Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 110633377

GI number: 110633377

Start: 1123866

End: 1124438

Strand: Direct

Name: nuoB

Synonym: Meso_1023

Alternate gene names: 110633377

Gene position: 1123866-1124438 (Clockwise)

Preceding gene: 110633376

Following gene: 110633378

Centisome position: 25.47

GC content: 62.13

Gene sequence:

>573_bases
ATGGGATTGAACGATACCCTCGTTGCGCCCAGGTCGCGCGGTATCATTGACCCCAACACGGGCAACCCGGTCGGTGCGAA
TGATCCGTTTTACGGCGCGATCAATGAAGAGCTTTCCGACAAGGGCTTCCTTGTCACCTCCTCCGAGGCGCTGATCACCT
GGGCGCGTACGGGCTCGCTGATGTGGATGACCTTCGGTCTGGCCTGTTGCGCGGTGGAGATGATGCACACCTCCATGCCG
CGTTATGATGCAGAGCGTTTCGGTATCGCGCCGCGCGCCTCCCCGCGCCAGTCGGATGTGATGATCGTCGCCGGCACGCT
CACCAACAAGATGGCGCCGGCCCTGCGCAAGGTCTACGACCAGATGCCGGAGCCGCGCTACGTGATCTCCATGGGATCCT
GCGCCAATGGCGGCGGCTACTACCATTATTCCTATTCGGTGGTGCGCGGCTGCGATCGCATCGTGCCGGTGGATATCTAT
GTGCCCGGCTGCCCGCCCACCGCGGAAGCGCTGCTATACGGCATTCTTCTCCTTCAAAAGAAGATCCGCCGGACCGGCAC
CATCGAGCGCTAG

Upstream 100 bases:

>100_bases
CCTTCGGCGAGATCGGCTGGTTCGGCTTCTGGTCGATGATGATCTTCCTCGGCGTGCTGACCATCGGCTTCATCTATGAG
TGGAAGAAGGGAGCGCTGGA

Downstream 100 bases:

>100_bases
GAGCGGACCATGAATGAAGCGCTGAGGGATCTCGCGGGCTACATCAAGGAAAAGCTGGACGGCCGGGTCCAGGACTGGAT
GGTCGCCTATGACGAGCTGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MGLNDTLVAPRSRGIIDPNTGNPVGANDPFYGAINEELSDKGFLVTSSEALITWARTGSLMWMTFGLACCAVEMMHTSMP
RYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIY
VPGCPPTAEALLYGILLLQKKIRRTGTIER

Sequences:

>Translated_190_residues
MGLNDTLVAPRSRGIIDPNTGNPVGANDPFYGAINEELSDKGFLVTSSEALITWARTGSLMWMTFGLACCAVEMMHTSMP
RYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIY
VPGCPPTAEALLYGILLLQKKIRRTGTIER
>Mature_189_residues
GLNDTLVAPRSRGIIDPNTGNPVGANDPFYGAINEELSDKGFLVTSSEALITWARTGSLMWMTFGLACCAVEMMHTSMPR
YDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYV
PGCPPTAEALLYGILLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=162, Percent_Identity=74.0740740740741, Blast_Score=250, Evalue=5e-67,
Organism=Escherichia coli, GI1788624, Length=171, Percent_Identity=45.0292397660819, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=46.031746031746, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=43.4426229508197, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=146, Percent_Identity=67.8082191780822, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=78.4172661870504, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=78.4172661870504, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=78.0141843971631, Blast_Score=240, Evalue=3e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_MESSB (Q11JK5)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673585.1
- ProteinModelPortal:   Q11JK5
- SMR:   Q11JK5
- STRING:   Q11JK5
- GeneID:   4180381
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1023
- NMPDR:   fig|266779.1.peg.2558
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   HYDNERF
- PhylomeDB:   Q11JK5
- ProtClustDB:   PRK06411
- BioCyc:   MSP266779:MESO_1023-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20805; Mature: 20674

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNDTLVAPRSRGIIDPNTGNPVGANDPFYGAINEELSDKGFLVTSSEALITWARTGSL
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCC
MWMTFGLACCAVEMMHTSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYD
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILLLQK
HCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRRTGTIER
HHHHCCCCCC
>Mature Secondary Structure 
GLNDTLVAPRSRGIIDPNTGNPVGANDPFYGAINEELSDKGFLVTSSEALITWARTGSL
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCC
MWMTFGLACCAVEMMHTSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYD
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILLLQK
HCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRRTGTIER
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA