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The map label for this gene is nasD [H]

Identifier: 110633804

GI number: 110633804

Start: 1544554

End: 1546998

Strand: Direct

Name: nasD [H]

Synonym: Meso_1451

Alternate gene names: 110633804

Gene position: 1544554-1546998 (Clockwise)

Preceding gene: 110633803

Following gene: 110633805

Centisome position: 35.0

GC content: 62.9

Gene sequence:

>2445_bases
ATGACTCAGAAACTCGTGGTGGTCGGCGCCGGCATGGCCTCCGGCCGCATGCTCGAGCACCTCTTCGAGGCAGCCCCCGG
TGCATTCGATGTGACGCTCTTCGGAGCCGAACCGCGCGGAAATTACAACCGCATCATGCTCTCGCCCGTGCTGGCCGGTG
AGAAGACCTACGACCAGATCGTCACCCATGACGCGGAATGGTACGCGGCCAATGATGTCCACTGCCGCTTCGGCGAGACG
GTGACGAAGATCGATCGCAAGGCGAAGGTGGTCTTCTCCAGGAGCGGCGAGACGCCTTACGATAAGCTCGTCGTCGCCAC
CGGCTCCGCGCCCTTCATCATCCCGATCGCCGGCAAGGACCTGCCGGGCGTGCGTGCCTTCCGCGACCTCGATGATGTCG
ACGCCATGGTGGCCGCCGCCGGCAGGCCCGGCGCCAAGGCGGTGGTGATCGGCGGCGGCCTTTTGGGACTGGAGGCTGCG
GCCGCGCTCAGGCTGCGCGGCATGGACGTGGTGGTGCTTCACCTCATGGGCCATCTCATGGAGCGCCAGCTCGATCCCGC
CGCCGGCTATCTGCTCCAGAAGGAACTGGAGGGCAGGGGCATAAAAATTCACTGCAAGGCGCAGACCAAGGCCATTCTTG
GCGATGCCCAGGTGGAAGCCGTGCTTCTGGAGGACGGCACGATCTATCCGGCAGATATCGTGGTGATGGCCGTCGGCATC
AGGCCTGAGGTGCGCATCGCGGTCGATGCGGGCCTTTATGTCGAGCGCGGCATTGTCGTGAACGACCAGATGGTAACCTC
CGATCCTGACATTCTCGCCATCGGAGAATGCGTGGAGCACGAGAGCATCTGCTACGGGCTCGTCGCTCCCCTCTATGAGA
TGGCCAAGGTGGCGGCCAAGACGCTGGTCAAGCAGGATGCGGCCTTCCGGCCGGTGGAAACTGCGACACAGCTTAAGGTT
ACCGGCGTAACCCTTTATTCCGCTGGCGATTTCGCCGATGCCGAGGATCGCGAGGAAATCGTGCTGCGCGATGCCAGTGC
CGGCGTCTACAAGCGCCTGGTACTGAAGGAAAACCGTATCCTCGGCGCGGTTCTCTACGGCGAGACGTCGGATGGGGCCT
GGTTCTTCGACATGCTGAGAAAGGGCACCGATGTTTCGGAGATGCGCGAAACCCTGATTTTCGGACAGGCCTACCAGGGA
GGGGGGCAACTGGACCCTATGGCGGCCGTTGCAGCCTTGCCGGATGATGCGGAAATCTGCGGCTGCAACGGCGTATGCAA
GAGCAAGATCACCGGCACCATTCAAGAAAAAGGCCTGACGACGCTTGACGCCGTGCGGGCCCACACCAAGGCCTCCGCCT
CCTGCGGAACCTGCACAGGGCTTGTCGAGCAGCTCATGAAGCTCACGCTCGGCGACGCTTTCAATCCGGCCGCCGTCACG
CCCATGTGCGGTTGCACCGACCTTGGCCACGACGACGTGCGCCGGCTGATCAAGGCATCGAGGCTCAAGACCATTCCAGC
CGTCATGCAGGAGCTGGAATGGAAGACCTCCTGCGGCTGCGCCAAGTGCCGGCCGGCGCTCAACTACTATCTCGTCTGCG
ATTGGCCGGACGAATATGCCGACGACTACCAGTCGCGCTTCATCAATGAGCGTGTTCACGCGAATATCCAGAAGGACGGC
ACCTATTCGGTGGTCCCGCGCATGTGGGGCGGCGTGACGAGTGCTGCGGAGCTGCGCGCCATCGCGGATGTGGTGGACAA
GTTCAACATCCCCACGGTGAAGGTCACCGGCGGCCAGCGCATCGACATGCTGGGCATCAAGAAGGAGGACTTGCCCGAGG
TTTGGGCCGATCTCGGCAAGGCTGGCTTCGTCTCCGGCCACGCCTATGCCAAGGGCTTGCGCACGGTGAAGACCTGCGTC
GGGTCGGACTGGTGCCGCTTCGGCACGCAGGACTCGACCGGCCTTGGCATCCGAATCGAGAAATTCATGTGGGGCTCGTG
GACGCCCGCGAAAGTGAAGATGGCCGTCTCTGGCTGCCCGCGCAACTGCGCGGAAGCCACTTGCAAGGATGTCGGCGTCA
TCTGCGTCGATTCCGGCTTCGAGATCCACTTCGCGGGCGCGGCGGGCCTCGATATCAAGGGCACGGAAAAGCTCGGCCTG
GTCAGATCGGAGGACGAGGCGCTGGAGGTGATCGTCGCGCTTACGCAGATGTACCGCGAGCAGGCGCGATATCTGGAGCG
CATCTACAAATGGGCCAAGCGCATCGGCTATGACGAGATCCGCCGCCAAATTCTGGACGATCCCGAAAAGCGCAAAGCCT
ATTACGACCGCTTTGTCTTCAGCCAGAAATTCGCCCAGGTGGACCCCTGGTCCGAGCGCGCCTCGGGCAAGGACAAGCAC
GAGTTCAAGCCGATGGCCACGCTGACCCTGGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
TCGTCTGGATGCACATCGCCATCCGCCAGATGGAGCGCCGCCAAGCGGGCCTGCAGCCCGCCTGAAAGCGCCACGCGAAA
CAAAGAGGAAACTTGTTCAA

Downstream 100 bases:

>100_bases
GCACCATGAACTGGTTCGCTATCGGTAAGCTCGAGGATATCCCCCTCCGCGGCGCGCGCTGCGTGGAGACCGGCAACACC
AAAATTGCCGTATTCCGTAC

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MTQKLVVVGAGMASGRMLEHLFEAAPGAFDVTLFGAEPRGNYNRIMLSPVLAGEKTYDQIVTHDAEWYAANDVHCRFGET
VTKIDRKAKVVFSRSGETPYDKLVVATGSAPFIIPIAGKDLPGVRAFRDLDDVDAMVAAAGRPGAKAVVIGGGLLGLEAA
AALRLRGMDVVVLHLMGHLMERQLDPAAGYLLQKELEGRGIKIHCKAQTKAILGDAQVEAVLLEDGTIYPADIVVMAVGI
RPEVRIAVDAGLYVERGIVVNDQMVTSDPDILAIGECVEHESICYGLVAPLYEMAKVAAKTLVKQDAAFRPVETATQLKV
TGVTLYSAGDFADAEDREEIVLRDASAGVYKRLVLKENRILGAVLYGETSDGAWFFDMLRKGTDVSEMRETLIFGQAYQG
GGQLDPMAAVAALPDDAEICGCNGVCKSKITGTIQEKGLTTLDAVRAHTKASASCGTCTGLVEQLMKLTLGDAFNPAAVT
PMCGCTDLGHDDVRRLIKASRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKDG
TYSVVPRMWGGVTSAAELRAIADVVDKFNIPTVKVTGGQRIDMLGIKKEDLPEVWADLGKAGFVSGHAYAKGLRTVKTCV
GSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGTEKLGL
VRSEDEALEVIVALTQMYREQARYLERIYKWAKRIGYDEIRRQILDDPEKRKAYYDRFVFSQKFAQVDPWSERASGKDKH
EFKPMATLTLEAAE

Sequences:

>Translated_814_residues
MTQKLVVVGAGMASGRMLEHLFEAAPGAFDVTLFGAEPRGNYNRIMLSPVLAGEKTYDQIVTHDAEWYAANDVHCRFGET
VTKIDRKAKVVFSRSGETPYDKLVVATGSAPFIIPIAGKDLPGVRAFRDLDDVDAMVAAAGRPGAKAVVIGGGLLGLEAA
AALRLRGMDVVVLHLMGHLMERQLDPAAGYLLQKELEGRGIKIHCKAQTKAILGDAQVEAVLLEDGTIYPADIVVMAVGI
RPEVRIAVDAGLYVERGIVVNDQMVTSDPDILAIGECVEHESICYGLVAPLYEMAKVAAKTLVKQDAAFRPVETATQLKV
TGVTLYSAGDFADAEDREEIVLRDASAGVYKRLVLKENRILGAVLYGETSDGAWFFDMLRKGTDVSEMRETLIFGQAYQG
GGQLDPMAAVAALPDDAEICGCNGVCKSKITGTIQEKGLTTLDAVRAHTKASASCGTCTGLVEQLMKLTLGDAFNPAAVT
PMCGCTDLGHDDVRRLIKASRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKDG
TYSVVPRMWGGVTSAAELRAIADVVDKFNIPTVKVTGGQRIDMLGIKKEDLPEVWADLGKAGFVSGHAYAKGLRTVKTCV
GSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGTEKLGL
VRSEDEALEVIVALTQMYREQARYLERIYKWAKRIGYDEIRRQILDDPEKRKAYYDRFVFSQKFAQVDPWSERASGKDKH
EFKPMATLTLEAAE
>Mature_813_residues
TQKLVVVGAGMASGRMLEHLFEAAPGAFDVTLFGAEPRGNYNRIMLSPVLAGEKTYDQIVTHDAEWYAANDVHCRFGETV
TKIDRKAKVVFSRSGETPYDKLVVATGSAPFIIPIAGKDLPGVRAFRDLDDVDAMVAAAGRPGAKAVVIGGGLLGLEAAA
ALRLRGMDVVVLHLMGHLMERQLDPAAGYLLQKELEGRGIKIHCKAQTKAILGDAQVEAVLLEDGTIYPADIVVMAVGIR
PEVRIAVDAGLYVERGIVVNDQMVTSDPDILAIGECVEHESICYGLVAPLYEMAKVAAKTLVKQDAAFRPVETATQLKVT
GVTLYSAGDFADAEDREEIVLRDASAGVYKRLVLKENRILGAVLYGETSDGAWFFDMLRKGTDVSEMRETLIFGQAYQGG
GQLDPMAAVAALPDDAEICGCNGVCKSKITGTIQEKGLTTLDAVRAHTKASASCGTCTGLVEQLMKLTLGDAFNPAAVTP
MCGCTDLGHDDVRRLIKASRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKDGT
YSVVPRMWGGVTSAAELRAIADVVDKFNIPTVKVTGGQRIDMLGIKKEDLPEVWADLGKAGFVSGHAYAKGLRTVKTCVG
SDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGTEKLGLV
RSEDEALEVIVALTQMYREQARYLERIYKWAKRIGYDEIRRQILDDPEKRKAYYDRFVFSQKFAQVDPWSERASGKDKHE
FKPMATLTLEAAE

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=282, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI226437568, Length=282, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI21389617, Length=282, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=9e-18,
Organism=Escherichia coli, GI1789765, Length=807, Percent_Identity=35.6877323420074, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1789065, Length=343, Percent_Identity=27.6967930029155, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=29.136690647482, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=406, Percent_Identity=23.3990147783251, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281359715, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281359713, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24639250, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI18543267, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24639252, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24639257, Length=240, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88651; Mature: 88520

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLVVVGAGMASGRMLEHLFEAAPGAFDVTLFGAEPRGNYNRIMLSPVLAGEKTYDQI
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHH
VTHDAEWYAANDVHCRFGETVTKIDRKAKVVFSRSGETPYDKLVVATGSAPFIIPIAGKD
HCCCCCEEECCCCEEECCCHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
LPGVRAFRDLDDVDAMVAAAGRPGAKAVVIGGGLLGLEAAAALRLRGMDVVVLHLMGHLM
CCCHHHHHCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ERQLDPAAGYLLQKELEGRGIKIHCKAQTKAILGDAQVEAVLLEDGTIYPADIVVMAVGI
HHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEEEEECCCCCCCEEEEEEECC
RPEVRIAVDAGLYVERGIVVNDQMVTSDPDILAIGECVEHESICYGLVAPLYEMAKVAAK
CCCEEEEEECCEEEECCEEECCCEECCCCCEEEECHHHCHHHHHHHHHHHHHHHHHHHHH
TLVKQDAAFRPVETATQLKVTGVTLYSAGDFADAEDREEIVLRDASAGVYKRLVLKENRI
HHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCE
LGAVLYGETSDGAWFFDMLRKGTDVSEMRETLIFGQAYQGGGQLDPMAAVAALPDDAEIC
EEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEE
GCNGVCKSKITGTIQEKGLTTLDAVRAHTKASASCGTCTGLVEQLMKLTLGDAFNPAAVT
CCCCCHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
PMCGCTDLGHDDVRRLIKASRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHHH
DDYQSRFINERVHANIQKDGTYSVVPRMWGGVTSAAELRAIADVVDKFNIPTVKVTGGQR
HHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCE
IDMLGIKKEDLPEVWADLGKAGFVSGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRIE
EEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEE
KFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGTEKLGL
EEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCCC
VRSEDEALEVIVALTQMYREQARYLERIYKWAKRIGYDEIRRQILDDPEKRKAYYDRFVF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
SQKFAQVDPWSERASGKDKHEFKPMATLTLEAAE
HHHHHCCCCCHHHCCCCCCHHCCCEEEEEEEECC
>Mature Secondary Structure 
TQKLVVVGAGMASGRMLEHLFEAAPGAFDVTLFGAEPRGNYNRIMLSPVLAGEKTYDQI
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHH
VTHDAEWYAANDVHCRFGETVTKIDRKAKVVFSRSGETPYDKLVVATGSAPFIIPIAGKD
HCCCCCEEECCCCEEECCCHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
LPGVRAFRDLDDVDAMVAAAGRPGAKAVVIGGGLLGLEAAAALRLRGMDVVVLHLMGHLM
CCCHHHHHCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ERQLDPAAGYLLQKELEGRGIKIHCKAQTKAILGDAQVEAVLLEDGTIYPADIVVMAVGI
HHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEEEEECCCCCCCEEEEEEECC
RPEVRIAVDAGLYVERGIVVNDQMVTSDPDILAIGECVEHESICYGLVAPLYEMAKVAAK
CCCEEEEEECCEEEECCEEECCCEECCCCCEEEECHHHCHHHHHHHHHHHHHHHHHHHHH
TLVKQDAAFRPVETATQLKVTGVTLYSAGDFADAEDREEIVLRDASAGVYKRLVLKENRI
HHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCE
LGAVLYGETSDGAWFFDMLRKGTDVSEMRETLIFGQAYQGGGQLDPMAAVAALPDDAEIC
EEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEE
GCNGVCKSKITGTIQEKGLTTLDAVRAHTKASASCGTCTGLVEQLMKLTLGDAFNPAAVT
CCCCCHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
PMCGCTDLGHDDVRRLIKASRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHHH
DDYQSRFINERVHANIQKDGTYSVVPRMWGGVTSAAELRAIADVVDKFNIPTVKVTGGQR
HHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCE
IDMLGIKKEDLPEVWADLGKAGFVSGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRIE
EEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEE
KFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGFEIHFAGAAGLDIKGTEKLGL
EEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCCC
VRSEDEALEVIVALTQMYREQARYLERIYKWAKRIGYDEIRRQILDDPEKRKAYYDRFVF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
SQKFAQVDPWSERASGKDKHEFKPMATLTLEAAE
HHHHHCCCCCHHHCCCCCCHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]