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The map label for this gene is otsB [H]

Identifier: 110633882

GI number: 110633882

Start: 1647105

End: 1647881

Strand: Reverse

Name: otsB [H]

Synonym: Meso_1529

Alternate gene names: 110633882

Gene position: 1647881-1647105 (Counterclockwise)

Preceding gene: 110633884

Following gene: 110633881

Centisome position: 37.35

GC content: 59.85

Gene sequence:

>777_bases
ATGGCCGGCGAAGCAGAACCTCTTTCATCACTCGATTGTTCGGCACTGAAACCGAACAGCATAGCGATCTTCCTCGATTT
CGACGGGACCCTTGCGGATATCGCCGAACGCCCGGACGAAGTTTCCGTTCCCCCAACCACGCGCGAGGCGCTCGCGCGCC
TTCTGAAGAGCACCGACGGCGCAATTGCCATTATCACCGGCCGGCCGATCGAGGTCATCGATCATTTTCTGGCGCCTCTC
CTCCTGCCGGTGGCCGGTGTGCATGGGATGGAGCGCAGGACCGCCGGCGGACAACATGTCGGGAGCGTAATTGATGAGGA
GGAGCTTTCGGCAGTGCGCGCTAAACTGCAGGCATTCGCCGACTCGCATGAGGGGCTTCTGCTGGAGTTAAAGCACGGTT
CGCTGGCTCTTCACTATCGGCAGCGACCCGACCTCGAAGCCGCCTCGATAGCAGCCGTGCACGCGGCGGCGAACGACTCA
AAGGGGCTTCATCTCCTGCATGGCAAGATGGTGCTTGAGGTGAAGGGCGGGAAGGCAACGAAGGCCGATGCTCTGACCGC
CTTTATGCAGGAGGACCCTTTTCGCGGACGCACACCCCTTTTTGCGGGGGATGACGTCACCGACGAGGATGCCTTCAACC
AGATCGCGCATATAAACGGGTTCTCCATAAAGATCGGAGGCGGTAAAACATCTGCCCGATTTAGGGCTGATGAAGCCAGC
AGTTTTCGCGAATGGCTCGAACGCCTGGCCGCGAGGTTCGAGTCCCGGGACTATTGA

Upstream 100 bases:

>100_bases
TATCTGCCTCCCAGCAGGGCTGCTGGAATTACGCTGCAGCATCCAAAATGCGGTCACCATGGCAGGAACATGACCGCTTC
ACCCACACTATCGTGAAGTC

Downstream 100 bases:

>100_bases
GGATAGAGCTATATCATGACGACACTTAATCTCGGAATTATCGGTAATTGCAGCTTTTCCGCCCTGGTCGATCCACAAGG
CAGGATCGTGTGGTGTTGTC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAGEAEPLSSLDCSALKPNSIAIFLDFDGTLADIAERPDEVSVPPTTREALARLLKSTDGAIAIITGRPIEVIDHFLAPL
LLPVAGVHGMERRTAGGQHVGSVIDEEELSAVRAKLQAFADSHEGLLLELKHGSLALHYRQRPDLEAASIAAVHAAANDS
KGLHLLHGKMVLEVKGGKATKADALTAFMQEDPFRGRTPLFAGDDVTDEDAFNQIAHINGFSIKIGGGKTSARFRADEAS
SFREWLERLAARFESRDY

Sequences:

>Translated_258_residues
MAGEAEPLSSLDCSALKPNSIAIFLDFDGTLADIAERPDEVSVPPTTREALARLLKSTDGAIAIITGRPIEVIDHFLAPL
LLPVAGVHGMERRTAGGQHVGSVIDEEELSAVRAKLQAFADSHEGLLLELKHGSLALHYRQRPDLEAASIAAVHAAANDS
KGLHLLHGKMVLEVKGGKATKADALTAFMQEDPFRGRTPLFAGDDVTDEDAFNQIAHINGFSIKIGGGKTSARFRADEAS
SFREWLERLAARFESRDY
>Mature_257_residues
AGEAEPLSSLDCSALKPNSIAIFLDFDGTLADIAERPDEVSVPPTTREALARLLKSTDGAIAIITGRPIEVIDHFLAPLL
LPVAGVHGMERRTAGGQHVGSVIDEEELSAVRAKLQAFADSHEGLLLELKHGSLALHYRQRPDLEAASIAAVHAAANDSK
GLHLLHGKMVLEVKGGKATKADALTAFMQEDPFRGRTPLFAGDDVTDEDAFNQIAHINGFSIKIGGGKTSARFRADEASS
FREWLERLAARFESRDY

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=239, Percent_Identity=37.6569037656904, Blast_Score=160, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920676, Length=257, Percent_Identity=26.8482490272374, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27772; Mature: 27640

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEAEPLSSLDCSALKPNSIAIFLDFDGTLADIAERPDEVSVPPTTREALARLLKSTDG
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
AIAIITGRPIEVIDHFLAPLLLPVAGVHGMERRTAGGQHVGSVIDEEELSAVRAKLQAFA
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCHHHHHHHHHHHHHHH
DSHEGLLLELKHGSLALHYRQRPDLEAASIAAVHAAANDSKGLHLLHGKMVLEVKGGKAT
CCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCC
KADALTAFMQEDPFRGRTPLFAGDDVTDEDAFNQIAHINGFSIKIGGGKTSARFRADEAS
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECHHHH
SFREWLERLAARFESRDY
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGEAEPLSSLDCSALKPNSIAIFLDFDGTLADIAERPDEVSVPPTTREALARLLKSTDG
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
AIAIITGRPIEVIDHFLAPLLLPVAGVHGMERRTAGGQHVGSVIDEEELSAVRAKLQAFA
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCHHHHHHHHHHHHHHH
DSHEGLLLELKHGSLALHYRQRPDLEAASIAAVHAAANDSKGLHLLHGKMVLEVKGGKAT
CCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCC
KADALTAFMQEDPFRGRTPLFAGDDVTDEDAFNQIAHINGFSIKIGGGKTSARFRADEAS
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECHHHH
SFREWLERLAARFESRDY
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]