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The map label for this gene is trmFO [H]

Identifier: 110634143

GI number: 110634143

Start: 1916819

End: 1918300

Strand: Direct

Name: trmFO [H]

Synonym: Meso_1792

Alternate gene names: 110634143

Gene position: 1916819-1918300 (Clockwise)

Preceding gene: 110634142

Following gene: 110634149

Centisome position: 43.44

GC content: 63.83

Gene sequence:

>1482_bases
TTGTATTCTCCCCACACAGACTTGCAGGCTTTCTCCCGCGTTGCATTGGCGCGCGGTTCGGCCTATCTCCAGCGCATGAC
GACAAAACCCATTCATGTCATTGGCGGCGGGCTCGCGGGTTCGGAAGCGGCTTGGCAGGCGGCGGAGGCAGGCGTGCCGG
TCATCCTCCACGAAATGCGTCCCGTGCGCGGCACCGACGCCCACAAGACGGACGGCCTGGCGGAGCTTGTCTGCTCCAAC
TCATTTCGCTCGGACGATGCGCAGACCAACGCCGTCGGACTGCTGCACGCGGAGATGCGCCTGGCAGGTTCGCTCATCAT
GCGCGCGGGAGATGCCAATCAGGTGCCGGCAGGCGGCGCGCTTGCGGTCGATCGAGACGGCTTCTCGGACGCGGTGACGC
AGGCAATCCACGGCCATCCGCTGATCACGGTGGTGCGCGAGGAAGTGGCCGGTCTCCCGCCCTCCGAATGGGATCAGACG
ATCATCGCTACAGGCCCGCTCACCGCGCCGTCGCTCGCCGAGGCAATCCGCCAGGAGACGGGAGCGGAGGCGCTGGCCTT
CTTCGACGCCATCGCACCGATCGTGCATTTCGACACGATCGACATGGACACCTGCTGGTTCCAGTCGCGCTATGACAAGG
CTGGCCCCGGCGGCACCGGCAAGGATTATATCAACTGCCCCATGAACAAGGAGCAGTACCAAGCTTTCGTACAGGCGCTC
CTCGACGGCGAAAAGACCGCCTTCAAGGAGTGGGAGGGAACGCCCTATTTCGACGGCTGTTTGCCCATCGAGGTAATGGC
CGAGCGCGGGCCCGAGACGCTGCGCCATGGCCCGATGAAGCCCATGGGCCTGACAAACGCACACAAGCCGGACGAAAAGG
CTTATGCCGTCGTGCAGTTGCGGCAGGACAATGCGCTGGGCACGCTCTACAATATGGTCGGCTTCCAGACGAAGCTGAAA
TATGCCGAGCAGGTGCGTATTTTCCGCATGATACCCGGACTGGAAAACGCGGAGTTCGCCCGGCTCGGCGGCCTGCACCG
CAACACCTATATCAACTCCCCCGCCCTGCTCGACATCACGCTGCAGCTGAAATCACGGCCCGGCCTGCGCTTTGCCGGCC
AGATCACCGGTTGCGAGGGCTATGTGGAAAGCGCGGCCATGGGACTCATGGCCGGGCGTTTTGCAGCCGCTGCACGCCTT
GGACGGAAGATGATGCCCCCTCCGGCCACCACGGCCTTCGGCTCGCTCATCAGCCACATTACCGGCGGCCATATCCTGTC
CGAGGACGAGCCGGGCAAGCGCTCCTTCCAGCCGATGAACGTGAATTTCGGACTTTTCCCGCCGGTCGAACTGCCGAAAC
CGGAGGGGAAGCGCCTGCGCGGCAAGGAAAAGACGCTTGCAAAGAAGCGCGCGATCACCGCGCGGGCTCTTGCCGATTGC
AGCGACTGGCTTGGTGTTCCGACCCGCGCGGCTGCCGAATAA

Upstream 100 bases:

>100_bases
CCCGTCTCGACGGATAAACGGCCGGCTTTCTCCTCCTCCCGAAACGCCGGTCGATCAAAACGACACCCGCCGGACCTCCT
CCCCCGGCGGGTGTTGTTTT

Downstream 100 bases:

>100_bases
ACTCTGTTACCAGTCCTGGGCACGAAGCAGGACGGTGCCCTGCAGATTCTGCAGGCGTGAAGATTCGTCGATATCTGCAT
AAATGTAGGAAGGCAGGAAC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MYSPHTDLQAFSRVALARGSAYLQRMTTKPIHVIGGGLAGSEAAWQAAEAGVPVILHEMRPVRGTDAHKTDGLAELVCSN
SFRSDDAQTNAVGLLHAEMRLAGSLIMRAGDANQVPAGGALAVDRDGFSDAVTQAIHGHPLITVVREEVAGLPPSEWDQT
IIATGPLTAPSLAEAIRQETGAEALAFFDAIAPIVHFDTIDMDTCWFQSRYDKAGPGGTGKDYINCPMNKEQYQAFVQAL
LDGEKTAFKEWEGTPYFDGCLPIEVMAERGPETLRHGPMKPMGLTNAHKPDEKAYAVVQLRQDNALGTLYNMVGFQTKLK
YAEQVRIFRMIPGLENAEFARLGGLHRNTYINSPALLDITLQLKSRPGLRFAGQITGCEGYVESAAMGLMAGRFAAAARL
GRKMMPPPATTAFGSLISHITGGHILSEDEPGKRSFQPMNVNFGLFPPVELPKPEGKRLRGKEKTLAKKRAITARALADC
SDWLGVPTRAAAE

Sequences:

>Translated_493_residues
MYSPHTDLQAFSRVALARGSAYLQRMTTKPIHVIGGGLAGSEAAWQAAEAGVPVILHEMRPVRGTDAHKTDGLAELVCSN
SFRSDDAQTNAVGLLHAEMRLAGSLIMRAGDANQVPAGGALAVDRDGFSDAVTQAIHGHPLITVVREEVAGLPPSEWDQT
IIATGPLTAPSLAEAIRQETGAEALAFFDAIAPIVHFDTIDMDTCWFQSRYDKAGPGGTGKDYINCPMNKEQYQAFVQAL
LDGEKTAFKEWEGTPYFDGCLPIEVMAERGPETLRHGPMKPMGLTNAHKPDEKAYAVVQLRQDNALGTLYNMVGFQTKLK
YAEQVRIFRMIPGLENAEFARLGGLHRNTYINSPALLDITLQLKSRPGLRFAGQITGCEGYVESAAMGLMAGRFAAAARL
GRKMMPPPATTAFGSLISHITGGHILSEDEPGKRSFQPMNVNFGLFPPVELPKPEGKRLRGKEKTLAKKRAITARALADC
SDWLGVPTRAAAE
>Mature_493_residues
MYSPHTDLQAFSRVALARGSAYLQRMTTKPIHVIGGGLAGSEAAWQAAEAGVPVILHEMRPVRGTDAHKTDGLAELVCSN
SFRSDDAQTNAVGLLHAEMRLAGSLIMRAGDANQVPAGGALAVDRDGFSDAVTQAIHGHPLITVVREEVAGLPPSEWDQT
IIATGPLTAPSLAEAIRQETGAEALAFFDAIAPIVHFDTIDMDTCWFQSRYDKAGPGGTGKDYINCPMNKEQYQAFVQAL
LDGEKTAFKEWEGTPYFDGCLPIEVMAERGPETLRHGPMKPMGLTNAHKPDEKAYAVVQLRQDNALGTLYNMVGFQTKLK
YAEQVRIFRMIPGLENAEFARLGGLHRNTYINSPALLDITLQLKSRPGLRFAGQITGCEGYVESAAMGLMAGRFAAAARL
GRKMMPPPATTAFGSLISHITGGHILSEDEPGKRSFQPMNVNFGLFPPVELPKPEGKRLRGKEKTLAKKRAITARALADC
SDWLGVPTRAAAE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 53234; Mature: 53234

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSPHTDLQAFSRVALARGSAYLQRMTTKPIHVIGGGLAGSEAAWQAAEAGVPVILHEMR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCC
PVRGTDAHKTDGLAELVCSNSFRSDDAQTNAVGLLHAEMRLAGSLIMRAGDANQVPAGGA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCE
LAVDRDGFSDAVTQAIHGHPLITVVREEVAGLPPSEWDQTIIATGPLTAPSLAEAIRQET
EEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
GAEALAFFDAIAPIVHFDTIDMDTCWFQSRYDKAGPGGTGKDYINCPMNKEQYQAFVQAL
CHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHH
LDGEKTAFKEWEGTPYFDGCLPIEVMAERGPETLRHGPMKPMGLTNAHKPDEKAYAVVQL
HCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
RQDNALGTLYNMVGFQTKLKYAEQVRIFRMIPGLENAEFARLGGLHRNTYINSPALLDIT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEEEEE
LQLKSRPGLRFAGQITGCEGYVESAAMGLMAGRFAAAARLGRKMMPPPATTAFGSLISHI
EEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TGGHILSEDEPGKRSFQPMNVNFGLFPPVELPKPEGKRLRGKEKTLAKKRAITARALADC
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SDWLGVPTRAAAE
HHHCCCCCHHCCC
>Mature Secondary Structure
MYSPHTDLQAFSRVALARGSAYLQRMTTKPIHVIGGGLAGSEAAWQAAEAGVPVILHEMR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCC
PVRGTDAHKTDGLAELVCSNSFRSDDAQTNAVGLLHAEMRLAGSLIMRAGDANQVPAGGA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCE
LAVDRDGFSDAVTQAIHGHPLITVVREEVAGLPPSEWDQTIIATGPLTAPSLAEAIRQET
EEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
GAEALAFFDAIAPIVHFDTIDMDTCWFQSRYDKAGPGGTGKDYINCPMNKEQYQAFVQAL
CHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHH
LDGEKTAFKEWEGTPYFDGCLPIEVMAERGPETLRHGPMKPMGLTNAHKPDEKAYAVVQL
HCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
RQDNALGTLYNMVGFQTKLKYAEQVRIFRMIPGLENAEFARLGGLHRNTYINSPALLDIT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEEEEE
LQLKSRPGLRFAGQITGCEGYVESAAMGLMAGRFAAAARLGRKMMPPPATTAFGSLISHI
EEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TGGHILSEDEPGKRSFQPMNVNFGLFPPVELPKPEGKRLRGKEKTLAKKRAITARALADC
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SDWLGVPTRAAAE
HHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA