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The map label for this gene is aspS

Identifier: 110633475

GI number: 110633475

Start: 1227390

End: 1229174

Strand: Direct

Name: aspS

Synonym: Meso_1122

Alternate gene names: 110633475

Gene position: 1227390-1229174 (Clockwise)

Preceding gene: 110633473

Following gene: 110633477

Centisome position: 27.82

GC content: 62.41

Gene sequence:

>1785_bases
ATGCACCGTTACCGCAGCCACACCTGCGAAGAACTGAGGCCATCGGATGTGGGCGGGACCGTCCGGCTATCCGGCTGGGT
CCACCGCGTGCGCGATCATGGGGGTCTGCTCTTCATCGACCTGCGTGATCATTACGGCATCACGCAGATTGTCGCCGACC
CGGATTCGCCCTCATTCAGCACGGCCGAGAAGCTGCGTAGCGAGTGGGTGATCCGCGTTGACGGTGAGGTCAAGGCACGC
ACGCCGGAAACGGTCAACCCGAATCTGGCCACTGGCGGTGTGGAGGTTTTCGCCCGCGAGATCGAGGTGCTGAGTCAGGC
CAAGGAGCTCCCGCTGCCGGTCTTCGGCGAGCCGGACTACCCGGAGGACATCCGCCTCAAATACCGCTTTCTGGATCTCC
GCCGCGAGACGCTGCACCGGAACATCCTCGCCCGCACGAAGATCATCGCGGAAATGCGCAAGCGGATGGCGGAATCCGGC
TTCACCGAATTCTCGACGCCCATCCTGACCGCCTCATCGCCTGAGGGAGCCCGCGACTTTCTGGTTCCTTCGCGGCTTCA
TCAGGGCAAGTTCTACGCGCTGCCGCAGGCGCCGCAGATCTATAAGCAGCTCATCATGGTCTCCGGTTTCGATCGCTATT
TCCAGATCGCGCCCTGCTTCCGCGATGAGGATCCGCGCGCCGATCGCCTTCCGGGCGAGTTTTACCAGCTCGACCTGGAG
ATGAGCTTCGTCACGCAGGAGGATGTGTTCTCCACGATGGAGCCCGTGATGCGCGGGATCTTCGAGGCGTTCGCCGAGGG
CAAGCCGGTCACGCAGAAACTCCCGCGCATTCCCTATGACGAGGCGATGCGCAAATATGGCTCCGACAAGCCGGACCTGC
GCAACCCGATCGTGATGGAGGAGGTTTCGGAGCATTTCCGCGGTTCCGGCTTCAAGGTTTTTGCCAATATCCTGGCAAAT
GACCCGAAAGCGGAAGTCTGGGCCATTCCCGCAAAGACCGGCGGAAGCCGTGCATTCTGCGACCGGATGAACTCCTGGGC
GCAAGGGGAGGGGCAACCGGGCCTCGGCTACATCTTCTGGCGCAAGGAGGGCGATACGCTCGAAGGCGCTGGTCCGATCG
CCAAGAATATCGGCCCGGAGCGCACGGACGCGATCCGCACCCAGCTTGGTCTCGGTGATGGGGATGCCTGTTTCTTCGTC
GCGGGCGATCCCAAGAAATTCGTGGCCTTCGCGGGCGCCGCGCGTACGCGCGCCGGCGAAGAACTGAACCTTGTCGACCG
TGATCGTTTCGAACTCGCCTGGATTGTCGACTTCCCGTTTTACGAGTGGAACGAGGACGAGAAGCGCATCGAGTTCGGCC
ACAACCCCTTTTCCATGCCGCAGGGCGGCATGGAGGCGTTGGAAAACCAGGAGCCATTGTCGATCAAAGCCTTTCAGTAC
GACTTGGTCTGCAACGGCTTCGAAATTGCGTCTGGCGGCATCCGCAACCACATGCCCGAACTGATGGTGAAGGCTTTCGA
GGTTGCGGGCTTCGACCGGCAGATGGTGGAGGAGCGCTTCGGCGGCCTATACCGTGCTTTCCAGTATGGCGCGCCGCCCC
ATGGCGGCATGGCGGCGGGCATCGATCGCATCGTCATGCTGCTCAGGGGGGCCAAGAACCTGCGCGAGATCACGATGTTC
CCCATGAACCAGCAGGCGATGGACCTGCTGATGGGGGCGCCTTCGGAAGCCACTCCGCAGCAGCTCCGCGAGCTGCACAT
CCGGCTCGCGCCGAAGCAGGATTGA

Upstream 100 bases:

>100_bases
CGCCTTGACAAAAGTGGGCGCACATGCGCTTTTCGCGCGGTTTTTGGACGGGCAATCGGGCCCGCTTCCGGTCACCCTTT
CAGGATACGGAATCGAAATA

Downstream 100 bases:

>100_bases
GCGGACAGCCTGCGGTTTGAGAAATCCGCAGGCTCGTCCGAACGGGCGCTACTGGTTTGCCTTCACCGCGAGCCCGATCT
GCTTCACGAGCATTTCCGGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MHRYRSHTCEELRPSDVGGTVRLSGWVHRVRDHGGLLFIDLRDHYGITQIVADPDSPSFSTAEKLRSEWVIRVDGEVKAR
TPETVNPNLATGGVEVFAREIEVLSQAKELPLPVFGEPDYPEDIRLKYRFLDLRRETLHRNILARTKIIAEMRKRMAESG
FTEFSTPILTASSPEGARDFLVPSRLHQGKFYALPQAPQIYKQLIMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVFSTMEPVMRGIFEAFAEGKPVTQKLPRIPYDEAMRKYGSDKPDLRNPIVMEEVSEHFRGSGFKVFANILAN
DPKAEVWAIPAKTGGSRAFCDRMNSWAQGEGQPGLGYIFWRKEGDTLEGAGPIAKNIGPERTDAIRTQLGLGDGDACFFV
AGDPKKFVAFAGAARTRAGEELNLVDRDRFELAWIVDFPFYEWNEDEKRIEFGHNPFSMPQGGMEALENQEPLSIKAFQY
DLVCNGFEIASGGIRNHMPELMVKAFEVAGFDRQMVEERFGGLYRAFQYGAPPHGGMAAGIDRIVMLLRGAKNLREITMF
PMNQQAMDLLMGAPSEATPQQLRELHIRLAPKQD

Sequences:

>Translated_594_residues
MHRYRSHTCEELRPSDVGGTVRLSGWVHRVRDHGGLLFIDLRDHYGITQIVADPDSPSFSTAEKLRSEWVIRVDGEVKAR
TPETVNPNLATGGVEVFAREIEVLSQAKELPLPVFGEPDYPEDIRLKYRFLDLRRETLHRNILARTKIIAEMRKRMAESG
FTEFSTPILTASSPEGARDFLVPSRLHQGKFYALPQAPQIYKQLIMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVFSTMEPVMRGIFEAFAEGKPVTQKLPRIPYDEAMRKYGSDKPDLRNPIVMEEVSEHFRGSGFKVFANILAN
DPKAEVWAIPAKTGGSRAFCDRMNSWAQGEGQPGLGYIFWRKEGDTLEGAGPIAKNIGPERTDAIRTQLGLGDGDACFFV
AGDPKKFVAFAGAARTRAGEELNLVDRDRFELAWIVDFPFYEWNEDEKRIEFGHNPFSMPQGGMEALENQEPLSIKAFQY
DLVCNGFEIASGGIRNHMPELMVKAFEVAGFDRQMVEERFGGLYRAFQYGAPPHGGMAAGIDRIVMLLRGAKNLREITMF
PMNQQAMDLLMGAPSEATPQQLRELHIRLAPKQD
>Mature_594_residues
MHRYRSHTCEELRPSDVGGTVRLSGWVHRVRDHGGLLFIDLRDHYGITQIVADPDSPSFSTAEKLRSEWVIRVDGEVKAR
TPETVNPNLATGGVEVFAREIEVLSQAKELPLPVFGEPDYPEDIRLKYRFLDLRRETLHRNILARTKIIAEMRKRMAESG
FTEFSTPILTASSPEGARDFLVPSRLHQGKFYALPQAPQIYKQLIMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVFSTMEPVMRGIFEAFAEGKPVTQKLPRIPYDEAMRKYGSDKPDLRNPIVMEEVSEHFRGSGFKVFANILAN
DPKAEVWAIPAKTGGSRAFCDRMNSWAQGEGQPGLGYIFWRKEGDTLEGAGPIAKNIGPERTDAIRTQLGLGDGDACFFV
AGDPKKFVAFAGAARTRAGEELNLVDRDRFELAWIVDFPFYEWNEDEKRIEFGHNPFSMPQGGMEALENQEPLSIKAFQY
DLVCNGFEIASGGIRNHMPELMVKAFEVAGFDRQMVEERFGGLYRAFQYGAPPHGGMAAGIDRIVMLLRGAKNLREITMF
PMNQQAMDLLMGAPSEATPQQLRELHIRLAPKQD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=33.7748344370861, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI45439306, Length=286, Percent_Identity=27.972027972028, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=24.901185770751, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI5031815, Length=275, Percent_Identity=28, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194272210, Length=275, Percent_Identity=28, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4758762, Length=246, Percent_Identity=25.2032520325203, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=42.4242424242424, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1789256, Length=302, Percent_Identity=27.1523178807947, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790571, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=608, Percent_Identity=32.8947368421053, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17551876, Length=282, Percent_Identity=28.0141843971631, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=292, Percent_Identity=26.3698630136986, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=652, Percent_Identity=29.601226993865, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=28.7313432835821, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=255, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=32.6732673267327, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI17136276, Length=266, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640851, Length=265, Percent_Identity=27.1698113207547, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=265, Percent_Identity=27.1698113207547, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_MESSB (Q11JA7)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673683.1
- ProteinModelPortal:   Q11JA7
- SMR:   Q11JA7
- STRING:   Q11JA7
- GeneID:   4182530
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1122
- NMPDR:   fig|266779.1.peg.2464
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   MSP266779:MESO_1122-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67144; Mature: 67144

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRSHTCEELRPSDVGGTVRLSGWVHRVRDHGGLLFIDLRDHYGITQIVADPDSPSFS
CCCCHHCCHHHCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCHH
TAEKLRSEWVIRVDGEVKARTPETVNPNLATGGVEVFAREIEVLSQAKELPLPVFGEPDY
HHHHHHHCEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLKYRFLDLRRETLHRNILARTKIIAEMRKRMAESGFTEFSTPILTASSPEGARDF
CHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCHHC
LVPSRLHQGKFYALPQAPQIYKQLIMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCHHHHCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFSTMEPVMRGIFEAFAEGKPVTQKLPRIPYDEAMRKYGSDKPDLRNPIVME
EEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
EVSEHFRGSGFKVFANILANDPKAEVWAIPAKTGGSRAFCDRMNSWAQGEGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
RKEGDTLEGAGPIAKNIGPERTDAIRTQLGLGDGDACFFVAGDPKKFVAFAGAARTRAGE
ECCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHEECCHHHHCCCC
ELNLVDRDRFELAWIVDFPFYEWNEDEKRIEFGHNPFSMPQGGMEALENQEPLSIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCCEEEEEEE
DLVCNGFEIASGGIRNHMPELMVKAFEVAGFDRQMVEERFGGLYRAFQYGAPPHGGMAAG
EEEECCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRIVMLLRGAKNLREITMFPMNQQAMDLLMGAPSEATPQQLRELHIRLAPKQD
HHHHHHHHHCCCCHHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHRYRSHTCEELRPSDVGGTVRLSGWVHRVRDHGGLLFIDLRDHYGITQIVADPDSPSFS
CCCCHHCCHHHCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCHH
TAEKLRSEWVIRVDGEVKARTPETVNPNLATGGVEVFAREIEVLSQAKELPLPVFGEPDY
HHHHHHHCEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLKYRFLDLRRETLHRNILARTKIIAEMRKRMAESGFTEFSTPILTASSPEGARDF
CHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCHHC
LVPSRLHQGKFYALPQAPQIYKQLIMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCHHHHCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFSTMEPVMRGIFEAFAEGKPVTQKLPRIPYDEAMRKYGSDKPDLRNPIVME
EEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
EVSEHFRGSGFKVFANILANDPKAEVWAIPAKTGGSRAFCDRMNSWAQGEGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
RKEGDTLEGAGPIAKNIGPERTDAIRTQLGLGDGDACFFVAGDPKKFVAFAGAARTRAGE
ECCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHEECCHHHHCCCC
ELNLVDRDRFELAWIVDFPFYEWNEDEKRIEFGHNPFSMPQGGMEALENQEPLSIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCCEEEEEEE
DLVCNGFEIASGGIRNHMPELMVKAFEVAGFDRQMVEERFGGLYRAFQYGAPPHGGMAAG
EEEECCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRIVMLLRGAKNLREITMFPMNQQAMDLLMGAPSEATPQQLRELHIRLAPKQD
HHHHHHHHHCCCCHHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA