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The map label for this gene is tgt [H]

Identifier: 110633759

GI number: 110633759

Start: 1502171

End: 1503301

Strand: Direct

Name: tgt [H]

Synonym: Meso_1406

Alternate gene names: 110633759

Gene position: 1502171-1503301 (Clockwise)

Preceding gene: 110633758

Following gene: 110633762

Centisome position: 34.04

GC content: 65.25

Gene sequence:

>1131_bases
GTGAGCGACACCTTTACCTTCCGCGTGCTCGCCACCGACGGCAAGGCGCGGCGCGGCGAAATCTCCATGCCGCGCGGCAA
CATCCGTACGCCGGCCTTCATGCCCGTAGGCACCGGCGGCACGGTGAAGGCGATGTATATGGACCAGGTGCGGGCGCTGG
GCTCCGACATTATCCTGGGCAACACCTATCATCTCATGCTGCGACCGGGTGCCGAGCGCGTGGCCCGGCTCGGCGGGCTG
CACGAATTTGCCCGCTGGCCTTATCCAATCCTAACCGATTCCGGCGGTTTCCAGGTGATGTCGCTTTCGGCCCTGCGCAA
GGTGAGGGAGGAGGGCGTCACCTTCCGCTCCCATGTGGATGGCGAGGTGCACGAGATGTCGCCCGAGCGGTCCATCGAGA
TCCAGGGTCTGCTCGATTCCGACATCCAGATGCAGCTCGACGAGTGCATCGCGCTGCCGTCGCCGGAAAAGGAAATCGAG
CGGGCGATGGAGCTTTCGCTGCGCTGGGCCGAACGCTGCAAAATCGCTTTCGGCGATCAACCGGGCAAGGCCATGTTCGG
TATCGTTCAGGGCGGAGACAACCGGCGGCTCCGGCTACGCTCTGCCGAAGCGCTCGCTGCGATGGGGCTGAAAGGCTATG
CCGTGGGCGGGCTCGCGGTGGGCGAGCCGCAGGAGGTGATGCTCGACATTCTCGATGCAACCTGCCCTGCGCTGCCGGCT
AACAAGCCGCGTTACCTGATGGGCGTTGGCACGCCCGACGACATCTTGAAGTCCGTTGCGCGCGGCATCGACATGTTCGA
CTGCGTGATGCCGACCCGGGCGGGCCGCCATGGGCTTGCGTTCACCCGGCGCGGCAAAATCAACCTGAAGAACGCCCGCC
ACGCGGAGGACACGCGTCCGTTGGACGAGGAGAGCGATTGCCCGGCCGCGCGTGATTATTCCCGCGCCTATCTGCATCAC
CTGGTGAAATCCGGCGAGGCGCTGGGCGGCATGCTGCTCACCTGGAACAATCTCGCCTATTACCAGGGCCTGATGGCCGA
CATCCGAGCCGCCATTGAAGCCGGCCGGCTGGGCGAACGCACGGCGGAAATCCAGGAAGAGTGGGTGCGCGGCGACTTGC
CGGCGCTTTAG

Upstream 100 bases:

>100_bases
GCGGGCTTGAGACGATGAAATCCGCCTACAGCCATGCCATCGACCAGGGCTACCGGTTTTACTCCTACGGCGATTCCAGC
CTGCTTTTCAGGGAAGACAC

Downstream 100 bases:

>100_bases
GGCGTCATACCGCTGCGTCTGGCGCAGGCCGCGCAAGGCGGGGACGGAGATCAAGCGCCTAAACGATTTGTGCGCCTTGT
GCGCGGGCCCGGCCACGCCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSDTFTFRVLATDGKARRGEISMPRGNIRTPAFMPVGTGGTVKAMYMDQVRALGSDIILGNTYHLMLRPGAERVARLGGL
HEFARWPYPILTDSGGFQVMSLSALRKVREEGVTFRSHVDGEVHEMSPERSIEIQGLLDSDIQMQLDECIALPSPEKEIE
RAMELSLRWAERCKIAFGDQPGKAMFGIVQGGDNRRLRLRSAEALAAMGLKGYAVGGLAVGEPQEVMLDILDATCPALPA
NKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRRGKINLKNARHAEDTRPLDEESDCPAARDYSRAYLHH
LVKSGEALGGMLLTWNNLAYYQGLMADIRAAIEAGRLGERTAEIQEEWVRGDLPAL

Sequences:

>Translated_376_residues
MSDTFTFRVLATDGKARRGEISMPRGNIRTPAFMPVGTGGTVKAMYMDQVRALGSDIILGNTYHLMLRPGAERVARLGGL
HEFARWPYPILTDSGGFQVMSLSALRKVREEGVTFRSHVDGEVHEMSPERSIEIQGLLDSDIQMQLDECIALPSPEKEIE
RAMELSLRWAERCKIAFGDQPGKAMFGIVQGGDNRRLRLRSAEALAAMGLKGYAVGGLAVGEPQEVMLDILDATCPALPA
NKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRRGKINLKNARHAEDTRPLDEESDCPAARDYSRAYLHH
LVKSGEALGGMLLTWNNLAYYQGLMADIRAAIEAGRLGERTAEIQEEWVRGDLPAL
>Mature_375_residues
SDTFTFRVLATDGKARRGEISMPRGNIRTPAFMPVGTGGTVKAMYMDQVRALGSDIILGNTYHLMLRPGAERVARLGGLH
EFARWPYPILTDSGGFQVMSLSALRKVREEGVTFRSHVDGEVHEMSPERSIEIQGLLDSDIQMQLDECIALPSPEKEIER
AMELSLRWAERCKIAFGDQPGKAMFGIVQGGDNRRLRLRSAEALAAMGLKGYAVGGLAVGEPQEVMLDILDATCPALPAN
KPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRRGKINLKNARHAEDTRPLDEESDCPAARDYSRAYLHHL
VKSGEALGGMLLTWNNLAYYQGLMADIRAAIEAGRLGERTAEIQEEWVRGDLPAL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=343, Percent_Identity=43.731778425656, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI13375872, Length=288, Percent_Identity=26.0416666666667, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1786607, Length=352, Percent_Identity=53.6931818181818, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17544680, Length=352, Percent_Identity=44.3181818181818, Blast_Score=294, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920510, Length=383, Percent_Identity=42.2976501305483, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21355311, Length=261, Percent_Identity=24.5210727969349, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41397; Mature: 41266

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTFTFRVLATDGKARRGEISMPRGNIRTPAFMPVGTGGTVKAMYMDQVRALGSDIILG
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCEEEC
NTYHLMLRPGAERVARLGGLHEFARWPYPILTDSGGFQVMSLSALRKVREEGVTFRSHVD
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCC
GEVHEMSPERSIEIQGLLDSDIQMQLDECIALPSPEKEIERAMELSLRWAERCKIAFGDQ
CHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEECCC
PGKAMFGIVQGGDNRRLRLRSAEALAAMGLKGYAVGGLAVGEPQEVMLDILDATCPALPA
CCCEEEEEEECCCCCEEEEEHHHHHHHHCCCCEEECCEECCCHHHHHHHHHHHCCCCCCC
NKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRRGKINLKNARHAEDTRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
LDEESDCPAARDYSRAYLHHLVKSGEALGGMLLTWNNLAYYQGLMADIRAAIEAGRLGER
CCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHH
TAEIQEEWVRGDLPAL
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDTFTFRVLATDGKARRGEISMPRGNIRTPAFMPVGTGGTVKAMYMDQVRALGSDIILG
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCEEEC
NTYHLMLRPGAERVARLGGLHEFARWPYPILTDSGGFQVMSLSALRKVREEGVTFRSHVD
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCC
GEVHEMSPERSIEIQGLLDSDIQMQLDECIALPSPEKEIERAMELSLRWAERCKIAFGDQ
CHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEECCC
PGKAMFGIVQGGDNRRLRLRSAEALAAMGLKGYAVGGLAVGEPQEVMLDILDATCPALPA
CCCEEEEEEECCCCCEEEEEHHHHHHHHCCCCEEECCEECCCHHHHHHHHHHHCCCCCCC
NKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRRGKINLKNARHAEDTRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
LDEESDCPAARDYSRAYLHHLVKSGEALGGMLLTWNNLAYYQGLMADIRAAIEAGRLGER
CCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHH
TAEIQEEWVRGDLPAL
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]