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The map label for this gene is gcvA [H]

Identifier: 110634719

GI number: 110634719

Start: 2531264

End: 2532154

Strand: Reverse

Name: gcvA [H]

Synonym: Meso_2377

Alternate gene names: 110634719

Gene position: 2532154-2531264 (Counterclockwise)

Preceding gene: 110634722

Following gene: 110634717

Centisome position: 57.39

GC content: 64.53

Gene sequence:

>891_bases
ATGTCGGCCCACCGCAACCTGCCCCCCCTTCCCGCCCTGCGGATCTTCGAAGCTGCTTCCCGACGTCAGAGTTTTCTCCT
CGCGGCGAGGGAGCTGAATTTGACGCCCGGTGCCGTGAGCAAGCAAATCCGGCAGCTGGAGGAGCATCTGGGCTTGCCTC
TCTTCGAGCGACTGCATCGCGAGGTGCGCCTGACCGCGGCAGGAGCGATTTATGCTCGGGCCATCGGCCAGTTGTTCGAG
GACCTGCGGGCGGCGACGGCCGAGGTGATGGTCTCCCCGGCGGCGGACAAGCTGGTCGTGTGGTGCGCGCCGTGGTTCCT
CAAGGCTTGGCTGCTGCCGCGCCTCGAGCGGTTTCGCGCTTTGCGTCCCGGTCTGCAGATGGATCTTCGCACCGGCGAAA
GCAGCGAGGCCATCGATCCCAAGGCGGACATCGCCATCCGCTTGGGTCTTGGCACTTGGGCGCGTATGCGGTCGGAATTC
CTGATCCCGCAGGAGATCGCGGCCGTCTGCACGCCGGCTTATCTGCGGCGGGAGGGGTTGTCGCAGCCTTTTGCCTTCGG
GGATTTCGTTGTTCTGGATAGCGCGAACACCCCCCATCACCTTGAACTTTGGAAGCAGGCGGCGGGGATGGCCGGTGTCG
AACCGCGCGAGCGCATCGTCTTCCAGAGCAATGAAACCGCCTTTGCCGCTGCCCTCACAGATCACGGGCTAGCGCTCGTG
AACCCGTATTTCGTCGAGGCGGAGCTAGCGAATGGACGGCTCGTCTGTCCTTGGCCAGCAAGGACGCGGACCGATCGCAG
TTATTGGCTGACATGGCGGGATCGTTTCAACCTGCCGCCGGCCGCGAGCGCATTCCGCAGGTGGATTCTGGCCGAGTTCC
GACGCGAGTGA

Upstream 100 bases:

>100_bases
CCAAGTCTCCTCATTTTCATGCGAACAAACATCGATGGGCTCGGGTTGACAAACGACTCCTCCTCTGGTCAGGTGAGCAA
AATTCATCTGAACTCCGATC

Downstream 100 bases:

>100_bases
CTATACTGCGGACGCGATTAACCGGCGGGTGTACTCGTGCTGCGGATTGGCGAAGACATCGGCTGCGGGGCCGGCCTCGA
CGATCTCGCCCTTGTACATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSAHRNLPPLPALRIFEAASRRQSFLLAARELNLTPGAVSKQIRQLEEHLGLPLFERLHREVRLTAAGAIYARAIGQLFE
DLRAATAEVMVSPAADKLVVWCAPWFLKAWLLPRLERFRALRPGLQMDLRTGESSEAIDPKADIAIRLGLGTWARMRSEF
LIPQEIAAVCTPAYLRREGLSQPFAFGDFVVLDSANTPHHLELWKQAAGMAGVEPRERIVFQSNETAFAAALTDHGLALV
NPYFVEAELANGRLVCPWPARTRTDRSYWLTWRDRFNLPPAASAFRRWILAEFRRE

Sequences:

>Translated_296_residues
MSAHRNLPPLPALRIFEAASRRQSFLLAARELNLTPGAVSKQIRQLEEHLGLPLFERLHREVRLTAAGAIYARAIGQLFE
DLRAATAEVMVSPAADKLVVWCAPWFLKAWLLPRLERFRALRPGLQMDLRTGESSEAIDPKADIAIRLGLGTWARMRSEF
LIPQEIAAVCTPAYLRREGLSQPFAFGDFVVLDSANTPHHLELWKQAAGMAGVEPRERIVFQSNETAFAAALTDHGLALV
NPYFVEAELANGRLVCPWPARTRTDRSYWLTWRDRFNLPPAASAFRRWILAEFRRE
>Mature_295_residues
SAHRNLPPLPALRIFEAASRRQSFLLAARELNLTPGAVSKQIRQLEEHLGLPLFERLHREVRLTAAGAIYARAIGQLFED
LRAATAEVMVSPAADKLVVWCAPWFLKAWLLPRLERFRALRPGLQMDLRTGESSEAIDPKADIAIRLGLGTWARMRSEFL
IPQEIAAVCTPAYLRREGLSQPFAFGDFVVLDSANTPHHLELWKQAAGMAGVEPRERIVFQSNETAFAAALTDHGLALVN
PYFVEAELANGRLVCPWPARTRTDRSYWLTWRDRFNLPPAASAFRRWILAEFRRE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=33.448275862069, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=28.8079470198675, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=31.3993174061433, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=27.710843373494, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=26.7123287671233, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787949, Length=62, Percent_Identity=53.2258064516129, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790208, Length=141, Percent_Identity=32.6241134751773, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33488; Mature: 33357

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHRNLPPLPALRIFEAASRRQSFLLAARELNLTPGAVSKQIRQLEEHLGLPLFERLHR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
EVRLTAAGAIYARAIGQLFEDLRAATAEVMVSPAADKLVVWCAPWFLKAWLLPRLERFRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
LRPGLQMDLRTGESSEAIDPKADIAIRLGLGTWARMRSEFLIPQEIAAVCTPAYLRREGL
HCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCC
SQPFAFGDFVVLDSANTPHHLELWKQAAGMAGVEPRERIVFQSNETAFAAALTDHGLALV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHEEEECCCCEEEEHHHHCCEEEE
NPYFVEAELANGRLVCPWPARTRTDRSYWLTWRDRFNLPPAASAFRRWILAEFRRE
CCEEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAHRNLPPLPALRIFEAASRRQSFLLAARELNLTPGAVSKQIRQLEEHLGLPLFERLHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
EVRLTAAGAIYARAIGQLFEDLRAATAEVMVSPAADKLVVWCAPWFLKAWLLPRLERFRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
LRPGLQMDLRTGESSEAIDPKADIAIRLGLGTWARMRSEFLIPQEIAAVCTPAYLRREGL
HCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCC
SQPFAFGDFVVLDSANTPHHLELWKQAAGMAGVEPRERIVFQSNETAFAAALTDHGLALV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHEEEECCCCEEEEHHHHCCEEEE
NPYFVEAELANGRLVCPWPARTRTDRSYWLTWRDRFNLPPAASAFRRWILAEFRRE
CCEEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]