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The map label for this gene is leuA [H]

Identifier: 110634539

GI number: 110634539

Start: 2339521

End: 2341233

Strand: Reverse

Name: leuA [H]

Synonym: Meso_2190

Alternate gene names: 110634539

Gene position: 2341233-2339521 (Counterclockwise)

Preceding gene: 110634543

Following gene: 110634538

Centisome position: 53.06

GC content: 60.25

Gene sequence:

>1713_bases
ATGGACGCCAAGAACGAGATTTTGCATCGCAGCACCCGCAAGGGAATGCCGACCGCCCCGCGCAAATATCAGCCCTATCC
CTTTGTCGACCTTTCCGACCGCACCTGGCCCTCCAAGCGGATAGAAAAGGCGCCGGTCTGGTGCTCGGTGGATCTGCGCG
ACGGCAATCAGGCCCTAATTGACCCCATGGGGCATGATCGCAAGGCGCGGATGTTCGCTCTCCTCCTCGATATGGGCTTT
CGGGAGATAGAAATCGGCTTTCCCTCGGCCTCTCAGACCGATTTCGACTTCACGCGCTGGGCAATCGAAGAGGGCAATGT
GCCCGACGACGTCTCCCTCCAGGTGTTGGTGCAGTGCCGGCCAGAGCTGATCGCGCGCACTTTCGAGGCGCTCGAGGGGG
CCACGCATCCGATCGTCCATTTCTACAACTCCACCAGCGAGTTGCAGCGTCGCGTTGTGTTTGAAAAGGATGTCGCCGGC
ATCAAGACGATCGCCACCGATGCCGCCAAGATGATCACGGATATGGCTGCGAAAGCGGGCGGGGGCTACCGCTTCCAGTA
TTCGCCCGAGAGCTTTACCGGCACCGAGCTGGATGTGGCGCTGGAGATCTGCAACGCGGTGATCGGGATCGTCCAGCCCA
CGCCGGAAGACAAGCTCATCATCAACCTGCCATCCACGGTGGAGATGTCGACGCCCAACATCTATGCCGACCAGATCGAG
TGGATGTGCCGGCAGCTCGACCGGCGCGACAGCCTGATCGTCTCGTTGCACCCGCACAACGACAGGGGCACGGCCATCGC
GGCCACCGAACTCGGCTTGATGGCAGGCGCGGACCGTGTGGAAGGAACGCTGTTCGGCAACGGCGAGCGGACGGGCAATG
TCGACATCGTCACGCTGGCGCTCAACATGTACACGCAGGGCGTCGATCCCGTGCTCGACTGTTCGGAAATCAACCGCATC
AAGGATGTCTACGAATATTCCAACCAGCTCCGCATCCCGGAACGGCACCCCTATGTGGGCGAATTGGTCTACACGGCCTT
CTCCGGCTCCCATCAGGACGCCATCAACAAGGGCATGAAGGCCTACCGCAAGGCCAATAAGGAGCTGTGGGAGGTGCCGT
ATCTTCCCATCGATCCCGCCGATGTGGGTCGCACCTATGAGGCGATCATCCGCATCAACTCGCAGTCAGGCAAGGGCGGC
ATCGCCTATGTGCTGCAAGCCGATTACGGGCTCAACCTGCCGCGCAACCTGCAGGTGGAGTTTCGCGAGGACATCCAGGC
CATCACCGACGCGGAAGGGAAGGAACTGCCTTCCAAGCGTATCCATGACCGTTTTCTGGAGGTCTATGTCGATCAGCCCG
GAGCACGGATCAAATTGATCGATCACCAGACCTTTCCCGATCCCGAGATGAAGGGCCGGCGCATTCTCGAAGCCACGATC
CTCGACAACGGCAAGGAGACGGTGATCAAGGGAACCGGCACCGGGCCGATCGACGGGATCGTCAACGCGCTGTCGCGGCA
TATCGGTATGGAGATATCGGTTCTCGATTATTCGGAGCACTCGCTCCAGCGCGGCACCGACGCCGCCGCCGTCTCTTATG
TGGAGATGGAATTTCCGGGCGGAAAGCTCTTTGGTGTCGGCATCAATACCAATATTACGACTGCCTCGCTGGAAGCGGTC
GTCTCCGCGGTCAATCGCATTCTAAGGGTGTAG

Upstream 100 bases:

>100_bases
GGTGATCGCTGGTCTGCTCCTTCCCGTCAGAAACTGGATTTGAAGCCTTGACAGGCGGGCCGTCGCTTCCGAGCAACGTG
CTACCGCCCGGAGTGAGCAA

Downstream 100 bases:

>100_bases
AAAAATAGGGCAGTAGGGGCCATGCAGCCGGTTCGTTTTCTCCTATTGCCTTATTTCCCCACTCTCTTGCTACCTTAATC
GTGTGATGATTTTCCTATTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MDAKNEILHRSTRKGMPTAPRKYQPYPFVDLSDRTWPSKRIEKAPVWCSVDLRDGNQALIDPMGHDRKARMFALLLDMGF
REIEIGFPSASQTDFDFTRWAIEEGNVPDDVSLQVLVQCRPELIARTFEALEGATHPIVHFYNSTSELQRRVVFEKDVAG
IKTIATDAAKMITDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIGIVQPTPEDKLIINLPSTVEMSTPNIYADQIE
WMCRQLDRRDSLIVSLHPHNDRGTAIAATELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPVLDCSEINRI
KDVYEYSNQLRIPERHPYVGELVYTAFSGSHQDAINKGMKAYRKANKELWEVPYLPIDPADVGRTYEAIIRINSQSGKGG
IAYVLQADYGLNLPRNLQVEFREDIQAITDAEGKELPSKRIHDRFLEVYVDQPGARIKLIDHQTFPDPEMKGRRILEATI
LDNGKETVIKGTGTGPIDGIVNALSRHIGMEISVLDYSEHSLQRGTDAAAVSYVEMEFPGGKLFGVGINTNITTASLEAV
VSAVNRILRV

Sequences:

>Translated_570_residues
MDAKNEILHRSTRKGMPTAPRKYQPYPFVDLSDRTWPSKRIEKAPVWCSVDLRDGNQALIDPMGHDRKARMFALLLDMGF
REIEIGFPSASQTDFDFTRWAIEEGNVPDDVSLQVLVQCRPELIARTFEALEGATHPIVHFYNSTSELQRRVVFEKDVAG
IKTIATDAAKMITDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIGIVQPTPEDKLIINLPSTVEMSTPNIYADQIE
WMCRQLDRRDSLIVSLHPHNDRGTAIAATELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPVLDCSEINRI
KDVYEYSNQLRIPERHPYVGELVYTAFSGSHQDAINKGMKAYRKANKELWEVPYLPIDPADVGRTYEAIIRINSQSGKGG
IAYVLQADYGLNLPRNLQVEFREDIQAITDAEGKELPSKRIHDRFLEVYVDQPGARIKLIDHQTFPDPEMKGRRILEATI
LDNGKETVIKGTGTGPIDGIVNALSRHIGMEISVLDYSEHSLQRGTDAAAVSYVEMEFPGGKLFGVGINTNITTASLEAV
VSAVNRILRV
>Mature_570_residues
MDAKNEILHRSTRKGMPTAPRKYQPYPFVDLSDRTWPSKRIEKAPVWCSVDLRDGNQALIDPMGHDRKARMFALLLDMGF
REIEIGFPSASQTDFDFTRWAIEEGNVPDDVSLQVLVQCRPELIARTFEALEGATHPIVHFYNSTSELQRRVVFEKDVAG
IKTIATDAAKMITDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIGIVQPTPEDKLIINLPSTVEMSTPNIYADQIE
WMCRQLDRRDSLIVSLHPHNDRGTAIAATELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPVLDCSEINRI
KDVYEYSNQLRIPERHPYVGELVYTAFSGSHQDAINKGMKAYRKANKELWEVPYLPIDPADVGRTYEAIIRINSQSGKGG
IAYVLQADYGLNLPRNLQVEFREDIQAITDAEGKELPSKRIHDRFLEVYVDQPGARIKLIDHQTFPDPEMKGRRILEATI
LDNGKETVIKGTGTGPIDGIVNALSRHIGMEISVLDYSEHSLQRGTDAAAVSYVEMEFPGGKLFGVGINTNITTASLEAV
VSAVNRILRV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=532, Percent_Identity=24.0601503759398, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=564, Percent_Identity=45.9219858156028, Blast_Score=511, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6324682, Length=575, Percent_Identity=45.7391304347826, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63431; Mature: 63431

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKNEILHRSTRKGMPTAPRKYQPYPFVDLSDRTWPSKRIEKAPVWCSVDLRDGNQALI
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHCCCCEEEEEEECCCCEEEE
DPMGHDRKARMFALLLDMGFREIEIGFPSASQTDFDFTRWAIEEGNVPDDVSLQVLVQCR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHECCC
PELIARTFEALEGATHPIVHFYNSTSELQRRVVFEKDVAGIKTIATDAAKMITDMAAKAG
HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GGYRFQYSPESFTGTELDVALEICNAVIGIVQPTPEDKLIINLPSTVEMSTPNIYADQIE
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCEEHHHHH
WMCRQLDRRDSLIVSLHPHNDRGTAIAATELGLMAGADRVEGTLFGNGERTGNVDIVTLA
HHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEEEE
LNMYTQGVDPVLDCSEINRIKDVYEYSNQLRIPERHPYVGELVYTAFSGSHQDAINKGMK
EEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHH
AYRKANKELWEVPYLPIDPADVGRTYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQVE
HHHHHCCHHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCH
FREDIQAITDAEGKELPSKRIHDRFLEVYVDQPGARIKLIDHQTFPDPEMKGRRILEATI
HHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEE
LDNGKETVIKGTGTGPIDGIVNALSRHIGMEISVLDYSEHSLQRGTDAAAVSYVEMEFPG
ECCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCEEEEEEEECCC
GKLFGVGINTNITTASLEAVVSAVNRILRV
CEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAKNEILHRSTRKGMPTAPRKYQPYPFVDLSDRTWPSKRIEKAPVWCSVDLRDGNQALI
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHCCCCEEEEEEECCCCEEEE
DPMGHDRKARMFALLLDMGFREIEIGFPSASQTDFDFTRWAIEEGNVPDDVSLQVLVQCR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHECCC
PELIARTFEALEGATHPIVHFYNSTSELQRRVVFEKDVAGIKTIATDAAKMITDMAAKAG
HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GGYRFQYSPESFTGTELDVALEICNAVIGIVQPTPEDKLIINLPSTVEMSTPNIYADQIE
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCEEHHHHH
WMCRQLDRRDSLIVSLHPHNDRGTAIAATELGLMAGADRVEGTLFGNGERTGNVDIVTLA
HHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEEEE
LNMYTQGVDPVLDCSEINRIKDVYEYSNQLRIPERHPYVGELVYTAFSGSHQDAINKGMK
EEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHH
AYRKANKELWEVPYLPIDPADVGRTYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQVE
HHHHHCCHHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCH
FREDIQAITDAEGKELPSKRIHDRFLEVYVDQPGARIKLIDHQTFPDPEMKGRRILEATI
HHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEE
LDNGKETVIKGTGTGPIDGIVNALSRHIGMEISVLDYSEHSLQRGTDAAAVSYVEMEFPG
ECCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCEEEEEEEECCC
GKLFGVGINTNITTASLEAVVSAVNRILRV
CEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]