAGGTGTTCGGCGGCTATCACTGGTATCCGCCGCTTGCCCGCTCCAACGACGTGGTGGCAGACTACGCAAAGGTCTTC
TTTGACCGCTCGCACGAGACGCTGAAGCGTCAGCTCGGAGCGAACTGGATCAATGGCGAAGACTACAGCCGCGGCCTTGT
CGCCGCGGAGCTGATGCGGCCGGGTGCGGACTCGCCTGTCGATCGTGCTTTGAGGCTCGATGCCAATGTGATGCTGGTGG
ACGATCCGGTGAAGCGGGTCGACAACATGACGATGGCCTGGGGGCTGGAGGCTCGTGTTCCCTTCCTCGATCACGAGCTG
GTTGAGCTCGCCGCGCGCATTCCGCCCGAAGAAAAGCTGAAAGACGGCGGCAAAGGCGTGCTCAAGGATGTGGCCCGGCT
TGTCGTGCCGCATGAGGTCATCGATCGACCGAAGGGTTATTTCCCGGTGCCGCAGCTCAAATATGTGGCCGGTCCCTATC
TTGAGATGGTGCGTGACGCGTTGGGCTCTCAACGCGCTCGCGAGCGCGGGCTGTTTCGCCAGGATTATCTCGATCGGCTT
TTCGCTCAGCCCGCCGACCACATCACGCCGCTGCGCGGTTCCGAGCTCTGGCAGGCCGGACTTCTGGAATTGTGGCTTCA
GACCCATGGGATCTAG

Upstream 100 bases:

>100_bases
GTTCATTTGACTTCAAATCATTTGAACCCTAACTATAGGTCCAGGCAAGCCACTCGACAAGAGATCAATCAACAAAGACA
ATGGAGGTAAACGCCCGCCG

Downstream 100 bases:

>100_bases
CGAGATGACGAAAACTTCCGGCGCGAACAGGAAAGGGAGTGCAGGCCGGAGCGACAAGGCGCTCGACCACCGGCTGAGAA
GGCTGCGCGCCCATGCCAAG

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MCGICGEVRFDGRTPSVSAISGMMEALAPRGPDGAGIVSHGNAGFGHRRLRIIDLSEKAQQPMVDSALGLTIAFNGCIYN
YPELREALEAKGYSFFSHGDTEVILKAWHAWGETCVERFHGMFAFVIQERDTGRIVMARDRFGIKPLYLAEKSGVLRFAS
SLPALVKAGDVDTSIDRVALHHYMTFHAVVPPPRTILNGVRKLPPATIRVIEADGRSTERRYWDPPYRRDPADAGVSAEE
WRDRVHEALRLAVKRRMVADVPVGVLLSGGVDSSLIVGLLAEAGQTGLMSFSVGFEEANGEKGDEFVYSDLIAKEFGTDH
HKIFVPSDRLMDALPGTFAAMSEPMVSYDNVGFYLLSQEVSKHIKVVQSGQGADEVFGGYHWYPPLARSNDVVADYAKVF
FDRSHETLKRQLGANWINGEDYSRGLVAAELMRPGADSPVDRALRLDANVMLVDDPVKRVDNMTMAWGLEARVPFLDHEL
VELAARIPPEEKLKDGGKGVLKDVARLVVPHEVIDRPKGYFPVPQLKYVAGPYLEMVRDALGSQRARERGLFRQDYLDRL
FAQPADHITPLRGSELWQAGLLELWLQTHGI

Sequences:

>Translated_591_residues
MCGICGEVRFDGRTPSVSAISGMMEALAPRGPDGAGIVSHGNAGFGHRRLRIIDLSEKAQQPMVDSALGLTIAFNGCIYN
YPELREALEAKGYSFFSHGDTEVILKAWHAWGETCVERFHGMFAFVIQERDTGRIVMARDRFGIKPLYLAEKSGVLRFAS
SLPALVKAGDVDTSIDRVALHHYMTFHAVVPPPRTILNGVRKLPPATIRVIEADGRSTERRYWDPPYRRDPADAGVSAEE
WRDRVHEALRLAVKRRMVADVPVGVLLSGGVDSSLIVGLLAEAGQTGLMSFSVGFEEANGEKGDEFVYSDLIAKEFGTDH
HKIFVPSDRLMDALPGTFAAMSEPMVSYDNVGFYLLSQEVSKHIKVVQSGQGADEVFGGYHWYPPLARSNDVVADYAKVF
FDRSHETLKRQLGANWINGEDYSRGLVAAELMRPGADSPVDRALRLDANVMLVDDPVKRVDNMTMAWGLEARVPFLDHEL
VELAARIPPEEKLKDGGKGVLKDVARLVVPHEVIDRPKGYFPVPQLKYVAGPYLEMVRDALGSQRARERGLFRQDYLDRL
FAQPADHITPLRGSELWQAGLLELWLQTHGI
>Mature_591_residues
MCGICGEVRFDGRTPSVSAISGMMEALAPRGPDGAGIVSHGNAGFGHRRLRIIDLSEKAQQPMVDSALGLTIAFNGCIYN
YPELREALEAKGYSFFSHGDTEVILKAWHAWGETCVERFHGMFAFVIQERDTGRIVMARDRFGIKPLYLAEKSGVLRFAS
SLPALVKAGDVDTSIDRVALHHYMTFHAVVPPPRTILNGVRKLPPATIRVIEADGRSTERRYWDPPYRRDPADAGVSAEE
WRDRVHEALRLAVKRRMVADVPVGVLLSGGVDSSLIVGLLAEAGQTGLMSFSVGFEEANGEKGDEFVYSDLIAKEFGTDH
HKIFVPSDRLMDALPGTFAAMSEPMVSYDNVGFYLLSQEVSKHIKVVQSGQGADEVFGGYHWYPPLARSNDVVADYAKVF
FDRSHETLKRQLGANWINGEDYSRGLVAAELMRPGADSPVDRALRLDANVMLVDDPVKRVDNMTMAWGLEARVPFLDHEL
VELAARIPPEEKLKDGGKGVLKDVARLVVPHEVIDRPKGYFPVPQLKYVAGPYLEMVRDALGSQRARERGLFRQDYLDRL
FAQPADHITPLRGSELWQAGLLELWLQTHGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=567, Percent_Identity=28.9241622574956, Blast_Score=165, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25147557, Length=550, Percent_Identity=27.6363636363636, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=550, Percent_Identity=27.6363636363636, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=603, Percent_Identity=24.3781094527363, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325403, Length=576, Percent_Identity=26.7361111111111, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=565, Percent_Identity=25.4867256637168, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553209, Length=519, Percent_Identity=27.3603082851638, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65548; Mature: 65548

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGEVRFDGRTPSVSAISGMMEALAPRGPDGAGIVSHGNAGFGHRRLRIIDLSEKAQ
CCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEECHHHHC
QPMVDSALGLTIAFNGCIYNYPELREALEAKGYSFFSHGDTEVILKAWHAWGETCVERFH
CCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHC
GMFAFVIQERDTGRIVMARDRFGIKPLYLAEKSGVLRFASSLPALVKAGDVDTSIDRVAL
CCEEEEEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCHHHCCCCCCCHHHHHHH
HHYMTFHAVVPPPRTILNGVRKLPPATIRVIEADGRSTERRYWDPPYRRDPADAGVSAEE
HHHHHEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
WRDRVHEALRLAVKRRMVADVPVGVLLSGGVDSSLIVGLLAEAGQTGLMSFSVGFEEANG
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCC
EKGDEFVYSDLIAKEFGTDHHKIFVPSDRLMDALPGTFAAMSEPMVSYDNVGFYLLSQEV
CCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHCCCHHHHHCCCCCCCCCCCEEEEHHHH
SKHIKVVQSGQGADEVFGGYHWYPPLARSNDVVADYAKVFFDRSHETLKRQLGANWINGE
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
DYSRGLVAAELMRPGADSPVDRALRLDANVMLVDDPVKRVDNMTMAWGLEARVPFLDHEL
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCHHHHHCCCEEEECCCCCCCCHHHHH
VELAARIPPEEKLKDGGKGVLKDVARLVVPHEVIDRPKGYFPVPQLKYVAGPYLEMVRDA
HHHHHHCCCHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHCCHHHHHHHHH
LGSQRARERGLFRQDYLDRLFAQPADHITPLRGSELWQAGLLELWLQTHGI
HHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGICGEVRFDGRTPSVSAISGMMEALAPRGPDGAGIVSHGNAGFGHRRLRIIDLSEKAQ
CCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEECHHHHC
QPMVDSALGLTIAFNGCIYNYPELREALEAKGYSFFSHGDTEVILKAWHAWGETCVERFH
CCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHC
GMFAFVIQERDTGRIVMARDRFGIKPLYLAEKSGVLRFASSLPALVKAGDVDTSIDRVAL
CCEEEEEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCHHHCCCCCCCHHHHHHH
HHYMTFHAVVPPPRTILNGVRKLPPATIRVIEADGRSTERRYWDPPYRRDPADAGVSAEE
HHHHHEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
WRDRVHEALRLAVKRRMVADVPVGVLLSGGVDSSLIVGLLAEAGQTGLMSFSVGFEEANG
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCC
EKGDEFVYSDLIAKEFGTDHHKIFVPSDRLMDALPGTFAAMSEPMVSYDNVGFYLLSQEV
CCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHCCCHHHHHCCCCCCCCCCCEEEEHHHH
SKHIKVVQSGQGADEVFGGYHWYPPLARSNDVVADYAKVFFDRSHETLKRQLGANWINGE
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
DYSRGLVAAELMRPGADSPVDRALRLDANVMLVDDPVKRVDNMTMAWGLEARVPFLDHEL
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCHHHHHCCCEEEECCCCCCCCHHHHH
VELAARIPPEEKLKDGGKGVLKDVARLVVPHEVIDRPKGYFPVPQLKYVAGPYLEMVRDA
HHHHHHCCCHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHCCHHHHHHHHH
LGSQRARERGLFRQDYLDRLFAQPADHITPLRGSELWQAGLLELWLQTHGI
HHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]