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The map label for this gene is tuf2 [H]

Identifier: 110634176

GI number: 110634176

Start: 1960566

End: 1961741

Strand: Reverse

Name: tuf2 [H]

Synonym: Meso_1826

Alternate gene names: 110634176

Gene position: 1961741-1960566 (Counterclockwise)

Preceding gene: 110634178

Following gene: 110634175

Centisome position: 44.46

GC content: 63.69

Gene sequence:

>1176_bases
ATGGCCAAAGGTAAATTTGAGCGCACGAAGCCGCATGTGAACATCGGGACGATTGGTCACGTCGACCATGGGAAGACGTC
TCTGACGGCCGCGATCACGAAGTATTTCGGTGAGTTCAAGGCTTACGATCAGATTGACGCGGCGCCTGAGGAAAAGGCGC
GCGGCATCACGATCTCGACGGCGCATGTGGAATACGAGACGGAGAACCGGCACTACGCGCATGTGGACTGCCCCGGCCAC
GCGGACTATGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCGATCCTTGTTGTTTCGGCCGCCGACGGCCC
GATGCCGCAGACGCGCGAGCACATTCTTCTTGCGCGCCAGGTGGGCGTTCCGGCGATCGTGGTGTTCCTGAACAAGGTTG
ACCAGGTGGACGACCCGGAGCTTCTGGAGCTGGTGGAGCTGGAGATCCGCGAGCTTCTGTCGAAATACGAGTTCCCGGGC
GACGACATTCCGATCGTCAAGGGCTCGGCGCTTGCGGCGCTTGAGGATTCGAACAAGGAGATCGGCGAGGACGCGGTGCG
CCAGCTGATGGCCGAGGTCGACAAGTACATCCCGACGCCGGAGCGTCCGATCGACCAGCCGTTCCTGATGCCTATCGAGG
ACGTGTTCTCGATTTCGGGCCGCGGCACGGTGGTGACGGGGCGCGTTGAGCGCGGCGTTGTGAAGGTGGGCGAGGAAGTC
GAGATCGTCGGCATCCGTCCGACCTCGAAGACGACGGTGACGGGCGTGGAGATGTTCCGCAAGCTGCTCGACCAGGGGCA
GGCGGGCGACAATATCGGCGCGCTTTTGCGCGGCATCGACCGTGAGGGCGTCGAGCGCGGGCAGGTTCTTGCCAAGCCGG
GCTCGGTGACCCCGCACACGAAGTTCAAGGCCGAGGCCTATATCCTGACCAAGGAGGAGGGCGGGCGCCACACGCCGTTC
TTCACCAACTACCGGCCGCAATTCTATTTCCGCACGACGGACGTGACGGGCGTCGTGACGCTGCCGGAAGGCACGGAGAT
GGTGATGCCGGGCGACAATGTGACGATGGACGTGACGCTGATCGTGCCGATCGCCATGGAGGAGCGCCTGCGCTTCGCCA
TCCGCGAAGGCGGCCGCACCGTCGGGGCCGGCATCGTCGCCAGCATCACAGAGTAA

Upstream 100 bases:

>100_bases
GCCGCGAGCCTATAGGCGATAGGGACTTGTTTTTTGCGGTCTTTGTCGTTAATCGCCCCGCAACCAAAGTCCACAACTAC
GCCGCCCAAGGGAATCTGAG

Downstream 100 bases:

>100_bases
AGAGGCAGCGGATTTCCCGTTTGGAAGGGCAGCTTCGGCTGCCCTTTCTTTTTAGTGTTGATAGCGGCGGCCACGCTTTC
CATATGAGGCGCGTCACGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDPELLELVELEIRELLSKYEFPG
DDIPIVKGSALAALEDSNKEIGEDAVRQLMAEVDKYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEV
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGVERGQVLAKPGSVTPHTKFKAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVTLIVPIAMEERLRFAIREGGRTVGAGIVASITE

Sequences:

>Translated_391_residues
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDPELLELVELEIRELLSKYEFPG
DDIPIVKGSALAALEDSNKEIGEDAVRQLMAEVDKYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEV
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGVERGQVLAKPGSVTPHTKFKAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVTLIVPIAMEERLRFAIREGGRTVGAGIVASITE
>Mature_390_residues
AKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETENRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDPELLELVELEIRELLSKYEFPGD
DIPIVKGSALAALEDSNKEIGEDAVRQLMAEVDKYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEVE
IVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGVERGQVLAKPGSVTPHTKFKAEAYILTKEEGGRHTPFF
TNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVTLIVPIAMEERLRFAIREGGRTVGAGIVASITE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=55.8673469387755, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.4663573085847, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.4663573085847, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.185520361991, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.9909706546275, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI194018522, Length=324, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=30.5555555555556, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI54607086, Length=308, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=27.4074074074074, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=32.8358208955224, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=26.984126984127, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729339, Length=240, Percent_Identity=30, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI53729337, Length=240, Percent_Identity=30, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=75.6345177664975, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=75.6345177664975, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=30.7486631016043, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=34.7280334728033, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=268, Percent_Identity=30.5970149253731, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788922, Length=137, Percent_Identity=32.1167883211679, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=52.0100502512563, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=42.9305912596401, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.5555555555556, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.5555555555556, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=26.2921348314607, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=354, Percent_Identity=28.5310734463277, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=442, Percent_Identity=26.2443438914027, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=261, Percent_Identity=30.2681992337165, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=26.3537906137184, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=67.1717171717172, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.2517006802721, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.2517006802721, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=26.4204545454545, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=29.5321637426901, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.8810408921933, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=186, Percent_Identity=31.7204301075269, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=31.2977099236641, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=59.3350383631714, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=59.3350383631714, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=50, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=28.6031042128603, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=28.6031042128603, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=28.6031042128603, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=28.6031042128603, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=28.6666666666667, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=28.6666666666667, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.3768545994065, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31.3333333333333, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=31.1572700296736, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24647046, Length=276, Percent_Identity=26.4492753623188, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647048, Length=276, Percent_Identity=26.4492753623188, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=174, Percent_Identity=35.0574712643678, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=31.7567567567568, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706572, Length=132, Percent_Identity=34.0909090909091, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42794; Mature: 42663

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDPELLELVELEIRELLSKYEFPGDDIPIVKGSALAALEDSNKE
CCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCHH
IGEDAVRQLMAEVDKYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHCCCEECCCEE
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGVERGQVLAKPGSVTPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCC
KFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVTL
CEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEERLRFAIREGGRTVGAGIVASITE
EEEECHHHHHHHHHHHCCCEECCCEEEEECC
>Mature Secondary Structure 
AKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDPELLELVELEIRELLSKYEFPGDDIPIVKGSALAALEDSNKE
CCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCHH
IGEDAVRQLMAEVDKYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHCCCEECCCEE
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGVERGQVLAKPGSVTPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCC
KFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNVTMDVTL
CEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEERLRFAIREGGRTVGAGIVASITE
EEEECHHHHHHHHHHHCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA