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The map label for this gene is glnA [H]

Identifier: 110633897

GI number: 110633897

Start: 1662600

End: 1664009

Strand: Reverse

Name: glnA [H]

Synonym: Meso_1544

Alternate gene names: 110633897

Gene position: 1664009-1662600 (Counterclockwise)

Preceding gene: 110633898

Following gene: 110633892

Centisome position: 37.71

GC content: 58.3

Gene sequence:

>1410_bases
ATGACCACAGCACAGGATATTCTGAAGCAGATCAAGGACAACGACGTTAAGTTCGTTGACCTGCGCTTTACGGATCCGAA
GGGCAAACTACAGCATGTGACGATGGACGTCGTCGCCGTAGACGAGGACATGTTCGCCGACGGTGTCATGTTCGACGGCT
CATCGATCGGCGGCTGGAAGGCCATCAACGAATCCGACATGGTGCTGATGCCAGATCCTGAGACGGCTCATGTGGATCCC
TTCTTCGCCCAGTCCACAATGGTGGTCTACTGCGATATTCTGGACCCGATCTCCGGGGAAGCCTATAACCGTGATCCGCG
CGGCATTGCCAAAAAGGCCGAGGCCTATTTGCGCGCAGAAGGCATCGGGGACACCGTCTTCATAGGTCCGGAAGCCGAGT
TCTTCGTGTTCGATGACGTGAAGTACAAGGCTGATCCCTACAATACCGGCTTCAAGCTCGACTCCTCGGAGCTGCCGTCC
AACGACGATACCGATTACGAGACGGGAAACTTGGGCCATCGGCCGCGTGTTAAGGGCGGGTACTTCCCGGTACCGCCTGT
CGACTCTCTGCAGGATATGCGCTCAGAGATGCTCTCTGTGCTGACCGAGATGGGTGTGGTCGTTGAGAAGCACCATCACG
AGGTGGCCGCAGCCCAGCATGAGCTTGGCCTCAAGTTCGACACGCTGGTGCGCAACGCCGACAAGATGCAGATCTACAAG
TATGTTGTGCACCAGGTTGCAAATGCCTACGGCAAAACGGCCACCTTCATGCCGAAGCCCATCTATGGCGATAACGGCTC
GGGCATGCATGTGCACTTGTCGATCTGGAAGGACGGCAAGCCCACCTTCGCCGGCAACGAGTATGCCGGACTTTCCGAGT
CCTGCCTCTACTTCATCGGCGGCATTATCAAGCATGCCAAGGCGATCAACGCCTTCACCAACCCGACCACCAACTCCTAC
AAGCGTCTGGTGCCGGGCTTCGAGGCGCCGGTTCTGCTTGCCTATTCGGCCCGCAACCGCTCGGCCTCCTGCCGTATCCC
CTTCGGCACTTCGCCCAAGTCGAAGCGCGTCGAGGTCCGGTTCCCTGACCCGACGGCGAACCCCTATCTCTGCTTCTCGG
CCCTGCTGATGGCCGGTCTGGACGGCATTAAGAACAAGATCCATCCGGGCCAGGCCATGGACAAGGATCTTTATGACCTG
CCGCCGAAGGAGCTGAAGAAGGTCCCGACTGTCTGCGGCAGCCTGCGCGAAGCGCTGATGAGCCTCGACAAGGATCGCGG
CTTCCTCAAAGCCGGCGGCGTCTTCGATGACGACCAGATCGATGCCTATATCGAGCTCAAGATGGCTGAGGTGCTGCGCT
TTGACATGACCCCGCATCCGGTCGAATTCGACATGTACTACTCGGTGTAA

Upstream 100 bases:

>100_bases
AGTCATCCGCATCCGCACCGGCGAAACCGGGCCTGATGCGGTCTGAACCGGTCCTTTGGCCGATCGCAAGAGAATTGTCA
TTTACAGGGGTTGAAAAGGT

Downstream 100 bases:

>100_bases
GCAAACTCGCAGATCATAACGATGAAGCCCCCGGACAACCGGGGGCTTTTTTTAATTCAATCGCCGCGACCGAAGTGCCG
TTACGGCACGCCGGGAGCGC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTAQDILKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVAVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDPETAHVDP
FFAQSTMVVYCDILDPISGEAYNRDPRGIAKKAEAYLRAEGIGDTVFIGPEAEFFVFDDVKYKADPYNTGFKLDSSELPS
NDDTDYETGNLGHRPRVKGGYFPVPPVDSLQDMRSEMLSVLTEMGVVVEKHHHEVAAAQHELGLKFDTLVRNADKMQIYK
YVVHQVANAYGKTATFMPKPIYGDNGSGMHVHLSIWKDGKPTFAGNEYAGLSESCLYFIGGIIKHAKAINAFTNPTTNSY
KRLVPGFEAPVLLAYSARNRSASCRIPFGTSPKSKRVEVRFPDPTANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDL
PPKELKKVPTVCGSLREALMSLDKDRGFLKAGGVFDDDQIDAYIELKMAEVLRFDMTPHPVEFDMYYSV

Sequences:

>Translated_469_residues
MTTAQDILKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVAVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDPETAHVDP
FFAQSTMVVYCDILDPISGEAYNRDPRGIAKKAEAYLRAEGIGDTVFIGPEAEFFVFDDVKYKADPYNTGFKLDSSELPS
NDDTDYETGNLGHRPRVKGGYFPVPPVDSLQDMRSEMLSVLTEMGVVVEKHHHEVAAAQHELGLKFDTLVRNADKMQIYK
YVVHQVANAYGKTATFMPKPIYGDNGSGMHVHLSIWKDGKPTFAGNEYAGLSESCLYFIGGIIKHAKAINAFTNPTTNSY
KRLVPGFEAPVLLAYSARNRSASCRIPFGTSPKSKRVEVRFPDPTANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDL
PPKELKKVPTVCGSLREALMSLDKDRGFLKAGGVFDDDQIDAYIELKMAEVLRFDMTPHPVEFDMYYSV
>Mature_468_residues
TTAQDILKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVAVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDPETAHVDPF
FAQSTMVVYCDILDPISGEAYNRDPRGIAKKAEAYLRAEGIGDTVFIGPEAEFFVFDDVKYKADPYNTGFKLDSSELPSN
DDTDYETGNLGHRPRVKGGYFPVPPVDSLQDMRSEMLSVLTEMGVVVEKHHHEVAAAQHELGLKFDTLVRNADKMQIYKY
VVHQVANAYGKTATFMPKPIYGDNGSGMHVHLSIWKDGKPTFAGNEYAGLSESCLYFIGGIIKHAKAINAFTNPTTNSYK
RLVPGFEAPVLLAYSARNRSASCRIPFGTSPKSKRVEVRFPDPTANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLP
PKELKKVPTVCGSLREALMSLDKDRGFLKAGGVFDDDQIDAYIELKMAEVLRFDMTPHPVEFDMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=66.025641025641, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=315, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52126; Mature: 51995

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAQDILKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVAVDEDMFADGVMFDGSSIGGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCHHCCCCEEECCCCCCCCE
AINESDMVLMPDPETAHVDPFFAQSTMVVYCDILDPISGEAYNRDPRGIAKKAEAYLRAE
ECCCCCEEEECCCCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GIGDTVFIGPEAEFFVFDDVKYKADPYNTGFKLDSSELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSLQDMRSEMLSVLTEMGVVVEKHHHEVAAAQHELGLKFDTLVRNADKMQIYK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
YVVHQVANAYGKTATFMPKPIYGDNGSGMHVHLSIWKDGKPTFAGNEYAGLSESCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
GIIKHAKAINAFTNPTTNSYKRLVPGFEAPVLLAYSARNRSASCRIPFGTSPKSKRVEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDPTANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLPPKELKKVPTVCGSLREALM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRGFLKAGGVFDDDQIDAYIELKMAEVLRFDMTPHPVEFDMYYSV
HHHCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEEEEEC
>Mature Secondary Structure 
TTAQDILKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVAVDEDMFADGVMFDGSSIGGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCHHCCCCEEECCCCCCCCE
AINESDMVLMPDPETAHVDPFFAQSTMVVYCDILDPISGEAYNRDPRGIAKKAEAYLRAE
ECCCCCEEEECCCCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GIGDTVFIGPEAEFFVFDDVKYKADPYNTGFKLDSSELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSLQDMRSEMLSVLTEMGVVVEKHHHEVAAAQHELGLKFDTLVRNADKMQIYK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
YVVHQVANAYGKTATFMPKPIYGDNGSGMHVHLSIWKDGKPTFAGNEYAGLSESCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
GIIKHAKAINAFTNPTTNSYKRLVPGFEAPVLLAYSARNRSASCRIPFGTSPKSKRVEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDPTANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLPPKELKKVPTVCGSLREALM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRGFLKAGGVFDDDQIDAYIELKMAEVLRFDMTPHPVEFDMYYSV
HHHCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2882467 [H]