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The map label for this gene is lysX [H]

Identifier: 110633311

GI number: 110633311

Start: 1054797

End: 1056257

Strand: Direct

Name: lysX [H]

Synonym: Meso_0957

Alternate gene names: 110633311

Gene position: 1054797-1056257 (Clockwise)

Preceding gene: 110633310

Following gene: 110633318

Centisome position: 23.91

GC content: 61.46

Gene sequence:

>1461_bases
ATGAGCTGGGTCATTCTCACCGGCAGACAAAGCGACCTCGACCAGGTCGCAACCCCGCACAAGATCATAACCAGCCGCGA
TTACCTGGCACATCCGGCCCTTTTCCGGGGACAGCGGCCCAAGATCATCAACCTGTCGAACAGCTACGCCTATCAGAGCC
GCGGCTATTATGCCTCGCTGCTGGCGAGCTCGCGCGGCCACAAGGTGATTCCCTCGGTCGAAACGATGATCGATCTTTCC
GGCCGCAAGCTCTACGAAAACGCGCTGCCGGAACTCGAACAGGCGCTCAACAGGTGCCGTAAGGAGCTGGGAGGAGGCTT
CCCGTCCACCGTGGCGGTGTTTTTCGGCATCGGCCCCTCCAAAGCCTGGGACCGTTTCGCGCGCCTGCTATTCGATTGGT
TCCGCGCGCCGGCGCTGGAGGTGGCGATCGAGGAAGGCGAATGGGCGACCATCAAGAAGATCGGCTTCCTGCCGCTGGCG
CGCATGAAGAACGACGAGAAGGCGCGGTTCCTCGCCAGCCTCGACAATTACACGAACCGGCAGTGGCGGGACACCAAAAC
GCGTACGCCCTCGCGCTATACCTTCGCCACTCTGGTGGACCCGCAGGAGGTGCTGCCGCCTTCTAGCATCGGCTCGCTCA
AGCACTGGTCGCGGATCGCCGAGAAAATGGGCGTGGAGGTGGAGCCGATCACGAGAAAGGACCTGCCGAAGCTCGCCAAC
TATGATGCGCTGTTCATCCGCGAGACGACCTCCATCTCCACTCACACCTACCGTTTCGCCCGCCGGGCGCAGCAGGAGGG
CATGCCGGTCATCGACGATCCGCTGTCAATGATACGCTGCACCAACAAGGTCTATCTGAACGAGCTGATGCTCTATAACC
GCGTATCCGTGCCGCCGACGGTGATGATTTCCGGGCCGGGGGATCTGGAGACAGCCGCCGACACGCTTGGGTTTCCACTC
GTGCTCAAGATCCCCGACAGCGCTTTTTCACGCGGTGTGAAGAAGGCGGGGAATCTGGCGGAGCTGAAAGAGCTCGCCAC
CAAGTGGCTGGAGGATTCCGATCTGCTAATCGCGCAGAAATTCCTGCCTACCGAGTTTGACTGGCGCATCGGCGTGCTGG
GCGGCGAGCCGCTTTTTGCGGTGCAGTATCTAATGGCGAAGAAGCATTGGCAGATCGTCAATCACGAGCGGCGCGGCAAG
CCGGACGAGGGCGGATTCCGCACCTTTACCCTGCAGGAAACCCCGCGCGAGGTGATCGATATCGCCGTGAAGGCTGCCCG
CTGTATCGGGGACGGGCTCTACGGCGTCGATATCAAGGAGACGCCGGACGGGATCTACGTGATCGAGGTGAACGACAATC
CCAACCTGGACCACGGCTGCGAGGATTCGGGCGAGAAGGACCAGGTCTGGATGCGGCTCACCCAATGGTTCATCGACCGG
CTGGAACGGCAGGGGAGATAA

Upstream 100 bases:

>100_bases
GGCGCTCGGGCTTCCGCCCCATCGGGCGCCGCGAAAACTATTACGAGGACGGTGCCCCGGCACTGCGTTTCGAAAAGCAT
CTGGGAGGCTCCAAGGAGCA

Downstream 100 bases:

>100_bases
ATCACGCTGCCTTGCGTCGCGACTTCTCGACGGCCTCGAAGCGGGCGAGATATGAAGCCTGGGCGCGCCGGGCGGGTTTC
GGCTCAAGATCAAGCGTTTC

Product: RimK-like ATP-grasp

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSWVILTGRQSDLDQVATPHKIITSRDYLAHPALFRGQRPKIINLSNSYAYQSRGYYASLLASSRGHKVIPSVETMIDLS
GRKLYENALPELEQALNRCRKELGGGFPSTVAVFFGIGPSKAWDRFARLLFDWFRAPALEVAIEEGEWATIKKIGFLPLA
RMKNDEKARFLASLDNYTNRQWRDTKTRTPSRYTFATLVDPQEVLPPSSIGSLKHWSRIAEKMGVEVEPITRKDLPKLAN
YDALFIRETTSISTHTYRFARRAQQEGMPVIDDPLSMIRCTNKVYLNELMLYNRVSVPPTVMISGPGDLETAADTLGFPL
VLKIPDSAFSRGVKKAGNLAELKELATKWLEDSDLLIAQKFLPTEFDWRIGVLGGEPLFAVQYLMAKKHWQIVNHERRGK
PDEGGFRTFTLQETPREVIDIAVKAARCIGDGLYGVDIKETPDGIYVIEVNDNPNLDHGCEDSGEKDQVWMRLTQWFIDR
LERQGR

Sequences:

>Translated_486_residues
MSWVILTGRQSDLDQVATPHKIITSRDYLAHPALFRGQRPKIINLSNSYAYQSRGYYASLLASSRGHKVIPSVETMIDLS
GRKLYENALPELEQALNRCRKELGGGFPSTVAVFFGIGPSKAWDRFARLLFDWFRAPALEVAIEEGEWATIKKIGFLPLA
RMKNDEKARFLASLDNYTNRQWRDTKTRTPSRYTFATLVDPQEVLPPSSIGSLKHWSRIAEKMGVEVEPITRKDLPKLAN
YDALFIRETTSISTHTYRFARRAQQEGMPVIDDPLSMIRCTNKVYLNELMLYNRVSVPPTVMISGPGDLETAADTLGFPL
VLKIPDSAFSRGVKKAGNLAELKELATKWLEDSDLLIAQKFLPTEFDWRIGVLGGEPLFAVQYLMAKKHWQIVNHERRGK
PDEGGFRTFTLQETPREVIDIAVKAARCIGDGLYGVDIKETPDGIYVIEVNDNPNLDHGCEDSGEKDQVWMRLTQWFIDR
LERQGR
>Mature_485_residues
SWVILTGRQSDLDQVATPHKIITSRDYLAHPALFRGQRPKIINLSNSYAYQSRGYYASLLASSRGHKVIPSVETMIDLSG
RKLYENALPELEQALNRCRKELGGGFPSTVAVFFGIGPSKAWDRFARLLFDWFRAPALEVAIEEGEWATIKKIGFLPLAR
MKNDEKARFLASLDNYTNRQWRDTKTRTPSRYTFATLVDPQEVLPPSSIGSLKHWSRIAEKMGVEVEPITRKDLPKLANY
DALFIRETTSISTHTYRFARRAQQEGMPVIDDPLSMIRCTNKVYLNELMLYNRVSVPPTVMISGPGDLETAADTLGFPLV
LKIPDSAFSRGVKKAGNLAELKELATKWLEDSDLLIAQKFLPTEFDWRIGVLGGEPLFAVQYLMAKKHWQIVNHERRGKP
DEGGFRTFTLQETPREVIDIAVKAARCIGDGLYGVDIKETPDGIYVIEVNDNPNLDHGCEDSGEKDQVWMRLTQWFIDRL
ERQGR

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW (Probable) [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 55154; Mature: 55023

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWVILTGRQSDLDQVATPHKIITSRDYLAHPALFRGQRPKIINLSNSYAYQSRGYYASL
CCEEEEECCCCCHHHHCCCHHHHCCCCHHHCCHHHCCCCCCEEECCCCCEECCCCHHHHH
LASSRGHKVIPSVETMIDLSGRKLYENALPELEQALNRCRKELGGGFPSTVAVFFGIGPS
HHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCC
KAWDRFARLLFDWFRAPALEVAIEEGEWATIKKIGFLPLARMKNDEKARFLASLDNYTNR
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHHCCEEHHHCCCCHHHHHHHHHHHHCCC
QWRDTKTRTPSRYTFATLVDPQEVLPPSSIGSLKHWSRIAEKMGVEVEPITRKDLPKLAN
CCCCCCCCCCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCEECCCCHHCCHHHCC
YDALFIRETTSISTHTYRFARRAQQEGMPVIDDPLSMIRCTNKVYLNELMLYNRVSVPPT
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
VMISGPGDLETAADTLGFPLVLKIPDSAFSRGVKKAGNLAELKELATKWLEDSDLLIAQK
EEEECCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHH
FLPTEFDWRIGVLGGEPLFAVQYLMAKKHWQIVNHERRGKPDEGGFRTFTLQETPREVID
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEECCCHHHHHH
IAVKAARCIGDGLYGVDIKETPDGIYVIEVNDNPNLDHGCEDSGEKDQVWMRLTQWFIDR
HHHHHHHHHCCCCEECCCEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LERQGR
HHHCCC
>Mature Secondary Structure 
SWVILTGRQSDLDQVATPHKIITSRDYLAHPALFRGQRPKIINLSNSYAYQSRGYYASL
CEEEEECCCCCHHHHCCCHHHHCCCCHHHCCHHHCCCCCCEEECCCCCEECCCCHHHHH
LASSRGHKVIPSVETMIDLSGRKLYENALPELEQALNRCRKELGGGFPSTVAVFFGIGPS
HHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCC
KAWDRFARLLFDWFRAPALEVAIEEGEWATIKKIGFLPLARMKNDEKARFLASLDNYTNR
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHHCCEEHHHCCCCHHHHHHHHHHHHCCC
QWRDTKTRTPSRYTFATLVDPQEVLPPSSIGSLKHWSRIAEKMGVEVEPITRKDLPKLAN
CCCCCCCCCCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCEECCCCHHCCHHHCC
YDALFIRETTSISTHTYRFARRAQQEGMPVIDDPLSMIRCTNKVYLNELMLYNRVSVPPT
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
VMISGPGDLETAADTLGFPLVLKIPDSAFSRGVKKAGNLAELKELATKWLEDSDLLIAQK
EEEECCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHH
FLPTEFDWRIGVLGGEPLFAVQYLMAKKHWQIVNHERRGKPDEGGFRTFTLQETPREVID
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEECCCHHHHHH
IAVKAARCIGDGLYGVDIKETPDGIYVIEVNDNPNLDHGCEDSGEKDQVWMRLTQWFIDR
HHHHHHHHHCCCCEECCCEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LERQGR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726; 12042311 [H]