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The map label for this gene is recE [H]

Identifier: 110633541

GI number: 110633541

Start: 1295208

End: 1296194

Strand: Reverse

Name: recE [H]

Synonym: Meso_1188

Alternate gene names: 110633541

Gene position: 1296194-1295208 (Counterclockwise)

Preceding gene: 110633542

Following gene: 110633540

Centisome position: 29.38

GC content: 60.49

Gene sequence:

>987_bases
ATGACGATGCAAAGCCTGGGCGATGTCGTCGGAAACCTTGTCGACGGTATCGGAAACGAGCGGTACTGGCGTGGCGGCAA
GATCGCGGAGCCGGGCGTCTATGCCGGCGTCGACATAGAGCGTTATCACCATGACCCCGACCTGTTCGACGGGTTCTCAA
TTTCGAGCTCTGGCCTGCGCGCCCTGTTGCGCCGGCCGGCCGAATATTGGTGGTCGAGCCCATACAATCCAAGCAGCCAG
GAGCCCGAGAGCAAGCCCGCGCTCGATTTCGGCAAGGCCGCTCACATGCTTCTCTTGGGAGAGGATGGCTTTGCCGGACA
GTACTCCCTCCGCCCTGCGACCTACACCGACAGCAAGGGCGATGAGAAGCCTTGGAACGGCAACGCCACGTCGTGCAAGG
ACTGGCTTGCCCAGCAGGCGAAAGCCGGCCGGACGGTCATCACCGAAACCGATATTGCCCATATCCGGCACATGGCCGAC
GCGCTTTCGCGCAAAGAGGCAATCCGGCTTGGTATCCTCAATGGCAAGATTGAGCGATCCATCTTTCACAAGGACCGGGA
TATCTGGCTGAAGAGCCGCCCCGATGTAATCCCGATATCGTCTGGTGACTTCGTGGATCTGAAGACCGCGGCATCGGTCG
ATGACGAAACCCTGTCGCGGGCCATCTTCGCCCACGGCTACCACGTCCAAGCCGGTCTCCTGCGCGTGATCGTGCGGGAG
ATTCTTGGGCCCGACGCCTTCACCTCATTCACCTTCGTCTTTGTCGAGAAGGCCCCGCCCTATGACGTGCGGGTGATGCA
GCTCAAGGACGCTGACATCGATATTGGTGAGCGCCAGGCGCGGCAAGGTCTGAAGCTCCTGCGTGAGTGCCTGCGGCGCC
AGGAATGGCCCGGATATGACGGCTTCGATCAGCACATCTCGTACATCGAAATGCCTTCCTGGGCGAAGACCCGGATTGAC
AACGAACTCAATGCGGAGGCCGCATAA

Upstream 100 bases:

>100_bases
GCCGGTTTGAGGCACTGAGACCAGCCATCCGCGATCAATTTGAAGCTGAAGCCAAGGCGGCAATCCGCGTGGTGGAAGCC
TTTCAGCAGGAGCAATTCGC

Downstream 100 bases:

>100_bases
CCATGAACCAGATCGCCACTCGTGAAGCGTCCATAACTCAGGTCGGCATTGCCACCGGAGCCGGTGGATCGAGCATAGCA
CCGCAGAACCTCGGCGAGGT

Product: hypothetical protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTMQSLGDVVGNLVDGIGNERYWRGGKIAEPGVYAGVDIERYHHDPDLFDGFSISSSGLRALLRRPAEYWWSSPYNPSSQ
EPESKPALDFGKAAHMLLLGEDGFAGQYSLRPATYTDSKGDEKPWNGNATSCKDWLAQQAKAGRTVITETDIAHIRHMAD
ALSRKEAIRLGILNGKIERSIFHKDRDIWLKSRPDVIPISSGDFVDLKTAASVDDETLSRAIFAHGYHVQAGLLRVIVRE
ILGPDAFTSFTFVFVEKAPPYDVRVMQLKDADIDIGERQARQGLKLLRECLRRQEWPGYDGFDQHISYIEMPSWAKTRID
NELNAEAA

Sequences:

>Translated_328_residues
MTMQSLGDVVGNLVDGIGNERYWRGGKIAEPGVYAGVDIERYHHDPDLFDGFSISSSGLRALLRRPAEYWWSSPYNPSSQ
EPESKPALDFGKAAHMLLLGEDGFAGQYSLRPATYTDSKGDEKPWNGNATSCKDWLAQQAKAGRTVITETDIAHIRHMAD
ALSRKEAIRLGILNGKIERSIFHKDRDIWLKSRPDVIPISSGDFVDLKTAASVDDETLSRAIFAHGYHVQAGLLRVIVRE
ILGPDAFTSFTFVFVEKAPPYDVRVMQLKDADIDIGERQARQGLKLLRECLRRQEWPGYDGFDQHISYIEMPSWAKTRID
NELNAEAA
>Mature_327_residues
TMQSLGDVVGNLVDGIGNERYWRGGKIAEPGVYAGVDIERYHHDPDLFDGFSISSSGLRALLRRPAEYWWSSPYNPSSQE
PESKPALDFGKAAHMLLLGEDGFAGQYSLRPATYTDSKGDEKPWNGNATSCKDWLAQQAKAGRTVITETDIAHIRHMADA
LSRKEAIRLGILNGKIERSIFHKDRDIWLKSRPDVIPISSGDFVDLKTAASVDDETLSRAIFAHGYHVQAGLLRVIVREI
LGPDAFTSFTFVFVEKAPPYDVRVMQLKDADIDIGERQARQGLKLLRECLRRQEWPGYDGFDQHISYIEMPSWAKTRIDN
ELNAEAA

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=300, Percent_Identity=26.3333333333333, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 36797; Mature: 36666

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMQSLGDVVGNLVDGIGNERYWRGGKIAEPGVYAGVDIERYHHDPDLFDGFSISSSGLR
CCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCCCHHHHH
ALLRRPAEYWWSSPYNPSSQEPESKPALDFGKAAHMLLLGEDGFAGQYSLRPATYTDSKG
HHHHCCHHHHCCCCCCCCCCCCCCCCCCHHCCCEEEEEECCCCCCCCEECCCCCCCCCCC
DEKPWNGNATSCKDWLAQQAKAGRTVITETDIAHIRHMADALSRKEAIRLGILNGKIERS
CCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHH
IFHKDRDIWLKSRPDVIPISSGDFVDLKTAASVDDETLSRAIFAHGYHVQAGLLRVIVRE
HHHCCCCEEECCCCCEEECCCCCEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ILGPDAFTSFTFVFVEKAPPYDVRVMQLKDADIDIGERQARQGLKLLRECLRRQEWPGYD
HHCCCHHHCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GFDQHISYIEMPSWAKTRIDNELNAEAA
CHHHCCHHHCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TMQSLGDVVGNLVDGIGNERYWRGGKIAEPGVYAGVDIERYHHDPDLFDGFSISSSGLR
CHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCCCHHHHH
ALLRRPAEYWWSSPYNPSSQEPESKPALDFGKAAHMLLLGEDGFAGQYSLRPATYTDSKG
HHHHCCHHHHCCCCCCCCCCCCCCCCCCHHCCCEEEEEECCCCCCCCEECCCCCCCCCCC
DEKPWNGNATSCKDWLAQQAKAGRTVITETDIAHIRHMADALSRKEAIRLGILNGKIERS
CCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHH
IFHKDRDIWLKSRPDVIPISSGDFVDLKTAASVDDETLSRAIFAHGYHVQAGLLRVIVRE
HHHCCCCEEECCCCCEEECCCCCEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ILGPDAFTSFTFVFVEKAPPYDVRVMQLKDADIDIGERQARQGLKLLRECLRRQEWPGYD
HHCCCHHHCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GFDQHISYIEMPSWAKTRIDNELNAEAA
CHHHCCHHHCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]