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The map label for this gene is ilvD2 [H]

Identifier: 110634040

GI number: 110634040

Start: 1804191

End: 1805918

Strand: Reverse

Name: ilvD2 [H]

Synonym: Meso_1688

Alternate gene names: 110634040

Gene position: 1805918-1804191 (Counterclockwise)

Preceding gene: 110634041

Following gene: 110634039

Centisome position: 40.93

GC content: 63.19

Gene sequence:

>1728_bases
ATGATGGACGCTCGTGCGATATCGAAGGAAAAGCTGCCCAGCCGCTACGTTACCGTAGGGCCGGCGCGGGCGCCGCACAG
GTCTTATCTTTACGCGATGGGGCTCTCGGCGGAAGAGATCGCTCAGCCGCTGGTCGGCGTGGCGACGTGCTGGAACGAGG
CTGCGCCCTGCAACATCTCGCTGATGCGCCAGGCGCAAGTGGTGAAAAAGGGCGTCGCGGCAGCCAAAGGCACGCCCCGC
GAGTTCACAACGATTACCGTTACTGATGGCATCGCCATGGGCCACCAGGGCATGAAGGCGTCGCTGGCCTCGCGCGAGGT
AATCGCGGATTCTGTGGAACTCACCATGCGCGGCCATTGCTATGACGCGCTTGTCGGCCTTGCGGGTTGTGACAAATCCC
TGCCGGGGATGATGATGGCCATGGTGCGCCTCAATGTGCCGTCGGTCTTCATCTATGGCGGTTCGATCCTGCCTGGCACC
TGGCGCGGGCGGCAGATCACCGTTCAAGACGTTTTCGAGGCGGTCGGCCAGCATTCGGTTGGCTCCATTTCCGACGACGA
GCTGATGGAAATCGAGCAAGCCGCTTGTCCCTCGGCCGGTTCCTGCGGCGCTCAATTCACGGCCAACACCATGGCGACCG
TTGCCGAGGCGATTGGGCTGGCGCTTCCTTATTCCTGCGGTGCGCCTGCGCCTTACGAGATGCGCGATCGCTTCAACTAT
GCCGCCGGCGAGAAGGTGATGGAACTGGTGGCCAAGCAGATACGGCCGCGTGACATCGTGACCCGGAAGGCGCTTGAAAA
CGCCGCGGCCGTGGTTGCCGCGTCGGGGGGCTCCACCAATGCGGCATTGCATTTGCCGGCCATCGCTCATGAAGCCGGAA
TTGAATTCGACCTTTTCGACGTCGCGGAAGTGTTCAAGAGAACACCCTATGTCGCCGATTTGAAGCCGGGCGGCAAATAT
GTCGCCAAGGACATGTTCGAGGCGGGCGGCATTCCGCTGCTCATGAAGACCTTGCTCGACCATGGCTATCTGCATGGCGA
CTGCATGACCGTCACCGGCCGCACGCTTGCGGAGAACATGGAACGCGTCGAGTGGAACGATGCGCAGGACGTGGTTTATC
CGGCTGACAAGCCGCTGAGCCCGACGGGCGGCGTCGTGGGCCTTAAAGGCAATCTCGCGCCGGATGGGGCAATCGTGAAG
GTTGCAGGCATGAAGAACCTGCGCTTCTCCGGCCCCGCGCGCTGCTTCGATTCGGAAGAGGAATGCTTCGCCGCGGTTTC
CGAGCGCCGCTATAAGGAAGGCGAGGTGCTGGTGATCCGCTACGAAGGTCCGAAAGGCGGGCCGGGCATGCGTGAAATGC
TCGCCACCACCGCGGCCCTTTACGGGCAGGGCATGGGCGACAAGGTGGCGCTGATCACGGACGGGCGGTTTTCAGGCGCG
ACCCGCGGCTTCTGTATCGGTCATGTCGGGCCGGAAGCAGCAGTGGGCGGGCCGATAGGGCTTCTCAATGACGGCGACAT
CATAACCATCGATGCGGTGGCAGGCACGATCGACGTGGCGCTCGGCGAAGCGGAACTGGCCGAACGCCGCGCTGCCTGGA
GGCCGCGCGAAAGCGAATATCGGTCCGGCGCGATCTGGAAATACGCGCAGGGCGTGGGCCCGGCGCGGTATGGCGCGGTG
ACGCATCCCGGCGCCCAGAAAGAAACGCACTGCTATGCGGATATCTGA

Upstream 100 bases:

>100_bases
GCCAATTTCCCGCCATAAAACAGGCGCACCGAGAAATCTCTCGAATGCGCCATCCATAGTTGTGCGTTTGGAGATTGACG
GCCGCGAGGCGGTGAAGGTG

Downstream 100 bases:

>100_bases
GCAAATCATAGGCCGGCTCATCGCCGGGGCCCTCCTGTTGTTCGGGGCGCTCGGTCCCGCGGCTGCGCTTGACGAGAAGG
CCGTCGTGCAGCAGCCCTCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MMDARAISKEKLPSRYVTVGPARAPHRSYLYAMGLSAEEIAQPLVGVATCWNEAAPCNISLMRQAQVVKKGVAAAKGTPR
EFTTITVTDGIAMGHQGMKASLASREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGT
WRGRQITVQDVFEAVGQHSVGSISDDELMEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAPAPYEMRDRFNY
AAGEKVMELVAKQIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHEAGIEFDLFDVAEVFKRTPYVADLKPGGKY
VAKDMFEAGGIPLLMKTLLDHGYLHGDCMTVTGRTLAENMERVEWNDAQDVVYPADKPLSPTGGVVGLKGNLAPDGAIVK
VAGMKNLRFSGPARCFDSEEECFAAVSERRYKEGEVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGA
TRGFCIGHVGPEAAVGGPIGLLNDGDIITIDAVAGTIDVALGEAELAERRAAWRPRESEYRSGAIWKYAQGVGPARYGAV
THPGAQKETHCYADI

Sequences:

>Translated_575_residues
MMDARAISKEKLPSRYVTVGPARAPHRSYLYAMGLSAEEIAQPLVGVATCWNEAAPCNISLMRQAQVVKKGVAAAKGTPR
EFTTITVTDGIAMGHQGMKASLASREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGT
WRGRQITVQDVFEAVGQHSVGSISDDELMEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAPAPYEMRDRFNY
AAGEKVMELVAKQIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHEAGIEFDLFDVAEVFKRTPYVADLKPGGKY
VAKDMFEAGGIPLLMKTLLDHGYLHGDCMTVTGRTLAENMERVEWNDAQDVVYPADKPLSPTGGVVGLKGNLAPDGAIVK
VAGMKNLRFSGPARCFDSEEECFAAVSERRYKEGEVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGA
TRGFCIGHVGPEAAVGGPIGLLNDGDIITIDAVAGTIDVALGEAELAERRAAWRPRESEYRSGAIWKYAQGVGPARYGAV
THPGAQKETHCYADI
>Mature_575_residues
MMDARAISKEKLPSRYVTVGPARAPHRSYLYAMGLSAEEIAQPLVGVATCWNEAAPCNISLMRQAQVVKKGVAAAKGTPR
EFTTITVTDGIAMGHQGMKASLASREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGT
WRGRQITVQDVFEAVGQHSVGSISDDELMEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAPAPYEMRDRFNY
AAGEKVMELVAKQIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHEAGIEFDLFDVAEVFKRTPYVADLKPGGKY
VAKDMFEAGGIPLLMKTLLDHGYLHGDCMTVTGRTLAENMERVEWNDAQDVVYPADKPLSPTGGVVGLKGNLAPDGAIVK
VAGMKNLRFSGPARCFDSEEECFAAVSERRYKEGEVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGA
TRGFCIGHVGPEAAVGGPIGLLNDGDIITIDAVAGTIDVALGEAELAERRAAWRPRESEYRSGAIWKYAQGVGPARYGAV
THPGAQKETHCYADI

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=37.4587458745875, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786464, Length=480, Percent_Identity=36.0416666666667, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI2367371, Length=493, Percent_Identity=35.0912778904665, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788157, Length=508, Percent_Identity=29.1338582677165, Blast_Score=198, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=566, Percent_Identity=41.5194346289753, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60986; Mature: 60986

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDARAISKEKLPSRYVTVGPARAPHRSYLYAMGLSAEEIAQPLVGVATCWNEAAPCNIS
CCCCHHCCHHCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHH
LMRQAQVVKKGVAAAKGTPREFTTITVTDGIAMGHQGMKASLASREVIADSVELTMRGHC
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHCEEEEECCCH
YDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGTWRGRQITVQDVFEAVGQHSV
HHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHHHHHHHHCCCCC
GSISDDELMEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAPAPYEMRDRFNY
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCH
AAGEKVMELVAKQIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHEAGIEFDLFD
HHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCCEEECCHHHHHCCCCEEHHH
VAEVFKRTPYVADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCMTVTGRTLAENM
HHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
ERVEWNDAQDVVYPADKPLSPTGGVVGLKGNLAPDGAIVKVAGMKNLRFSGPARCFDSEE
HHCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
ECFAAVSERRYKEGEVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGA
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
TRGFCIGHVGPEAAVGGPIGLLNDGDIITIDAVAGTIDVALGEAELAERRAAWRPRESEY
CCCEEEECCCCCCCCCCCEEEECCCCEEEEEEECCEEEEEECHHHHHHHHHHCCCCHHHH
RSGAIWKYAQGVGPARYGAVTHPGAQKETHCYADI
CCCCHHHHHCCCCCHHCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MMDARAISKEKLPSRYVTVGPARAPHRSYLYAMGLSAEEIAQPLVGVATCWNEAAPCNIS
CCCCHHCCHHCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHH
LMRQAQVVKKGVAAAKGTPREFTTITVTDGIAMGHQGMKASLASREVIADSVELTMRGHC
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHCEEEEECCCH
YDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGTWRGRQITVQDVFEAVGQHSV
HHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHHHHHHHHCCCCC
GSISDDELMEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAPAPYEMRDRFNY
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCH
AAGEKVMELVAKQIRPRDIVTRKALENAAAVVAASGGSTNAALHLPAIAHEAGIEFDLFD
HHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCCEEECCHHHHHCCCCEEHHH
VAEVFKRTPYVADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCMTVTGRTLAENM
HHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
ERVEWNDAQDVVYPADKPLSPTGGVVGLKGNLAPDGAIVKVAGMKNLRFSGPARCFDSEE
HHCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
ECFAAVSERRYKEGEVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGA
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
TRGFCIGHVGPEAAVGGPIGLLNDGDIITIDAVAGTIDVALGEAELAERRAAWRPRESEY
CCCEEEECCCCCCCCCCCEEEECCCCEEEEEEECCEEEEEECHHHHHHHHHHCCCCHHHH
RSGAIWKYAQGVGPARYGAVTHPGAQKETHCYADI
CCCCHHHHHCCCCCHHCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]