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The map label for this gene is pdhC [H]

Identifier: 110633980

GI number: 110633980

Start: 1753060

End: 1754418

Strand: Reverse

Name: pdhC [H]

Synonym: Meso_1628

Alternate gene names: 110633980

Gene position: 1754418-1753060 (Counterclockwise)

Preceding gene: 110633981

Following gene: 110633979

Centisome position: 39.76

GC content: 63.13

Gene sequence:

>1359_bases
ATGCCGATCCAGATCACCATGCCGGCCCTCTCGCCCACCATGGAAGAGGGCAATCTTGCCAAGTGGCTGGTTAAGGAGGG
GGACAGCGTTTCGCCCGGCGATGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTGGAAGCCGTTGACGAGG
GCACCGTGGCCAAGATCGTCGTGCCCGAGGGCACACAGGGCGTGAAGGTGAATGCGCTGATCGCCATTCTCGCCGGTGAG
GGTGAGGACGCGGCTCAAGCGGCGAAGGCGAGCGGAAACGGTGGCGCGGCGGCGGCTCCCGAACCCAAACCGGAGGCAAA
GCCCGAGGCGACGCCTTCCGCGTCTAAGCAGCCGGAAGAAGCGGAGAACCGGCCTGCGCCTGCCGCTAAACCGGCCGCCC
CACAACCCTCCGCCGATAAGGACGGCCGCGTTTTTGCCTCGCCGCTTGCCCGCCGCATCGCCAAGGATGCCGGCATTGAT
CTGTCGGCCATATCCGGTTCCGGCCCGCGCGGACGCGTGGTGAGAGCGGATGTGGAGGCTGCGATCAGCGGCGGAACTGC
AAAGCCTGCCGCGGCGGCGCCCGAAGGGCCAGCTCCGGCTCCCAAGCCCATGTCGGACGAAGCGGTGCTTAAACTGTTCG
AGGAAGGCTCTTACGAGCTCGTGCCGCATGACGGCATGCGCAAGACCATCGCGCGCAGGCTGGTCGAGGCGAAAAGCACC
ATCCCGCATTTTTATCTCACGCTCGACTGCGAGATCGATGCCCTTCTGGCGCTCCGCAAGCAGCTCAACGACGCCGCGCC
CATGGTGAAGGCGGAGGCCGGCGAAAAGCCCGCATACAAGCTCTCGGTCAACGACCTGATCATCAAGGCTTGGGCGTTGG
CGCTGAAAGCGGTGCCGGAGGCAAATGCGTCCTGGACCGAGAGCGCGATGATCAAGCACAAGCACGCCGATGTCGGGGTC
GCGGTCTCTATTCCCGGAGGGTTGATCACGCCCATTGTAAAAAGGGCCGATGAGAAGACGCTGTCAGTTATCTCCAACGA
GATGAAGGATTTGGCCGCTCGCGCCCGCAACCGCAAGCTCAAACCGGAAGAGTATCAGGGGGGCACATCGGCCATTTCCA
ACCTGGGCATGTTCGGCATCAAGGATTTCGCGGCAGTGATCAATCCGCCACATGCGACAATCCTTGCGATCGGCGCGGGC
GAGGAGCGGCCTGTGGTCAGGAACGGAGAGATCAAGATTGCCACGGTGATGTCAGTCACGCTCTCGACAGACCACCGCGC
GGTGGATGGGGCGCTCGGAGCTGAACTGCTTACCGCCTTCAAACGGCTGATTGAGAATCCCTTCGGAATGCTCGTCTAG

Upstream 100 bases:

>100_bases
CCCTATGCGGCCAATCTGGAGAAGCTCGCTTTGCCGAGCGTGGTGGAAGTGGTCGAGGCGGTGAAAGCCGTCACCTACCG
GGCCTGAGGAGGAGTAAGCC

Downstream 100 bases:

>100_bases
GTCTTTGCCTGCCGTTCTTCGGGAATGCGGGGTCTGGAGGAGATAACGAGGTTGCTCGTATGAAGACCATCCTTTGTTAC
GGTGACTCCCTCACTTGGGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPIQITMPALSPTMEEGNLAKWLVKEGDSVSPGDVIAEIETDKATMEVEAVDEGTVAKIVVPEGTQGVKVNALIAILAGE
GEDAAQAAKASGNGGAAAAPEPKPEAKPEATPSASKQPEEAENRPAPAAKPAAPQPSADKDGRVFASPLARRIAKDAGID
LSAISGSGPRGRVVRADVEAAISGGTAKPAAAAPEGPAPAPKPMSDEAVLKLFEEGSYELVPHDGMRKTIARRLVEAKST
IPHFYLTLDCEIDALLALRKQLNDAAPMVKAEAGEKPAYKLSVNDLIIKAWALALKAVPEANASWTESAMIKHKHADVGV
AVSIPGGLITPIVKRADEKTLSVISNEMKDLAARARNRKLKPEEYQGGTSAISNLGMFGIKDFAAVINPPHATILAIGAG
EERPVVRNGEIKIATVMSVTLSTDHRAVDGALGAELLTAFKRLIENPFGMLV

Sequences:

>Translated_452_residues
MPIQITMPALSPTMEEGNLAKWLVKEGDSVSPGDVIAEIETDKATMEVEAVDEGTVAKIVVPEGTQGVKVNALIAILAGE
GEDAAQAAKASGNGGAAAAPEPKPEAKPEATPSASKQPEEAENRPAPAAKPAAPQPSADKDGRVFASPLARRIAKDAGID
LSAISGSGPRGRVVRADVEAAISGGTAKPAAAAPEGPAPAPKPMSDEAVLKLFEEGSYELVPHDGMRKTIARRLVEAKST
IPHFYLTLDCEIDALLALRKQLNDAAPMVKAEAGEKPAYKLSVNDLIIKAWALALKAVPEANASWTESAMIKHKHADVGV
AVSIPGGLITPIVKRADEKTLSVISNEMKDLAARARNRKLKPEEYQGGTSAISNLGMFGIKDFAAVINPPHATILAIGAG
EERPVVRNGEIKIATVMSVTLSTDHRAVDGALGAELLTAFKRLIENPFGMLV
>Mature_451_residues
PIQITMPALSPTMEEGNLAKWLVKEGDSVSPGDVIAEIETDKATMEVEAVDEGTVAKIVVPEGTQGVKVNALIAILAGEG
EDAAQAAKASGNGGAAAAPEPKPEAKPEATPSASKQPEEAENRPAPAAKPAAPQPSADKDGRVFASPLARRIAKDAGIDL
SAISGSGPRGRVVRADVEAAISGGTAKPAAAAPEGPAPAPKPMSDEAVLKLFEEGSYELVPHDGMRKTIARRLVEAKSTI
PHFYLTLDCEIDALLALRKQLNDAAPMVKAEAGEKPAYKLSVNDLIIKAWALALKAVPEANASWTESAMIKHKHADVGVA
VSIPGGLITPIVKRADEKTLSVISNEMKDLAARARNRKLKPEEYQGGTSAISNLGMFGIKDFAAVINPPHATILAIGAGE
ERPVVRNGEIKIATVMSVTLSTDHRAVDGALGAELLTAFKRLIENPFGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=457, Percent_Identity=43.1072210065646, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI203098753, Length=481, Percent_Identity=37.6299376299376, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI203098816, Length=477, Percent_Identity=37.5262054507338, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=43.9490445859873, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI110671329, Length=464, Percent_Identity=27.1551724137931, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=31.8777292576419, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786946, Length=450, Percent_Identity=30.2222222222222, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=31.8604651162791, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=44.9339207048458, Blast_Score=340, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=34.9358974358974, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=466, Percent_Identity=28.3261802575107, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=458, Percent_Identity=29.9126637554585, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=472, Percent_Identity=43.8559322033898, Blast_Score=315, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6320352, Length=453, Percent_Identity=29.1390728476821, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321632, Length=181, Percent_Identity=34.2541436464088, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129315, Length=456, Percent_Identity=39.9122807017544, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24582497, Length=445, Percent_Identity=39.3258426966292, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=28.2894736842105, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=31.6017316017316, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47129; Mature: 46998

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQITMPALSPTMEEGNLAKWLVKEGDSVSPGDVIAEIETDKATMEVEAVDEGTVAKIV
CCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQGVKVNALIAILAGEGEDAAQAAKASGNGGAAAAPEPKPEAKPEATPSASKQPEE
ECCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AENRPAPAAKPAAPQPSADKDGRVFASPLARRIAKDAGIDLSAISGSGPRGRVVRADVEA
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECHHH
AISGGTAKPAAAAPEGPAPAPKPMSDEAVLKLFEEGSYELVPHDGMRKTIARRLVEAKST
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHC
IPHFYLTLDCEIDALLALRKQLNDAAPMVKAEAGEKPAYKLSVNDLIIKAWALALKAVPE
CCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
ANASWTESAMIKHKHADVGVAVSIPGGLITPIVKRADEKTLSVISNEMKDLAARARNRKL
CCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
KPEEYQGGTSAISNLGMFGIKDFAAVINPPHATILAIGAGEERPVVRNGEIKIATVMSVT
CCHHHCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCEEEEEEEEEE
LSTDHRAVDGALGAELLTAFKRLIENPFGMLV
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PIQITMPALSPTMEEGNLAKWLVKEGDSVSPGDVIAEIETDKATMEVEAVDEGTVAKIV
CEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQGVKVNALIAILAGEGEDAAQAAKASGNGGAAAAPEPKPEAKPEATPSASKQPEE
ECCCCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AENRPAPAAKPAAPQPSADKDGRVFASPLARRIAKDAGIDLSAISGSGPRGRVVRADVEA
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECHHH
AISGGTAKPAAAAPEGPAPAPKPMSDEAVLKLFEEGSYELVPHDGMRKTIARRLVEAKST
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHC
IPHFYLTLDCEIDALLALRKQLNDAAPMVKAEAGEKPAYKLSVNDLIIKAWALALKAVPE
CCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
ANASWTESAMIKHKHADVGVAVSIPGGLITPIVKRADEKTLSVISNEMKDLAARARNRKL
CCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
KPEEYQGGTSAISNLGMFGIKDFAAVINPPHATILAIGAGEERPVVRNGEIKIATVMSVT
CCHHHCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCEEEEEEEEEE
LSTDHRAVDGALGAELLTAFKRLIENPFGMLV
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]