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The map label for this gene is amiD [H]

Identifier: 110634368

GI number: 110634368

Start: 2166890

End: 2167645

Strand: Reverse

Name: amiD [H]

Synonym: Meso_2018

Alternate gene names: 110634368

Gene position: 2167645-2166890 (Counterclockwise)

Preceding gene: 110634369

Following gene: 110634367

Centisome position: 49.13

GC content: 60.98

Gene sequence:

>756_bases
ATGACCGGTTTTCCGCCCGATTTTCCCGAGGCGGCGGTGCGGCCCTCTCCAAATTTCGGCGAGAGGCGAAAGGGGCGCAA
GCCCGATGCTATCATCTTGCACTACACCGGAATGACGAGCGGCCAGGCGGCGGAGGATTGGCTTTGCAATCCGACCAGCG
AAGTTTCTTCGCATTATCTGGCGCACGAGGATGGGCGCGTGGTCCAGATGGTTCCCGAAGCAGCGCGCGCCTGGCATGCG
GGCAGGGGCAGCTGGAAGGGCGAGGACGACGTCAATTCGTTCTCCATCGGAGTGGAGATCGTCAATCCGGGTCACCAGTT
CGGCTATCGCGACTTTCCCTCTGTCCAGATCGACGCCGTGGCCGCTCTTTGCCTGGATATCTGCCGCCGGAACGCAATTC
TGCCACAAAGGGTGCTCGCCCATTCCGACACCGCACCCGGCCGCAAGGTCGATCCGGGCGAAAAATTTCCATGGAAGCGA
CTCGCCGAGATGGGCGTTGGCCATTACGTCGATCCGGAGCGGGTCACCGGCGGCGAGGTGCTTGCGCCCGGTGATCGGGG
CTCTGCGGTCGCGGGGCTACAACGCCGCTTGGCTGAATATGGCTACGGAATTGAAAAAACGGGCGTTTTCGATGCCGAAA
CCGAAACGGTCGTTGCTGCGTTCCAGCGGCATTTCCGCCCTTCAGCCGTCAATGGCATTGCCGATTCTTCGACATGCTCG
ACACTCGAAAAGCTGATTTTGGCCCTGCCGAAATAG

Upstream 100 bases:

>100_bases
TTGCACGCGGAGTGCCCATGGAGTTCCTGGCCATTGCTAATGCCCGGCTTGCGGCGATCAATGCCGCTTTCGAGCAGATC
GAGAAGAGCTGGCTACCGGT

Downstream 100 bases:

>100_bases
ATGAATTAAGCGGCCTTTTCGTCGAGATATTACAACCTGTATTGTTTTTTTATCAAGTCAGCCCTTTTTGGTTCATCTTC
CACCTTAAAACCGAGCCTCA

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTGFPPDFPEAAVRPSPNFGERRKGRKPDAIILHYTGMTSGQAAEDWLCNPTSEVSSHYLAHEDGRVVQMVPEAARAWHA
GRGSWKGEDDVNSFSIGVEIVNPGHQFGYRDFPSVQIDAVAALCLDICRRNAILPQRVLAHSDTAPGRKVDPGEKFPWKR
LAEMGVGHYVDPERVTGGEVLAPGDRGSAVAGLQRRLAEYGYGIEKTGVFDAETETVVAAFQRHFRPSAVNGIADSSTCS
TLEKLILALPK

Sequences:

>Translated_251_residues
MTGFPPDFPEAAVRPSPNFGERRKGRKPDAIILHYTGMTSGQAAEDWLCNPTSEVSSHYLAHEDGRVVQMVPEAARAWHA
GRGSWKGEDDVNSFSIGVEIVNPGHQFGYRDFPSVQIDAVAALCLDICRRNAILPQRVLAHSDTAPGRKVDPGEKFPWKR
LAEMGVGHYVDPERVTGGEVLAPGDRGSAVAGLQRRLAEYGYGIEKTGVFDAETETVVAAFQRHFRPSAVNGIADSSTCS
TLEKLILALPK
>Mature_250_residues
TGFPPDFPEAAVRPSPNFGERRKGRKPDAIILHYTGMTSGQAAEDWLCNPTSEVSSHYLAHEDGRVVQMVPEAARAWHAG
RGSWKGEDDVNSFSIGVEIVNPGHQFGYRDFPSVQIDAVAALCLDICRRNAILPQRVLAHSDTAPGRKVDPGEKFPWKRL
AEMGVGHYVDPERVTGGEVLAPGDRGSAVAGLQRRLAEYGYGIEKTGVFDAETETVVAAFQRHFRPSAVNGIADSSTCST
LEKLILALPK

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=236, Percent_Identity=38.5593220338983, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786300, Length=164, Percent_Identity=31.0975609756098, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27295; Mature: 27164

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFPPDFPEAAVRPSPNFGERRKGRKPDAIILHYTGMTSGQAAEDWLCNPTSEVSSHYL
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCHHCCCCCHHHHHHHHH
AHEDGRVVQMVPEAARAWHAGRGSWKGEDDVNSFSIGVEIVNPGHQFGYRDFPSVQIDAV
HCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHCCCCCCCCHHHHHH
AALCLDICRRNAILPQRVLAHSDTAPGRKVDPGEKFPWKRLAEMGVGHYVDPERVTGGEV
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
LAPGDRGSAVAGLQRRLAEYGYGIEKTGVFDAETETVVAAFQRHFRPSAVNGIADSSTCS
ECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHH
TLEKLILALPK
HHHHHHHHCCC
>Mature Secondary Structure 
TGFPPDFPEAAVRPSPNFGERRKGRKPDAIILHYTGMTSGQAAEDWLCNPTSEVSSHYL
CCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCHHCCCCCHHHHHHHHH
AHEDGRVVQMVPEAARAWHAGRGSWKGEDDVNSFSIGVEIVNPGHQFGYRDFPSVQIDAV
HCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHCCCCCCCCHHHHHH
AALCLDICRRNAILPQRVLAHSDTAPGRKVDPGEKFPWKRLAEMGVGHYVDPERVTGGEV
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
LAPGDRGSAVAGLQRRLAEYGYGIEKTGVFDAETETVVAAFQRHFRPSAVNGIADSSTCS
ECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHH
TLEKLILALPK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]