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The map label for this gene is gsiB [H]

Identifier: 110634750

GI number: 110634750

Start: 2566071

End: 2567588

Strand: Reverse

Name: gsiB [H]

Synonym: Meso_2408

Alternate gene names: 110634750

Gene position: 2567588-2566071 (Counterclockwise)

Preceding gene: 110634751

Following gene: 110634749

Centisome position: 58.19

GC content: 62.45

Gene sequence:

>1518_bases
ATGACTTTTTCCTTCAAGCGCAGCCTGATGGCGGCCGCAGTGGGTGTCAGCGCGGTGACGCTGATCGCCGGCAGCGGCCT
GGCACAGGACAACTCGACCGTCGTCGTTGCGCAGGCCGTGGACGCCTATACGATGGATCCCGCCAAGCATTCCAACTTCC
CGACCGCGAACATCCTGTTTCAGATCTATGACGGCCTGATCGCGGAGGGAGAGAATGGCGAGCTCGTGCCGGCGCTGGCC
GAATCCTGGAGCAACCCGGACGAACTGACCTGGCGCTTCAAGCTGCGGGAAGGCGTCACCTTCCATAATGGCGAGCCTTT
CAATGCCGAGGCGGTGAAGCTCAGCTTCGAGCGCGCGCTCGATCCCGAGTTCAAGGCCCCTTATTTCTCGCGCATCTCGC
ACATCAAGGCCGTGGAGGTCGTCGACGAGTATACGGTCGACTTCAAGACGGAGGAGCCGTTCCCGACGATGCTGCTGTCG
CTGTACGAGGCGTCTTTCCCCGCCCTGATCGTGCCGCCGGCATATGCGAAGGAAAACGACGGCGCATCCATCGCCGCCCA
TCCGGTCGGCACCGGCCCCTATAAGTTCGTCGAATGGCTCAAAGACGAGCGCGTCGTGCTCGAAGCCAATCCCGATTATT
GGGGCGGCAAGCCGGCGATCGACAAGGTCGTCTTCCGTCCGATTGCCGAGACTCGCACCCGCATCGCCGAACTCACGAGC
GGCGGTGCGGATATCATTGTCGACGTGCCGCCCGAAGACATCGCCGCGCTCGACGGCGGCGACACCAAGGTCAGCACCAT
CGCCAGTGATTCCCTCTATTTCCTCGCCTTCGACACGACACGCGACACCCCGCTCAAGGACAAGCGCGTCCGGCAGGCGA
TCAACTACGCCGTCGATGTCGACGCCATCCAGGCTGCGCTGCTGAACGGCATGGGCACGCGCATCGCGCTGACCTTGCCC
ACGAACGCCTTCGCCTATGATCAGGCCTGGAAACCCTATCCCTATGACCCCGAAAAGGCCAAGCAGCTTCTGGCGGAAGC
CGGGTATCCGGAGGGCTTTGAGATTCCCTTCATGTCGCGCAAGGGCCGCTACATGAAGGACAGCGAGATTATCGAGGCAA
CAGCCGGTTTCCTCGCCCGCGTCGGCATCAAGGCCAACATCCAGTATCTGGAGCCGGGCGTGTGGGCGCAGGTTTCGGAA
CGCAAGGGGCGCGAAGGCATCACCTTCCCCGGCTGGGCGGGCCGCGATCCACAGCTTGTCTGGGCGCCTCTGCTGATCAC
CGGCCAGTACCAGAGCTATTTCAGCAATCCGGAGCTGGATGAACTGCTCAAGGCCGGCGCCTCGATCATCGATCCTGAGG
AGCGCAAGGCGAACTACGCGAAGGCAGCCGAAATCATCAAGGAAGAGGCGCCGCACCTGCCGATGATCCAGCCGCCGCTC
ATCTACGCCATTGATGCGAAACTGAACTGGACCCCGCGCAGCGACGGCATCATCGACCTGCGCAAGGCGCATTTCTAG

Upstream 100 bases:

>100_bases
CGTGCTTCCGCGAAGCGCGACCAAAGGGGAGACGACCGAGGAGTGACAGAATCATCAACCGCAAGGGCCGGTACTGGCCA
TTATAAAAGAGGGTAACGAA

Downstream 100 bases:

>100_bases
GCTTTCGCACCGACATCCACCGGCCGGTATCGATCCGGCCGGTGAGCATCCGTACTCGTGTCTGCCATGGAGTGGAGCAG
GCGTTGGCCGATTTCCTGAT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTFSFKRSLMAAAVGVSAVTLIAGSGLAQDNSTVVVAQAVDAYTMDPAKHSNFPTANILFQIYDGLIAEGENGELVPALA
ESWSNPDELTWRFKLREGVTFHNGEPFNAEAVKLSFERALDPEFKAPYFSRISHIKAVEVVDEYTVDFKTEEPFPTMLLS
LYEASFPALIVPPAYAKENDGASIAAHPVGTGPYKFVEWLKDERVVLEANPDYWGGKPAIDKVVFRPIAETRTRIAELTS
GGADIIVDVPPEDIAALDGGDTKVSTIASDSLYFLAFDTTRDTPLKDKRVRQAINYAVDVDAIQAALLNGMGTRIALTLP
TNAFAYDQAWKPYPYDPEKAKQLLAEAGYPEGFEIPFMSRKGRYMKDSEIIEATAGFLARVGIKANIQYLEPGVWAQVSE
RKGREGITFPGWAGRDPQLVWAPLLITGQYQSYFSNPELDELLKAGASIIDPEERKANYAKAAEIIKEEAPHLPMIQPPL
IYAIDAKLNWTPRSDGIIDLRKAHF

Sequences:

>Translated_505_residues
MTFSFKRSLMAAAVGVSAVTLIAGSGLAQDNSTVVVAQAVDAYTMDPAKHSNFPTANILFQIYDGLIAEGENGELVPALA
ESWSNPDELTWRFKLREGVTFHNGEPFNAEAVKLSFERALDPEFKAPYFSRISHIKAVEVVDEYTVDFKTEEPFPTMLLS
LYEASFPALIVPPAYAKENDGASIAAHPVGTGPYKFVEWLKDERVVLEANPDYWGGKPAIDKVVFRPIAETRTRIAELTS
GGADIIVDVPPEDIAALDGGDTKVSTIASDSLYFLAFDTTRDTPLKDKRVRQAINYAVDVDAIQAALLNGMGTRIALTLP
TNAFAYDQAWKPYPYDPEKAKQLLAEAGYPEGFEIPFMSRKGRYMKDSEIIEATAGFLARVGIKANIQYLEPGVWAQVSE
RKGREGITFPGWAGRDPQLVWAPLLITGQYQSYFSNPELDELLKAGASIIDPEERKANYAKAAEIIKEEAPHLPMIQPPL
IYAIDAKLNWTPRSDGIIDLRKAHF
>Mature_504_residues
TFSFKRSLMAAAVGVSAVTLIAGSGLAQDNSTVVVAQAVDAYTMDPAKHSNFPTANILFQIYDGLIAEGENGELVPALAE
SWSNPDELTWRFKLREGVTFHNGEPFNAEAVKLSFERALDPEFKAPYFSRISHIKAVEVVDEYTVDFKTEEPFPTMLLSL
YEASFPALIVPPAYAKENDGASIAAHPVGTGPYKFVEWLKDERVVLEANPDYWGGKPAIDKVVFRPIAETRTRIAELTSG
GADIIVDVPPEDIAALDGGDTKVSTIASDSLYFLAFDTTRDTPLKDKRVRQAINYAVDVDAIQAALLNGMGTRIALTLPT
NAFAYDQAWKPYPYDPEKAKQLLAEAGYPEGFEIPFMSRKGRYMKDSEIIEATAGFLARVGIKANIQYLEPGVWAQVSER
KGREGITFPGWAGRDPQLVWAPLLITGQYQSYFSNPELDELLKAGASIIDPEERKANYAKAAEIIKEEAPHLPMIQPPLI
YAIDAKLNWTPRSDGIIDLRKAHF

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=492, Percent_Identity=30.4878048780488, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1789966, Length=437, Percent_Identity=30.2059496567506, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1787762, Length=508, Percent_Identity=26.7716535433071, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1787551, Length=482, Percent_Identity=27.1784232365145, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787495, Length=537, Percent_Identity=24.9534450651769, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789887, Length=507, Percent_Identity=26.232741617357, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081878, Length=507, Percent_Identity=24.8520710059172, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789397, Length=516, Percent_Identity=24.4186046511628, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786256, Length=244, Percent_Identity=27.8688524590164, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082063, Length=460, Percent_Identity=22.1739130434783, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55689; Mature: 55557

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSFKRSLMAAAVGVSAVTLIAGSGLAQDNSTVVVAQAVDAYTMDPAKHSNFPTANILF
CCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCEECCCHHCCCCCHHHHHH
QIYDGLIAEGENGELVPALAESWSNPDELTWRFKLREGVTFHNGEPFNAEAVKLSFERAL
HHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCCEEEEEHHHCC
DPEFKAPYFSRISHIKAVEVVDEYTVDFKTEEPFPTMLLSLYEASFPALIVPPAYAKEND
CCCCCCCHHHHHHHHHHEEEHHHHEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCC
GASIAAHPVGTGPYKFVEWLKDERVVLEANPDYWGGKPAIDKVVFRPIAETRTRIAELTS
CCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GGADIIVDVPPEDIAALDGGDTKVSTIASDSLYFLAFDTTRDTPLKDKRVRQAINYAVDV
CCCEEEEECCCHHHEECCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCH
DAIQAALLNGMGTRIALTLPTNAFAYDQAWKPYPYDPEKAKQLLAEAGYPEGFEIPFMSR
HHHHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
KGRYMKDSEIIEATAGFLARVGIKANIQYLEPGVWAQVSERKGREGITFPGWAGRDPQLV
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCEE
WAPLLITGQYQSYFSNPELDELLKAGASIIDPEERKANYAKAAEIIKEEAPHLPMIQPPL
EEEEEEECCHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IYAIDAKLNWTPRSDGIIDLRKAHF
EEEEECEEECCCCCCCEEEHHCCCC
>Mature Secondary Structure 
TFSFKRSLMAAAVGVSAVTLIAGSGLAQDNSTVVVAQAVDAYTMDPAKHSNFPTANILF
CCHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCEECCCHHCCCCCHHHHHH
QIYDGLIAEGENGELVPALAESWSNPDELTWRFKLREGVTFHNGEPFNAEAVKLSFERAL
HHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCCEEEEEHHHCC
DPEFKAPYFSRISHIKAVEVVDEYTVDFKTEEPFPTMLLSLYEASFPALIVPPAYAKEND
CCCCCCCHHHHHHHHHHEEEHHHHEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCC
GASIAAHPVGTGPYKFVEWLKDERVVLEANPDYWGGKPAIDKVVFRPIAETRTRIAELTS
CCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GGADIIVDVPPEDIAALDGGDTKVSTIASDSLYFLAFDTTRDTPLKDKRVRQAINYAVDV
CCCEEEEECCCHHHEECCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCH
DAIQAALLNGMGTRIALTLPTNAFAYDQAWKPYPYDPEKAKQLLAEAGYPEGFEIPFMSR
HHHHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
KGRYMKDSEIIEATAGFLARVGIKANIQYLEPGVWAQVSERKGREGITFPGWAGRDPQLV
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCEE
WAPLLITGQYQSYFSNPELDELLKAGASIIDPEERKANYAKAAEIIKEEAPHLPMIQPPL
EEEEEEECCHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IYAIDAKLNWTPRSDGIIDLRKAHF
EEEEECEEECCCCCCCEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA