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The map label for this gene is ksgA

Identifier: 110634113

GI number: 110634113

Start: 1884195

End: 1885022

Strand: Direct

Name: ksgA

Synonym: Meso_1762

Alternate gene names: 110634113

Gene position: 1884195-1885022 (Clockwise)

Preceding gene: 110634112

Following gene: 110634123

Centisome position: 42.7

GC content: 63.53

Gene sequence:

>828_bases
ATGAGCATGGACGGTCTGCCGCCGCTGCGGGATGTCATCGAGCGGCATGGTCTTTTCGCCAGAAAGGCCTTGGGCCAGAA
TTTTCTTCTCGACCTCAACCTCACGCGCCGCATCGCCCGCACCGCCGGCGGGCTGGACAATGCCACCGTGCTGGAGGTGG
GCCCCGGCCCAGGTGGCCTCACGCGTGCGCTCCTGATGGAAGGAGCAAGGCGCGTTGTGGCGATCGAGCGCGACGAGAGA
TGCATCGCGGCCCTTGAGGAGATCGCCGCGCATTATCCCGGTCGCCTTGAGATCGTCGCCGGCGACGCCATGAAAGCGGA
TTTCGCCGCGCTCGCGGGAAATTCAGGCGACGTAAAAATCGTCGCCAATCTTCCCTACAATATCGGAACCGAGCTTCTTA
TCCGCTGGCTGACACCGCAGACTTGGCCGCCCTTCTACGAATCGATGACGCTCATGTTCCAGCGGGAAGTCGCCGAGCGG
ATCGTCGCGAAGCCCGGAAGCAGTCACTATGGGCGTCTCGGGGTTCTGGCTGGCTGGCGGACCGAAGCCAGGATCGCCTT
TGATGTGCCGCCGCAGGCCTTCACACCACCTCCCAAAGTAATTTCTTCCGTCGTCAAGATCGTCCCGCGGGCAGATCCCC
TGACCGTCGAGGTTGGCCGGCTGGCGCGTACGACCGAAGCCGCCTTCGGGCAGCGGCGAAAAATGCTGCGCCAGAGCCTT
CGATCCGTCGGCGGTGAAGCACTGCTGGAAAAAGCGGGCATCGATGGTACCCGCCGCGCCGAGACCCTCTCGGTGGAGGA
ATTCGTGCGGCTGGCACGGGAGATATGA

Upstream 100 bases:

>100_bases
CCTTCGACATTGCGGGCCGGGGTGTCGCGAAGCCCGATAGCCTGATCGCGGCAATCCGCCTTGCGCGCCTCCTCGCCGAC
AACACTGCGAAGCACAACGC

Downstream 100 bases:

>100_bases
AGTGCAGCGCGGCCTATGGCGAAAAGTCCAGCCCGTGGGTAGCTCTGAAAATTTATCGCCCGACCTTCTTCAGCATTCAG
GCATGGATCCTCGGGCTTGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSMDGLPPLRDVIERHGLFARKALGQNFLLDLNLTRRIARTAGGLDNATVLEVGPGPGGLTRALLMEGARRVVAIERDER
CIAALEEIAAHYPGRLEIVAGDAMKADFAALAGNSGDVKIVANLPYNIGTELLIRWLTPQTWPPFYESMTLMFQREVAER
IVAKPGSSHYGRLGVLAGWRTEARIAFDVPPQAFTPPPKVISSVVKIVPRADPLTVEVGRLARTTEAAFGQRRKMLRQSL
RSVGGEALLEKAGIDGTRRAETLSVEEFVRLAREI

Sequences:

>Translated_275_residues
MSMDGLPPLRDVIERHGLFARKALGQNFLLDLNLTRRIARTAGGLDNATVLEVGPGPGGLTRALLMEGARRVVAIERDER
CIAALEEIAAHYPGRLEIVAGDAMKADFAALAGNSGDVKIVANLPYNIGTELLIRWLTPQTWPPFYESMTLMFQREVAER
IVAKPGSSHYGRLGVLAGWRTEARIAFDVPPQAFTPPPKVISSVVKIVPRADPLTVEVGRLARTTEAAFGQRRKMLRQSL
RSVGGEALLEKAGIDGTRRAETLSVEEFVRLAREI
>Mature_274_residues
SMDGLPPLRDVIERHGLFARKALGQNFLLDLNLTRRIARTAGGLDNATVLEVGPGPGGLTRALLMEGARRVVAIERDERC
IAALEEIAAHYPGRLEIVAGDAMKADFAALAGNSGDVKIVANLPYNIGTELLIRWLTPQTWPPFYESMTLMFQREVAERI
VAKPGSSHYGRLGVLAGWRTEARIAFDVPPQAFTPPPKVISSVVKIVPRADPLTVEVGRLARTTEAAFGQRRKMLRQSLR
SVGGEALLEKAGIDGTRRAETLSVEEFVRLAREI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=290, Percent_Identity=39.3103448275862, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI7657198, Length=237, Percent_Identity=34.5991561181435, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=37.5, Blast_Score=150, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=32.5423728813559, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=34.2465753424658, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=245, Percent_Identity=33.0612244897959, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357273, Length=291, Percent_Identity=34.3642611683849, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=31.8367346938775, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MESSB (Q11HG9)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674321.1
- ProteinModelPortal:   Q11HG9
- SMR:   Q11HG9
- STRING:   Q11HG9
- GeneID:   4181198
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1762
- NMPDR:   fig|266779.1.peg.3703
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q11HG9
- ProtClustDB:   PRK00274
- BioCyc:   MSP266779:MESO_1762-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30070; Mature: 29939

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 76-76 BINDING 102-102 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMDGLPPLRDVIERHGLFARKALGQNFLLDLNLTRRIARTAGGLDNATVLEVGPGPGGL
CCCCCCCHHHHHHHHCCHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCCCCHH
TRALLMEGARRVVAIERDERCIAALEEIAAHYPGRLEIVAGDAMKADFAALAGNSGDVKI
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEE
VANLPYNIGTELLIRWLTPQTWPPFYESMTLMFQREVAERIVAKPGSSHYGRLGVLAGWR
EEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
TEARIAFDVPPQAFTPPPKVISSVVKIVPRADPLTVEVGRLARTTEAAFGQRRKMLRQSL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RSVGGEALLEKAGIDGTRRAETLSVEEFVRLAREI
HHCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SMDGLPPLRDVIERHGLFARKALGQNFLLDLNLTRRIARTAGGLDNATVLEVGPGPGGL
CCCCCCHHHHHHHHCCHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCCCCHH
TRALLMEGARRVVAIERDERCIAALEEIAAHYPGRLEIVAGDAMKADFAALAGNSGDVKI
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEE
VANLPYNIGTELLIRWLTPQTWPPFYESMTLMFQREVAERIVAKPGSSHYGRLGVLAGWR
EEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
TEARIAFDVPPQAFTPPPKVISSVVKIVPRADPLTVEVGRLARTTEAAFGQRRKMLRQSL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RSVGGEALLEKAGIDGTRRAETLSVEEFVRLAREI
HHCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA