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The map label for this gene is murD

Identifier: 110634359

GI number: 110634359

Start: 2156082

End: 2157485

Strand: Reverse

Name: murD

Synonym: Meso_2009

Alternate gene names: 110634359

Gene position: 2157485-2156082 (Counterclockwise)

Preceding gene: 110634360

Following gene: 110634358

Centisome position: 48.9

GC content: 64.53

Gene sequence:

>1404_bases
ATGATCCCCGCCACCACCTTCCACGGCAGACAAGTCGCGCTGTTCGGCCTCGGCGGTTCGGGCATCGCGACCGCCCAGGC
GATTATGGCCGGCGGTGGCGAGGTGCTTGCCTGGGACGATAATCCCGAAAGCGTGGCCAAGGCCAAGGCCGAAGGCATTA
AGACCGGTGATCTGCGCGATGCGGACTGGCGCTCAATCCACACGCTCTTGCTCGCTCCCGGCGTGCCGCTCACCCATCCC
CACCCGCATTGGAGCGTGGAGCTGGCGCGATCGGCGGGCGTCGAGATCATCGGCGATATCGAGCTTTTCGTGCGGGAGCG
GCGGATGCATGCCGCGCACGCCCCGCTGGTGGCGATCACCGGCACGAACGGGAAATCGACGACGACGGCGCTCACGGCCC
ATGTGCTGAAGGCCGCCGGACGCGATACCCAGATGGGGGGCAATATCGGCCGCGCGGTGATGACGCTCGATCCGCCCGTT
GCGGATCGTCATTACGTGCTCGAATGCTCCTCCTATCAGATCGACCTTTCGCCGACGCTCAATCCCACTGCCGGCGTGCT
CCTCAACATCACGCCCGACCATCTCGACCGGCATGGCACGATGGAGAATTACGCTGCCATCAAGGAGCGGCTGGTGGCGG
GCAGTGACACGGCGATTATCGGGGTGGACGACGAATATTGCCGCGCCGTCGCCGACCGGCTGGAGAAGGCTGGCCGGAAA
GTTTCGCGGATTTCCCTGACCGGCACGCTGGAGGACGGTTTCTTCGCTGATGGAACCCAGTTGTTTCGCGCCGGGCAGGG
CAAGGCCGAGCCGCTTTTCTCGCTCGAAGGCATCGGGTCCCTGCGCGGCCGGCACAACGCCCAAAATGCGTTGGCCGCGG
CGGCCGCATGCCTTGCCTGCGGTCTCTCGCCTGCGGAAATCCAAACGGGGTTCAGGAGCTTCCCGGGGCTTGCTCACCGC
ATGGAACAGGTGGGCAAGTTGGGACACGTGCTCTTCGTCAACGATTCGAAGGCCACCAATGCCGATGCCTCGGCTCCGGC
CCTCTCGTCCTTCCCGCGCATCTATTGGATCGCCGGTGGATTGCCCAAGGCCGGCGGTATCGCGAGCCTGAGACAGTATT
TCCCGCGCATCGCCAAGGCCTATCTCATTGGCGAGGCGGCACCCGCCTTTGCCGCCACGATCGGAGAGGCGACAAATTAC
GAGATCTCCGGTACCATCGCAGCCGCAGTTCAGCACGCCGCGCGGGATGCGGCCGATGATCCCGCCGGCGATGTGGTGGT
CCTGTTGTCACCGGCCTGTGCGAGTTTCGATCAGTTCAGAAATTTCGAGGTCAGGGGTGAAGCGTTTCGCGATGCGGTGC
GCGCCCTGCCGGGCATTGAGCTTATTGGGGAGAAGAGGGCATGA

Upstream 100 bases:

>100_bases
GGATCATTGCCGTCGTCCTGGCGCTGATCGGGCTTTCAACGCTGAAGCTGCGGTAGGGGGTGGGAATGGTCCGGCTGTGT
ATCCAGGTGGGGCTTTTCCA

Downstream 100 bases:

>100_bases
TCAGCCGTACCGACCGCTCCATGGTGGCGAACTGGTGGTGGACTGTCGACCGATGGTTCCTTGCCGCATTCCTCTTTCTC
ATGGGACTCGGCGTCGTGCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIPATTFHGRQVALFGLGGSGIATAQAIMAGGGEVLAWDDNPESVAKAKAEGIKTGDLRDADWRSIHTLLLAPGVPLTHP
HPHWSVELARSAGVEIIGDIELFVRERRMHAAHAPLVAITGTNGKSTTTALTAHVLKAAGRDTQMGGNIGRAVMTLDPPV
ADRHYVLECSSYQIDLSPTLNPTAGVLLNITPDHLDRHGTMENYAAIKERLVAGSDTAIIGVDDEYCRAVADRLEKAGRK
VSRISLTGTLEDGFFADGTQLFRAGQGKAEPLFSLEGIGSLRGRHNAQNALAAAAACLACGLSPAEIQTGFRSFPGLAHR
MEQVGKLGHVLFVNDSKATNADASAPALSSFPRIYWIAGGLPKAGGIASLRQYFPRIAKAYLIGEAAPAFAATIGEATNY
EISGTIAAAVQHAARDAADDPAGDVVVLLSPACASFDQFRNFEVRGEAFRDAVRALPGIELIGEKRA

Sequences:

>Translated_467_residues
MIPATTFHGRQVALFGLGGSGIATAQAIMAGGGEVLAWDDNPESVAKAKAEGIKTGDLRDADWRSIHTLLLAPGVPLTHP
HPHWSVELARSAGVEIIGDIELFVRERRMHAAHAPLVAITGTNGKSTTTALTAHVLKAAGRDTQMGGNIGRAVMTLDPPV
ADRHYVLECSSYQIDLSPTLNPTAGVLLNITPDHLDRHGTMENYAAIKERLVAGSDTAIIGVDDEYCRAVADRLEKAGRK
VSRISLTGTLEDGFFADGTQLFRAGQGKAEPLFSLEGIGSLRGRHNAQNALAAAAACLACGLSPAEIQTGFRSFPGLAHR
MEQVGKLGHVLFVNDSKATNADASAPALSSFPRIYWIAGGLPKAGGIASLRQYFPRIAKAYLIGEAAPAFAATIGEATNY
EISGTIAAAVQHAARDAADDPAGDVVVLLSPACASFDQFRNFEVRGEAFRDAVRALPGIELIGEKRA
>Mature_467_residues
MIPATTFHGRQVALFGLGGSGIATAQAIMAGGGEVLAWDDNPESVAKAKAEGIKTGDLRDADWRSIHTLLLAPGVPLTHP
HPHWSVELARSAGVEIIGDIELFVRERRMHAAHAPLVAITGTNGKSTTTALTAHVLKAAGRDTQMGGNIGRAVMTLDPPV
ADRHYVLECSSYQIDLSPTLNPTAGVLLNITPDHLDRHGTMENYAAIKERLVAGSDTAIIGVDDEYCRAVADRLEKAGRK
VSRISLTGTLEDGFFADGTQLFRAGQGKAEPLFSLEGIGSLRGRHNAQNALAAAAACLACGLSPAEIQTGFRSFPGLAHR
MEQVGKLGHVLFVNDSKATNADASAPALSSFPRIYWIAGGLPKAGGIASLRQYFPRIAKAYLIGEAAPAFAATIGEATNY
EISGTIAAAVQHAARDAADDPAGDVVVLLSPACASFDQFRNFEVRGEAFRDAVRALPGIELIGEKRA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=34.8484848484849, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_MESSB (Q11GS3)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674567.1
- ProteinModelPortal:   Q11GS3
- STRING:   Q11GS3
- GeneID:   4182802
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2009
- NMPDR:   fig|266779.1.peg.604
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q11GS3
- ProtClustDB:   PRK01390
- BioCyc:   MSP266779:MESO_2009-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49161; Mature: 49161

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPATTFHGRQVALFGLGGSGIATAQAIMAGGGEVLAWDDNPESVAKAKAEGIKTGDLRD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCC
ADWRSIHTLLLAPGVPLTHPHPHWSVELARSAGVEIIGDIELFVRERRMHAAHAPLVAIT
CCHHHHHHHEEECCCCCCCCCCCCEEEEHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEE
GTNGKSTTTALTAHVLKAAGRDTQMGGNIGRAVMTLDPPVADRHYVLECSSYQIDLSPTL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGVLLNITPDHLDRHGTMENYAAIKERLVAGSDTAIIGVDDEYCRAVADRLEKAGRK
CCCCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCE
VSRISLTGTLEDGFFADGTQLFRAGQGKAEPLFSLEGIGSLRGRHNAQNALAAAAACLAC
EEEEEEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCCHHHCCCCCHHHHHHHHHHHHHH
GLSPAEIQTGFRSFPGLAHRMEQVGKLGHVLFVNDSKATNADASAPALSSFPRIYWIAGG
CCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECC
LPKAGGIASLRQYFPRIAKAYLIGEAAPAFAATIGEATNYEISGTIAAAVQHAARDAADD
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCC
PAGDVVVLLSPACASFDQFRNFEVRGEAFRDAVRALPGIELIGEKRA
CCCCEEEEECCCHHCHHHHCCCEECCHHHHHHHHHCCCCEECCCCCC
>Mature Secondary Structure
MIPATTFHGRQVALFGLGGSGIATAQAIMAGGGEVLAWDDNPESVAKAKAEGIKTGDLRD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCC
ADWRSIHTLLLAPGVPLTHPHPHWSVELARSAGVEIIGDIELFVRERRMHAAHAPLVAIT
CCHHHHHHHEEECCCCCCCCCCCCEEEEHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEE
GTNGKSTTTALTAHVLKAAGRDTQMGGNIGRAVMTLDPPVADRHYVLECSSYQIDLSPTL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGVLLNITPDHLDRHGTMENYAAIKERLVAGSDTAIIGVDDEYCRAVADRLEKAGRK
CCCCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCE
VSRISLTGTLEDGFFADGTQLFRAGQGKAEPLFSLEGIGSLRGRHNAQNALAAAAACLAC
EEEEEEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCCHHHCCCCCHHHHHHHHHHHHHH
GLSPAEIQTGFRSFPGLAHRMEQVGKLGHVLFVNDSKATNADASAPALSSFPRIYWIAGG
CCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECC
LPKAGGIASLRQYFPRIAKAYLIGEAAPAFAATIGEATNYEISGTIAAAVQHAARDAADD
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCC
PAGDVVVLLSPACASFDQFRNFEVRGEAFRDAVRALPGIELIGEKRA
CCCCEEEEECCCHHCHHHHCCCEECCHHHHHHHHHCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA