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The map label for this gene is ycdT [H]

Identifier: 110634808

GI number: 110634808

Start: 2644022

End: 2644741

Strand: Reverse

Name: ycdT [H]

Synonym: Meso_2467

Alternate gene names: 110634808

Gene position: 2644741-2644022 (Counterclockwise)

Preceding gene: 110634809

Following gene: 110634806

Centisome position: 59.94

GC content: 54.72

Gene sequence:

>720_bases
ATGTTTTGGCAATGTAGCCTGCTGGCTATCATCGCGCTCCTCGGAGCGGAAATTCTCACGCTCATCTTTTATTCAATCTT
TTTCAGCGACCGCCTTTTTCTCGACCTGCTGCTGAGCGGCATTATCGTTTTGGTTGTCGCGTATCCACTCTCCTACATCT
TCCTCACCCAAAGCGTGCGACTGATGCGCATGGCTGAGGAAATCGAACGGCGGTCCCGAATTGACGATCTGAGCCAGGTG
CTCAACCGCAAAAGCTTTCTCGATGCTGCTGACGATCTTCTTGCAGCCTCTTCTTCACCATCCGGTGCAGGAACATTCCT
CTATCTCGACATCGACTGGTTCAAGACAATCAATGACGAGAGAGGACATGCGATGGGCGACGAGGTCATTCGGCGAATGG
GTGCACTACTTCAATCCTGCGTTCGGCATGGAGACATCGTAGGCCGGCTCGGCGGTGAGGAATTCGGGATCTTCCTTCCT
GCCGGTGACGATGAGGATGCGGCGGCTGTTTATAGCCGCATTGCCCAGCGTGCCAAGGAGATCGGACAGGAGCTTGGCCT
CGACGATAGGCACGTCTCGCTGAGTGCGGGTATGATTTCACATCGGCCCGGCCAGGGCTTTAACGAGTTGATGAAGCTTG
CCGATAAAAGGCTCTATCGGGCGAAGTCGAAGGGACGAAACCGGCTCATCTCAAAAGACGGGCCAGGGATAGCCGCATAG

Upstream 100 bases:

>100_bases
CGCAGGAATAACTTTCTTCACTAAGCTCTGTTATTCTGCTTGGTGGGTTGGGGCAGGTAACATGGTCAGTGCGTGGGCTC
GTAGGTTTTGGCGATTGCCG

Downstream 100 bases:

>100_bases
GGAAAGCGGAAATAGCTGCCGATATCTGCTTGCCTCTCGTGCCACTCTCGCTTTTTCAAACACATGAAAGAATTCAAGCG
TTGGTTCCCAGATGCCGCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MFWQCSLLAIIALLGAEILTLIFYSIFFSDRLFLDLLLSGIIVLVVAYPLSYIFLTQSVRLMRMAEEIERRSRIDDLSQV
LNRKSFLDAADDLLAASSSPSGAGTFLYLDIDWFKTINDERGHAMGDEVIRRMGALLQSCVRHGDIVGRLGGEEFGIFLP
AGDDEDAAAVYSRIAQRAKEIGQELGLDDRHVSLSAGMISHRPGQGFNELMKLADKRLYRAKSKGRNRLISKDGPGIAA

Sequences:

>Translated_239_residues
MFWQCSLLAIIALLGAEILTLIFYSIFFSDRLFLDLLLSGIIVLVVAYPLSYIFLTQSVRLMRMAEEIERRSRIDDLSQV
LNRKSFLDAADDLLAASSSPSGAGTFLYLDIDWFKTINDERGHAMGDEVIRRMGALLQSCVRHGDIVGRLGGEEFGIFLP
AGDDEDAAAVYSRIAQRAKEIGQELGLDDRHVSLSAGMISHRPGQGFNELMKLADKRLYRAKSKGRNRLISKDGPGIAA
>Mature_239_residues
MFWQCSLLAIIALLGAEILTLIFYSIFFSDRLFLDLLLSGIIVLVVAYPLSYIFLTQSVRLMRMAEEIERRSRIDDLSQV
LNRKSFLDAADDLLAASSSPSGAGTFLYLDIDWFKTINDERGHAMGDEVIRRMGALLQSCVRHGDIVGRLGGEEFGIFLP
AGDDEDAAAVYSRIAQRAKEIGQELGLDDRHVSLSAGMISHRPGQGFNELMKLADKRLYRAKSKGRNRLISKDGPGIAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=30.8108108108108, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=33.7278106508876, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87082007, Length=207, Percent_Identity=31.8840579710145, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=31.1475409836066, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=190, Percent_Identity=30, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=31.7647058823529, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=28.7234042553192, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=32.3353293413174, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=185, Percent_Identity=26.4864864864865, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=32.2784810126582, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 26501; Mature: 26501

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWQCSLLAIIALLGAEILTLIFYSIFFSDRLFLDLLLSGIIVLVVAYPLSYIFLTQSVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMRMAEEIERRSRIDDLSQVLNRKSFLDAADDLLAASSSPSGAGTFLYLDIDWFKTINDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCC
RGHAMGDEVIRRMGALLQSCVRHGDIVGRLGGEEFGIFLPAGDDEDAAAVYSRIAQRAKE
CCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
IGQELGLDDRHVSLSAGMISHRPGQGFNELMKLADKRLYRAKSKGRNRLISKDGPGIAA
HHHHCCCCCCCEEHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFWQCSLLAIIALLGAEILTLIFYSIFFSDRLFLDLLLSGIIVLVVAYPLSYIFLTQSVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMRMAEEIERRSRIDDLSQVLNRKSFLDAADDLLAASSSPSGAGTFLYLDIDWFKTINDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCC
RGHAMGDEVIRRMGALLQSCVRHGDIVGRLGGEEFGIFLPAGDDEDAAAVYSRIAQRAKE
CCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
IGQELGLDDRHVSLSAGMISHRPGQGFNELMKLADKRLYRAKSKGRNRLISKDGPGIAA
HHHHCCCCCCCEEHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]