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The map label for this gene is dnaB [H]

Identifier: 110633341

GI number: 110633341

Start: 1083028

End: 1084518

Strand: Reverse

Name: dnaB [H]

Synonym: Meso_0987

Alternate gene names: 110633341

Gene position: 1084518-1083028 (Counterclockwise)

Preceding gene: 110633342

Following gene: 110633340

Centisome position: 24.58

GC content: 61.5

Gene sequence:

>1491_bases
ATGGCCGACGCAGTCCGCAAGTTGAATCCGGCGGAGACGCCGCTTTATCGGGAGCCGCCGAACAATATCGAGGCCGAACA
GGCCTTGCTCGGAGCGATCCTGGTCAACAATGATGCTTTCTACCGGGTTTCGGATTTCCTGAAACCAAACCACTTCTATG
AGCCCCTGCACCGGCGGATCTATGAGATCGCGGCGGAGTTCATCCGCATGGGCAAGATCGCAAGCCCCATCACCATCAAG
ACATTTCTGCCGGCGGAAGAAAAAGTAGGCGAACTGACGGTCGCCCAGTATCTGGCGCGTCTTGCCGCCGAGGCCGTCAC
CATCATCAACGCCGCCGACTACGGCCGCGCCATCTATGATCTTGCCGTCCGCAGGGCGCTGATCACCGTTGGCGAGGACA
TGGTCAACATCGCCTATGACGCCCCCGTGGACATGGCCCCGCAGTCTCAGATCGAGGACGCGGAGCGCCGGCTTTTCGAA
CTGGCGGAGACCGGCCGCTACGATGGCGGCTTCGAAAGCTTCGGGGACGCCACCAAGACGGCCATCGACATGGCCAACGC
CGCCTTCATGCGCGATGGAGGCCTTTCCGGCATTTCAACGGGCCTGCGCGATCTCGACCGGCGCATGGGCGGGCTGCAGC
CCTCCGACTTGATCATTCTCGCCGGGCGTCCGGGCATGGGAAAGACCTCGCTCGCCACCAACATCGCCTTCAACATCGCT
GCCGCCTATGAGCCTGCGCAACAGGCGGATGGCTCCTTTAAGGCCGCCAATGGTGGCGTCGTCGGCTTCTTCTCGCTCGA
AATGTCGTCAGAGCAGCTCGCCACGCGTATCATCTCCGAGCAGACCGAGATCCCCTCCTCCAAGATCCGCCGTGGCGAGA
TTACGGAAGCGGAGTTCGAGAAGCTTGTGGCTTGCGCGCAGACGATGCAGAAGATCCCGCTCTTTATCGATGCGACCGGC
GGCATTTCCATTGCGCAGCTTGCCGCGCGATCGAGGCGGCTCAAGCGTCAGCGCGGGCTCGACGTTCTGGTCATCGACTA
TGTGCAGCTCATGCAGGGTTCCTCCGCCAAGTCGCAGCAGAACCGCGTGCAGGAAATCACGGAGATCACTACCGGCCTTA
AGGCGCTCGCCAAGGAGCTTGGCGTACCGATCATCGCGCTCTCCCAGCTCTCGCGTCAGGTGGAAAGCCGCGACGACAAG
CGGCCGCAGCTTTCGGACCTGCGCGAATCGGGCTCCATCGAGCAGGACGCCGACGTGGTGCTGTTCGTCTACCGCGAAGA
ATACTATCTCAAGAATAAGGAGCCCAAGCCAGGCTCGGAAGAATATCTGAGATGGGAAGCGGATATGAACGAGGTGCGCG
GCAAGTCCGAGGTGATCATTGCCAAGCAGCGCCACGGGCCTACGGGAATGGTGCCCCTTGGCTTCCAGGGCGAGTTCACG
CGCTTCTTCGACCTTGCGGAGGATTCGCACCTGCCGGAGCGTTTCGAATAG

Upstream 100 bases:

>100_bases
TCACTTACATCACCGCCCCCGAGAGGTTATGAGAATGCTCAAGGGGGGCGCCGGACCGTATCAGCTGTTTTCCGGCCGCC
ATTCGTAAGATGGAAATGTT

Downstream 100 bases:

>100_bases
CGGTGCCTGTTCCGTCGCATATATCGGATACAGGCCCGCCGCCTCATCTGGCGGGCGGGCGACTGACGGTCGACCTTTCC
GCGCTTGTGGCGAATTACCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MADAVRKLNPAETPLYREPPNNIEAEQALLGAILVNNDAFYRVSDFLKPNHFYEPLHRRIYEIAAEFIRMGKIASPITIK
TFLPAEEKVGELTVAQYLARLAAEAVTIINAADYGRAIYDLAVRRALITVGEDMVNIAYDAPVDMAPQSQIEDAERRLFE
LAETGRYDGGFESFGDATKTAIDMANAAFMRDGGLSGISTGLRDLDRRMGGLQPSDLIILAGRPGMGKTSLATNIAFNIA
AAYEPAQQADGSFKAANGGVVGFFSLEMSSEQLATRIISEQTEIPSSKIRRGEITEAEFEKLVACAQTMQKIPLFIDATG
GISIAQLAARSRRLKRQRGLDVLVIDYVQLMQGSSAKSQQNRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDK
RPQLSDLRESGSIEQDADVVLFVYREEYYLKNKEPKPGSEEYLRWEADMNEVRGKSEVIIAKQRHGPTGMVPLGFQGEFT
RFFDLAEDSHLPERFE

Sequences:

>Translated_496_residues
MADAVRKLNPAETPLYREPPNNIEAEQALLGAILVNNDAFYRVSDFLKPNHFYEPLHRRIYEIAAEFIRMGKIASPITIK
TFLPAEEKVGELTVAQYLARLAAEAVTIINAADYGRAIYDLAVRRALITVGEDMVNIAYDAPVDMAPQSQIEDAERRLFE
LAETGRYDGGFESFGDATKTAIDMANAAFMRDGGLSGISTGLRDLDRRMGGLQPSDLIILAGRPGMGKTSLATNIAFNIA
AAYEPAQQADGSFKAANGGVVGFFSLEMSSEQLATRIISEQTEIPSSKIRRGEITEAEFEKLVACAQTMQKIPLFIDATG
GISIAQLAARSRRLKRQRGLDVLVIDYVQLMQGSSAKSQQNRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDK
RPQLSDLRESGSIEQDADVVLFVYREEYYLKNKEPKPGSEEYLRWEADMNEVRGKSEVIIAKQRHGPTGMVPLGFQGEFT
RFFDLAEDSHLPERFE
>Mature_495_residues
ADAVRKLNPAETPLYREPPNNIEAEQALLGAILVNNDAFYRVSDFLKPNHFYEPLHRRIYEIAAEFIRMGKIASPITIKT
FLPAEEKVGELTVAQYLARLAAEAVTIINAADYGRAIYDLAVRRALITVGEDMVNIAYDAPVDMAPQSQIEDAERRLFEL
AETGRYDGGFESFGDATKTAIDMANAAFMRDGGLSGISTGLRDLDRRMGGLQPSDLIILAGRPGMGKTSLATNIAFNIAA
AYEPAQQADGSFKAANGGVVGFFSLEMSSEQLATRIISEQTEIPSSKIRRGEITEAEFEKLVACAQTMQKIPLFIDATGG
ISIAQLAARSRRLKRQRGLDVLVIDYVQLMQGSSAKSQQNRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDKR
PQLSDLRESGSIEQDADVVLFVYREEYYLKNKEPKPGSEEYLRWEADMNEVRGKSEVIIAKQRHGPTGMVPLGFQGEFTR
FFDLAEDSHLPERFE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=475, Percent_Identity=35.3684210526316, Blast_Score=281, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54897; Mature: 54766

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAVRKLNPAETPLYREPPNNIEAEQALLGAILVNNDAFYRVSDFLKPNHFYEPLHRRI
CCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEHHHHCCCCHHHHHHHHHH
YEIAAEFIRMGKIASPITIKTFLPAEEKVGELTVAQYLARLAAEAVTIINAADYGRAIYD
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAVRRALITVGEDMVNIAYDAPVDMAPQSQIEDAERRLFELAETGRYDGGFESFGDATKT
HHHHHHHHHHCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHH
AIDMANAAFMRDGGLSGISTGLRDLDRRMGGLQPSDLIILAGRPGMGKTSLATNIAFNIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHEEEH
AAYEPAQQADGSFKAANGGVVGFFSLEMSSEQLATRIISEQTEIPSSKIRRGEITEAEFE
HHCCCHHHCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
KLVACAQTMQKIPLFIDATGGISIAQLAARSRRLKRQRGLDVLVIDYVQLMQGSSAKSQQ
HHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHH
NRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEE
LFVYREEYYLKNKEPKPGSEEYLRWEADMNEVRGKSEVIIAKQRHGPTGMVPLGFQGEFT
EEEEECHHHHCCCCCCCCCHHHEEECCCHHHHCCCCEEEEEECCCCCCCEEECCCCCCHH
RFFDLAEDSHLPERFE
HHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADAVRKLNPAETPLYREPPNNIEAEQALLGAILVNNDAFYRVSDFLKPNHFYEPLHRRI
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEHHHHCCCCHHHHHHHHHH
YEIAAEFIRMGKIASPITIKTFLPAEEKVGELTVAQYLARLAAEAVTIINAADYGRAIYD
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAVRRALITVGEDMVNIAYDAPVDMAPQSQIEDAERRLFELAETGRYDGGFESFGDATKT
HHHHHHHHHHCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHH
AIDMANAAFMRDGGLSGISTGLRDLDRRMGGLQPSDLIILAGRPGMGKTSLATNIAFNIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHEEEH
AAYEPAQQADGSFKAANGGVVGFFSLEMSSEQLATRIISEQTEIPSSKIRRGEITEAEFE
HHCCCHHHCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
KLVACAQTMQKIPLFIDATGGISIAQLAARSRRLKRQRGLDVLVIDYVQLMQGSSAKSQQ
HHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHH
NRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEE
LFVYREEYYLKNKEPKPGSEEYLRWEADMNEVRGKSEVIIAKQRHGPTGMVPLGFQGEFT
EEEEECHHHHCCCCCCCCCHHHEEECCCHHHHCCCCEEEEEECCCCCCCEEECCCCCCHH
RFFDLAEDSHLPERFE
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA