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The map label for this gene is proS

Identifier: 110633394

GI number: 110633394

Start: 1142139

End: 1143467

Strand: Direct

Name: proS

Synonym: Meso_1040

Alternate gene names: 110633394

Gene position: 1142139-1143467 (Clockwise)

Preceding gene: 110633393

Following gene: 110633395

Centisome position: 25.88

GC content: 59.44

Gene sequence:

>1329_bases
ATGCGTGTATCGCGTTACTTTTTGCCCATATTGAAGGAGACACCCCGCGAGGCTGAAATCGTCTCGCATAGGCTTATGCT
CCGCTCCGGCATGATCCGGCAGCAGGGGGCGGGCAGTTTCTCGTGGCTGCCTCTCGGCAAGCGCGTTCTCGACAAGGTGT
GCCAGATCGTCCGCGAGGAGCAGGATCGCGCGGGCGCCCAGGAAATCCTGATGCCCACCATTCAGTCGGCCGATCTATGG
ATCGAGAGCGGCCGTTACAACGATTATGGCAAGGAGATGCTGCGCATCCGCGACCGGCAGGATCGCGCCATGCTCTACGG
ACCCACCAATGAAGAGATGGTGACGGATATCTTCCGGGCCTATGTGAAATCCTACAAGGATCTGCCGCTCAATCTTTATC
ACATCCAATGGAAGTTCCGCGACGAGGTGCGGCCGCGTTTCGGCGTCATGCGCTCCCGTGAGTTCCTGATGAAGGACGCT
TACAGCTTCGACCTCGATTACGAGGGCGCCAAGGCGGCCTATAATCGCATGTTCGTGGCCTATCTTAGAACTTTCGCCCG
CATGGGCCTGAAGGCTATTCCCATGCGAGCCGATACGGGCCCGATCGGCGGCGATTTGAGCCACGAATTCATCATTCTCG
CCTCAACGGGCGAAAGCGAGGTTTTCTGCAATAAGGACTTTTTGGACCTTCCGGTGCCGCCGGCCGATGTCGATTTCACC
AATGATGCGGAGATCGGCGGCGTGGTTTCGGAGTGGACCACACCCTATGCGGCGACGGACGAGATGCATGACGAGGCCGC
ATGGGCCGAGGTGCCGGCGGAAAAGCAGCTTGCCGCGCGCGGCATTGAAGTTGGCCATATCTTCCATTTCGGCACGAAAT
ATTCCAAGCCGATGAACGCGAAGGTGACGGGGCCGGACGGGCAGGAACACTTCGTTTCCATGGGTTCCTATGGCATCGGC
CCGACGCGGCTTACCGCCGCGATTATCGAGGCGAGCCATGATGATGGCGGAATTATCTGGCCGGAAAGCGTGGCTCCCTT
CGATGTGGCGCTCATAAATATGAAAGTGGGCGATGCGGAATGTGACCGCGTTTGCGAGGAGCTATACGCCGGGATGACGG
CGGCCGGTCGCGAGGTGCTTTACGACGACACGGATCAGCGCGCCGGCGCGAAGTTTGCGACGGCTGATCTCATCGGTCTT
CCCTGGCAGGTAATCGTCGGCCCGCGTGGAATAGCCGCCGGAGAAGTTGAAGTGAAGAACCGAGCCACGGGAGAGCGTGA
GAATTTGCCTATTTCGGCAGTACCCGGGCGGTTCGGCGCGCGTCGATGA

Upstream 100 bases:

>100_bases
TGGATGGTGCGGGTTTTCAATTTGCCTCGAAACCGATAAGAAGCGTCACCGCTCTGGCGGGCAAGACTGATTCCGCCGCC
CTCTCGTCACGGATAAACTC

Downstream 100 bases:

>100_bases
GCGGAGCCGAGGCGGCAAAAGGGGGTATTAAACCCTTCTCCTATTTCGAGCGCCTCGTCGCTTGGCGCTATCTGCGCTCG
CGCCGCAAGGAAACCGTCGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRVSRYFLPILKETPREAEIVSHRLMLRSGMIRQQGAGSFSWLPLGKRVLDKVCQIVREEQDRAGAQEILMPTIQSADLW
IESGRYNDYGKEMLRIRDRQDRAMLYGPTNEEMVTDIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKAAYNRMFVAYLRTFARMGLKAIPMRADTGPIGGDLSHEFIILASTGESEVFCNKDFLDLPVPPADVDFT
NDAEIGGVVSEWTTPYAATDEMHDEAAWAEVPAEKQLAARGIEVGHIFHFGTKYSKPMNAKVTGPDGQEHFVSMGSYGIG
PTRLTAAIIEASHDDGGIIWPESVAPFDVALINMKVGDAECDRVCEELYAGMTAAGREVLYDDTDQRAGAKFATADLIGL
PWQVIVGPRGIAAGEVEVKNRATGERENLPISAVPGRFGARR

Sequences:

>Translated_442_residues
MRVSRYFLPILKETPREAEIVSHRLMLRSGMIRQQGAGSFSWLPLGKRVLDKVCQIVREEQDRAGAQEILMPTIQSADLW
IESGRYNDYGKEMLRIRDRQDRAMLYGPTNEEMVTDIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKAAYNRMFVAYLRTFARMGLKAIPMRADTGPIGGDLSHEFIILASTGESEVFCNKDFLDLPVPPADVDFT
NDAEIGGVVSEWTTPYAATDEMHDEAAWAEVPAEKQLAARGIEVGHIFHFGTKYSKPMNAKVTGPDGQEHFVSMGSYGIG
PTRLTAAIIEASHDDGGIIWPESVAPFDVALINMKVGDAECDRVCEELYAGMTAAGREVLYDDTDQRAGAKFATADLIGL
PWQVIVGPRGIAAGEVEVKNRATGERENLPISAVPGRFGARR
>Mature_442_residues
MRVSRYFLPILKETPREAEIVSHRLMLRSGMIRQQGAGSFSWLPLGKRVLDKVCQIVREEQDRAGAQEILMPTIQSADLW
IESGRYNDYGKEMLRIRDRQDRAMLYGPTNEEMVTDIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKAAYNRMFVAYLRTFARMGLKAIPMRADTGPIGGDLSHEFIILASTGESEVFCNKDFLDLPVPPADVDFT
NDAEIGGVVSEWTTPYAATDEMHDEAAWAEVPAEKQLAARGIEVGHIFHFGTKYSKPMNAKVTGPDGQEHFVSMGSYGIG
PTRLTAAIIEASHDDGGIIWPESVAPFDVALINMKVGDAECDRVCEELYAGMTAAGREVLYDDTDQRAGAKFATADLIGL
PWQVIVGPRGIAAGEVEVKNRATGERENLPISAVPGRFGARR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=414, Percent_Identity=35.024154589372, Blast_Score=241, Evalue=8e-64,
Organism=Escherichia coli, GI1786392, Length=262, Percent_Identity=53.0534351145038, Blast_Score=276, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI115532348, Length=402, Percent_Identity=32.3383084577114, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=36.2637362637363, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=259, Percent_Identity=33.5907335907336, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=423, Percent_Identity=32.1513002364066, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_MESSB (Q11JI8)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673602.1
- ProteinModelPortal:   Q11JI8
- SMR:   Q11JI8
- STRING:   Q11JI8
- GeneID:   4180398
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1040
- NMPDR:   fig|266779.1.peg.2541
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q11JI8
- ProtClustDB:   PRK12325
- BioCyc:   MSP266779:MESO_1040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49467; Mature: 49467

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRYFLPILKETPREAEIVSHRLMLRSGMIRQQGAGSFSWLPLGKRVLDKVCQIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHHHHHHHHHH
QDRAGAQEILMPTIQSADLWIESGRYNDYGKEMLRIRDRQDRAMLYGPTNEEMVTDIFRA
HHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
YVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDYEGAKAAYNRMFVA
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
YLRTFARMGLKAIPMRADTGPIGGDLSHEFIILASTGESEVFCNKDFLDLPVPPADVDFT
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
NDAEIGGVVSEWTTPYAATDEMHDEAAWAEVPAEKQLAARGIEVGHIFHFGTKYSKPMNA
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCC
KVTGPDGQEHFVSMGSYGIGPTRLTAAIIEASHDDGGIIWPESVAPFDVALINMKVGDAE
EEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEEECCCHH
CDRVCEELYAGMTAAGREVLYDDTDQRAGAKFATADLIGLPWQVIVGPRGIAAGEVEVKN
HHHHHHHHHCCHHHCCCEEEECCCCCCCCCCEEHHHHHCCCEEEEECCCCCCCCEEEECC
RATGERENLPISAVPGRFGARR
CCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRVSRYFLPILKETPREAEIVSHRLMLRSGMIRQQGAGSFSWLPLGKRVLDKVCQIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHHHHHHHHHH
QDRAGAQEILMPTIQSADLWIESGRYNDYGKEMLRIRDRQDRAMLYGPTNEEMVTDIFRA
HHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
YVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDYEGAKAAYNRMFVA
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
YLRTFARMGLKAIPMRADTGPIGGDLSHEFIILASTGESEVFCNKDFLDLPVPPADVDFT
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
NDAEIGGVVSEWTTPYAATDEMHDEAAWAEVPAEKQLAARGIEVGHIFHFGTKYSKPMNA
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCC
KVTGPDGQEHFVSMGSYGIGPTRLTAAIIEASHDDGGIIWPESVAPFDVALINMKVGDAE
EEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEEECCCHH
CDRVCEELYAGMTAAGREVLYDDTDQRAGAKFATADLIGLPWQVIVGPRGIAAGEVEVKN
HHHHHHHHHCCHHHCCCEEEECCCCCCCCCCEEHHHHHCCCEEEEECCCCCCCCEEEECC
RATGERENLPISAVPGRFGARR
CCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA