Click here to switch to the map view.

The map label for this gene is nfsA [C]

Identifier: 110634619

GI number: 110634619

Start: 2425637

End: 2426293

Strand: Reverse

Name: nfsA [C]

Synonym: Meso_2273

Alternate gene names: 110634619

Gene position: 2426293-2425637 (Counterclockwise)

Preceding gene: 110634632

Following gene: 110634610

Centisome position: 54.99

GC content: 61.04

Gene sequence:

>657_bases
ATGCCCGGACTGACGAGACGCATATTCATTGCATCGGCTGCATTGAGCGCTGGGATGACATCGAAATTGGCATCAGCTGG
CAAAGATGCCGCAGTGCCTGCAGGTGCAAAGCGTGGCGACATGCCGCTTCTGGAGGCGCTTTCCAAGCGACGCTCGACAA
GGCTTTTTTCCGACGAGACGATCGACGAAGCCACCCTTGCGACAATGCTCTGGTGCGCCTTCGGCATCAACCGCCCAGAC
AGCGGCTACCGGACCGCTCCGTCATGGCGAAGCTCGATGGAGACCGATATCTACGTGGCCGACACAGGCGGCGTGCGAAA
GTTCGACCCCTCTGCGGAGGCAATGGCACCCGCATTGAATGAAGACATCAGAAAGAAGGCGAGCCCGCAGCCCTTTGTGG
GGACTGCTCCCACGGTGCTGATCTATGTCGCTGACAGAAGCCGGATGGCGGAGGCCGCCGAGCCGGCGCAGATCCAGTCC
GCGCATGTGGACGCGGCAATCATTGCCCAGAACGTCTACCTATACTGTGCGTCCGCCGGGCTCGGAACGTGCCTGGTAGG
CGGCGCGGACAAGGAGGGGCTCAAGGCGCTCTTGAGGCTGCCCGACACGAAGCTGGTCACCTTCGTCCAGCCAATTGGCT
ATCCCAAGACGAATTGA

Upstream 100 bases:

>100_bases
ACACGCCGTTTCGAACGAACGGCCCTCCTTGATCGCCGTCAATGAGGCGCGTGCATCCGCCATCCAAATTCATCCGGGAA
CAAACAATGGAGATTTCGTT

Downstream 100 bases:

>100_bases
GCGGAGCCTGCTTCCGAGCGAGGATCGGCTTAAGATGCCGCTCAAAGAATTCCGTCACGCCGGCACGATTGAGATGATCC
GTGTCGGCGTAGAATTGCGG

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPGLTRRIFIASAALSAGMTSKLASAGKDAAVPAGAKRGDMPLLEALSKRRSTRLFSDETIDEATLATMLWCAFGINRPD
SGYRTAPSWRSSMETDIYVADTGGVRKFDPSAEAMAPALNEDIRKKASPQPFVGTAPTVLIYVADRSRMAEAAEPAQIQS
AHVDAAIIAQNVYLYCASAGLGTCLVGGADKEGLKALLRLPDTKLVTFVQPIGYPKTN

Sequences:

>Translated_218_residues
MPGLTRRIFIASAALSAGMTSKLASAGKDAAVPAGAKRGDMPLLEALSKRRSTRLFSDETIDEATLATMLWCAFGINRPD
SGYRTAPSWRSSMETDIYVADTGGVRKFDPSAEAMAPALNEDIRKKASPQPFVGTAPTVLIYVADRSRMAEAAEPAQIQS
AHVDAAIIAQNVYLYCASAGLGTCLVGGADKEGLKALLRLPDTKLVTFVQPIGYPKTN
>Mature_217_residues
PGLTRRIFIASAALSAGMTSKLASAGKDAAVPAGAKRGDMPLLEALSKRRSTRLFSDETIDEATLATMLWCAFGINRPDS
GYRTAPSWRSSMETDIYVADTGGVRKFDPSAEAMAPALNEDIRKKASPQPFVGTAPTVLIYVADRSRMAEAAEPAQIQSA
HVDAAIIAQNVYLYCASAGLGTCLVGGADKEGLKALLRLPDTKLVTFVQPIGYPKTN

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415
- InterPro:   IPR020051 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23125; Mature: 22994

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLTRRIFIASAALSAGMTSKLASAGKDAAVPAGAKRGDMPLLEALSKRRSTRLFSDET
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
IDEATLATMLWCAFGINRPDSGYRTAPSWRSSMETDIYVADTGGVRKFDPSAEAMAPALN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEECCCHHHHHHHHH
EDIRKKASPQPFVGTAPTVLIYVADRSRMAEAAEPAQIQSAHVDAAIIAQNVYLYCASAG
HHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHCCHHHHHHHEEEEEECCC
LGTCLVGGADKEGLKALLRLPDTKLVTFVQPIGYPKTN
CCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PGLTRRIFIASAALSAGMTSKLASAGKDAAVPAGAKRGDMPLLEALSKRRSTRLFSDET
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
IDEATLATMLWCAFGINRPDSGYRTAPSWRSSMETDIYVADTGGVRKFDPSAEAMAPALN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEECCCHHHHHHHHH
EDIRKKASPQPFVGTAPTVLIYVADRSRMAEAAEPAQIQSAHVDAAIIAQNVYLYCASAG
HHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHCCHHHHHHHEEEEEECCC
LGTCLVGGADKEGLKALLRLPDTKLVTFVQPIGYPKTN
CCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]