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The map label for this gene is prfB [H]

Identifier: 110634059

GI number: 110634059

Start: 1828139

End: 1829107

Strand: Reverse

Name: prfB [H]

Synonym: Meso_1707

Alternate gene names: 110634059

Gene position: 1829107-1828139 (Counterclockwise)

Preceding gene: 110634060

Following gene: 110634058

Centisome position: 41.45

GC content: 61.3

Gene sequence:

>969_bases
ATGCGCGAGCGCCAGTCTCTCGAAGAAAACATCAATGCGATCAATGGTCTCAGCCGATCTCTTGATGACAATGTAGAGCT
GATCAAGCTCGGAGAGGAAGAAGGGGACGCGGGGATCGTCGAGGAGGCGGAATCCGCTATTCGCTCGCTGCGCGGGGAGA
TTTCGGCGCGCCAGATCGAGACGCTTCTTTCCGGAGAGGCCGACGCCAACGACACATATCTGGAGGTTCATGCCGGCGCG
GGCGGCACGGAAAGCCAGGACTGGGCCTTGATGCTGCTTCGGATGTATACGCGCTGGGCGGAGCGGCGGGGCATGAAGGT
CGAGACGCTTGAGGTGCATGACGGTGAGGAGGCCGGCATCAAATCGGCCACCATTCTCGTCCACGGCCACAATGCTTACG
GCTGGCTCAAGACCGAATCGGGCGTGCACCGGCTGGTGCGGATCTCGCCTTTCGACAGCAATGCACGCCGGCACACGTCA
TTCGCGAGCATCTGGGTTTATCCGGTTATCGACGATTCGATCGAGATCGAAATCCCGGAATCAGAGGTGCGGATCGACAC
CTATCGCTCTTCCGGCGCTGGCGGACAGCACGTGAATACGACGGATTCGGCCGTGCGCATTACACACATCCCGACGGGCA
TCGCAGTCGCCTGCCAGCAGGAGCGCTCGCAGCACAAGAACCGGGCGAAGGCGTGGGAGATGCTGCGCGCTCGGCTTTAT
GAGCTGGAGCTCAAGAAGCGCGAGGAGGCGGCGAACGCCACCGAGGCGTCAAAGAGCGACATCGGCTGGGGGCACCAGAT
ACGCTCCTACGTGCTGCAGCCATATCAGCTCGTTAAGGATCTGCGCACCGGTGTGGAGAGTCCTAGCCCGCAGGACGTGC
TGGACGGGGACCTGGACGAATTCATGAAGGCTTCGCTTTCGCAGCGTGTGGGCGGTGTTGAGCCGGCGGAAGTTGCGGAT
ATCGACTGA

Upstream 100 bases:

>100_bases
TATCTTTGACTGGGACGAGGCGGTAAAACGGCTCGAATACCTCAATTCCCGCGCCGAGGACGGCAACCTGTGGAACGATC
CGCAGGAAGCGCAGAAGCTG

Downstream 100 bases:

>100_bases
CCTTACGCACGACGTCTGTGTACCCTCAAAAACCATAATTCCATTTGAGGCCTGTAGCTTGTGCGGTAGTGCTGTATAGC
TGACCGGAGCCGGCGCCGAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRERQSLEENINAINGLSRSLDDNVELIKLGEEEGDAGIVEEAESAIRSLRGEISARQIETLLSGEADANDTYLEVHAGA
GGTESQDWALMLLRMYTRWAERRGMKVETLEVHDGEEAGIKSATILVHGHNAYGWLKTESGVHRLVRISPFDSNARRHTS
FASIWVYPVIDDSIEIEIPESEVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWEMLRARLY
ELELKKREEAANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESPSPQDVLDGDLDEFMKASLSQRVGGVEPAEVAD
ID

Sequences:

>Translated_322_residues
MRERQSLEENINAINGLSRSLDDNVELIKLGEEEGDAGIVEEAESAIRSLRGEISARQIETLLSGEADANDTYLEVHAGA
GGTESQDWALMLLRMYTRWAERRGMKVETLEVHDGEEAGIKSATILVHGHNAYGWLKTESGVHRLVRISPFDSNARRHTS
FASIWVYPVIDDSIEIEIPESEVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWEMLRARLY
ELELKKREEAANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESPSPQDVLDGDLDEFMKASLSQRVGGVEPAEVAD
ID
>Mature_322_residues
MRERQSLEENINAINGLSRSLDDNVELIKLGEEEGDAGIVEEAESAIRSLRGEISARQIETLLSGEADANDTYLEVHAGA
GGTESQDWALMLLRMYTRWAERRGMKVETLEVHDGEEAGIKSATILVHGHNAYGWLKTESGVHRLVRISPFDSNARRHTS
FASIWVYPVIDDSIEIEIPESEVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWEMLRARLY
ELELKKREEAANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESPSPQDVLDGDLDEFMKASLSQRVGGVEPAEVAD
ID

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=38.0622837370242, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=34.375, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=39.2670157068063, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=55.0819672131148, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1787462, Length=239, Percent_Identity=43.9330543933054, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=207, Percent_Identity=37.1980676328502, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=261, Percent_Identity=39.463601532567, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=44.3877551020408, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35804; Mature: 35804

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERQSLEENINAINGLSRSLDDNVELIKLGEEEGDAGIVEEAESAIRSLRGEISARQIE
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLLSGEADANDTYLEVHAGAGGTESQDWALMLLRMYTRWAERRGMKVETLEVHDGEEAGI
HHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
KSATILVHGHNAYGWLKTESGVHRLVRISPFDSNARRHTSFASIWVYPVIDDSIEIEIPE
CEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEECC
SEVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWEMLRARLY
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELELKKREEAANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESPSPQDVLDGDLDE
HHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHH
FMKASLSQRVGGVEPAEVADID
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRERQSLEENINAINGLSRSLDDNVELIKLGEEEGDAGIVEEAESAIRSLRGEISARQIE
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLLSGEADANDTYLEVHAGAGGTESQDWALMLLRMYTRWAERRGMKVETLEVHDGEEAGI
HHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
KSATILVHGHNAYGWLKTESGVHRLVRISPFDSNARRHTSFASIWVYPVIDDSIEIEIPE
CEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEECC
SEVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWEMLRARLY
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELELKKREEAANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESPSPQDVLDGDLDE
HHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHH
FMKASLSQRVGGVEPAEVADID
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA