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The map label for this gene is eno

Identifier: 110633984

GI number: 110633984

Start: 1757902

End: 1759176

Strand: Reverse

Name: eno

Synonym: Meso_1632

Alternate gene names: 110633984

Gene position: 1759176-1757902 (Counterclockwise)

Preceding gene: 110633985

Following gene: 110633983

Centisome position: 39.87

GC content: 61.96

Gene sequence:

>1275_bases
ATGACTGCCATTGTCGACATCGTCGGACGTGAAATCCTCGACAGCCGCGGTAACCCCACCGTCGAGGTCGATGTCGTCCT
GGAAGACGGTTCTATAGGCCGCGCGGCGGTACCCTCCGGCGCCTCGACGGGCGCGCATGAGGCTGTGGAATTGCGTGACG
GTGGACCGCGCTACCTCGGTAAAGGCGTTGCGCGGGCGGTTGAAGCGGTCAACGGCGAGATTTTCGAAGCCATCGGCGGC
CTTGAGGCGGAAGACCAGATTCACATCGACCGCACCATGATCGAGCTCGACGGCACCCCCAACAAGAGCAGGCTTGGCGC
CAACGCCATCCTTGGCGTTTCCCTTGCGGTGGCGAAAGCCACTGCGGCTGCTTCCGGCCTGCCGCTCTATCGCTATGTGG
GCGGCGCCTCGGCGCATATCCTGCCGGTGCCGATGATGAATATCCTGAACGGCGGCGCGCATGCGGACAACCCGATCGAT
TTTCAGGAGTTCATGATTATGCCCGTCGGCGCGCCGACGCTGCGCGATGCCGTGCGCTGGGGTTCGGAAATCTTCCACAC
CTTGAAGTCGGGGCTAAAGGCCGCCGGCCACAACACCAATGTGGGTGACGAAGGCGGTTTCGCGCCGAACCTGAAGGACG
CCAATGCCGCGCTCGACTTCATCATGGCCTCCATCGAGAAGGCCGGCTTCAAGCCCGGCGATGAGGTGGCGATCGCGCTC
GACTGCGCCGCGACCGAGTTCTTCAAGGACGGCAAATATGTCTACGAGGGCGAGGGGCAAACGCGCGATGCCCAGGCGCA
GGCGGAGTATCTTTCAAAGCTCGCCGGCGAATACCCCATCATCTCGATCGAAGACGGCATGGCGGAGGACGATTTCGAGG
GATGGAAAGCGTTGACCGATCTTGCCGGAAGCAAGGTGCAGCTCGTGGGAGACGATCTTTTCGTCACGAATGCAGCGCGC
CTCAAAGACGGAATCAATATGGGGATTGCCAACTCGATCCTCGTAAAGGTCAATCAGATCGGCTCGCTGACCGAAACGCT
GGAAGCGGTCGAAACGGCGCATAAAGCGGCCTATACTGCGGTGATGTCGCATCGCTCCGGCGAAACCGAGGATTCCACCA
TCGCCGACCTGGCGGTCGCCACCAATTGCGGGCAGATCAAGACCGGCTCGCTTGCCCGCTCCGACCGGCTCGCCAAATAC
AACCAGCTTATCCGTATCGAAGAACAGCTTGGCCGCGCCGCGCGTTATGCCGGGCGCACCGTGGTGCGGGGCTAA

Upstream 100 bases:

>100_bases
AGCGCTTCCCAAAGCAGCGCAACCGCATTGTCTTTTTTGTCGTGTAGGGTAAGAGGAGCGCAATTTTCCACGCTCCAAGC
CAAGCTAAAAGGACAGCACC

Downstream 100 bases:

>100_bases
AGTTTCACGGCCCGGGGTAAAACATCCGGCGGAAATATTGCGACAGCGAAGGGCTGGACAGGGGTGCGCGGCAGATTGCG
CACCCTTTGTCGTCCGCTAG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAIVDIVGREILDSRGNPTVEVDVVLEDGSIGRAAVPSGASTGAHEAVELRDGGPRYLGKGVARAVEAVNGEIFEAIGG
LEAEDQIHIDRTMIELDGTPNKSRLGANAILGVSLAVAKATAAASGLPLYRYVGGASAHILPVPMMNILNGGAHADNPID
FQEFMIMPVGAPTLRDAVRWGSEIFHTLKSGLKAAGHNTNVGDEGGFAPNLKDANAALDFIMASIEKAGFKPGDEVAIAL
DCAATEFFKDGKYVYEGEGQTRDAQAQAEYLSKLAGEYPIISIEDGMAEDDFEGWKALTDLAGSKVQLVGDDLFVTNAAR
LKDGINMGIANSILVKVNQIGSLTETLEAVETAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEQLGRAARYAGRTVVRG

Sequences:

>Translated_424_residues
MTAIVDIVGREILDSRGNPTVEVDVVLEDGSIGRAAVPSGASTGAHEAVELRDGGPRYLGKGVARAVEAVNGEIFEAIGG
LEAEDQIHIDRTMIELDGTPNKSRLGANAILGVSLAVAKATAAASGLPLYRYVGGASAHILPVPMMNILNGGAHADNPID
FQEFMIMPVGAPTLRDAVRWGSEIFHTLKSGLKAAGHNTNVGDEGGFAPNLKDANAALDFIMASIEKAGFKPGDEVAIAL
DCAATEFFKDGKYVYEGEGQTRDAQAQAEYLSKLAGEYPIISIEDGMAEDDFEGWKALTDLAGSKVQLVGDDLFVTNAAR
LKDGINMGIANSILVKVNQIGSLTETLEAVETAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEQLGRAARYAGRTVVRG
>Mature_423_residues
TAIVDIVGREILDSRGNPTVEVDVVLEDGSIGRAAVPSGASTGAHEAVELRDGGPRYLGKGVARAVEAVNGEIFEAIGGL
EAEDQIHIDRTMIELDGTPNKSRLGANAILGVSLAVAKATAAASGLPLYRYVGGASAHILPVPMMNILNGGAHADNPIDF
QEFMIMPVGAPTLRDAVRWGSEIFHTLKSGLKAAGHNTNVGDEGGFAPNLKDANAALDFIMASIEKAGFKPGDEVAIALD
CAATEFFKDGKYVYEGEGQTRDAQAQAEYLSKLAGEYPIISIEDGMAEDDFEGWKALTDLAGSKVQLVGDDLFVTNAARL
KDGINMGIANSILVKVNQIGSLTETLEAVETAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYN
QLIRIEEQLGRAARYAGRTVVRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.8120649651972, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.6203703703704, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=50.9302325581395, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=50.9302325581395, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.2616822429907, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=60.6976744186046, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=50.1160092807425, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=50.1160092807425, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=174, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=49.5412844036697, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=49.5412844036697, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=49.5412844036697, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=48.2993197278912, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=48.7528344671202, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.3023255813954, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.3023255813954, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.3023255813954, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.3023255813954, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=48.9607390300231, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=48.9607390300231, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_MESSB (Q11HU8)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674192.1
- ProteinModelPortal:   Q11HU8
- SMR:   Q11HU8
- STRING:   Q11HU8
- GeneID:   4179231
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1632
- NMPDR:   fig|266779.1.peg.378
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q11HU8
- ProtClustDB:   PRK00077
- BioCyc:   MSP266779:MESO_1632-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44655; Mature: 44524

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIVGREILDSRGNPTVEVDVVLEDGSIGRAAVPSGASTGAHEAVELRDGGPRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVARAVEAVNGEIFEAIGGLEAEDQIHIDRTMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
TAAASGLPLYRYVGGASAHILPVPMMNILNGGAHADNPIDFQEFMIMPVGAPTLRDAVRW
HHHHCCCCHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHH
GSEIFHTLKSGLKAAGHNTNVGDEGGFAPNLKDANAALDFIMASIEKAGFKPGDEVAIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
DCAATEFFKDGKYVYEGEGQTRDAQAQAEYLSKLAGEYPIISIEDGMAEDDFEGWKALTD
EHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
LAGSKVQLVGDDLFVTNAARLKDGINMGIANSILVKVNQIGSLTETLEAVETAHKAAYTA
HCCCEEEEECCCEEEECHHHHHCCCCCCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLGRAARYAGRT
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVRG
CCCC
>Mature Secondary Structure 
TAIVDIVGREILDSRGNPTVEVDVVLEDGSIGRAAVPSGASTGAHEAVELRDGGPRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVARAVEAVNGEIFEAIGGLEAEDQIHIDRTMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
TAAASGLPLYRYVGGASAHILPVPMMNILNGGAHADNPIDFQEFMIMPVGAPTLRDAVRW
HHHHCCCCHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHH
GSEIFHTLKSGLKAAGHNTNVGDEGGFAPNLKDANAALDFIMASIEKAGFKPGDEVAIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
DCAATEFFKDGKYVYEGEGQTRDAQAQAEYLSKLAGEYPIISIEDGMAEDDFEGWKALTD
EHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
LAGSKVQLVGDDLFVTNAARLKDGINMGIANSILVKVNQIGSLTETLEAVETAHKAAYTA
HCCCEEEEECCCEEEECHHHHHCCCCCCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLGRAARYAGRT
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA