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The map label for this gene is ilvI [H]

Identifier: 110633841

GI number: 110633841

Start: 1585032

End: 1586834

Strand: Direct

Name: ilvI [H]

Synonym: Meso_1488

Alternate gene names: 110633841

Gene position: 1585032-1586834 (Clockwise)

Preceding gene: 110633840

Following gene: 110633842

Centisome position: 35.92

GC content: 62.67

Gene sequence:

>1803_bases
ATGGAACAGCGGGCGCCGGAGCCAAGGGCACACGAAACGGGCAGACAGGAAATGACAGGCGCGGAGATGGTCGTGCAGGC
GCTGAAGGACAACGGCGTCGAGCACATCTTCGGCTATCCTGGCGGCGCGGTGCTCCCCATTTATGATGAGCTCTTCCAGC
AGGACCAGATACAGCACATTCTCGTGCGCCATGAGCAAGGCGCAGGGCACGCCGCGGAAGGTTATGCGCGGTCGACCGGC
AAGGCCGGCGTGATGCTGGTGACCTCCGGTCCCGGCGCGACCAACGCCGTCACGCCGCTTCAGGACGCGTTGATGGATTC
CATCCCGCTTGTCTGCATCACCGGCCAGGTGCCGACGCCGCTCATCGGCTCCGACGCTTTCCAGGAATGCGATACGGTGG
GCATCACGCGCCCATGCACCAAACACAATTGGCTTGTGCGGGACGTCAGCGATCTCGCGGCTATTCTGCATGAGGCTTTC
CATGTCGCCACCACGGGCCGGCCCGGCCCGGTCGTGGTCGACATCCCCAAGGACGTGCAATTTGCGCGTGGCATCTACAC
CCCGCCGCAGACCGCTCCGCGGACAAGTTACCAGCCGACGCTCGACGGAGATCAGGAAAAGATCCGTGCGGCCGTCGCGC
TGATGGCTTCCGCCCGCCGGCCGATCATCTATACCGGCGGCGGCGTCATCAATGCCGGACCGGAGGCGAGCCATCTTCTG
CGCGAACTGGTGGGGCTGACGGGTTTCCCGATTACGTCGACGCTGATGGGCCTTGGCGCCTACCCGGCCTCCGGCGAGAA
CTGGCTCGGCATGCTCGGCATGCACGGGACCTATGAAGCCAATATGGCCATGCATGACTGCGACGTGATGCTATGCATCG
GCGCACGGTTCGACGACCGCATCACCGGCCGGCTCGATGCCTTCTCGCCGAACTCGCGCAAGATCCATGTCGATGTGGAC
CCTTCATCGATCAATAAGAATGTGCGGGTCGATATTCCCATCATCGGCGATGCTGGCCGGGTGCTTGAGGACATGGTGCG
GCTCTGGCGGGCGAGCGCCAATGCCGACAAGGCGGCGCTCAAGCCTTGGTGGGAGCAGATCAACAGGTGGCGCGCGCGCC
AGTCGCTGGCCTATAAAACCAACAGCGACGTGATCATGCCGCAATACGCGGTCGAGCGGCTTTATGAACTGACGAAGGGG
CGCGACGTCTACGTCACCACCGAGGTGGGCCAACACCAGATGTGGGCGGCGCAGCACTTCGGCTTCGATCAGCCCAATCG
GTGGATGACCTCTGGCGGCCTCGGGACAATGGGATATGGGCTGCCGGCCGCGCTGGGGGTGCAGATCGCGCATCCGGACG
CGTTGGTCATCGACATCGCCGGCGACGCCTCCGTCCTGATGACCATGCAGGAAATGAGCTCGGCGGTGCAGCATGACGCG
CCGATCAAGATCTTTATCCTCAATAACCAGTATATGGGGATGGTGCGGCAGTGGCAGCAGCTTTTGCATGGAAACCGGCT
GTCGCATTCCTATACCGAAGCATTGCCTGATTTCGTGAAGCTGGCTGAAGCCTACGGGTGTCACGGTATTCGCTGCGAGA
AACCGGGCGAACTCGACGACGCGATCCGCGAGATGATCGATGTGAAGAAGCCGGTGATCTTCGATTGCCGGGTTGCGAAT
CTCGCCAACTGCTTCCCGATGATCCCTTCCGGCAAGGCGCATAACGAGATGCTGCTGCCGGACGAGGCAACCGACGAGGC
CGTGGCGAATGCAATCGATGCCGAGGGCAGGGCGCTGGTGTAG

Upstream 100 bases:

>100_bases
GCGTAGCCGCCAGGCGACAGCCGCTCATGTGCAGAAGACAGGCTCCCTCGGGGGCCTTTTTTATTGGGCCGAAGACGACG
ATCAGAACGGGAAAAGAGCG

Downstream 100 bases:

>100_bases
ACGGAAAGACAAAGCAGAAGAATTTGCAGCGAGAGATATTATGAACGCCCAACTTCAGCCGACCGGTTCAGCCTACTTCA
TCACCAAGGAAACGGAAAAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MEQRAPEPRAHETGRQEMTGAEMVVQALKDNGVEHIFGYPGGAVLPIYDELFQQDQIQHILVRHEQGAGHAAEGYARSTG
KAGVMLVTSGPGATNAVTPLQDALMDSIPLVCITGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVRDVSDLAAILHEAF
HVATTGRPGPVVVDIPKDVQFARGIYTPPQTAPRTSYQPTLDGDQEKIRAAVALMASARRPIIYTGGGVINAGPEASHLL
RELVGLTGFPITSTLMGLGAYPASGENWLGMLGMHGTYEANMAMHDCDVMLCIGARFDDRITGRLDAFSPNSRKIHVDVD
PSSINKNVRVDIPIIGDAGRVLEDMVRLWRASANADKAALKPWWEQINRWRARQSLAYKTNSDVIMPQYAVERLYELTKG
RDVYVTTEVGQHQMWAAQHFGFDQPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVLMTMQEMSSAVQHDA
PIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALPDFVKLAEAYGCHGIRCEKPGELDDAIREMIDVKKPVIFDCRVAN
LANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAEGRALV

Sequences:

>Translated_600_residues
MEQRAPEPRAHETGRQEMTGAEMVVQALKDNGVEHIFGYPGGAVLPIYDELFQQDQIQHILVRHEQGAGHAAEGYARSTG
KAGVMLVTSGPGATNAVTPLQDALMDSIPLVCITGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVRDVSDLAAILHEAF
HVATTGRPGPVVVDIPKDVQFARGIYTPPQTAPRTSYQPTLDGDQEKIRAAVALMASARRPIIYTGGGVINAGPEASHLL
RELVGLTGFPITSTLMGLGAYPASGENWLGMLGMHGTYEANMAMHDCDVMLCIGARFDDRITGRLDAFSPNSRKIHVDVD
PSSINKNVRVDIPIIGDAGRVLEDMVRLWRASANADKAALKPWWEQINRWRARQSLAYKTNSDVIMPQYAVERLYELTKG
RDVYVTTEVGQHQMWAAQHFGFDQPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVLMTMQEMSSAVQHDA
PIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALPDFVKLAEAYGCHGIRCEKPGELDDAIREMIDVKKPVIFDCRVAN
LANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAEGRALV
>Mature_600_residues
MEQRAPEPRAHETGRQEMTGAEMVVQALKDNGVEHIFGYPGGAVLPIYDELFQQDQIQHILVRHEQGAGHAAEGYARSTG
KAGVMLVTSGPGATNAVTPLQDALMDSIPLVCITGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVRDVSDLAAILHEAF
HVATTGRPGPVVVDIPKDVQFARGIYTPPQTAPRTSYQPTLDGDQEKIRAAVALMASARRPIIYTGGGVINAGPEASHLL
RELVGLTGFPITSTLMGLGAYPASGENWLGMLGMHGTYEANMAMHDCDVMLCIGARFDDRITGRLDAFSPNSRKIHVDVD
PSSINKNVRVDIPIIGDAGRVLEDMVRLWRASANADKAALKPWWEQINRWRARQSLAYKTNSDVIMPQYAVERLYELTKG
RDVYVTTEVGQHQMWAAQHFGFDQPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVLMTMQEMSSAVQHDA
PIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALPDFVKLAEAYGCHGIRCEKPGELDDAIREMIDVKKPVIFDCRVAN
LANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAEGRALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=24.2160278745645, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI21361361, Length=577, Percent_Identity=24.263431542461, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=50.8802816901408, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=39.8936170212766, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=34.2756183745583, Blast_Score=315, Evalue=8e-87,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=27.9197080291971, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.7194244604317, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=546, Percent_Identity=26.5567765567766, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=546, Percent_Identity=26.5567765567766, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=526, Percent_Identity=25.8555133079848, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=45.1890034364261, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=25.7606490872211, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=27.3100616016427, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65515; Mature: 65515

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQRAPEPRAHETGRQEMTGAEMVVQALKDNGVEHIFGYPGGAVLPIYDELFQQDQIQHI
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHHHH
LVRHEQGAGHAAEGYARSTGKAGVMLVTSGPGATNAVTPLQDALMDSIPLVCITGQVPTP
HHCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCC
LIGSDAFQECDTVGITRPCTKHNWLVRDVSDLAAILHEAFHVATTGRPGPVVVDIPKDVQ
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCHH
FARGIYTPPQTAPRTSYQPTLDGDQEKIRAAVALMASARRPIIYTGGGVINAGPEASHLL
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHH
RELVGLTGFPITSTLMGLGAYPASGENWLGMLGMHGTYEANMAMHDCDVMLCIGARFDDR
HHHHCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCEECCCEEECCCEEEEECCCCCCC
ITGRLDAFSPNSRKIHVDVDPSSINKNVRVDIPIIGDAGRVLEDMVRLWRASANADKAAL
CCCEEECCCCCCCEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHH
KPWWEQINRWRARQSLAYKTNSDVIMPQYAVERLYELTKGRDVYVTTEVGQHQMWAAQHF
HHHHHHHHHHHHHHHHEEECCCCEECHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHC
GFDQPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVLMTMQEMSSAVQHDA
CCCCCCCEEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCC
PIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALPDFVKLAEAYGCHGIRCEKPGELDD
CEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHH
AIREMIDVKKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAEGRALV
HHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEQRAPEPRAHETGRQEMTGAEMVVQALKDNGVEHIFGYPGGAVLPIYDELFQQDQIQHI
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHHHH
LVRHEQGAGHAAEGYARSTGKAGVMLVTSGPGATNAVTPLQDALMDSIPLVCITGQVPTP
HHCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCC
LIGSDAFQECDTVGITRPCTKHNWLVRDVSDLAAILHEAFHVATTGRPGPVVVDIPKDVQ
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCHH
FARGIYTPPQTAPRTSYQPTLDGDQEKIRAAVALMASARRPIIYTGGGVINAGPEASHLL
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHH
RELVGLTGFPITSTLMGLGAYPASGENWLGMLGMHGTYEANMAMHDCDVMLCIGARFDDR
HHHHCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCEECCCEEECCCEEEEECCCCCCC
ITGRLDAFSPNSRKIHVDVDPSSINKNVRVDIPIIGDAGRVLEDMVRLWRASANADKAAL
CCCEEECCCCCCCEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHH
KPWWEQINRWRARQSLAYKTNSDVIMPQYAVERLYELTKGRDVYVTTEVGQHQMWAAQHF
HHHHHHHHHHHHHHHHEEECCCCEECHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHC
GFDQPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVLMTMQEMSSAVQHDA
CCCCCCCEEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCC
PIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALPDFVKLAEAYGCHGIRCEKPGELDD
CEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHH
AIREMIDVKKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAEGRALV
HHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]