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The map label for this gene is trmL [H]

Identifier: 110634520

GI number: 110634520

Start: 2319213

End: 2319674

Strand: Reverse

Name: trmL [H]

Synonym: Meso_2171

Alternate gene names: 110634520

Gene position: 2319674-2319213 (Counterclockwise)

Preceding gene: 110634525

Following gene: 110634518

Centisome position: 52.57

GC content: 62.12

Gene sequence:

>462_bases
ATGCCGCAACTGGGCTTCCGTATCGCACTTTTCCAGCCCGACATTCCGGGCAATACCGGCGCGATACTGCGCCTTTCGGC
CTGTCTTGGCCTGGCCGTCGACCTGATCGAGCCGGCCGGCTTCGACCTTTCCGACCGCGCACTGAAGCGGGCGGGCATGG
ACTATCTGGAGCTTGCCGCGCTTCGGCGCCATGCCTCGTGGGAGGATTTCAATAGCTGGCGGCACCAGGAAGCGCGCCGG
ATCATCCTGTTCACGACGGCAGGAACGGTTCCTTACCAATGTTTTTCTTTCCGGGACGGGGACATCCTCATGTTCGGCCG
GGAATCATCCGGCGTGCCGGATCATGTCCACAACGCAGTCAATTATCGCCTGGTCATTCCAATGAAGGAAGGAACGCGCA
GCCTGAATGTGGCGATGGCCGCCGCGATGGCCTGCGGTGAAGCGGTGCGGCAGGTGCGCTGA

Upstream 100 bases:

>100_bases
CGGCGTTATATCCATTCAGGCGCGACATGGCCAGACGCTTTGGCAAGGCAGTGGTGCCTCAACCGCCGGCTGTGGCTTTC
GCGTGACAGTAAGGGACAAC

Downstream 100 bases:

>100_bases
GGCTTCCCGCAGGAAGGTATCAATCCGCGCTCGGCAGGCGCCGCATGGTGAATTCGATCACATCGCCCGGACGCTCGGCC
CAGCTTTCGATTTCCGTCCA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPQLGFRIALFQPDIPGNTGAILRLSACLGLAVDLIEPAGFDLSDRALKRAGMDYLELAALRRHASWEDFNSWRHQEARR
IILFTTAGTVPYQCFSFRDGDILMFGRESSGVPDHVHNAVNYRLVIPMKEGTRSLNVAMAAAMACGEAVRQVR

Sequences:

>Translated_153_residues
MPQLGFRIALFQPDIPGNTGAILRLSACLGLAVDLIEPAGFDLSDRALKRAGMDYLELAALRRHASWEDFNSWRHQEARR
IILFTTAGTVPYQCFSFRDGDILMFGRESSGVPDHVHNAVNYRLVIPMKEGTRSLNVAMAAAMACGEAVRQVR
>Mature_152_residues
PQLGFRIALFQPDIPGNTGAILRLSACLGLAVDLIEPAGFDLSDRALKRAGMDYLELAALRRHASWEDFNSWRHQEARRI
ILFTTAGTVPYQCFSFRDGDILMFGRESSGVPDHVHNAVNYRLVIPMKEGTRSLNVAMAAAMACGEAVRQVR

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=38.9261744966443, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16978; Mature: 16847

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLGFRIALFQPDIPGNTGAILRLSACLGLAVDLIEPAGFDLSDRALKRAGMDYLELAA
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
LRRHASWEDFNSWRHQEARRIILFTTAGTVPYQCFSFRDGDILMFGRESSGVPDHVHNAV
HHHCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHCCC
NYRLVIPMKEGTRSLNVAMAAAMACGEAVRQVR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQLGFRIALFQPDIPGNTGAILRLSACLGLAVDLIEPAGFDLSDRALKRAGMDYLELAA
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
LRRHASWEDFNSWRHQEARRIILFTTAGTVPYQCFSFRDGDILMFGRESSGVPDHVHNAV
HHHCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHCCC
NYRLVIPMKEGTRSLNVAMAAAMACGEAVRQVR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA